LOCUS BBa_K116675 6255 bp DNA linear 05-Nov-2024 DEFINITION Short Synchronizer + Oscillator + Tuner + Reporter ACCESSION BBa_K116675 VERSION BBa_K116675.1 FEATURES Location/Qualifiers promoter 1..48 /label=BBa_K116603 RBS 57..69 /label=RBS-3\Weak RBS 63..66 /label=RBS misc_feature 57..69 /label=BBa_B0032 RBS 57..69 /label=BBa_B0032 misc_feature 76..77 /label=prefix misc_feature 249..249 /label=T misc_feature 567..567 /label=A CDS 76..825 /label=luxR misc_feature 76..831 /label=BBa_C0062 misc_feature 832..856 /label=Help:Barcodes CDS 76..831 /label=BBa_C0062 misc_feature 57..856 /label=BBa_K116616 RBS 865..877 /label=RBS-3\Weak RBS 871..874 /label=RBS misc_feature 865..877 /label=BBa_B0032 RBS 865..877 /label=BBa_B0032 misc_feature 1463..1494 /label=LVA CDS 884..1462 /label=luxI misc_feature 884..1501 /label=BBa_C0061 misc_feature 1502..1526 /label=Help:Barcodes CDS 884..1501 /label=BBa_C0061 stem_loop 1546..1589 /label=stem_loop misc_feature 1535..1614 /label=BBa_B0010 terminator 1535..1614 /label=BBa_B0010 stem_loop 1630..1649 /label=T7 TE misc_feature 1656..1656 /label=stop polyA_site 1650..1663 /label=polya misc_feature 1623..1663 /label=BBa_B0012 terminator 1623..1663 /label=BBa_B0012 terminator 1535..1663 /label=BBa_B0015 misc_feature 884..1663 /label=BBa_K116615 misc_feature 865..1663 /label=BBa_K116625 misc_feature 57..1663 /label=BBa_K116634 misc_feature 1672..1691 /label=LuxR/HSL promoter 1691..1696 /label=-35 promoter 1713..1718 /label=-10 misc_feature 1724..1724 /label=start misc_feature 1672..1726 /label=BBa_R0062 promoter 1672..1726 /label=BBa_R0062 misc_feature 57..1726 /label=BBa_K116645 misc_feature 1..1726 /label=BBa_K116655 RBS 1735..1747 /label=RBS-3\Weak RBS 1741..1744 /label=RBS misc_feature 1735..1747 /label=BBa_B0032 RBS 1735..1747 /label=BBa_B0032 CDS 1754..2047 /label=BBa_K116602 stem_loop 2067..2110 /label=stem_loop misc_feature 2056..2135 /label=BBa_B0010 terminator 2056..2135 /label=BBa_B0010 stem_loop 2151..2170 /label=T7 TE misc_feature 2177..2177 /label=stop polyA_site 2171..2184 /label=polya misc_feature 2144..2184 /label=BBa_B0012 terminator 2144..2184 /label=BBa_B0012 misc_feature 1735..2184 /label=BBa_K116622 misc_feature 1..2184 /label=BBa_K116665 promoter 2193..2240 /label=BBa_K116603 RBS 2249..2261 /label=RBS-3\Weak RBS 2255..2258 /label=RBS misc_feature 2249..2261 /label=BBa_B0032 RBS 2249..2261 /label=BBa_B0032 CDS 2268..4202 /label=BBa_K116601 stem_loop 4222..4265 /label=stem_loop misc_feature 4211..4290 /label=BBa_B0010 terminator 4211..4290 /label=BBa_B0010 stem_loop 4306..4325 /label=T7 TE misc_feature 4332..4332 /label=stop polyA_site 4326..4339 /label=polya misc_feature 4299..4339 /label=BBa_B0012 terminator 4299..4339 /label=BBa_B0012 promoter 4348..4395 /label=BBa_K116603 RBS 4404..4416 /label=RBS-3\Weak RBS 4410..4413 /label=RBS misc_feature 4404..4416 /label=BBa_B0032 RBS 4404..4416 /label=BBa_B0032 CDS 4423..4716 /label=BBa_K116602 stem_loop 4736..4779 /label=stem_loop misc_feature 4725..4804 /label=BBa_B0010 terminator 4725..4804 /label=BBa_B0010 stem_loop 4820..4839 /label=T7 TE misc_feature 4846..4846 /label=stop polyA_site 4840..4853 /label=polya misc_feature 4813..4853 /label=BBa_B0012 terminator 4813..4853 /label=BBa_B0012 CDS 4862..4949 /label=end of LacI coding region (inactive) misc_feature 4862..5061 /label=BBa_R0010 misc_feature 4950..4987 /label=CAP binding site promoter 4998..5003 /label=-35 promoter 5022..5027 /label=-10 misc_feature 5027..5061 /label=LacI binding site misc_feature 5034..5034 /label=start promoter 4862..5061 /label=BBa_R0010 RBS 5070..5082 /label=RBS-3\Weak RBS 5076..5079 /label=RBS misc_feature 5070..5082 /label=BBa_B0032 RBS 5070..5082 /label=BBa_B0032 CDS 5089..5178 /label=BBa_K116609 stem_loop 5198..5241 /label=stem_loop misc_feature 5187..5266 /label=BBa_B0010 terminator 5187..5266 /label=BBa_B0010 stem_loop 5282..5301 /label=T7 TE misc_feature 5308..5308 /label=stop polyA_site 5302..5315 /label=polya misc_feature 5275..5315 /label=BBa_B0012 terminator 5275..5315 /label=BBa_B0012 promoter 5324..5371 /label=BBa_K116603 RBS 5380..5392 /label=RBS-3\Weak RBS 5386..5389 /label=RBS misc_feature 5380..5392 /label=BBa_B0032 RBS 5380..5392 /label=BBa_B0032 CDS 5399..6118 /label=GFP protein CDS 5399..6118 /label=BBa_E0040 stem_loop 6138..6181 /label=stem_loop misc_feature 6127..6206 /label=BBa_B0010 terminator 6127..6206 /label=BBa_B0010 stem_loop 6222..6241 /label=T7 TE misc_feature 6248..6248 /label=stop polyA_site 6242..6255 /label=polya misc_feature 6215..6255 /label=BBa_B0012 terminator 6215..6255 /label=BBa_B0012 misc_feature 2193..6255 /label=BBa_K116669 ORIGIN 1 agagcctcgt tgcgtttgtt tgcacgaacc atatgtaagt atttccttta ctagagtcac 61 acaggaaagt actagatgaa aaacataaat gccgacgaca catacagaat aattaataaa 121 attaaagctt gtagaagcaa taatgatatt aatcaatgct tatctgatat gactaaaatg 181 gtacattgtg aatattattt actcgcgatc atttatcctc attctatggt taaatctgat 241 atttcaatcc tagataatta ccctaaaaaa tggaggcaat attatgatga cgctaattta 301 ataaaatatg atcctatagt agattattct aactccaatc attcaccaat taattggaat 361 atatttgaaa acaatgctgt aaataaaaaa tctccaaatg taattaaaga agcgaaaaca 421 tcaggtctta tcactgggtt tagtttccct attcatacgg ctaacaatgg cttcggaatg 481 cttagttttg cacattcaga aaaagacaac tatatagata gtttattttt acatgcgtgt 541 atgaacatac cattaattgt tccttctcta gttgataatt atcgaaaaat aaatatagca 601 aataataaat caaacaacga tttaaccaaa agagaaaaag aatgtttagc gtgggcatgc 661 gaaggaaaaa gctcttggga tatttcaaaa atattaggtt gcagtgagcg tactgtcact 721 ttccatttaa ccaatgcgca aatgaaactc aatacaacaa accgctgcca aagtatttct 781 aaagcaattt taacaggagc aattgattgc ccatacttta aaaattaata acactgatag 841 tgctagtgta gatcactact agagtcacac aggaaagtac tagatgacta taatgataaa 901 aaaatcggat tttttggcaa ttccatcgga ggagtataaa ggtattctaa gtcttcgtta 961 tcaagtgttt aagcaaagac ttgagtggga cttagttgta gaaaataacc ttgaatcaga 1021 tgagtatgat aactcaaatg cagaatatat ttatgcttgt gatgatactg aaaatgtaag 1081 tggatgctgg cgtttattac ctacaacagg tgattatatg ctgaaaagtg tttttcctga 1141 attgcttggt caacagagtg ctcccaaaga tcctaatata gtcgaattaa gtcgttttgc 1201 tgtaggtaaa aatagctcaa agataaataa ctctgctagt gaaattacaa tgaaactatt 1261 tgaagctata tataaacacg ctgttagtca aggtattaca gaatatgtaa cagtaacatc 1321 aacagcaata gagcgatttt taaagcgtat taaagttcct tgtcatcgta ttggagacaa 1381 agaaattcat gtattaggtg atactaaatc ggttgtattg tctatgccta ttaatgaaca 1441 gtttaaaaaa gcagtcttaa atgctgcaaa cgacgaaaac tacgctttag tagcttaata 1501 actctgatag tgctagtgta gatctctact agagccaggc atcaaataaa acgaaaggct 1561 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 1621 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gacctgtagg 1681 atcgtacagg tttacgcaag aaaatggttt gttatagtcg aataaatact agagtcacac 1741 aggaaagtac tagatggttc gtgcaaacaa acgcaacgag gctctacgaa tcgagagtgc 1801 gttgcttaac aaaatcgcaa tgcttggaac tgagaagaca gcggaagctg tgggcgttga 1861 taagtcgcag atcagcaggt ggaagaggga ctggattcca aagttctcaa tgctgcttgc 1921 tgttcttgaa tggggggtcg ttgacgacga catggctcga ttggcgcgac aagttgctgc 1981 gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga 2041 gttctgatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 2101 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 2161 cgggtgggcc tttctgcgtt tatatactag agagagcctc gttgcgtttg tttgcacgaa 2221 ccatatgtaa gtatttcctt tactagagtc acacaggaaa gtactagatg gcgaaaaacc 2281 taatactctg gctggtcatt gccgttgtgc tgatgtcagt attccagagc tttgggccca 2341 gcgagtctaa tggccgtaag gtggattact ctaccttcct acaagaggtc aataacgacc 2401 aggttcgtga agcgcgtatc aacggacgtg aaatcaacgt taccaagaaa gatagtaacc 2461 gttataccac ttacattccg gttcaggatc cgaaattact ggataacctg ttgaccaaga 2521 acgtcaaggt tgtcggtgaa ccgcctgaag aaccaagcct gctggcttct atcttcatct 2581 cctggttccc gatgctgttg ctgattggtg tctggatctt cttcatgcgt caaatgcagg 2641 gcggcggtgg caaaggtgcc atgtcgtttg gtaagagcaa agcgcgcatg ctgacggaag 2701 atcagatcaa aacgaccttt gctgacgttg cgggctgcga cgaagcaaaa gaagaagttg 2761 ctgaactggt tgagtatctg cgcgagccga gccgcttcca gaaactcggc ggtaagatcc 2821 cgaaaggcgt cttgatggtc ggtcctccgg gtaccggtaa aacgctgctg gcgaaagcga 2881 ttgcaggcga agcgaaagtt ccgttcttta ctatctccgg ttctgacttc gtagaaatgt 2941 tcgtcggtgt gggtgcatcc cgtgttcgtg acatgttcga acaggcgaag aaagcggcac 3001 cgtgcatcat ctttatcgat gaaatcgacg ccgtaggccg ccagcgtggc gctggtctgg 3061 gcggtggtca cgatgaacgt gaacagactc tgaaccagat gctggttgag atggatggct 3121 tcgaaggtaa cgaaggtatc atcgttatcg ccgcgactaa ccgtccggac gttctcgacc 3181 cggccctgct gcgtcctggc cgtttcgacc gtcaggttgt ggtcggcttg ccagatgttc 3241 gcggtcgtga gcagatcctg aaagttcaca tgcgtcgcgt accattggca cccgatatcg 3301 acgcggcaat cattgcccgt ggtactcctg gtttctccgg tgctgacctg gcgaacctgg 3361 tgaacgaagc ggcactgttc gctgctcgtg gcaacaaacg cgttgtgtcg atggttgagt 3421 tcgagaaagc gaaagacaaa atcatgatgg gtgcggaacg tcgctccatg gtgatgacgg 3481 aagcgcagaa agaatcgacg gcttaccacg aagcgggtca tgcgattatc ggtcgcctgg 3541 tgccggaaca cgatccggtg cacaaagtga cgattatccc acgcggtcgt gcgctgggtg 3601 tgactttctt cttgcctgag ggcgacgcaa tcagcgccag ccgtcagaaa ctggaaagcc 3661 agatttctac gctgtacggt ggtcgtctgg cagaagagat catctacggg ccggaacatg 3721 tatctaccgg tgcgtccaac gatattaaag ttgcgaccaa cctggcacgt aacatggtga 3781 ctcagtgggg cttctctgag aaattgggtc cactgctgta cgcggaagaa gaaggtgaag 3841 tgttcctcgg ccgtagcgta gcgaaagcga aacatatgtc cgatgaaact gcacgtatca 3901 tcgaccagga agtgaaagca ctgattgagc gtaactataa tcgtgcgcgt cagcttctga 3961 ccgacaatat ggatattctg catgcgatga aagatgctct catgaaatat gagactatcg 4021 acgcaccgca gattgatgac ctgatggcac gtcgcgatgt acgtccgcca gcgggctggg 4081 aagaaccagg cgcttctaac aattctggcg acaatggtag tccaaaggct cctcgtccgg 4141 ttgatgaacc gcgtacgccg aacccgggta acaccatgtc agagcagtta ggcgacaagt 4201 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 4261 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 4321 gggcctttct gcgtttatat actagagaga gcctcgttgc gtttgtttgc acgaaccata 4381 tgtaagtatt tcctttacta gagtcacaca ggaaagtact agatggttcg tgcaaacaaa 4441 cgcaacgagg ctctacgaat cgagagtgcg ttgcttaaca aaatcgcaat gcttggaact 4501 gagaagacag cggaagctgt gggcgttgat aagtcgcaga tcagcaggtg gaagagggac 4561 tggattccaa agttctcaat gctgcttgct gttcttgaat ggggggtcgt tgacgacgac 4621 atggctcgat tggcgcgaca agttgctgcg attctcacca ataaaaaacg cccggcggca 4681 accgagcgtt ctgaacaaat ccagatggag ttctgatact agagccaggc atcaaataaa 4741 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 4801 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga 4861 gcaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 4921 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 4981 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg 5041 agcggataac aatttcacac atactagagt cacacaggaa agtactagat gccttccgaa 5101 tctttacttg aacgaatcac ccgtaaatta cgtgacggat ggaaacgcct tatcgacata 5161 cttaatcagc caggataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 5221 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 5281 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagagagcct cgttgcgttt 5341 gtttgcacga accatatgta agtatttcct ttactagagt cacacaggaa agtactagat 5401 gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga 5461 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa 5521 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt 5581 cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca 5641 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa 5701 agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa 5761 tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt 5821 ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat 5881 caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca 5941 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct 6001 gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct 6061 tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca aataataata 6121 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 6181 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 6241 ctttctgcgt ttata //