BBa_K116659
type: ComponentDefinition
persistent identity: BBa K116659
display id: BBa_K116659
version: 1
was generated by: iGEM to SBOL conversion
description: Oscillator + Tuner
created: 2008-10-30T12:00:00Z
modified: 2015-05-08T01:09:34Z
mutable provenance: ligation
owned by: james
owned by: myers
mutable description: Oscillator + Tuner. A tunable oscillator
discontinued: false
dominant: false
group u list: _210_
m user id: 0
owner id: 2749
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Jesse Wu
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_K116602
component: BBa_R0010
component: BBa_B0012
component: BBa_K116609
component: BBa_B0032
component: BBa_K116603
component: BBa_K116601
sequence annotation: BBa_K116603
sequence annotation: BBa_B0032
sequence annotation: BBa_K116601
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_K116602
sequence annotation: BBa_R0010
sequence annotation: BBa_K116609
sequence: https://synbiohub.org/public/igem/BBa_K116659_sequence/1

BBa_B0010
type: Component
persistent identity: component1998365
display id: component1998365
version: 1
top level: BBa_K116659
definition: BBa_B0010
access: public

BBa_K116602
type: Component
persistent identity: component1998364
display id: component1998364
version: 1
top level: BBa_K116659
definition: CII
access: public

BBa_R0010
type: Component
persistent identity: component1998371
display id: component1998371
version: 1
top level: BBa_K116659
definition: LacI
access: public

BBa_B0012
type: Component
persistent identity: component1998367
display id: component1998367
version: 1
top level: BBa_K116659
definition: BBa_B0012
access: public

BBa_K116609
type: Component
persistent identity: component1998381
display id: component1998381
version: 1
top level: BBa_K116659
definition: CIIICd
access: public

BBa_B0032
type: Component
persistent identity: component1998379
display id: component1998379
version: 1
top level: BBa_K116659
definition: BBa_B0032
access: public

BBa_B0012
type: Component
persistent identity: component1998384
display id: component1998384
version: 1
top level: BBa_K116659
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1998382
display id: component1998382
version: 1
top level: BBa_K116659
definition: BBa_B0010
access: public

BBa_K116603
type: Component
persistent identity: component1998349
display id: component1998349
version: 1
top level: BBa_K116659
definition: pRE
access: public

BBa_K116601
type: Component
persistent identity: component1998353
display id: component1998353
version: 1
top level: BBa_K116659
definition: BBa_K116601
access: public

BBa_B0032
type: Component
persistent identity: component1998351
display id: component1998351
version: 1
top level: BBa_K116659
definition: BBa_B0032
access: public

BBa_B0012
type: Component
persistent identity: component1998356
display id: component1998356
version: 1
top level: BBa_K116659
definition: BBa_B0012
access: public

BBa_B0032
type: Component
persistent identity: component1998362
display id: component1998362
version: 1
top level: BBa_K116659
definition: BBa_B0032
access: public

BBa_B0010
type: Component
persistent identity: component1998354
display id: component1998354
version: 1
top level: BBa_K116659
definition: BBa_B0010
access: public

BBa_K116603
type: Component
persistent identity: component1998360
display id: component1998360
version: 1
top level: BBa_K116659
definition: pRE
access: public

BBa_K116603
type: SequenceAnnotation
persistent identity: annotation1998349
display id: annotation1998349
version: 1
top level: BBa_K116659
location: https://synbiohub.org/public/igem/BBa_K116659/annotation1998349/range1998349/1
component: BBa_K116603

https://synbiohub.org/public/igem/BBa_K116659/annotation1998349/range1998349/1
type: Range
persistent identity: range1998349
display id: range1998349
version: 1
top level: BBa_K116659
direction: direction/reverse
start: 1
end: 48
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1998351
display id: annotation1998351
version: 1
top level: BBa_K116659
location: https://synbiohub.org/public/igem/BBa_K116659/annotation1998351/range1998351/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K116659/annotation1998351/range1998351/1
type: Range
persistent identity: range1998351
display id: range1998351
version: 1
top level: BBa_K116659
direction: direction/forward
start: 57
end: 69
orientation: inline

BBa_K116601
type: SequenceAnnotation
persistent identity: annotation1998353
display id: annotation1998353
version: 1
top level: BBa_K116659
location: https://synbiohub.org/public/igem/BBa_K116659/annotation1998353/range1998353/1
component: BBa_K116601

https://synbiohub.org/public/igem/BBa_K116659/annotation1998353/range1998353/1
type: Range
persistent identity: range1998353
display id: range1998353
version: 1
top level: BBa_K116659
direction: direction/reverse
start: 76
end: 2010
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1998354
display id: annotation1998354
version: 1
top level: BBa_K116659
location: https://synbiohub.org/public/igem/BBa_K116659/annotation1998354/range1998354/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K116659/annotation1998354/range1998354/1
type: Range
persistent identity: range1998354
display id: range1998354
version: 1
top level: BBa_K116659
direction: direction/forward
start: 2019
end: 2098
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1998356
display id: annotation1998356
version: 1
top level: BBa_K116659
location: https://synbiohub.org/public/igem/BBa_K116659/annotation1998356/range1998356/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K116659/annotation1998356/range1998356/1
type: Range
persistent identity: range1998356
display id: range1998356
version: 1
top level: BBa_K116659
direction: direction/forward
start: 2107
end: 2147
orientation: inline

BBa_K116603
type: SequenceAnnotation
persistent identity: annotation1998360
display id: annotation1998360
version: 1
top level: BBa_K116659
location: https://synbiohub.org/public/igem/BBa_K116659/annotation1998360/range1998360/1
component: BBa_K116603

https://synbiohub.org/public/igem/BBa_K116659/annotation1998360/range1998360/1
type: Range
persistent identity: range1998360
display id: range1998360
version: 1
top level: BBa_K116659
direction: direction/reverse
start: 2156
end: 2203
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1998362
display id: annotation1998362
version: 1
top level: BBa_K116659
location: https://synbiohub.org/public/igem/BBa_K116659/annotation1998362/range1998362/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K116659/annotation1998362/range1998362/1
type: Range
persistent identity: range1998362
display id: range1998362
version: 1
top level: BBa_K116659
direction: direction/forward
start: 2212
end: 2224
orientation: inline

BBa_K116602
type: SequenceAnnotation
persistent identity: annotation1998364
display id: annotation1998364
version: 1
top level: BBa_K116659
location: https://synbiohub.org/public/igem/BBa_K116659/annotation1998364/range1998364/1
component: BBa_K116602

https://synbiohub.org/public/igem/BBa_K116659/annotation1998364/range1998364/1
type: Range
persistent identity: range1998364
display id: range1998364
version: 1
top level: BBa_K116659
direction: direction/reverse
start: 2231
end: 2524
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1998365
display id: annotation1998365
version: 1
top level: BBa_K116659
location: https://synbiohub.org/public/igem/BBa_K116659/annotation1998365/range1998365/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K116659/annotation1998365/range1998365/1
type: Range
persistent identity: range1998365
display id: range1998365
version: 1
top level: BBa_K116659
direction: direction/forward
start: 2533
end: 2612
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1998367
display id: annotation1998367
version: 1
top level: BBa_K116659
location: https://synbiohub.org/public/igem/BBa_K116659/annotation1998367/range1998367/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K116659/annotation1998367/range1998367/1
type: Range
persistent identity: range1998367
display id: range1998367
version: 1
top level: BBa_K116659
direction: direction/forward
start: 2621
end: 2661
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1998371
display id: annotation1998371
version: 1
top level: BBa_K116659
location: https://synbiohub.org/public/igem/BBa_K116659/annotation1998371/range1998371/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K116659/annotation1998371/range1998371/1
type: Range
persistent identity: range1998371
display id: range1998371
version: 1
top level: BBa_K116659
direction: direction/forward
start: 2670
end: 2869
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1998379
display id: annotation1998379
version: 1
top level: BBa_K116659
location: https://synbiohub.org/public/igem/BBa_K116659/annotation1998379/range1998379/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K116659/annotation1998379/range1998379/1
type: Range
persistent identity: range1998379
display id: range1998379
version: 1
top level: BBa_K116659
direction: direction/forward
start: 2878
end: 2890
orientation: inline

BBa_K116609
type: SequenceAnnotation
persistent identity: annotation1998381
display id: annotation1998381
version: 1
top level: BBa_K116659
location: https://synbiohub.org/public/igem/BBa_K116659/annotation1998381/range1998381/1
component: BBa_K116609

https://synbiohub.org/public/igem/BBa_K116659/annotation1998381/range1998381/1
type: Range
persistent identity: range1998381
display id: range1998381
version: 1
top level: BBa_K116659
direction: direction/reverse
start: 2897
end: 2986
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1998382
display id: annotation1998382
version: 1
top level: BBa_K116659
location: https://synbiohub.org/public/igem/BBa_K116659/annotation1998382/range1998382/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K116659/annotation1998382/range1998382/1
type: Range
persistent identity: range1998382
display id: range1998382
version: 1
top level: BBa_K116659
direction: direction/forward
start: 2995
end: 3074
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1998384
display id: annotation1998384
version: 1
top level: BBa_K116659
location: https://synbiohub.org/public/igem/BBa_K116659/annotation1998384/range1998384/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K116659/annotation1998384/range1998384/1
type: Range
persistent identity: range1998384
display id: range1998384
version: 1
top level: BBa_K116659
direction: direction/forward
start: 3083
end: 3123
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_K116601
type: ComponentDefinition
persistent identity: BBa K116601
display id: BBa_K116601
version: 1
was generated by: iGEM to SBOL conversion
description: HtlB (ftsH) coding region from E. coli
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:33Z
mutable provenance: E. coli K. 12 MG1655
owned by: james
owned by: myers
mutable description: The HtlB (ftsH) coding region from E. coli. It can be used to degrade many different proteins.
discontinued: false
dominant: false
group u list: _210_
m user id: 0
owner id: 2749
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: PCR primer design
star: false
creator: Jesse Wu
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K116601_sequence/1

pRE
type: ComponentDefinition
persistent identity: BBa K116603
display id: BBa_K116603
version: 1
was generated by: iGEM to SBOL conversion
description: pRE promoter from λ phage
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:33Z
mutable provenance: λ phage
owned by: james
owned by: myers
mutable description: The pRE coding region from λ phage. It is able to be induced by CII (BBa_K116602).
discontinued: false
dominant: false
experience: experience/None
group u list: _210_
m user id: 0
owner id: 2749
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none.
star: false
creator: Jesse Wu
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K116603_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

CII
type: ComponentDefinition
persistent identity: BBa K116602
display id: BBa_K116602
version: 1
was generated by: iGEM to SBOL conversion
description: CII coding region from λ phage
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:33Z
mutable provenance: λ phage
owned by: james
owned by: myers
mutable description: The CII coding region from λ phage.
discontinued: false
dominant: true
experience: experience/None
group u list: _210_
m user id: 0
owner id: 2749
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none.
star: false
creator: Jesse Wu
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K116602_sequence/1

CIIICd
type: ComponentDefinition
persistent identity: BBa K116609
display id: BBa_K116609
version: 1
was generated by: iGEM to SBOL conversion
description: CIIICd: modified CIII coding region from λ phage
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:33Z
mutable provenance: λ phage
owned by: james
owned by: myers
mutable description: CIIICd: A modified version of the CIII coding region from ''λ phage''. '''Original CIII''': '''ATG'''CAATATGCCATTGCAGGGTGGCCTGTTGCTGGCTGC'''CCTTCCGAATCTTTACTTGAACGAATCACCC''' '''GTAAATTACGTGACGGATGGAAACGCCTTATCGACATACTTAATCAGCCAGGAG'''TCCCAAAGAATGGATC AAACACTTATGGCTATCCAGAC'''TAA''' The protein domains from the left-most and right-most side of CIII have been removed (the grayed out parts), i.e. amino acid residues #2-13 and #42-49 have been excluded, leaving residues #1 (start codon), #14-41 (the protein domain in the middle) and #55 (stop codon).
discontinued: false
dominant: false
experience: experience/None
group u list: _210_
m user id: 0
owner id: 2749
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: HtlB also degrades CIII, creating competitive degradation reactions. We removed the leftmost and right most protein domains since Hadler ''et al'' said that removing these two protein domains will stop HtlB from degrading CIII. Doing this makes it easier to predict and model.
star: false
creator: Jesse Wu
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K116609_sequence/1

