##gff-version 3
##sequence-region BBa_K116669 1 4063
BBa_K116669	.	promoter	2670	2869	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K116669	.	engineered_region	2670	2869	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K116669	.	non_covalent_binding_site	2835	2869	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K116669	.	CDS	2670	2757	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K116669	.	non_covalent_binding_site	2758	2795	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K116669	.	promoter	2806	2811	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K116669	.	start_codon	2842	2842	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K116669	.	promoter	2830	2835	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K116669	.	CDS	76	2010	.	+	0	ID=BBa_K116601;Name=BBa_K116601
BBa_K116669	.	promoter	3132	3179	.	+	0	ID=BBa_K116603;Name=BBa_K116603
BBa_K116669	.	terminator	2533	2612	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K116669	.	engineered_region	2533	2612	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K116669	.	stem_loop	2544	2587	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K116669	.	promoter	2156	2203	.	+	0	ID=BBa_K116603;Name=BBa_K116603
BBa_K116669	.	terminator	3935	4014	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K116669	.	engineered_region	3935	4014	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K116669	.	stem_loop	3946	3989	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K116669	.	ribosome_entry_site	3188	3200	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K116669	.	ribosome_entry_site	3188	3200	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K116669	.	ribosome_entry_site	3194	3197	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K116669	.	engineered_region	3188	3200	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K116669	.	CDS	3207	3926	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K116669	.	CDS	3207	3926	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K116669	.	ribosome_entry_site	2212	2224	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K116669	.	ribosome_entry_site	2212	2224	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K116669	.	ribosome_entry_site	2218	2221	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K116669	.	engineered_region	2212	2224	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K116669	.	terminator	2107	2147	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K116669	.	stem_loop	2114	2133	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K116669	.	engineered_region	2107	2147	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K116669	.	stop_codon	2140	2140	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K116669	.	polyA_site	2134	2147	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K116669	.	terminator	3083	3123	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K116669	.	stem_loop	3090	3109	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K116669	.	engineered_region	3083	3123	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K116669	.	stop_codon	3116	3116	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K116669	.	polyA_site	3110	3123	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K116669	.	CDS	2897	2986	.	+	0	ID=BBa_K116609;Name=BBa_K116609
BBa_K116669	.	terminator	4023	4063	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K116669	.	stem_loop	4030	4049	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K116669	.	engineered_region	4023	4063	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K116669	.	stop_codon	4056	4056	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K116669	.	polyA_site	4050	4063	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K116669	.	promoter	1	48	.	+	0	ID=BBa_K116603;Name=BBa_K116603
BBa_K116669	.	CDS	2231	2524	.	+	0	ID=BBa_K116602;Name=BBa_K116602
