LOCUS BBa_K116669 4063 bp DNA linear 22-Nov-2024 DEFINITION Oscillator + Tuner + Reporter ACCESSION BBa_K116669 VERSION BBa_K116669.1 FEATURES Location/Qualifiers promoter 1..48 /label=BBa_K116603 RBS 57..69 /label=RBS-3\Weak RBS 63..66 /label=RBS misc_feature 57..69 /label=BBa_B0032 RBS 57..69 /label=BBa_B0032 CDS 76..2010 /label=BBa_K116601 stem_loop 2030..2073 /label=stem_loop misc_feature 2019..2098 /label=BBa_B0010 terminator 2019..2098 /label=BBa_B0010 stem_loop 2114..2133 /label=T7 TE misc_feature 2140..2140 /label=stop polyA_site 2134..2147 /label=polya misc_feature 2107..2147 /label=BBa_B0012 terminator 2107..2147 /label=BBa_B0012 promoter 2156..2203 /label=BBa_K116603 RBS 2212..2224 /label=RBS-3\Weak RBS 2218..2221 /label=RBS misc_feature 2212..2224 /label=BBa_B0032 RBS 2212..2224 /label=BBa_B0032 CDS 2231..2524 /label=BBa_K116602 stem_loop 2544..2587 /label=stem_loop misc_feature 2533..2612 /label=BBa_B0010 terminator 2533..2612 /label=BBa_B0010 stem_loop 2628..2647 /label=T7 TE misc_feature 2654..2654 /label=stop polyA_site 2648..2661 /label=polya misc_feature 2621..2661 /label=BBa_B0012 terminator 2621..2661 /label=BBa_B0012 CDS 2670..2757 /label=end of LacI coding region (inactive) misc_feature 2670..2869 /label=BBa_R0010 misc_feature 2758..2795 /label=CAP binding site promoter 2806..2811 /label=-35 promoter 2830..2835 /label=-10 misc_feature 2835..2869 /label=LacI binding site misc_feature 2842..2842 /label=start promoter 2670..2869 /label=BBa_R0010 RBS 2878..2890 /label=RBS-3\Weak RBS 2884..2887 /label=RBS misc_feature 2878..2890 /label=BBa_B0032 RBS 2878..2890 /label=BBa_B0032 CDS 2897..2986 /label=BBa_K116609 stem_loop 3006..3049 /label=stem_loop misc_feature 2995..3074 /label=BBa_B0010 terminator 2995..3074 /label=BBa_B0010 stem_loop 3090..3109 /label=T7 TE misc_feature 3116..3116 /label=stop polyA_site 3110..3123 /label=polya misc_feature 3083..3123 /label=BBa_B0012 terminator 3083..3123 /label=BBa_B0012 promoter 3132..3179 /label=BBa_K116603 RBS 3188..3200 /label=RBS-3\Weak RBS 3194..3197 /label=RBS misc_feature 3188..3200 /label=BBa_B0032 RBS 3188..3200 /label=BBa_B0032 CDS 3207..3926 /label=GFP protein CDS 3207..3926 /label=BBa_E0040 stem_loop 3946..3989 /label=stem_loop misc_feature 3935..4014 /label=BBa_B0010 terminator 3935..4014 /label=BBa_B0010 stem_loop 4030..4049 /label=T7 TE misc_feature 4056..4056 /label=stop polyA_site 4050..4063 /label=polya misc_feature 4023..4063 /label=BBa_B0012 terminator 4023..4063 /label=BBa_B0012 ORIGIN 1 agagcctcgt tgcgtttgtt tgcacgaacc atatgtaagt atttccttta ctagagtcac 61 acaggaaagt actagatggc gaaaaaccta atactctggc tggtcattgc cgttgtgctg 121 atgtcagtat tccagagctt tgggcccagc gagtctaatg gccgtaaggt ggattactct 181 accttcctac aagaggtcaa taacgaccag gttcgtgaag cgcgtatcaa cggacgtgaa 241 atcaacgtta ccaagaaaga tagtaaccgt tataccactt acattccggt tcaggatccg 301 aaattactgg ataacctgtt gaccaagaac gtcaaggttg tcggtgaacc gcctgaagaa 361 ccaagcctgc tggcttctat cttcatctcc tggttcccga tgctgttgct gattggtgtc 421 tggatcttct tcatgcgtca aatgcagggc ggcggtggca aaggtgccat gtcgtttggt 481 aagagcaaag cgcgcatgct gacggaagat cagatcaaaa cgacctttgc tgacgttgcg 541 ggctgcgacg aagcaaaaga agaagttgct gaactggttg agtatctgcg cgagccgagc 601 cgcttccaga aactcggcgg taagatcccg aaaggcgtct tgatggtcgg tcctccgggt 661 accggtaaaa cgctgctggc gaaagcgatt gcaggcgaag cgaaagttcc gttctttact 721 atctccggtt ctgacttcgt agaaatgttc gtcggtgtgg gtgcatcccg tgttcgtgac 781 atgttcgaac aggcgaagaa agcggcaccg tgcatcatct ttatcgatga aatcgacgcc 841 gtaggccgcc agcgtggcgc tggtctgggc ggtggtcacg atgaacgtga acagactctg 