LOCUS BBa_K116651 3601 bp DNA linear 05-Nov-2024 DEFINITION Oscillator Reporter ACCESSION BBa_K116651 VERSION BBa_K116651.1 FEATURES Location/Qualifiers promoter 1..48 /label=BBa_K116603 RBS 57..69 /label=RBS-3\Weak RBS 63..66 /label=RBS misc_feature 57..69 /label=BBa_B0032 RBS 57..69 /label=BBa_B0032 CDS 76..2010 /label=BBa_K116601 stem_loop 2030..2073 /label=stem_loop misc_feature 2019..2098 /label=BBa_B0010 terminator 2019..2098 /label=BBa_B0010 stem_loop 2114..2133 /label=T7 TE misc_feature 2140..2140 /label=stop polyA_site 2134..2147 /label=polya misc_feature 2107..2147 /label=BBa_B0012 terminator 2107..2147 /label=BBa_B0012 promoter 2156..2203 /label=BBa_K116603 RBS 2212..2224 /label=RBS-3\Weak RBS 2218..2221 /label=RBS misc_feature 2212..2224 /label=BBa_B0032 RBS 2212..2224 /label=BBa_B0032 CDS 2231..2524 /label=BBa_K116602 stem_loop 2544..2587 /label=stem_loop misc_feature 2533..2612 /label=BBa_B0010 terminator 2533..2612 /label=BBa_B0010 stem_loop 2628..2647 /label=T7 TE misc_feature 2654..2654 /label=stop polyA_site 2648..2661 /label=polya misc_feature 2621..2661 /label=BBa_B0012 terminator 2621..2661 /label=BBa_B0012 promoter 2670..2717 /label=BBa_K116603 RBS 2726..2738 /label=RBS-3\Weak RBS 2732..2735 /label=RBS misc_feature 2726..2738 /label=BBa_B0032 RBS 2726..2738 /label=BBa_B0032 CDS 2745..3464 /label=GFP protein CDS 2745..3464 /label=BBa_E0040 stem_loop 3484..3527 /label=stem_loop misc_feature 3473..3552 /label=BBa_B0010 terminator 3473..3552 /label=BBa_B0010 stem_loop 3568..3587 /label=T7 TE misc_feature 3594..3594 /label=stop polyA_site 3588..3601 /label=polya misc_feature 3561..3601 /label=BBa_B0012 terminator 3561..3601 /label=BBa_B0012 ORIGIN 1 agagcctcgt tgcgtttgtt tgcacgaacc atatgtaagt atttccttta ctagagtcac 61 acaggaaagt actagatggc gaaaaaccta atactctggc tggtcattgc cgttgtgctg 121 atgtcagtat tccagagctt tgggcccagc gagtctaatg gccgtaaggt ggattactct 181 accttcctac aagaggtcaa taacgaccag gttcgtgaag cgcgtatcaa cggacgtgaa 241 atcaacgtta ccaagaaaga tagtaaccgt tataccactt acattccggt tcaggatccg 301 aaattactgg ataacctgtt gaccaagaac gtcaaggttg tcggtgaacc gcctgaagaa 361 ccaagcctgc tggcttctat cttcatctcc tggttcccga tgctgttgct gattggtgtc 421 tggatcttct tcatgcgtca aatgcagggc ggcggtggca aaggtgccat gtcgtttggt 481 aagagcaaag cgcgcatgct gacggaagat cagatcaaaa cgacctttgc tgacgttgcg 541 ggctgcgacg aagcaaaaga agaagttgct gaactggttg agtatctgcg cgagccgagc 601 cgcttccaga aactcggcgg taagatcccg aaaggcgtct tgatggtcgg tcctccgggt 661 accggtaaaa cgctgctggc gaaagcgatt gcaggcgaag cgaaagttcc gttctttact 721 atctccggtt ctgacttcgt agaaatgttc gtcggtgtgg gtgcatcccg tgttcgtgac 781 atgttcgaac aggcgaagaa agcggcaccg tgcatcatct ttatcgatga aatcgacgcc 841 gtaggccgcc agcgtggcgc tggtctgggc ggtggtcacg atgaacgtga acagactctg 901 aaccagatgc tggttgagat ggatggcttc gaaggtaacg aaggtatcat cgttatcgcc 961 gcgactaacc gtccggacgt tctcgacccg gccctgctgc gtcctggccg tttcgaccgt 1021 caggttgtgg tcggcttgcc agatgttcgc ggtcgtgagc agatcctgaa agttcacatg 1081 cgtcgcgtac cattggcacc cgatatcgac gcggcaatca ttgcccgtgg tactcctggt 1141 ttctccggtg ctgacctggc gaacctggtg aacgaagcgg cactgttcgc tgctcgtggc 1201 aacaaacgcg ttgtgtcgat ggttgagttc gagaaagcga aagacaaaat catgatgggt 1261 gcggaacgtc gctccatggt gatgacggaa gcgcagaaag aatcgacggc ttaccacgaa 1321 gcgggtcatg cgattatcgg tcgcctggtg ccggaacacg atccggtgca caaagtgacg 1381 attatcccac gcggtcgtgc gctgggtgtg actttcttct tgcctgaggg cgacgcaatc 1441 agcgccagcc gtcagaaact ggaaagccag atttctacgc tgtacggtgg tcgtctggca 1501 gaagagatca tctacgggcc ggaacatgta tctaccggtg cgtccaacga tattaaagtt 1561 gcgaccaacc tggcacgtaa catggtgact cagtggggct tctctgagaa attgggtcca 1621 ctgctgtacg cggaagaaga aggtgaagtg ttcctcggcc gtagcgtagc gaaagcgaaa 1681 catatgtccg atgaaactgc acgtatcatc gaccaggaag tgaaagcact gattgagcgt 1741 aactataatc gtgcgcgtca gcttctgacc gacaatatgg atattctgca tgcgatgaaa 1801 gatgctctca tgaaatatga gactatcgac gcaccgcaga ttgatgacct gatggcacgt 1861 cgcgatgtac gtccgccagc gggctgggaa gaaccaggcg cttctaacaa ttctggcgac 1921 aatggtagtc caaaggctcc tcgtccggtt gatgaaccgc gtacgccgaa cccgggtaac 1981 accatgtcag agcagttagg cgacaagtaa tactagagcc aggcatcaaa taaaacgaaa 2041 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 2101 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagagagc 2161 ctcgttgcgt ttgtttgcac gaaccatatg taagtatttc ctttactaga gtcacacagg 2221 aaagtactag atggttcgtg caaacaaacg caacgaggct ctacgaatcg agagtgcgtt 2281 gcttaacaaa atcgcaatgc ttggaactga gaagacagcg gaagctgtgg gcgttgataa 2341 gtcgcagatc agcaggtgga agagggactg gattccaaag ttctcaatgc tgcttgctgt 2401 tcttgaatgg ggggtcgttg acgacgacat ggctcgattg gcgcgacaag ttgctgcgat 2461 tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt 2521 ctgatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 2581 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 2641 gtgggccttt ctgcgtttat atactagaga gagcctcgtt gcgtttgttt gcacgaacca 2701 tatgtaagta tttcctttac tagagtcaca caggaaagta ctagatgcgt aaaggagaag 2761 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca 2821 aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat 2881 ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt 2941 atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga 3001 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact 3061 acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa 3121 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata 3181 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca 3241 aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata 3301 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg 3361 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag 3421 ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag agccaggcat 3481 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 3541 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 3601 a //