BBa_K116669	.	terminator	2621	2661	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K116669	.	stem_loop	2628	2647	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K116669	.	engineered_region	2621	2661	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K116669	.	stop_codon	2654	2654	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K116669	.	polyA_site	2648	2661	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K116669	.	ribosome_entry_site	2878	2890	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K116669	.	ribosome_entry_site	2878	2890	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K116669	.	ribosome_entry_site	2884	2887	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K116669	.	engineered_region	2878	2890	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K116669	.	terminator	2019	2098	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K116669	.	engineered_region	2019	2098	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K116669	.	stem_loop	2030	2073	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K116669	.	terminator	2995	3074	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K116669	.	engineered_region	2995	3074	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K116669	.	stem_loop	3006	3049	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K116669	.	ribosome_entry_site	57	69	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K116669	.	ribosome_entry_site	57	69	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K116669	.	ribosome_entry_site	63	66	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K116669	.	engineered_region	57	69	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
>BBa_K116669
agagcctcgttgcgtttgtttgcacgaaccatatgtaagtatttcctttactagagtcacacaggaaagtactagatgg
cgaaaaacctaatactctggctggtcattgccgttgtgctgatgtcagtattccagagctttgggcccagcgagtctaa
tggccgtaaggtggattactctaccttcctacaagaggtcaataacgaccaggttcgtgaagcgcgtatcaacggacgt
gaaatcaacgttaccaagaaagatagtaaccgttataccacttacattccggttcaggatccgaaattactggataacc
tgttgaccaagaacgtcaaggttgtcggtgaaccgcctgaagaaccaagcctgctggcttctatcttcatctcctggtt
cccgatgctgttgctgattggtgtctggatcttcttcatgcgtcaaatgcagggcggcggtggcaaaggtgccatgtcg
tttggtaagagcaaagcgcgcatgctgacggaagatcagatcaaaacgacctttgctgacgttgcgggctgcgacgaag
caaaagaagaagttgctgaactggttgagtatctgcgcgagccgagccgcttccagaaactcggcggtaagatcccgaa
aggcgtcttgatggtcggtcctccgggtaccggtaaaacgctgctggcgaaagcgattgcaggcgaagcgaaagttccg
ttctttactatctccggttctgacttcgtagaaatgttcgtcggtgtgggtgcatcccgtgttcgtgacatgttcgaac
aggcgaagaaagcggcaccgtgcatcatctttatcgatgaaatcgacgccgtaggccgccagcgtggcgctggtctggg
cggtggtcacgatgaacgtgaacagactctgaaccagatgctggttgagatggatggcttcgaaggtaacgaaggtatc
atcgttatcgccgcgactaaccgtccggacgttctcgacccggccctgctgcgtcctggccgtttcgaccgtcaggttg
tggtcggcttgccagatgttcgcggtcgtgagcagatcctgaaagttcacatgcgtcgcgtaccattggcacccgatat
cgacgcggcaatcattgcccgtggtactcctggtttctccggtgctgacctggcgaacctggtgaacgaagcggcactg
ttcgctgctcgtggcaacaaacgcgttgtgtcgatggttgagttcgagaaagcgaaagacaaaatcatgatgggtgcgg
aacgtcgctccatggtgatgacggaagcgcagaaagaatcgacggcttaccacgaagcgggtcatgcgattatcggtcg
cctggtgccggaacacgatccggtgcacaaagtgacgattatcccacgcggtcgtgcgctgggtgtgactttcttcttg
cctgagggcgacgcaatcagcgccagccgtcagaaactggaaagccagatttctacgctgtacggtggtcgtctggcag
aagagatcatctacgggccggaacatgtatctaccggtgcgtccaacgatattaaagttgcgaccaacctggcacgtaa
catggtgactcagtggggcttctctgagaaattgggtccactgctgtacgcggaagaagaaggtgaagtgttcctcggc
cgtagcgtagcgaaagcgaaacatatgtccgatgaaactgcacgtatcatcgaccaggaagtgaaagcactgattgagc
gtaactataatcgtgcgcgtcagcttctgaccgacaatatggatattctgcatgcgatgaaagatgctctcatgaaata
tgagactatcgacgcaccgcagattgatgacctgatggcacgtcgcgatgtacgtccgccagcgggctgggaagaacca
ggcgcttctaacaattctggcgacaatggtagtccaaaggctcctcgtccggttgatgaaccgcgtacgccgaacccgg
gtaacaccatgtcagagcagttaggcgacaagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagagagcctcgttgcgtttgtttgcacgaaccatatgtaagtatttcctttactagagt
cacacaggaaagtactagatggttcgtgcaaacaaacgcaacgaggctctacgaatcgagagtgcgttgcttaacaaaa
tcgcaatgcttggaactgagaagacagcggaagctgtgggcgttgataagtcgcagatcagcaggtggaagagggactg
gattccaaagttctcaatgctgcttgctgttcttgaatggggggtcgttgacgacgacatggctcgattggcgcgacaa
gttgctgcgattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgatact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcct
ctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacg
caattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaat
tgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatgccttccgaatctttacttgaacga
atcacccgtaaattacgtgacggatggaaacgccttatcgacatacttaatcagccaggataatactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagcctcgttgcgtttgtttgcacgaac
catatgtaagtatttcctttactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagtt
gtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaa
catacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactacttt
cggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaa
ggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtg
atacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaata
caactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacac
aacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttac
cagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttga
gtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttata