BBa_K116669
type: ComponentDefinition
persistent identity: BBa K116669
display id: BBa_K116669
version: 1
was generated by: iGEM to SBOL conversion
description: Oscillator + Tuner + Reporter
created: 2008-10-30T12:00:00Z
modified: 2015-05-08T01:09:34Z
mutable provenance: ligation
owned by: james
owned by: myers
mutable description: Oscillator + Tuner + Reporter
discontinued: false
dominant: false
group u list: _210_
m user id: 0
owner id: 2749
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Jesse Wu
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0010
component: BBa_K116603
component: BBa_B0012
component: BBa_K116602
component: BBa_B0032
component: BBa_B0010
component: BBa_K116601
component: BBa_E0040
component: BBa_K116609
sequence annotation: BBa_K116603
sequence annotation: BBa_B0032
sequence annotation: BBa_K116601
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_K116602
sequence annotation: BBa_R0010
sequence annotation: BBa_K116609
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K116669_sequence/1

BBa_R0010
type: Component
persistent identity: component1998467
display id: component1998467
version: 1
top level: BBa_K116669
definition: LacI
access: public

BBa_K116603
type: Component
persistent identity: component1998456
display id: component1998456
version: 1
top level: BBa_K116669
definition: pRE
access: public

BBa_B0012
type: Component
persistent identity: component1998452
display id: component1998452
version: 1
top level: BBa_K116669
definition: BBa_B0012
access: public

BBa_K116602
type: Component
persistent identity: component1998460
display id: component1998460
version: 1
top level: BBa_K116669
definition: CII
access: public

BBa_B0032
type: Component
persistent identity: component1998458
display id: component1998458
version: 1
top level: BBa_K116669
definition: BBa_B0032
access: public

BBa_B0032
type: Component
persistent identity: component1998447
display id: component1998447
version: 1
top level: BBa_K116669
definition: BBa_B0032
access: public

BBa_K116603
type: Component
persistent identity: component1998445
display id: component1998445
version: 1
top level: BBa_K116669
definition: pRE
access: public

BBa_B0010
type: Component
persistent identity: component1998450
display id: component1998450
version: 1
top level: BBa_K116669
definition: BBa_B0010
access: public

BBa_K116601
type: Component
persistent identity: component1998449
display id: component1998449
version: 1
top level: BBa_K116669
definition: BBa_K116601
access: public

BBa_E0040
type: Component
persistent identity: component1998489
display id: component1998489
version: 1
top level: BBa_K116669
definition: GFP
access: public

BBa_B0032
type: Component
persistent identity: component1998486
display id: component1998486
version: 1
top level: BBa_K116669
definition: BBa_B0032
access: public

BBa_B0012
type: Component
persistent identity: component1998492
display id: component1998492
version: 1
top level: BBa_K116669
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1998490
display id: component1998490
version: 1
top level: BBa_K116669
definition: BBa_B0010
access: public

BBa_B0010
type: Component
persistent identity: component1998478
display id: component1998478
version: 1
top level: BBa_K116669
definition: BBa_B0010
access: public

BBa_K116609
type: Component
persistent identity: component1998477
display id: component1998477
version: 1
top level: BBa_K116669
definition: CIIICd
access: public

BBa_K116603
type: Component
persistent identity: component1998484
display id: component1998484
version: 1
top level: BBa_K116669
definition: pRE
access: public

BBa_B0012
type: Component
persistent identity: component1998480
display id: component1998480
version: 1
top level: BBa_K116669
definition: BBa_B0012
access: public

BBa_B0012
type: Component
persistent identity: component1998463
display id: component1998463
version: 1
top level: BBa_K116669
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1998461
display id: component1998461
version: 1
top level: BBa_K116669
definition: BBa_B0010
access: public

BBa_B0032
type: Component
persistent identity: component1998475
display id: component1998475
version: 1
top level: BBa_K116669
definition: BBa_B0032
access: public

BBa_K116603
type: SequenceAnnotation
persistent identity: annotation1998445
display id: annotation1998445
version: 1
top level: BBa_K116669
location: https://synbiohub.org/public/igem/BBa_K116669/annotation1998445/range1998445/1
component: BBa_K116603

https://synbiohub.org/public/igem/BBa_K116669/annotation1998445/range1998445/1
type: Range
persistent identity: range1998445
display id: range1998445
version: 1
top level: BBa_K116669
direction: direction/reverse
start: 1
end: 48
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1998447
display id: annotation1998447
version: 1
top level: BBa_K116669
location: https://synbiohub.org/public/igem/BBa_K116669/annotation1998447/range1998447/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K116669/annotation1998447/range1998447/1
type: Range
persistent identity: range1998447
display id: range1998447
version: 1
top level: BBa_K116669
direction: direction/forward
start: 57
end: 69
orientation: inline

BBa_K116601
type: SequenceAnnotation
persistent identity: annotation1998449
display id: annotation1998449
version: 1
top level: BBa_K116669
location: https://synbiohub.org/public/igem/BBa_K116669/annotation1998449/range1998449/1
component: BBa_K116601

https://synbiohub.org/public/igem/BBa_K116669/annotation1998449/range1998449/1
type: Range
persistent identity: range1998449
display id: range1998449
version: 1
top level: BBa_K116669
direction: direction/reverse
start: 76
end: 2010
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1998450
display id: annotation1998450
version: 1
top level: BBa_K116669
location: https://synbiohub.org/public/igem/BBa_K116669/annotation1998450/range1998450/1
component: BBa_B0010

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type: Range
persistent identity: range1998450
display id: range1998450
version: 1
top level: BBa_K116669
direction: direction/forward
start: 2019
end: 2098
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1998452
display id: annotation1998452
version: 1
top level: BBa_K116669
location: https://synbiohub.org/public/igem/BBa_K116669/annotation1998452/range1998452/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K116669/annotation1998452/range1998452/1
type: Range
persistent identity: range1998452
display id: range1998452
version: 1
top level: BBa_K116669
direction: direction/forward
start: 2107
end: 2147
orientation: inline

BBa_K116603
type: SequenceAnnotation
persistent identity: annotation1998456
display id: annotation1998456
version: 1
top level: BBa_K116669
location: https://synbiohub.org/public/igem/BBa_K116669/annotation1998456/range1998456/1
component: BBa_K116603

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type: Range
persistent identity: range1998456
display id: range1998456
version: 1
top level: BBa_K116669
direction: direction/reverse
start: 2156
end: 2203
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1998458
display id: annotation1998458
version: 1
top level: BBa_K116669
location: https://synbiohub.org/public/igem/BBa_K116669/annotation1998458/range1998458/1
component: BBa_B0032

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type: Range
persistent identity: range1998458
display id: range1998458
version: 1
top level: BBa_K116669
direction: direction/forward
start: 2212
end: 2224
orientation: inline

BBa_K116602
type: SequenceAnnotation
persistent identity: annotation1998460
display id: annotation1998460
version: 1
top level: BBa_K116669
location: https://synbiohub.org/public/igem/BBa_K116669/annotation1998460/range1998460/1
component: BBa_K116602

https://synbiohub.org/public/igem/BBa_K116669/annotation1998460/range1998460/1
type: Range
persistent identity: range1998460
display id: range1998460
version: 1
top level: BBa_K116669
direction: direction/reverse
start: 2231
end: 2524
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1998461
display id: annotation1998461
version: 1
top level: BBa_K116669
location: https://synbiohub.org/public/igem/BBa_K116669/annotation1998461/range1998461/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K116669/annotation1998461/range1998461/1
type: Range
persistent identity: range1998461
display id: range1998461
version: 1
top level: BBa_K116669
direction: direction/forward
start: 2533
end: 2612
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1998463
display id: annotation1998463
version: 1
top level: BBa_K116669
location: https://synbiohub.org/public/igem/BBa_K116669/annotation1998463/range1998463/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K116669/annotation1998463/range1998463/1
type: Range
persistent identity: range1998463
display id: range1998463
version: 1
top level: BBa_K116669
direction: direction/forward
start: 2621
end: 2661
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1998467
display id: annotation1998467
version: 1
top level: BBa_K116669
location: https://synbiohub.org/public/igem/BBa_K116669/annotation1998467/range1998467/1
component: BBa_R0010

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type: Range
persistent identity: range1998467
display id: range1998467
version: 1
top level: BBa_K116669
direction: direction/forward
start: 2670
end: 2869
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1998475
display id: annotation1998475
version: 1
top level: BBa_K116669
location: https://synbiohub.org/public/igem/BBa_K116669/annotation1998475/range1998475/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K116669/annotation1998475/range1998475/1
type: Range
persistent identity: range1998475
display id: range1998475
version: 1
top level: BBa_K116669
direction: direction/forward
start: 2878
end: 2890
orientation: inline

BBa_K116609
type: SequenceAnnotation
persistent identity: annotation1998477
display id: annotation1998477
version: 1
top level: BBa_K116669
location: https://synbiohub.org/public/igem/BBa_K116669/annotation1998477/range1998477/1
component: BBa_K116609

https://synbiohub.org/public/igem/BBa_K116669/annotation1998477/range1998477/1
type: Range
persistent identity: range1998477
display id: range1998477
version: 1
top level: BBa_K116669
direction: direction/reverse
start: 2897
end: 2986
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1998478
display id: annotation1998478
version: 1
top level: BBa_K116669
location: https://synbiohub.org/public/igem/BBa_K116669/annotation1998478/range1998478/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K116669/annotation1998478/range1998478/1
type: Range
persistent identity: range1998478
display id: range1998478
version: 1
top level: BBa_K116669
direction: direction/forward
start: 2995
end: 3074
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1998480
display id: annotation1998480
version: 1
top level: BBa_K116669
location: https://synbiohub.org/public/igem/BBa_K116669/annotation1998480/range1998480/1
component: BBa_B0012

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type: Range
persistent identity: range1998480
display id: range1998480
version: 1
top level: BBa_K116669
direction: direction/forward
start: 3083
end: 3123
orientation: inline

BBa_K116603
type: SequenceAnnotation
persistent identity: annotation1998484
display id: annotation1998484
version: 1
top level: BBa_K116669
location: https://synbiohub.org/public/igem/BBa_K116669/annotation1998484/range1998484/1
component: BBa_K116603

https://synbiohub.org/public/igem/BBa_K116669/annotation1998484/range1998484/1
type: Range
persistent identity: range1998484
display id: range1998484
version: 1
top level: BBa_K116669
direction: direction/reverse
start: 3132
end: 3179
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1998486
display id: annotation1998486
version: 1
top level: BBa_K116669
location: https://synbiohub.org/public/igem/BBa_K116669/annotation1998486/range1998486/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K116669/annotation1998486/range1998486/1
type: Range
persistent identity: range1998486
display id: range1998486
version: 1
top level: BBa_K116669
direction: direction/forward
start: 3188
end: 3200
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1998489
display id: annotation1998489
version: 1
top level: BBa_K116669
location: https://synbiohub.org/public/igem/BBa_K116669/annotation1998489/range1998489/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K116669/annotation1998489/range1998489/1
type: Range
persistent identity: range1998489
display id: range1998489
version: 1
top level: BBa_K116669
direction: direction/reverse
start: 3207
end: 3926
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1998490
display id: annotation1998490
version: 1
top level: BBa_K116669
location: https://synbiohub.org/public/igem/BBa_K116669/annotation1998490/range1998490/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K116669/annotation1998490/range1998490/1
type: Range
persistent identity: range1998490
display id: range1998490
version: 1
top level: BBa_K116669
direction: direction/forward
start: 3935
end: 4014
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1998492
display id: annotation1998492
version: 1
top level: BBa_K116669
location: https://synbiohub.org/public/igem/BBa_K116669/annotation1998492/range1998492/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K116669/annotation1998492/range1998492/1
type: Range
persistent identity: range1998492
display id: range1998492
version: 1
top level: BBa_K116669
direction: direction/forward
start: 4023
end: 4063
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_K116601
type: ComponentDefinition
persistent identity: BBa K116601
display id: BBa_K116601
version: 1
was generated by: iGEM to SBOL conversion
description: HtlB (ftsH) coding region from E. coli
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:33Z
mutable provenance: E. coli K. 12 MG1655
owned by: james
owned by: myers
mutable description: The HtlB (ftsH) coding region from E. coli. It can be used to degrade many different proteins.
discontinued: false
dominant: false
group u list: _210_
m user id: 0
owner id: 2749
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: PCR primer design
star: false
creator: Jesse Wu
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K116601_sequence/1

CII
type: ComponentDefinition
persistent identity: BBa K116602
display id: BBa_K116602
version: 1
was generated by: iGEM to SBOL conversion
description: CII coding region from λ phage
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:33Z
mutable provenance: λ phage
owned by: james
owned by: myers
mutable description: The CII coding region from λ phage.
discontinued: false
dominant: true
experience: experience/None
group u list: _210_
m user id: 0
owner id: 2749
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none.
star: false
creator: Jesse Wu
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K116602_sequence/1

pRE
type: ComponentDefinition
persistent identity: BBa K116603
display id: BBa_K116603
version: 1
was generated by: iGEM to SBOL conversion
description: pRE promoter from λ phage
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:33Z
mutable provenance: λ phage
owned by: james
owned by: myers
mutable description: The pRE coding region from λ phage. It is able to be induced by CII (BBa_K116602).
discontinued: false
dominant: false
experience: experience/None
group u list: _210_
m user id: 0
owner id: 2749
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none.
star: false
creator: Jesse Wu
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K116603_sequence/1

CIIICd
type: ComponentDefinition
persistent identity: BBa K116609
display id: BBa_K116609
version: 1
was generated by: iGEM to SBOL conversion
description: CIIICd: modified CIII coding region from λ phage
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:33Z
mutable provenance: λ phage
owned by: james
owned by: myers
mutable description: CIIICd: A modified version of the CIII coding region from ''λ phage''. '''Original CIII''': '''ATG'''CAATATGCCATTGCAGGGTGGCCTGTTGCTGGCTGC'''CCTTCCGAATCTTTACTTGAACGAATCACCC''' '''GTAAATTACGTGACGGATGGAAACGCCTTATCGACATACTTAATCAGCCAGGAG'''TCCCAAAGAATGGATC AAACACTTATGGCTATCCAGAC'''TAA''' The protein domains from the left-most and right-most side of CIII have been removed (the grayed out parts), i.e. amino acid residues #2-13 and #42-49 have been excluded, leaving residues #1 (start codon), #14-41 (the protein domain in the middle) and #55 (stop codon).
discontinued: false
dominant: false
experience: experience/None
group u list: _210_
m user id: 0
owner id: 2749
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: HtlB also degrades CIII, creating competitive degradation reactions. We removed the leftmost and right most protein domains since Hadler ''et al'' said that removing these two protein domains will stop HtlB from degrading CIII. Doing this makes it easier to predict and model.
star: false
creator: Jesse Wu
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K116609_sequence/1

