LOCUS BBa_K116656 2383 bp DNA linear 18-Nov-2024 DEFINITION Synchronizer ACCESSION BBa_K116656 VERSION BBa_K116656.1 FEATURES Location/Qualifiers promoter 1..48 /label=BBa_K116603 RBS 57..69 /label=RBS-3\Weak RBS 63..66 /label=RBS misc_feature 57..69 /label=BBa_B0032 RBS 57..69 /label=BBa_B0032 misc_feature 655..686 /label=LVA CDS 76..654 /label=luxI misc_feature 76..693 /label=BBa_C0061 misc_feature 694..718 /label=Help:Barcodes CDS 76..693 /label=BBa_C0061 stem_loop 738..781 /label=stem_loop misc_feature 727..806 /label=BBa_B0010 terminator 727..806 /label=BBa_B0010 stem_loop 822..841 /label=T7 TE misc_feature 848..848 /label=stop polyA_site 842..855 /label=polya misc_feature 815..855 /label=BBa_B0012 terminator 815..855 /label=BBa_B0012 terminator 727..855 /label=BBa_B0015 misc_feature 76..855 /label=BBa_K116615 misc_feature 57..855 /label=BBa_K116625 misc_feature 1..855 /label=BBa_K116635 misc_feature 864..882 /label=TetR 1 misc_feature 864..917 /label=BBa_R0040 promoter 883..888 /label=-35 misc_feature 889..907 /label=TetR 2 promoter 906..911 /label=-10 promoter 864..917 /label=BBa_R0040 RBS 926..938 /label=RBS-3\Weak RBS 932..935 /label=RBS misc_feature 926..938 /label=BBa_B0032 RBS 926..938 /label=BBa_B0032 misc_feature 945..946 /label=prefix misc_feature 1118..1118 /label=T misc_feature 1436..1436 /label=A CDS 945..1694 /label=luxR misc_feature 945..1700 /label=BBa_C0062 misc_feature 1701..1725 /label=Help:Barcodes CDS 945..1700 /label=BBa_C0062 misc_feature 926..1725 /label=BBa_K116616 stem_loop 1745..1788 /label=stem_loop misc_feature 1734..1813 /label=BBa_B0010 terminator 1734..1813 /label=BBa_B0010 stem_loop 1829..1848 /label=T7 TE misc_feature 1855..1855 /label=stop polyA_site 1849..1862 /label=polya misc_feature 1822..1862 /label=BBa_B0012 terminator 1822..1862 /label=BBa_B0012 terminator 1734..1862 /label=BBa_B0015 misc_feature 926..1862 /label=BBa_K116626 misc_feature 864..1862 /label=BBa_K116640 misc_feature 1871..1890 /label=LuxR/HSL promoter 1890..1895 /label=-35 promoter 1912..1917 /label=-10 misc_feature 1923..1923 /label=start misc_feature 1871..1925 /label=BBa_R0062 promoter 1871..1925 /label=BBa_R0062 RBS 1934..1946 /label=RBS-3\Weak RBS 1940..1943 /label=RBS misc_feature 1934..1946 /label=BBa_B0032 RBS 1934..1946 /label=BBa_B0032 CDS 1953..2246 /label=BBa_K116602 stem_loop 2266..2309 /label=stem_loop misc_feature 2255..2334 /label=BBa_B0010 terminator 2255..2334 /label=BBa_B0010 stem_loop 2350..2369 /label=T7 TE misc_feature 2376..2376 /label=stop polyA_site 2370..2383 /label=polya misc_feature 2343..2383 /label=BBa_B0012 terminator 2343..2383 /label=BBa_B0012 misc_feature 1871..2383 /label=BBa_K116637 misc_feature 864..2383 /label=BBa_K116646 ORIGIN 1 agagcctcgt tgcgtttgtt tgcacgaacc atatgtaagt atttccttta ctagagtcac 61 acaggaaagt actagatgac tataatgata aaaaaatcgg attttttggc aattccatcg 121 gaggagtata aaggtattct aagtcttcgt tatcaagtgt ttaagcaaag acttgagtgg 181 gacttagttg tagaaaataa ccttgaatca gatgagtatg ataactcaaa tgcagaatat 241 atttatgctt gtgatgatac tgaaaatgta agtggatgct ggcgtttatt acctacaaca 301 ggtgattata tgctgaaaag tgtttttcct gaattgcttg gtcaacagag tgctcccaaa 361 gatcctaata tagtcgaatt aagtcgtttt gctgtaggta aaaatagctc aaagataaat 421 aactctgcta gtgaaattac aatgaaacta tttgaagcta tatataaaca cgctgttagt 481 caaggtatta cagaatatgt aacagtaaca tcaacagcaa tagagcgatt tttaaagcgt 541 attaaagttc cttgtcatcg tattggagac aaagaaattc atgtattagg tgatactaaa 601 tcggttgtat tgtctatgcc tattaatgaa cagtttaaaa aagcagtctt aaatgctgca 661 aacgacgaaa actacgcttt agtagcttaa taactctgat agtgctagtg tagatctcta 721 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 781 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 841 ctttctgcgt ttatatacta gagtccctat cagtgataga gattgacatc cctatcagtg 901 atagagatac tgagcactac tagagtcaca caggaaagta ctagatgaaa aacataaatg 961 ccgacgacac atacagaata attaataaaa ttaaagcttg tagaagcaat aatgatatta 1021 atcaatgctt atctgatatg actaaaatgg tacattgtga atattattta ctcgcgatca 1081 tttatcctca ttctatggtt aaatctgata tttcaatcct agataattac cctaaaaaat 1141 ggaggcaata ttatgatgac gctaatttaa taaaatatga tcctatagta gattattcta 1201 actccaatca ttcaccaatt aattggaata tatttgaaaa caatgctgta aataaaaaat 1261 ctccaaatgt aattaaagaa gcgaaaacat caggtcttat cactgggttt agtttcccta 1321 ttcatacggc taacaatggc ttcggaatgc ttagttttgc acattcagaa aaagacaact 1381 atatagatag tttattttta catgcgtgta tgaacatacc attaattgtt ccttctctag 1441 ttgataatta tcgaaaaata aatatagcaa ataataaatc aaacaacgat ttaaccaaaa 1501 gagaaaaaga atgtttagcg tgggcatgcg aaggaaaaag ctcttgggat atttcaaaaa 1561 tattaggttg cagtgagcgt actgtcactt tccatttaac caatgcgcaa atgaaactca 1621 atacaacaaa ccgctgccaa agtatttcta aagcaatttt aacaggagca attgattgcc 1681 catactttaa aaattaataa cactgatagt gctagtgtag atcactacta gagccaggca 1741 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 1801 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 1861 tatactagag acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga 1921 ataaatacta gagtcacaca ggaaagtact agatggttcg tgcaaacaaa cgcaacgagg 1981 ctctacgaat cgagagtgcg ttgcttaaca aaatcgcaat gcttggaact gagaagacag 2041 cggaagctgt gggcgttgat aagtcgcaga tcagcaggtg gaagagggac tggattccaa 2101 agttctcaat gctgcttgct gttcttgaat ggggggtcgt tgacgacgac atggctcgat 2161 tggcgcgaca agttgctgcg attctcacca ataaaaaacg cccggcggca accgagcgtt 2221 ctgaacaaat ccagatggag ttctgatact agagccaggc atcaaataaa acgaaaggct 2281 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 2341 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //