LOCUS BBa_K116676 6454 bp DNA linear 05-Nov-2024 DEFINITION Synchronizer + Oscillator + Tuner + Reporter ACCESSION BBa_K116676 VERSION BBa_K116676.1 FEATURES Location/Qualifiers promoter 1..48 /label=BBa_K116603 RBS 57..69 /label=RBS-3\Weak RBS 63..66 /label=RBS misc_feature 57..69 /label=BBa_B0032 RBS 57..69 /label=BBa_B0032 misc_feature 655..686 /label=LVA CDS 76..654 /label=luxI misc_feature 76..693 /label=BBa_C0061 misc_feature 694..718 /label=Help:Barcodes CDS 76..693 /label=BBa_C0061 stem_loop 738..781 /label=stem_loop misc_feature 727..806 /label=BBa_B0010 terminator 727..806 /label=BBa_B0010 stem_loop 822..841 /label=T7 TE misc_feature 848..848 /label=stop polyA_site 842..855 /label=polya misc_feature 815..855 /label=BBa_B0012 terminator 815..855 /label=BBa_B0012 terminator 727..855 /label=BBa_B0015 misc_feature 76..855 /label=BBa_K116615 misc_feature 57..855 /label=BBa_K116625 misc_feature 1..855 /label=BBa_K116635 misc_feature 864..882 /label=TetR 1 misc_feature 864..917 /label=BBa_R0040 promoter 883..888 /label=-35 misc_feature 889..907 /label=TetR 2 promoter 906..911 /label=-10 promoter 864..917 /label=BBa_R0040 RBS 926..938 /label=RBS-3\Weak RBS 932..935 /label=RBS misc_feature 926..938 /label=BBa_B0032 RBS 926..938 /label=BBa_B0032 misc_feature 945..946 /label=prefix misc_feature 1118..1118 /label=T misc_feature 1436..1436 /label=A CDS 945..1694 /label=luxR misc_feature 945..1700 /label=BBa_C0062 misc_feature 1701..1725 /label=Help:Barcodes CDS 945..1700 /label=BBa_C0062 misc_feature 926..1725 /label=BBa_K116616 stem_loop 1745..1788 /label=stem_loop misc_feature 1734..1813 /label=BBa_B0010 terminator 1734..1813 /label=BBa_B0010 stem_loop 1829..1848 /label=T7 TE misc_feature 1855..1855 /label=stop polyA_site 1849..1862 /label=polya misc_feature 1822..1862 /label=BBa_B0012 terminator 1822..1862 /label=BBa_B0012 terminator 1734..1862 /label=BBa_B0015 misc_feature 926..1862 /label=BBa_K116626 misc_feature 864..1862 /label=BBa_K116640 misc_feature 1871..1890 /label=LuxR/HSL promoter 1890..1895 /label=-35 promoter 1912..1917 /label=-10 misc_feature 1923..1923 /label=start misc_feature 1871..1925 /label=BBa_R0062 promoter 1871..1925 /label=BBa_R0062 RBS 1934..1946 /label=RBS-3\Weak RBS 1940..1943 /label=RBS misc_feature 1934..1946 /label=BBa_B0032 RBS 1934..1946 /label=BBa_B0032 CDS 1953..2246 /label=BBa_K116602 stem_loop 2266..2309 /label=stem_loop misc_feature 2255..2334 /label=BBa_B0010 terminator 2255..2334 /label=BBa_B0010 stem_loop 2350..2369 /label=T7 TE misc_feature 2376..2376 /label=stop polyA_site 2370..2383 /label=polya misc_feature 2343..2383 /label=BBa_B0012 terminator 2343..2383 /label=BBa_B0012 misc_feature 1871..2383 /label=BBa_K116637 misc_feature 864..2383 /label=BBa_K116646 misc_feature 1..2383 /label=BBa_K116656 promoter 2392..2439 /label=BBa_K116603 RBS 2448..2460 /label=RBS-3\Weak RBS 2454..2457 /label=RBS misc_feature 2448..2460 /label=BBa_B0032 RBS 2448..2460 /label=BBa_B0032 CDS 2467..4401 /label=BBa_K116601 stem_loop 4421..4464 /label=stem_loop misc_feature 4410..4489 /label=BBa_B0010 terminator 4410..4489 /label=BBa_B0010 stem_loop 4505..4524 /label=T7 TE misc_feature 4531..4531 /label=stop polyA_site 4525..4538 /label=polya misc_feature 4498..4538 /label=BBa_B0012 terminator 4498..4538 /label=BBa_B0012 promoter 4547..4594 /label=BBa_K116603 RBS 4603..4615 /label=RBS-3\Weak RBS 4609..4612 /label=RBS misc_feature 4603..4615 /label=BBa_B0032 RBS 4603..4615 /label=BBa_B0032 CDS 4622..4915 /label=BBa_K116602 stem_loop 4935..4978 /label=stem_loop misc_feature 4924..5003 /label=BBa_B0010 terminator 4924..5003 /label=BBa_B0010 stem_loop 5019..5038 /label=T7 TE misc_feature 5045..5045 /label=stop polyA_site 5039..5052 /label=polya misc_feature 5012..5052 /label=BBa_B0012 terminator 5012..5052 /label=BBa_B0012 CDS 5061..5148 /label=end of LacI coding region (inactive) misc_feature 5061..5260 /label=BBa_R0010 misc_feature 5149..5186 /label=CAP binding site promoter 5197..5202 /label=-35 promoter 5221..5226 /label=-10 misc_feature 5226..5260 /label=LacI binding site misc_feature 5233..5233 /label=start promoter 5061..5260 /label=BBa_R0010 RBS 5269..5281 /label=RBS-3\Weak RBS 5275..5278 /label=RBS misc_feature 5269..5281 /label=BBa_B0032 RBS 5269..5281 /label=BBa_B0032 CDS 5288..5377 /label=BBa_K116609 stem_loop 5397..5440 /label=stem_loop misc_feature 5386..5465 /label=BBa_B0010 terminator 5386..5465 /label=BBa_B0010 stem_loop 5481..5500 /label=T7 TE misc_feature 5507..5507 /label=stop polyA_site 5501..5514 /label=polya misc_feature 5474..5514 /label=BBa_B0012 terminator 5474..5514 /label=BBa_B0012 promoter 5523..5570 /label=BBa_K116603 RBS 5579..5591 /label=RBS-3\Weak RBS 5585..5588 /label=RBS misc_feature 5579..5591 /label=BBa_B0032 RBS 5579..5591 /label=BBa_B0032 CDS 5598..6317 /label=GFP protein CDS 5598..6317 /label=BBa_E0040 stem_loop 6337..6380 /label=stem_loop misc_feature 6326..6405 /label=BBa_B0010 terminator 6326..6405 /label=BBa_B0010 stem_loop 6421..6440 /label=T7 TE misc_feature 6447..6447 /label=stop polyA_site 6441..6454 /label=polya misc_feature 6414..6454 /label=BBa_B0012 terminator 6414..6454 /label=BBa_B0012 misc_feature 2392..6454 /label=BBa_K116669 ORIGIN 1 agagcctcgt tgcgtttgtt tgcacgaacc atatgtaagt atttccttta ctagagtcac 61 acaggaaagt actagatgac tataatgata aaaaaatcgg attttttggc aattccatcg 121 gaggagtata aaggtattct aagtcttcgt tatcaagtgt ttaagcaaag acttgagtgg 181 gacttagttg tagaaaataa ccttgaatca gatgagtatg ataactcaaa tgcagaatat 241 atttatgctt gtgatgatac tgaaaatgta agtggatgct ggcgtttatt acctacaaca 301 ggtgattata tgctgaaaag tgtttttcct gaattgcttg gtcaacagag tgctcccaaa 361 gatcctaata tagtcgaatt aagtcgtttt gctgtaggta aaaatagctc aaagataaat 421 aactctgcta gtgaaattac aatgaaacta tttgaagcta tatataaaca cgctgttagt 481 caaggtatta cagaatatgt aacagtaaca tcaacagcaa tagagcgatt tttaaagcgt 541 attaaagttc cttgtcatcg tattggagac aaagaaattc atgtattagg tgatactaaa 601 tcggttgtat tgtctatgcc tattaatgaa cagtttaaaa aagcagtctt aaatgctgca 661 aacgacgaaa actacgcttt agtagcttaa taactctgat agtgctagtg tagatctcta 721 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 781 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 841 ctttctgcgt ttatatacta gagtccctat cagtgataga gattgacatc cctatcagtg 901 atagagatac tgagcactac tagagtcaca caggaaagta ctagatgaaa aacataaatg 961 ccgacgacac atacagaata attaataaaa ttaaagcttg tagaagcaat aatgatatta 1021 atcaatgctt atctgatatg actaaaatgg tacattgtga atattattta ctcgcgatca 1081 tttatcctca ttctatggtt aaatctgata tttcaatcct agataattac cctaaaaaat 1141 ggaggcaata ttatgatgac gctaatttaa taaaatatga tcctatagta gattattcta 1201 actccaatca ttcaccaatt aattggaata tatttgaaaa caatgctgta aataaaaaat 1261 ctccaaatgt aattaaagaa gcgaaaacat caggtcttat cactgggttt agtttcccta 1321 ttcatacggc taacaatggc ttcggaatgc ttagttttgc acattcagaa aaagacaact 1381 atatagatag tttattttta catgcgtgta tgaacatacc attaattgtt ccttctctag 1441 ttgataatta tcgaaaaata aatatagcaa ataataaatc aaacaacgat ttaaccaaaa 1501 gagaaaaaga atgtttagcg tgggcatgcg aaggaaaaag ctcttgggat atttcaaaaa 1561 tattaggttg cagtgagcgt actgtcactt tccatttaac caatgcgcaa atgaaactca 1621 atacaacaaa ccgctgccaa agtatttcta aagcaatttt aacaggagca attgattgcc 1681 catactttaa aaattaataa cactgatagt gctagtgtag atcactacta gagccaggca 1741 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 1801 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 1861 tatactagag acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga 1921 ataaatacta gagtcacaca ggaaagtact agatggttcg tgcaaacaaa cgcaacgagg 1981 ctctacgaat cgagagtgcg ttgcttaaca aaatcgcaat gcttggaact gagaagacag 2041 cggaagctgt gggcgttgat aagtcgcaga tcagcaggtg gaagagggac tggattccaa 2101 agttctcaat gctgcttgct gttcttgaat ggggggtcgt tgacgacgac atggctcgat 2161 tggcgcgaca agttgctgcg attctcacca ataaaaaacg cccggcggca accgagcgtt 2221 ctgaacaaat ccagatggag ttctgatact agagccaggc atcaaataaa acgaaaggct 2281 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 2341 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gagagcctcg 2401 ttgcgtttgt ttgcacgaac catatgtaag tatttccttt actagagtca cacaggaaag 2461 tactagatgg cgaaaaacct aatactctgg ctggtcattg ccgttgtgct gatgtcagta 2521 ttccagagct ttgggcccag cgagtctaat ggccgtaagg tggattactc taccttccta 2581 caagaggtca ataacgacca ggttcgtgaa gcgcgtatca acggacgtga aatcaacgtt 2641 accaagaaag atagtaaccg ttataccact tacattccgg ttcaggatcc gaaattactg 2701 gataacctgt tgaccaagaa cgtcaaggtt gtcggtgaac cgcctgaaga accaagcctg 2761 ctggcttcta tcttcatctc ctggttcccg atgctgttgc tgattggtgt ctggatcttc 2821 ttcatgcgtc aaatgcaggg cggcggtggc aaaggtgcca tgtcgtttgg taagagcaaa 2881 gcgcgcatgc tgacggaaga tcagatcaaa acgacctttg ctgacgttgc gggctgcgac 2941 gaagcaaaag aagaagttgc tgaactggtt gagtatctgc gcgagccgag ccgcttccag 3001 aaactcggcg gtaagatccc gaaaggcgtc ttgatggtcg gtcctccggg taccggtaaa 3061 acgctgctgg cgaaagcgat tgcaggcgaa gcgaaagttc cgttctttac tatctccggt 3121 tctgacttcg tagaaatgtt cgtcggtgtg ggtgcatccc gtgttcgtga catgttcgaa 3181 caggcgaaga aagcggcacc gtgcatcatc tttatcgatg aaatcgacgc cgtaggccgc 3241 cagcgtggcg ctggtctggg cggtggtcac gatgaacgtg aacagactct gaaccagatg 3301 ctggttgaga tggatggctt cgaaggtaac gaaggtatca tcgttatcgc cgcgactaac 3361 cgtccggacg ttctcgaccc ggccctgctg cgtcctggcc gtttcgaccg tcaggttgtg 3421 gtcggcttgc cagatgttcg cggtcgtgag cagatcctga aagttcacat gcgtcgcgta 3481 ccattggcac ccgatatcga cgcggcaatc attgcccgtg gtactcctgg tttctccggt 3541 gctgacctgg cgaacctggt gaacgaagcg gcactgttcg ctgctcgtgg caacaaacgc 3601 gttgtgtcga tggttgagtt