901 aaccagatgc tggttgagat ggatggcttc gaaggtaacg aaggtatcat cgttatcgcc 961 gcgactaacc gtccggacgt tctcgacccg gccctgctgc gtcctggccg tttcgaccgt 1021 caggttgtgg tcggcttgcc agatgttcgc ggtcgtgagc agatcctgaa agttcacatg 1081 cgtcgcgtac cattggcacc cgatatcgac gcggcaatca ttgcccgtgg tactcctggt 1141 ttctccggtg ctgacctggc gaacctggtg aacgaagcgg cactgttcgc tgctcgtggc 1201 aacaaacgcg ttgtgtcgat ggttgagttc gagaaagcga aagacaaaat catgatgggt 1261 gcggaacgtc gctccatggt gatgacggaa gcgcagaaag aatcgacggc ttaccacgaa 1321 gcgggtcatg cgattatcgg tcgcctggtg ccggaacacg atccggtgca caaagtgacg 1381 attatcccac gcggtcgtgc gctgggtgtg actttcttct tgcctgaggg cgacgcaatc 1441 agcgccagcc gtcagaaact ggaaagccag atttctacgc tgtacggtgg tcgtctggca 1501 gaagagatca tctacgggcc ggaacatgta tctaccggtg cgtccaacga tattaaagtt 1561 gcgaccaacc tggcacgtaa catggtgact cagtggggct tctctgagaa attgggtcca 1621 ctgctgtacg cggaagaaga aggtgaagtg ttcctcggcc gtagcgtagc gaaagcgaaa 1681 catatgtccg atgaaactgc acgtatcatc gaccaggaag tgaaagcact gattgagcgt 1741 aactataatc gtgcgcgtca gcttctgacc gacaatatgg atattctgca tgcgatgaaa 1801 gatgctctca tgaaatatga gactatcgac gcaccgcaga ttgatgacct gatggcacgt 1861 cgcgatgtac gtccgccagc gggctgggaa gaaccaggcg cttctaacaa ttctggcgac 1921 aatggtagtc caaaggctcc tcgtccggtt gatgaaccgc gtacgccgaa cccgggtaac 1981 accatgtcag agcagttagg cgacaagtaa tactagagcc aggcatcaaa taaaacgaaa 2041 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 2101 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagagagc 2161 ctcgttgcgt ttgtttgcac gaaccatatg taagtatttc ctttactaga gtcacacagg 2221 aaagtactag atggttcgtg caaacaaacg caacgaggct ctacgaatcg agagtgcgtt 2281 gcttaacaaa atcgcaatgc ttggaactga gaagacagcg gaagctgtgg gcgttgataa 2341 gtcgcagatc agcaggtgga agagggactg gattccaaag ttctcaatgc tgcttgctgt 2401 tcttgaatgg ggggtcgttg acgacgacat ggctcgattg gcgcgacaag ttgctgcgat 2461 tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt 2521 ctgatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 2581 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 2641 gtgggccttt ctgcgtttat atactagagc aatacgcaaa ccgcctctcc ccgcgcgttg 2701 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 2761 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct 2821 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacat actagagtca 2881 cacaggaaag tactagatgc cttccgaatc tttacttgaa cgaatcaccc gtaaattacg 2941 tgacggatgg aaacgcctta tcgacatact taatcagcca ggataatact agagccaggc 3001 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 3061 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 3121 atatactaga gagagcctcg ttgcgtttgt ttgcacgaac catatgtaag tatttccttt 3181 actagagtca cacaggaaag tactagatgc gtaaaggaga agaacttttc actggagttg 3241 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag 3301 agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa 3361 aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg 3421 cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt 3481 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag 3541 tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag 3601 aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca 3661 tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg 3721 aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc 3781 ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca 3841 acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg 3901 gcatggatga actatacaaa taataatact agagccaggc atcaaataaa acgaaaggct 3961 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 4021 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //