##gff-version 3
##sequence-region BBa_K116665 1 2184
BBa_K116665	.	engineered_region	1	1726	.	+	0	ID=BBa_K116655;Name=BBa_K116655
BBa_K116665	.	engineered_region	57	1726	.	+	0	ID=BBa_K116645;Name=BBa_K116645;Parent=BBa_K116655
BBa_K116665	.	engineered_region	57	1663	.	+	0	ID=BBa_K116634;Name=BBa_K116634;Parent=BBa_K116645
BBa_K116665	.	engineered_region	57	856	.	+	0	ID=BBa_K116616;Name=BBa_K116616;Parent=BBa_K116634
BBa_K116665	.	CDS	76	831	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K116616
BBa_K116665	.	engineered_tag	832	856	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K116665	.	engineered_region	76	831	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K116665	.	engineered_region	76	77	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K116665	.	point_mutation	249	249	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K116665	.	CDS	76	825	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K116665	.	point_mutation	567	567	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K116665	.	ribosome_entry_site	57	69	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116616
BBa_K116665	.	ribosome_entry_site	57	69	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K116665	.	engineered_region	57	69	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K116665	.	ribosome_entry_site	63	66	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K116665	.	engineered_region	865	1663	.	+	0	ID=BBa_K116625;Name=BBa_K116625;Parent=BBa_K116634
BBa_K116665	.	engineered_region	884	1663	.	+	0	ID=BBa_K116615;Name=BBa_K116615;Parent=BBa_K116625
BBa_K116665	.	terminator	1535	1663	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K116615
BBa_K116665	.	terminator	1535	1614	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K116665	.	engineered_region	1535	1614	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K116665	.	stem_loop	1546	1589	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K116665	.	terminator	1623	1663	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K116665	.	stop_codon	1656	1656	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K116665	.	polyA_site	1650	1663	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K116665	.	stem_loop	1630	1649	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K116665	.	engineered_region	1623	1663	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K116665	.	CDS	884	1501	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K116615
BBa_K116665	.	CDS	884	1462	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K116665	.	engineered_tag	1502	1526	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K116665	.	engineered_region	884	1501	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K116665	.	tag	1463	1494	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K116665	.	ribosome_entry_site	865	877	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116625
BBa_K116665	.	ribosome_entry_site	865	877	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K116665	.	engineered_region	865	877	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K116665	.	ribosome_entry_site	871	874	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K116665	.	promoter	1672	1726	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_K116645
BBa_K116665	.	start_codon	1724	1724	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K116665	.	promoter	1713	1718	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K116665	.	promoter	1691	1696	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K116665	.	engineered_region	1672	1726	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K116665	.	non_covalent_binding_site	1672	1691	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K116665	.	promoter	1	48	.	+	0	ID=BBa_K116603;Name=BBa_K116603;Parent=BBa_K116655
BBa_K116665	.	engineered_region	1735	2184	.	+	0	ID=BBa_K116622;Name=BBa_K116622
BBa_K116665	.	ribosome_entry_site	1735	1747	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116622
BBa_K116665	.	ribosome_entry_site	1735	1747	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K116665	.	engineered_region	1735	1747	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K116665	.	ribosome_entry_site	1741	1744	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K116665	.	CDS	1754	2047	.	+	0	ID=BBa_K116602;Name=BBa_K116602;Parent=BBa_K116622
BBa_K116665	.	terminator	2056	2135	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K116622
BBa_K116665	.	engineered_region	2056	2135	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K116665	.	stem_loop	2067	2110	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K116665	.	terminator	2144	2184	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K116622
BBa_K116665	.	stop_codon	2177	2177	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K116665	.	polyA_site	2171	2184	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K116665	.	stem_loop	2151	2170	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K116665	.	engineered_region	2144	2184	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K116665
agagcctcgttgcgtttgtttgcacgaaccatatgtaagtatttcctttactagagtcacacaggaaagtactagatga
aaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatg
cttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatct
gatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatccta
tagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctcc
aaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttc
ggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccat
taattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaag
agaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgt
actgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattt
taacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagtcaca
caggaaagtactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattct
aagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtat
gataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaa
caggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcga
attaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaa
gctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaa
agcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtc
tatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataa
ctctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt
tcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtt
tatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagtcac
acaggaaagtactagatggttcgtgcaaacaaacgcaacgaggctctacgaatcgagagtgcgttgcttaacaaaatcg
caatgcttggaactgagaagacagcggaagctgtgggcgttgataagtcgcagatcagcaggtggaagagggactggat
tccaaagttctcaatgctgcttgctgttcttgaatggggggtcgttgacgacgacatggctcgattggcgcgacaagtt
gctgcgattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgatactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata