BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K117007 1 BBa_K117007 colE7ImmE7-double terminator BBa_B0015 2008-10-07T11:00:00Z 2015-05-08T01:09:34Z [http://partsregistry.org/wiki/index.php?title=Part:BBa_K117001 Part BBa_K117001] was constructed by NTU iGEM 2008 team. [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 Part BBa_B0015] was obtained from the Registry This is an intermediate part obtained by ligating the double terminator BBa_B0015 behind colicin E7 (with Immunity) gene BBa_K117001. false false _209_ 0 2774 9 It's complicated false to be updated false Nguyen Xuan Hung component1979950 1 BBa_B0010 component1979949 1 BBa_K117001 component1979952 1 BBa_B0012 annotation1979952 1 BBa_B0012 range1979952 1 1996 2036 annotation1979949 1 BBa_K117001 range1979949 1 1 1899 annotation1979950 1 BBa_B0010 range1979950 1 1908 1987 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K117001 1 BBa_K117001 ColicinE7 with Immunity 2008-10-06T11:00:00Z 2015-05-08T01:09:34Z to be updated This gene sequence once expressed will produce colicin E7. This is an endonuclease with random cutting site and can kill various strains of EHEC bacteria. However, inside the host cell, immediatle after being produced, its toxicity is inhibited by Immunity protein (which is also expressed at the same time). These 2 proteins form a complex which is harmless to the host cell. Under SOS response, the host cell produces lysis protein, which cause the cell to lyze. At the same time, lysis protein remove Immunity protein from the complex with colE7. As a result, colE7 regains its toxicity and ability to kill the EHEC bacteria nearby. For information on lysis gene, please refer to our BBa_K117000 part. false false _209_ 0 2774 9 It's complicated true to be updated true Nguyen Xuan Hung annotation1989190 1 caeE7 toxicity domain range1989190 1 478 587 annotation1989189 1 caeE7 RBS range1989189 1 117 124 annotation1989191 1 Immunity (ceiE7) range1989191 1 1862 1899 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K117007_sequence 1 aatatccctccctgacttgacagagaaaataatggcgagtactttttgatctgtacataaaaccagtggttttatgtacagtattaactatgtaattaattgttttaacgcttaaaagagggaatttttatgagcggtggagatggacgtggccacaacagtggcgcacataacacaggtggtaacattaatggcggccctacggggcttggtggaaatggtggggcttctgacggctccggatggagttcggaaaataacccatggggtggcggttccggtagtggtgttcactggggaggtggctccggccatggcaatggcggggggaatagtaattccggtggtggcagcaattcatccgtagcagcacctatggcatttggttttcctgctttggctgctcctggtgccggaactcttggtatttccgtatctggtgaggctttatctgcggcaatagcggatatctttgcagccctgaaaggtccgtttaaattcagcgcatggggtattgcgctttacggcattctgccatctgaaatagcaaaagatgacccgaatatgatgtcaaagattgtgacgtcattaccggcagaaacagtgacgaatgttcaggtcagtacacttccgctggaccaggcaactgtcagcgtgacgaaacgtgtgacagatgttgtgaaggacacacgacagcatattgccgtagttgcaggtgtgcctatgagtgtgcctgttgtgaatgccaaaccaacacgtactccaggtgtatttcacgcatcatttcctggtgtcccttctctgacagtcagcactgtcaaaggccttccggtgtcaacaacccttccccgtggtattacggaggataaaggccggactgccgttcctgcgggatttacctttggtggtggttcacatgaagcggtgattcgctttccgaaagaaagtgggcagaagccggtttatgtatcagtgacagatgttcttaccccagcacaggtaaaacaacgtcaggatgaggaaaaacgcctccagcaggaatggaatgacgcacatccggtggaagtggctgaacgcaattatgaacaggcccgcgcagaactgaatcaggctaataaagatgttgccagaaaccaggaacgacaggctaaagctgttcaggtttataattctcgtaaaagtgaacttgatgcagcgaataaaactcttgctgatgcaaaggctgaaataaaacaattcgagcgatttgcccgagaaccaatggctgctggtcacagaatgtggcaaatggcagggcttaaggcccagcgggcacagacggatgtaaataataagaaggctgcatttgatgctgctgcaaaagaaaagtcagatgctgatgttgcattgagttctgcgttggagcgccgcaaacagaaagaaaataaagaaaaggacgctaaagccaaattagataaggagagtaaacggaataagccagggaaggcaacaggtaaaggaaaacctgtcaataataagtggttaaataatgcaggtaaagacttaggttctcctgttccagatcgtatagctaataaactacgtgataaggagtttaaaagtttcgatgattttcgtaagaaattctgggaagaagtgtcaaaagatcctgagttaagtaaacaatttagtaggaacaataatgatcgaatgaaggttggaaaagcgcccaagactagaacccaggatgtttcagggaagagaacttcattcgagcttcatcatgagaagccgatcagccaaaatggtggtgtctatgatatggataacatcagcgtggtaacacctaaaaggcatattgatattcaccgaggtaaataatatggaactgaaaaatagtattagtgattacacagaggctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K117001_sequence 1 aatatccctccctgacttgacagagaaaataatggcgagtactttttgatctgtacataaaaccagtggttttatgtacagtattaactatgtaattaattgttttaacgcttaaaagagggaatttttatgagcggtggagatggacgtggccacaacagtggcgcacataacacaggtggtaacattaatggcggccctacggggcttggtggaaatggtggggcttctgacggctccggatggagttcggaaaataacccatggggtggcggttccggtagtggtgttcactggggaggtggctccggccatggcaatggcggggggaatagtaattccggtggtggcagcaattcatccgtagcagcacctatggcatttggttttcctgctttggctgctcctggtgccggaactcttggtatttccgtatctggtgaggctttatctgcggcaatagcggatatctttgcagccctgaaaggtccgtttaaattcagcgcatggggtattgcgctttacggcattctgccatctgaaatagcaaaagatgacccgaatatgatgtcaaagattgtgacgtcattaccggcagaaacagtgacgaatgttcaggtcagtacacttccgctggaccaggcaactgtcagcgtgacgaaacgtgtgacagatgttgtgaaggacacacgacagcatattgccgtagttgcaggtgtgcctatgagtgtgcctgttgtgaatgccaaaccaacacgtactccaggtgtatttcacgcatcatttcctggtgtcccttctctgacagtcagcactgtcaaaggccttccggtgtcaacaacccttccccgtggtattacggaggataaaggccggactgccgttcctgcgggatttacctttggtggtggttcacatgaagcggtgattcgctttccgaaagaaagtgggcagaagccggtttatgtatcagtgacagatgttcttaccccagcacaggtaaaacaacgtcaggatgaggaaaaacgcctccagcaggaatggaatgacgcacatccggtggaagtggctgaacgcaattatgaacaggcccgcgcagaactgaatcaggctaataaagatgttgccagaaaccaggaacgacaggctaaagctgttcaggtttataattctcgtaaaagtgaacttgatgcagcgaataaaactcttgctgatgcaaaggctgaaataaaacaattcgagcgatttgcccgagaaccaatggctgctggtcacagaatgtggcaaatggcagggcttaaggcccagcgggcacagacggatgtaaataataagaaggctgcatttgatgctgctgcaaaagaaaagtcagatgctgatgttgcattgagttctgcgttggagcgccgcaaacagaaagaaaataaagaaaaggacgctaaagccaaattagataaggagagtaaacggaataagccagggaaggcaacaggtaaaggaaaacctgtcaataataagtggttaaataatgcaggtaaagacttaggttctcctgttccagatcgtatagctaataaactacgtgataaggagtttaaaagtttcgatgattttcgtaagaaattctgggaagaagtgtcaaaagatcctgagttaagtaaacaatttagtaggaacaataatgatcgaatgaaggttggaaaagcgcccaagactagaacccaggatgtttcagggaagagaacttcattcgagcttcatcatgagaagccgatcagccaaaatggtggtgtctatgatatggataacatcagcgtggtaacacctaaaaggcatattgatattcaccgaggtaaataatatggaactgaaaaatagtattagtgattacacagaggc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z