cgagaaagcg aaagacaaaa tcatgatggg tgcggaacgt 3661 cgctccatgg tgatgacgga agcgcagaaa gaatcgacgg cttaccacga agcgggtcat 3721 gcgattatcg gtcgcctggt gccggaacac gatccggtgc acaaagtgac gattatccca 3781 cgcggtcgtg cgctgggtgt gactttcttc ttgcctgagg gcgacgcaat cagcgccagc 3841 cgtcagaaac tggaaagcca gatttctacg ctgtacggtg gtcgtctggc agaagagatc 3901 atctacgggc cggaacatgt atctaccggt gcgtccaacg atattaaagt tgcgaccaac 3961 ctggcacgta acatggtgac tcagtggggc ttctctgaga aattgggtcc actgctgtac 4021 gcggaagaag aaggtgaagt gttcctcggc cgtagcgtag cgaaagcgaa acatatgtcc 4081 gatgaaactg cacgtatcat cgaccaggaa gtgaaagcac tgattgagcg taactataat 4141 cgtgcgcgtc agcttctgac cgacaatatg gatattctgc atgcgatgaa agatgctctc 4201 atgaaatatg agactatcga cgcaccgcag attgatgacc tgatggcacg tcgcgatgta 4261 cgtccgccag cgggctggga agaaccaggc gcttctaaca attctggcga caatggtagt 4321 ccaaaggctc ctcgtccggt tgatgaaccg cgtacgccga acccgggtaa caccatgtca 4381 gagcagttag gcgacaagta atactagagc caggcatcaa ataaaacgaa aggctcagtc 4441 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 4501 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagagag cctcgttgcg 4561 tttgtttgca cgaaccatat gtaagtattt cctttactag agtcacacag gaaagtacta 4621 gatggttcgt gcaaacaaac gcaacgaggc tctacgaatc gagagtgcgt tgcttaacaa 4681 aatcgcaatg cttggaactg agaagacagc ggaagctgtg ggcgttgata agtcgcagat 4741 cagcaggtgg aagagggact ggattccaaa gttctcaatg ctgcttgctg ttcttgaatg 4801 gggggtcgtt gacgacgaca tggctcgatt ggcgcgacaa gttgctgcga ttctcaccaa 4861 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgatacta 4921 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 4981 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 5041 tctgcgttta tatactagag caatacgcaa accgcctctc cccgcgcgtt ggccgattca 5101 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat 5161 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg 5221 tatgttgtgt ggaattgtga gcggataaca atttcacaca tactagagtc acacaggaaa 5281 gtactagatg ccttccgaat ctttacttga acgaatcacc cgtaaattac gtgacggatg 5341 gaaacgcctt atcgacatac ttaatcagcc aggataatac tagagccagg catcaaataa 5401 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 5461 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag 5521 agagagcctc gttgcgtttg tttgcacgaa ccatatgtaa gtatttcctt tactagagtc 5581 acacaggaaa gtactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc 5641 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag 5701 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg 5761 ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc 5821 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg 5881 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg 5941 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa 6001 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag 6061 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa 6121 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt 6181 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga 6241 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg 6301 aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 6361 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 6421 ggctcacctt cgggtgggcc tttctgcgtt tata //