LOCUS       BBa_K117009             2264 bp    DNA     linear       01-Jun-2024
DEFINITION  colicin E7 production system induced by lactose
ACCESSION   BBa_K117009
VERSION     BBa_K117009.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     RBS             345..352
                     /label=caeE7 RBS
     misc_feature    706..815
                     /label=caeE7 toxicity domain
     CDS             2090..2127
                     /label=Immunity (ceiE7)
     misc_feature    229..2127
                     /label=BBa_K117001
     stem_loop       2147..2190
                     /label=stem_loop
     misc_feature    2136..2215
                     /label=BBa_B0010
     terminator      2136..2215
                     /label=BBa_B0010
     stem_loop       2231..2250
                     /label=T7 TE
     misc_feature    2257..2257
                     /label=stop
     polyA_site      2251..2264
                     /label=polya
     misc_feature    2224..2264
                     /label=BBa_B0012
     terminator      2224..2264
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagaa tatccctccc
      241 tgacttgaca gagaaaataa tggcgagtac tttttgatct gtacataaaa ccagtggttt
      301 tatgtacagt attaactatg taattaattg ttttaacgct taaaagaggg aatttttatg
      361 agcggtggag atggacgtgg ccacaacagt ggcgcacata acacaggtgg taacattaat
      421 ggcggcccta cggggcttgg tggaaatggt ggggcttctg acggctccgg atggagttcg
      481 gaaaataacc catggggtgg cggttccggt agtggtgttc actggggagg tggctccggc
      541 catggcaatg gcggggggaa tagtaattcc ggtggtggca gcaattcatc cgtagcagca
      601 cctatggcat ttggttttcc tgctttggct gctcctggtg ccggaactct tggtatttcc
      661 gtatctggtg aggctttatc tgcggcaata gcggatatct ttgcagccct gaaaggtccg
      721 tttaaattca gcgcatgggg tattgcgctt tacggcattc tgccatctga aatagcaaaa
      781 gatgacccga atatgatgtc aaagattgtg acgtcattac cggcagaaac agtgacgaat
      841 gttcaggtca gtacacttcc gctggaccag gcaactgtca gcgtgacgaa acgtgtgaca
      901 gatgttgtga aggacacacg acagcatatt gccgtagttg caggtgtgcc tatgagtgtg
      961 cctgttgtga atgccaaacc aacacgtact ccaggtgtat ttcacgcatc atttcctggt
     1021 gtcccttctc tgacagtcag cactgtcaaa ggccttccgg tgtcaacaac ccttccccgt
     1081 ggtattacgg aggataaagg ccggactgcc gttcctgcgg gatttacctt tggtggtggt
     1141 tcacatgaag cggtgattcg ctttccgaaa gaaagtgggc agaagccggt ttatgtatca
     1201 gtgacagatg ttcttacccc agcacaggta aaacaacgtc aggatgagga aaaacgcctc
     1261 cagcaggaat ggaatgacgc acatccggtg gaagtggctg aacgcaatta tgaacaggcc
     1321 cgcgcagaac tgaatcaggc taataaagat gttgccagaa accaggaacg acaggctaaa
     1381 gctgttcagg tttataattc tcgtaaaagt gaacttgatg cagcgaataa aactcttgct
     1441 gatgcaaagg ctgaaataaa acaattcgag cgatttgccc gagaaccaat ggctgctggt
     1501 cacagaatgt ggcaaatggc agggcttaag gcccagcggg cacagacgga tgtaaataat
     1561 aagaaggctg catttgatgc tgctgcaaaa gaaaagtcag atgctgatgt tgcattgagt
     1621 tctgcgttgg agcgccgcaa acagaaagaa aataaagaaa aggacgctaa agccaaatta
     1681 gataaggaga gtaaacggaa taagccaggg aaggcaacag gtaaaggaaa acctgtcaat
     1741 aataagtggt taaataatgc aggtaaagac ttaggttctc ctgttccaga tcgtatagct
     1801 aataaactac gtgataagga gtttaaaagt ttcgatgatt ttcgtaagaa attctgggaa
     1861 gaagtgtcaa aagatcctga gttaagtaaa caatttagta ggaacaataa tgatcgaatg
     1921 aaggttggaa aagcgcccaa gactagaacc caggatgttt cagggaagag aacttcattc
     1981 gagcttcatc atgagaagcc gatcagccaa aatggtggtg tctatgatat ggataacatc
     2041 agcgtggtaa cacctaaaag gcatattgat attcaccgag gtaaataata tggaactgaa
     2101 aaatagtatt agtgattaca cagaggctac tagagccagg catcaaataa aacgaaaggc
     2161 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     2221 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
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