https://synbiohub.org/public/igem/BBa_K116669_sequence/1
type: Sequence
persistent identity: BBa K116669 sequence
display id: BBa_K116669_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagcctcgttgcgtttgtttgcacgaaccatatgtaagtatttcctttactagagtcacacaggaaagtactagatggcgaaaaacctaatactctggctggtcattgccgttgtgctgatgtcagtattccagagctttgggcccagcgagtctaatggccgtaaggtggattactctaccttcctacaagaggtcaataacgaccaggttcgtgaagcgcgtatcaacggacgtgaaatcaacgttaccaagaaagatagtaaccgttataccacttacattccggttcaggatccgaaattactggataacctgttgaccaagaacgtcaaggttgtcggtgaaccgcctgaagaaccaagcctgctggcttctatcttcatctcctggttcccgatgctgttgctgattggtgtctggatcttcttcatgcgtcaaatgcagggcggcggtggcaaaggtgccatgtcgtttggtaagagcaaagcgcgcatgctgacggaagatcagatcaaaacgacctttgctgacgttgcgggctgcgacgaagcaaaagaagaagttgctgaactggttgagtatctgcgcgagccgagccgcttccagaaactcggcggtaagatcccgaaaggcgtcttgatggtcggtcctccgggtaccggtaaaacgctgctggcgaaagcgattgcaggcgaagcgaaagttccgttctttactatctccggttctgacttcgtagaaatgttcgtcggtgtgggtgcatcccgtgttcgtgacatgttcgaacaggcgaagaaagcggcaccgtgcatcatctttatcgatgaaatcgacgccgtaggccgccagcgtggcgctggtctgggcggtggtcacgatgaacgtgaacagactctgaaccagatgctggttgagatggatggcttcgaaggtaacgaaggtatcatcgttatcgccgcgactaaccgtccggacgttctcgacccggccctgctgcgtcctggccgtttcgaccgtcaggttgtggtcggcttgccagatgttcgcggtcgtgagcagatcctgaaagttcacatgcgtcgcgtaccattggcacccgatatcgacgcggcaatcattgcccgtggtactcctggtttctccggtgctgacctggcgaacctggtgaacgaagcggcactgttcgctgctcgtggcaacaaacgcgttgtgtcgatggttgagttcgagaaagcgaaagacaaaatcatgatgggtgcggaacgtcgctccatggtgatgacggaagcgcagaaagaatcgacggcttaccacgaagcgggtcatgcgattatcggtcgcctggtgccggaacacgatccggtgcacaaagtgacgattatcccacgcggtcgtgcgctgggtgtgactttcttcttgcctgagggcgacgcaatcagcgccagccgtcagaaactggaaagccagatttctacgctgtacggtggtcgtctggcagaagagatcatctacgggccggaacatgtatctaccggtgcgtccaacgatattaaagttgcgaccaacctggcacgtaacatggtgactcagtggggcttctctgagaaattgggtccactgctgtacgcggaagaagaaggtgaagtgttcctcggccgtagcgtagcgaaagcgaaacatatgtccgatgaaactgcacgtatcatcgaccaggaagtgaaagcactgattgagcgtaactataatcgtgcgcgtcagcttctgaccgacaatatggatattctgcatgcgatgaaagatgctctcatgaaatatgagactatcgacgcaccgcagattgatgacctgatggcacgtcgcgatgtacgtccgccagcgggctgggaagaaccaggcgcttctaacaattctggcgacaatggtagtccaaaggctcctcgtccggttgatgaaccgcgtacgccgaacccgggtaacaccatgtcagagcagttagg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K116601_sequence/1
type: Sequence
persistent identity: BBa K116601 sequence
display id: BBa_K116601_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcgaaaaacctaatactctggctggtcattgccgttgtgctgatgtcagtattccagagctttgggcccagcgagtctaatggccgtaaggtggattactctaccttcctacaagaggtcaataacgaccaggttcgtgaagcgcgtatcaacggacgtgaaatcaacgttaccaagaaagatagtaaccgttataccacttacattccggttcaggatccgaaattactggataacctgttgaccaagaacgtcaaggttgtcggtgaaccgcctgaagaaccaagcctgctggcttctatcttcatctcctggttcccgatgctgttgctgattggtgtctggatcttcttcatgcgtcaaatgcagggcggcggtggcaaaggtgccatgtcgtttggtaagagcaaagcgcgcatgctgacggaagatcagatcaaaacgacctttgctgacgttgcgggctgcgacgaagcaaaagaagaagttgctgaactggttgagtatctgcgcgagccgagccgcttccagaaactcggcggtaagatcccgaaaggcgtcttgatggtcggtcctccgggtaccggtaaaacgctgctggcgaaagcgattgcaggcgaagcgaaagttccgttctttactatctccggttctgacttcgtagaaatgttcgtcggtgtgggtgcatcccgtgttcgtgacatgttcgaacaggcgaagaaagcggcaccgtgcatcatctttatcgatgaaatcgacgccgtaggccgccagcgtggcgctggtctgggcggtggtcacgatgaacgtgaacagactctgaaccagatgctggttgagatggatggcttcgaaggtaacgaaggtatcatcgttatcgccgcgactaaccgtccggacgttctcgacccggccctgctgcgtcctggccgtttcgaccgtcaggttgtggtcggcttgccagatgttcgcggtcgtgagcagatcctgaaagttcacatgcgtcgcgtaccattggcacccgatatcgacgcggcaatcattgcccgtggtactcctggtttctccggtgctgacctggcgaacctggtgaacgaagcggcactgttcgctgctcgtggcaacaaacgcgttgtgtcgatggttgagttcgagaaagcgaaagacaaaatcatgatgggtgcggaacgtcgctccatggtgatgacggaagcgcagaaagaatcgacggcttaccacgaagcgggtcatgcgattatcggtcgcctggtgccggaacacgatccggtgcacaaagtgacgattatcccacgcggtcgtgcgctgggtgtgactttcttcttgcctgagggcgacgcaatcagcgccagccgtcagaaactggaaagccagatttctacgctgtacggtggtcgtctggcagaagagatcatctacgggccggaacatgtatctaccggtgcgtccaacgatattaaagttgcgaccaacctggcacgtaacatggtgactcagtggggcttctctgagaaattgggtccactgctgtacgcggaagaagaaggtgaagtgttcctcggccgtagcgtagcgaaagcgaaacatatgtccgatgaaactgcacgtatcatcgaccaggaagtgaaagcactgattgagcgtaactataatcgtgcgcgtcagcttctgaccgacaatatggatattctgcatgcgatgaaagatgctctcatgaaatatgagactatcgacgcaccgcagattgatgacctgatggcacgtcgcgatgtacgtccgccagcgggctgggaagaaccaggcgcttctaacaattctggcgacaatggtagtccaaaggctcctcgtccggttgatgaaccgcgtacgccgaacccgggtaacaccatgtcagagcagttaggcgacaagtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K116602_sequence/1
type: Sequence
persistent identity: BBa K116602 sequence
display id: BBa_K116602_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggttcgtgcaaacaaacgcaacgaggctctacgaatcgagagtgcgttgcttaacaaaatcgcaatgcttggaactgagaagacagcggaagctgtgggcgttgataagtcgcagatcagcaggtggaagagggactggattccaaagttctcaatgctgcttgctgttcttgaatggggggtcgttgacgacgacatggctcgattggcgcgacaagttgctgcgattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K116603_sequence/1
type: Sequence
persistent identity: BBa K116603 sequence
display id: BBa_K116603_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagcctcgttgcgtttgtttgcacgaaccatatgtaagtatttcctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K116609_sequence/1
type: Sequence
persistent identity: BBa K116609 sequence
display id: BBa_K116609_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgccttccgaatctttacttgaacgaatcacccgtaaattacgtgacggatggaaacgccttatcgacatacttaatcagccaggataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z