##gff-version 3
##sequence-region BBa_K116658 1 2961
BBa_K116658	.	engineered_region	1	2014	.	+	0	ID=BBa_K116648;Name=BBa_K116648
BBa_K116658	.	engineered_region	1	999	.	+	0	ID=BBa_K116640;Name=BBa_K116640;Parent=BBa_K116648
BBa_K116658	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K116640
BBa_K116658	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K116658	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K116658	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K116658	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K116658	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K116658	.	engineered_region	63	999	.	+	0	ID=BBa_K116626;Name=BBa_K116626;Parent=BBa_K116640
BBa_K116658	.	engineered_region	63	862	.	+	0	ID=BBa_K116616;Name=BBa_K116616;Parent=BBa_K116626
BBa_K116658	.	CDS	82	837	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K116616
BBa_K116658	.	engineered_tag	838	862	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K116658	.	engineered_region	82	83	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K116658	.	CDS	82	831	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K116658	.	point_mutation	255	255	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K116658	.	engineered_region	82	837	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K116658	.	point_mutation	573	573	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K116658	.	ribosome_entry_site	63	75	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116616
BBa_K116658	.	ribosome_entry_site	63	75	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K116658	.	ribosome_entry_site	69	72	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K116658	.	engineered_region	63	75	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K116658	.	terminator	871	999	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K116626
BBa_K116658	.	terminator	959	999	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K116658	.	stem_loop	966	985	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K116658	.	engineered_region	959	999	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K116658	.	stop_codon	992	992	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K116658	.	polyA_site	986	999	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K116658	.	terminator	871	950	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K116658	.	engineered_region	871	950	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K116658	.	stem_loop	882	925	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K116658	.	engineered_region	1008	2014	.	+	0	ID=BBa_K116638;Name=BBa_K116638;Parent=BBa_K116648
BBa_K116658	.	promoter	1008	1207	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K116638
BBa_K116658	.	engineered_region	1008	1207	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K116658	.	non_covalent_binding_site	1173	1207	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K116658	.	CDS	1008	1095	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K116658	.	non_covalent_binding_site	1096	1133	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K116658	.	promoter	1144	1149	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K116658	.	start_codon	1180	1180	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K116658	.	promoter	1168	1173	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K116658	.	engineered_region	1216	2014	.	+	0	ID=BBa_K116625;Name=BBa_K116625;Parent=BBa_K116638
BBa_K116658	.	engineered_region	1235	2014	.	+	0	ID=BBa_K116615;Name=BBa_K116615;Parent=BBa_K116625
BBa_K116658	.	CDS	1235	1852	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K116615
BBa_K116658	.	tag	1814	1845	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K116658	.	engineered_tag	1853	1877	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K116658	.	engineered_region	1235	1852	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K116658	.	CDS	1235	1813	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K116658	.	terminator	1886	2014	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K116615
BBa_K116658	.	terminator	1974	2014	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K116658	.	stem_loop	1981	2000	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K116658	.	engineered_region	1974	2014	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K116658	.	stop_codon	2007	2007	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K116658	.	polyA_site	2001	2014	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K116658	.	terminator	1886	1965	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K116658	.	engineered_region	1886	1965	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K116658	.	stem_loop	1897	1940	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K116658	.	ribosome_entry_site	1216	1228	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116625
BBa_K116658	.	ribosome_entry_site	1216	1228	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K116658	.	ribosome_entry_site	1222	1225	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K116658	.	engineered_region	1216	1228	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K116658	.	engineered_region	2023	2961	.	+	0	ID=BBa_K116617;Name=BBa_K116617
BBa_K116658	.	CDS	2105	2824	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K116617
BBa_K116658	.	CDS	2105	2824	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K116658	.	terminator	2833	2912	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K116617
BBa_K116658	.	engineered_region	2833	2912	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K116658	.	stem_loop	2844	2887	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K116658	.	terminator	2921	2961	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K116617
BBa_K116658	.	stem_loop	2928	2947	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K116658	.	engineered_region	2921	2961	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K116658	.	stop_codon	2954	2954	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K116658	.	polyA_site	2948	2961	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K116658	.	promoter	2023	2077	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_K116617
BBa_K116658	.	non_covalent_binding_site	2023	2042	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K116658	.	promoter	2042	2047	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K116658	.	engineered_region	2023	2077	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K116658	.	promoter	2064	2069	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K116658	.	start_codon	2075	2075	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K116658	.	ribosome_entry_site	2086	2098	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116617
BBa_K116658	.	ribosome_entry_site	2086	2098	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K116658	.	ribosome_entry_site	2092	2095	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K116658	.	engineered_region	2086	2098	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
>BBa_K116658
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtact
agatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaa
tcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtt
aaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatg
atcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaa
atctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaat
ggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaaca
taccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaac
caaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagt
gagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaag
caattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctc
cccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa
ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgt
gagcggataacaatttcacacatactagagtcacacaggaaagtactagatgactataatgataaaaaaatcggatttt
ttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggact
tagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactga
aaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggt
caacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaata
actctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgt
aacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaatt
catgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctg
caaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaa
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtc
acactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaa
atggtttgttatagtcgaataaatactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactgg
agttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgat
gcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcacta
ctttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcc
cgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaa
ggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattgg
aatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattag
acacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctt
ttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttc
ttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttata