LOCUS       BBa_K116648             2014 bp    DNA     linear       21-Nov-2024
DEFINITION  Intermediate part to construct a synchronizer
ACCESSION   BBa_K116648
VERSION     BBa_K116648.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..75
                     /label=RBS-3\Weak
     RBS             69..72
                     /label=RBS
     misc_feature    63..75
                     /label=BBa_B0032
     RBS             63..75
                     /label=BBa_B0032
     misc_feature    82..83
                     /label=prefix
     misc_feature    255..255
                     /label=T
     misc_feature    573..573
                     /label=A
     CDS             82..831
                     /label=luxR
     misc_feature    82..837
                     /label=BBa_C0062
     misc_feature    838..862
                     /label=Help:Barcodes
     CDS             82..837
                     /label=BBa_C0062
     misc_feature    63..862
                     /label=BBa_K116616
     stem_loop       882..925
                     /label=stem_loop
     misc_feature    871..950
                     /label=BBa_B0010
     terminator      871..950
                     /label=BBa_B0010
     stem_loop       966..985
                     /label=T7 TE
     misc_feature    992..992
                     /label=stop
     polyA_site      986..999
                     /label=polya
     misc_feature    959..999
                     /label=BBa_B0012
     terminator      959..999
                     /label=BBa_B0012
     terminator      871..999
                     /label=BBa_B0015
     misc_feature    63..999
                     /label=BBa_K116626
     misc_feature    1..999
                     /label=BBa_K116640
     CDS             1008..1095
                     /label=end of LacI coding region (inactive)
     misc_feature    1008..1207
                     /label=BBa_R0010
     misc_feature    1096..1133
                     /label=CAP binding site
     promoter        1144..1149
                     /label=-35
     promoter        1168..1173
                     /label=-10
     misc_feature    1173..1207
                     /label=LacI binding site
     misc_feature    1180..1180
                     /label=start
     promoter        1008..1207
                     /label=BBa_R0010
     RBS             1216..1228
                     /label=RBS-3\Weak
     RBS             1222..1225
                     /label=RBS
     misc_feature    1216..1228
                     /label=BBa_B0032
     RBS             1216..1228
                     /label=BBa_B0032
     misc_feature    1814..1845
                     /label=LVA
     CDS             1235..1813
                     /label=luxI
     misc_feature    1235..1852
                     /label=BBa_C0061
     misc_feature    1853..1877
                     /label=Help:Barcodes
     CDS             1235..1852
                     /label=BBa_C0061
     stem_loop       1897..1940
                     /label=stem_loop
     misc_feature    1886..1965
                     /label=BBa_B0010
     terminator      1886..1965
                     /label=BBa_B0010
     stem_loop       1981..2000
                     /label=T7 TE
     misc_feature    2007..2007
                     /label=stop
     polyA_site      2001..2014
                     /label=polya
     misc_feature    1974..2014
                     /label=BBa_B0012
     terminator      1974..2014
                     /label=BBa_B0012
     terminator      1886..2014
                     /label=BBa_B0015
     misc_feature    1235..2014
                     /label=BBa_K116615
     misc_feature    1216..2014
                     /label=BBa_K116625
     misc_feature    1008..2014
                     /label=BBa_K116638
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaagtacta gatgaaaaac ataaatgccg acgacacata cagaataatt
      121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact
      181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa
      241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct
      301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat
      361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg
      421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc
      481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat
      541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat
      601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg
      661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact
      721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt
      781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac
      841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt
      901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      961 acactggctc accttcgggt gggcctttct gcgtttatat actagagcaa tacgcaaacc
     1021 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     1081 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     1141 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     1201 tcacacatac tagagtcaca caggaaagta ctagatgact ataatgataa aaaaatcgga
     1261 ttttttggca attccatcgg aggagtataa aggtattcta agtcttcgtt atcaagtgtt
     1321 taagcaaaga cttgagtggg acttagttgt agaaaataac cttgaatcag atgagtatga
     1381 taactcaaat gcagaatata tttatgcttg tgatgatact gaaaatgtaa gtggatgctg
     1441 gcgtttatta cctacaacag gtgattatat gctgaaaagt gtttttcctg aattgcttgg
     1501 tcaacagagt gctcccaaag atcctaatat agtcgaatta agtcgttttg ctgtaggtaa
     1561 aaatagctca aagataaata actctgctag tgaaattaca atgaaactat ttgaagctat
     1621 atataaacac gctgttagtc aaggtattac agaatatgta acagtaacat caacagcaat
     1681 agagcgattt ttaaagcgta ttaaagttcc ttgtcatcgt attggagaca aagaaattca
     1741 tgtattaggt gatactaaat cggttgtatt gtctatgcct attaatgaac agtttaaaaa
     1801 agcagtctta aatgctgcaa acgacgaaaa ctacgcttta gtagcttaat aactctgata
     1861 gtgctagtgt agatctctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     1921 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     1981 ggctcacctt cgggtgggcc tttctgcgtt tata
//