LOCUS BBa_K116658 2961 bp DNA linear 17-Dec-2024 DEFINITION pLux tester ACCESSION BBa_K116658 VERSION BBa_K116658.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 RBS 63..75 /label=RBS-3\Weak RBS 69..72 /label=RBS misc_feature 63..75 /label=BBa_B0032 RBS 63..75 /label=BBa_B0032 misc_feature 82..83 /label=prefix misc_feature 255..255 /label=T misc_feature 573..573 /label=A CDS 82..831 /label=luxR misc_feature 82..837 /label=BBa_C0062 misc_feature 838..862 /label=Help:Barcodes CDS 82..837 /label=BBa_C0062 misc_feature 63..862 /label=BBa_K116616 stem_loop 882..925 /label=stem_loop misc_feature 871..950 /label=BBa_B0010 terminator 871..950 /label=BBa_B0010 stem_loop 966..985 /label=T7 TE misc_feature 992..992 /label=stop polyA_site 986..999 /label=polya misc_feature 959..999 /label=BBa_B0012 terminator 959..999 /label=BBa_B0012 terminator 871..999 /label=BBa_B0015 misc_feature 63..999 /label=BBa_K116626 misc_feature 1..999 /label=BBa_K116640 CDS 1008..1095 /label=end of LacI coding region (inactive) misc_feature 1008..1207 /label=BBa_R0010 misc_feature 1096..1133 /label=CAP binding site promoter 1144..1149 /label=-35 promoter 1168..1173 /label=-10 misc_feature 1173..1207 /label=LacI binding site misc_feature 1180..1180 /label=start promoter 1008..1207 /label=BBa_R0010 RBS 1216..1228 /label=RBS-3\Weak RBS 1222..1225 /label=RBS misc_feature 1216..1228 /label=BBa_B0032 RBS 1216..1228 /label=BBa_B0032 misc_feature 1814..1845 /label=LVA CDS 1235..1813 /label=luxI misc_feature 1235..1852 /label=BBa_C0061 misc_feature 1853..1877 /label=Help:Barcodes CDS 1235..1852 /label=BBa_C0061 stem_loop 1897..1940 /label=stem_loop misc_feature 1886..1965 /label=BBa_B0010 terminator 1886..1965 /label=BBa_B0010 stem_loop 1981..2000 /label=T7 TE misc_feature 2007..2007 /label=stop polyA_site 2001..2014 /label=polya misc_feature 1974..2014 /label=BBa_B0012 terminator 1974..2014 /label=BBa_B0012 terminator 1886..2014 /label=BBa_B0015 misc_feature 1235..2014 /label=BBa_K116615 misc_feature 1216..2014 /label=BBa_K116625 misc_feature 1008..2014 /label=BBa_K116638 misc_feature 1..2014 /label=BBa_K116648 misc_feature 2023..2042 /label=LuxR/HSL promoter 2042..2047 /label=-35 promoter 2064..2069 /label=-10 misc_feature 2075..2075 /label=start misc_feature 2023..2077 /label=BBa_R0062 promoter 2023..2077 /label=BBa_R0062 RBS 2086..2098 /label=RBS-3\Weak RBS 2092..2095 /label=RBS misc_feature 2086..2098 /label=BBa_B0032 RBS 2086..2098 /label=BBa_B0032 CDS 2105..2824 /label=GFP protein CDS 2105..2824 /label=BBa_E0040 stem_loop 2844..2887 /label=stem_loop misc_feature 2833..2912 /label=BBa_B0010 terminator 2833..2912 /label=BBa_B0010 stem_loop 2928..2947 /label=T7 TE misc_feature 2954..2954 /label=stop polyA_site 2948..2961 /label=polya misc_feature 2921..2961 /label=BBa_B0012 terminator 2921..2961 /label=BBa_B0012 misc_feature 2023..2961 /label=BBa_K116617 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agtcacacag gaaagtacta gatgaaaaac ataaatgccg acgacacata cagaataatt 121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt 901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 961 acactggctc accttcgggt gggcctttct gcgtttatat actagagcaa tacgcaaacc 1021 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg 1081 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca 1141 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt 1201 tcacacatac tagagtcaca caggaaagta ctagatgact ataatgataa aaaaatcgga 1261 ttttttggca attccatcgg aggagtataa aggtattcta agtcttcgtt atcaagtgtt 1321 taagcaaaga cttgagtggg acttagttgt agaaaataac cttgaatcag atgagtatga 1381 taactcaaat gcagaatata tttatgcttg tgatgatact gaaaatgtaa gtggatgctg 1441 gcgtttatta cctacaacag gtgattatat gctgaaaagt gtttttcctg aattgcttgg 1501 tcaacagagt gctcccaaag atcctaatat agtcgaatta agtcgttttg ctgtaggtaa 1561 aaatagctca aagataaata actctgctag tgaaattaca atgaaactat ttgaagctat 1621 atataaacac gctgttagtc aaggtattac agaatatgta acagtaacat caacagcaat 1681 agagcgattt ttaaagcgta ttaaagttcc ttgtcatcgt attggagaca aagaaattca 1741 tgtattaggt gatactaaat cggttgtatt gtctatgcct attaatgaac agtttaaaaa 1801 agcagtctta aatgctgcaa acgacgaaaa ctacgcttta gtagcttaat aactctgata 1861 gtgctagtgt agatctctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 1921 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 1981 ggctcacctt cgggtgggcc tttctgcgtt tatatactag agacctgtag gatcgtacag 2041 gtttacgcaa gaaaatggtt tgttatagtc gaataaatac tagagtcaca caggaaagta 2101 ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga 2161 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata 2221 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac 2281 acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa 2341 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt 2401 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct 2461 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca 2521 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa 2581 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc 2641 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca 2701 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt 2761 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata 2821 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 2881 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 2941 gtgggccttt ctgcgtttat a //