https://synbiohub.org/public/igem/BBa_K116659_sequence/1
type: Sequence
persistent identity: BBa K116659 sequence
display id: BBa_K116659_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagcctcgttgcgtttgtttgcacgaaccatatgtaagtatttcctttactagagtcacacaggaaagtactagatggcgaaaaacctaatactctggctggtcattgccgttgtgctgatgtcagtattccagagctttgggcccagcgagtctaatggccgtaaggtggattactctaccttcctacaagaggtcaataacgaccaggttcgtgaagcgcgtatcaacggacgtgaaatcaacgttaccaagaaagatagtaaccgttataccacttacattccggttcaggatccgaaattactggataacctgttgaccaagaacgtcaaggttgtcggtgaaccgcctgaagaaccaagcctgctggcttctatcttcatctcctggttcccgatgctgttgctgattggtgtctggatcttcttcatgcgtcaaatgcagggcggcggtggcaaaggtgccatgtcgtttggtaagagcaaagcgcgcatgctgacggaagatcagatcaaaacgacctttgctgacgttgcgggctgcgacgaagcaaaagaagaagttgctgaactggttgagtatctgcgcgagccgagccgcttccagaaactcggcggtaagatcccgaaaggcgtcttgatggtcggtcctccgggtaccggtaaaacgctgctggcgaaagcgattgcaggcgaagcgaaagttccgttctttactatctccggttctgacttcgtagaaatgttcgtcggtgtgggtgcatcccgtgttcgtgacatgttcgaacaggcgaagaaagcggcaccgtgcatcatctttatcgatgaaatcgacgccgtaggccgccagcgtggcgctggtctgggcggtggtcacgatgaacgtgaacagactctgaaccagatgctggttgagatggatggcttcgaaggtaacgaaggtatcatcgttatcgccgcgactaaccgtccggacgttctcgacccggccctgctgcgtcctggccgtttcgaccgtcaggttgtggtcggcttgccagatgttcgcggtcgtgagcagatcctgaaagttcacatgcgtcgcgtaccattggcacccgatatcgacgcggcaatcattgcccgtggtactcctggtttctccggtgctgacctggcgaacctggtgaacgaagcggcactgttcgctgctcgtggcaacaaacgcgttgtgtcgatggttgagttcgagaaagcgaaagacaaaatcatgatgggtgcggaacgtcgctccatggtgatgacggaagcgcagaaagaatcgacggcttaccacgaagcgggtcatgcgattatcggtcgcctggtgccggaacacgatccggtgcacaaagtgacgattatcccacgcggtcgtgcgctgggtgtgactttcttcttgcctgagggcgacgcaatcagcgccagccgtcagaaactggaaagccagatttctacgctgtacggtggtcgtctggcagaagagatcatctacgggccggaacatgtatctaccggtgcgtccaacgatattaaagttgcgaccaacctggcacgtaacatggtgactcagtggggcttctctgagaaattgggtccactgctgtacgcggaagaagaaggtgaagtgttcctcggccgtagcgtagcgaaagcgaaacatatgtccgatgaaactgcacgtatcatcgaccaggaagtgaaagcactgattgagcgtaactataatcgtgcgcgtcagcttctgaccgacaatatggatattctgcatgcgatgaaagatgctctcatgaaatatgagactatcgacgcaccgcagattgatgacctgatggcacgtcgcgatgtacgtccgccagcgggctgggaagaaccaggcgcttctaacaattctggcgacaatggtagtccaaaggctcctcgtccggttgatgaaccgcgtacgccgaacccgggtaacaccatgtcagagcagttagg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K116601_sequence/1
type: Sequence
persistent identity: BBa K116601 sequence
display id: BBa_K116601_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcgaaaaacctaatactctggctggtcattgccgttgtgctgatgtcagtattccagagctttgggcccagcgagtctaatggccgtaaggtggattactctaccttcctacaagaggtcaataacgaccaggttcgtgaagcgcgtatcaacggacgtgaaatcaacgttaccaagaaagatagtaaccgttataccacttacattccggttcaggatccgaaattactggataacctgttgaccaagaacgtcaaggttgtcggtgaaccgcctgaagaaccaagcctgctggcttctatcttcatctcctggttcccgatgctgttgctgattggtgtctggatcttcttcatgcgtcaaatgcagggcggcggtggcaaaggtgccatgtcgtttggtaagagcaaagcgcgcatgctgacggaagatcagatcaaaacgacctttgctgacgttgcgggctgcgacgaagcaaaagaagaagttgctgaactggttgagtatctgcgcgagccgagccgcttccagaaactcggcggtaagatcccgaaaggcgtcttgatggtcggtcctccgggtaccggtaaaacgctgctggcgaaagcgattgcaggcgaagcgaaagttccgttctttactatctccggttctgacttcgtagaaatgttcgtcggtgtgggtgcatcccgtgttcgtgacatgttcgaacaggcgaagaaagcggcaccgtgcatcatctttatcgatgaaatcgacgccgtaggccgccagcgtggcgctggtctgggcggtggtcacgatgaacgtgaacagactctgaaccagatgctggttgagatggatggcttcgaaggtaacgaaggtatcatcgttatcgccgcgactaaccgtccggacgttctcgacccggccctgctgcgtcctggccgtttcgaccgtcaggttgtggtcggcttgccagatgttcgcggtcgtgagcagatcctgaaagttcacatgcgtcgcgtaccattggcacccgatatcgacgcggcaatcattgcccgtggtactcctggtttctccggtgctgacctggcgaacctggtgaacgaagcggcactgttcgctgctcgtggcaacaaacgcgttgtgtcgatggttgagttcgagaaagcgaaagacaaaatcatgatgggtgcggaacgtcgctccatggtgatgacggaagcgcagaaagaatcgacggcttaccacgaagcgggtcatgcgattatcggtcgcctggtgccggaacacgatccggtgcacaaagtgacgattatcccacgcggtcgtgcgctgggtgtgactttcttcttgcctgagggcgacgcaatcagcgccagccgtcagaaactggaaagccagatttctacgctgtacggtggtcgtctggcagaagagatcatctacgggccggaacatgtatctaccggtgcgtccaacgatattaaagttgcgaccaacctggcacgtaacatggtgactcagtggggcttctctgagaaattgggtccactgctgtacgcggaagaagaaggtgaagtgttcctcggccgtagcgtagcgaaagcgaaacatatgtccgatgaaactgcacgtatcatcgaccaggaagtgaaagcactgattgagcgtaactataatcgtgcgcgtcagcttctgaccgacaatatggatattctgcatgcgatgaaagatgctctcatgaaatatgagactatcgacgcaccgcagattgatgacctgatggcacgtcgcgatgtacgtccgccagcgggctgggaagaaccaggcgcttctaacaattctggcgacaatggtagtccaaaggctcctcgtccggttgatgaaccgcgtacgccgaacccgggtaacaccatgtcagagcagttaggcgacaagtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K116603_sequence/1
type: Sequence
persistent identity: BBa K116603 sequence
display id: BBa_K116603_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagcctcgttgcgtttgtttgcacgaaccatatgtaagtatttcctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K116602_sequence/1
type: Sequence
persistent identity: BBa K116602 sequence
display id: BBa_K116602_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggttcgtgcaaacaaacgcaacgaggctctacgaatcgagagtgcgttgcttaacaaaatcgcaatgcttggaactgagaagacagcggaagctgtgggcgttgataagtcgcagatcagcaggtggaagagggactggattccaaagttctcaatgctgcttgctgttcttgaatggggggtcgttgacgacgacatggctcgattggcgcgacaagttgctgcgattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K116609_sequence/1
type: Sequence
persistent identity: BBa K116609 sequence
display id: BBa_K116609_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgccttccgaatctttacttgaacgaatcacccgtaaattacgtgacggatggaaacgccttatcgacatacttaatcagccaggataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z