##gff-version 3
##sequence-region BBa_K116656 1 2383
BBa_K116656	.	engineered_region	1	855	.	+	0	ID=BBa_K116635;Name=BBa_K116635
BBa_K116656	.	engineered_region	57	855	.	+	0	ID=BBa_K116625;Name=BBa_K116625;Parent=BBa_K116635
BBa_K116656	.	engineered_region	76	855	.	+	0	ID=BBa_K116615;Name=BBa_K116615;Parent=BBa_K116625
BBa_K116656	.	CDS	76	693	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K116615
BBa_K116656	.	tag	655	686	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K116656	.	engineered_tag	694	718	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K116656	.	engineered_region	76	693	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K116656	.	CDS	76	654	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K116656	.	terminator	727	855	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K116615
BBa_K116656	.	terminator	815	855	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K116656	.	stem_loop	822	841	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K116656	.	engineered_region	815	855	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K116656	.	stop_codon	848	848	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K116656	.	polyA_site	842	855	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K116656	.	terminator	727	806	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K116656	.	engineered_region	727	806	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K116656	.	stem_loop	738	781	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K116656	.	ribosome_entry_site	57	69	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116625
BBa_K116656	.	ribosome_entry_site	57	69	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K116656	.	ribosome_entry_site	63	66	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K116656	.	engineered_region	57	69	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K116656	.	promoter	1	48	.	+	0	ID=BBa_K116603;Name=BBa_K116603;Parent=BBa_K116635
BBa_K116656	.	engineered_region	864	2383	.	+	0	ID=BBa_K116646;Name=BBa_K116646
BBa_K116656	.	engineered_region	1871	2383	.	+	0	ID=BBa_K116637;Name=BBa_K116637;Parent=BBa_K116646
BBa_K116656	.	terminator	2255	2334	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K116637
BBa_K116656	.	engineered_region	2255	2334	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K116656	.	stem_loop	2266	2309	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K116656	.	terminator	2343	2383	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K116637
BBa_K116656	.	stem_loop	2350	2369	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K116656	.	engineered_region	2343	2383	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K116656	.	stop_codon	2376	2376	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K116656	.	polyA_site	2370	2383	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K116656	.	ribosome_entry_site	1934	1946	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116637
BBa_K116656	.	ribosome_entry_site	1934	1946	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K116656	.	ribosome_entry_site	1940	1943	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K116656	.	engineered_region	1934	1946	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K116656	.	CDS	1953	2246	.	+	0	ID=BBa_K116602;Name=BBa_K116602;Parent=BBa_K116637
BBa_K116656	.	promoter	1871	1925	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_K116637
BBa_K116656	.	non_covalent_binding_site	1871	1890	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K116656	.	promoter	1890	1895	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K116656	.	engineered_region	1871	1925	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K116656	.	promoter	1912	1917	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K116656	.	start_codon	1923	1923	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K116656	.	engineered_region	864	1862	.	+	0	ID=BBa_K116640;Name=BBa_K116640;Parent=BBa_K116646
BBa_K116656	.	promoter	864	917	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K116640
BBa_K116656	.	promoter	883	888	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K116656	.	non_covalent_binding_site	864	882	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K116656	.	engineered_region	864	917	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K116656	.	promoter	906	911	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K116656	.	non_covalent_binding_site	889	907	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K116656	.	engineered_region	926	1862	.	+	0	ID=BBa_K116626;Name=BBa_K116626;Parent=BBa_K116640
BBa_K116656	.	engineered_region	926	1725	.	+	0	ID=BBa_K116616;Name=BBa_K116616;Parent=BBa_K116626
BBa_K116656	.	CDS	945	1700	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K116616
BBa_K116656	.	engineered_tag	1701	1725	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K116656	.	engineered_region	945	946	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K116656	.	CDS	945	1694	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K116656	.	point_mutation	1118	1118	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K116656	.	engineered_region	945	1700	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K116656	.	point_mutation	1436	1436	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K116656	.	ribosome_entry_site	926	938	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116616
BBa_K116656	.	ribosome_entry_site	926	938	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K116656	.	ribosome_entry_site	932	935	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K116656	.	engineered_region	926	938	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K116656	.	terminator	1734	1862	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K116626
BBa_K116656	.	terminator	1822	1862	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K116656	.	stem_loop	1829	1848	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K116656	.	engineered_region	1822	1862	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K116656	.	stop_codon	1855	1855	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K116656	.	polyA_site	1849	1862	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K116656	.	terminator	1734	1813	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K116656	.	engineered_region	1734	1813	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K116656	.	stem_loop	1745	1788	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K116656
agagcctcgttgcgtttgtttgcacgaaccatatgtaagtatttcctttactagagtcacacaggaaagtactagatga
ctataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagt
gtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaa
tatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctga
aaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgt
aggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgct
gttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttcctt
gtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaaca
gtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgt
agatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcccta
tcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtactagatga
aaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatg
cttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatct
gatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatccta
tagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctcc
aaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttc
ggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccat
taattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaag
agaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgt
actgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattt
taacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgc
aagaaaatggtttgttatagtcgaataaatactagagtcacacaggaaagtactagatggttcgtgcaaacaaacgcaa
cgaggctctacgaatcgagagtgcgttgcttaacaaaatcgcaatgcttggaactgagaagacagcggaagctgtgggc
gttgataagtcgcagatcagcaggtggaagagggactggattccaaagttctcaatgctgcttgctgttcttgaatggg
gggtcgttgacgacgacatggctcgattggcgcgacaagttgctgcgattctcaccaataaaaaacgcccggcggcaac
cgagcgttctgaacaaatccagatggagttctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttata