BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K117001 1 BBa_K117001 ColicinE7 with Immunity 2008-10-06T11:00:00Z 2015-05-08T01:09:34Z to be updated This gene sequence once expressed will produce colicin E7. This is an endonuclease with random cutting site and can kill various strains of EHEC bacteria. However, inside the host cell, immediatle after being produced, its toxicity is inhibited by Immunity protein (which is also expressed at the same time). These 2 proteins form a complex which is harmless to the host cell. Under SOS response, the host cell produces lysis protein, which cause the cell to lyze. At the same time, lysis protein remove Immunity protein from the complex with colE7. As a result, colE7 regains its toxicity and ability to kill the EHEC bacteria nearby. For information on lysis gene, please refer to our BBa_K117000 part. false false _209_ 0 2774 9 It's complicated true to be updated true Nguyen Xuan Hung annotation1989191 1 Immunity (ceiE7) range1989191 1 1862 1899 annotation1989189 1 caeE7 RBS range1989189 1 117 124 annotation1989190 1 caeE7 toxicity domain range1989190 1 478 587 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961224 1 -35 range1961224 1 137 142 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961225 1 -10 range1961225 1 161 166 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961227 1 start range1961227 1 173 173 BBa_K117009 1 BBa_K117009 colicin E7 production system induced by lactose 2008-10-07T11:00:00Z 2015-05-08T01:09:34Z Both subparts: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K117005 BBa_K117005] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K117007 BBa_K117007] were constructed by NTU iGEM 2008 team. This system is induced by lactose to produce colicin E7 with Immunity protein. false false _209_ 0 2774 9 It's complicated true to be updated true Nguyen Xuan Hung component1979982 1 BBa_B0034 component1979986 1 BBa_K117001 component1979987 1 BBa_B0010 component1979989 1 BBa_B0012 component1979974 1 BBa_R0010 annotation1979989 1 BBa_B0012 range1979989 1 2224 2264 annotation1979987 1 BBa_B0010 range1979987 1 2136 2215 annotation1979982 1 BBa_B0034 range1979982 1 209 220 annotation1979986 1 BBa_K117001 range1979986 1 229 2127 annotation1979974 1 BBa_R0010 range1979974 1 1 200 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K117009_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagaatatccctccctgacttgacagagaaaataatggcgagtactttttgatctgtacataaaaccagtggttttatgtacagtattaactatgtaattaattgttttaacgcttaaaagagggaatttttatgagcggtggagatggacgtggccacaacagtggcgcacataacacaggtggtaacattaatggcggccctacggggcttggtggaaatggtggggcttctgacggctccggatggagttcggaaaataacccatggggtggcggttccggtagtggtgttcactggggaggtggctccggccatggcaatggcggggggaatagtaattccggtggtggcagcaattcatccgtagcagcacctatggcatttggttttcctgctttggctgctcctggtgccggaactcttggtatttccgtatctggtgaggctttatctgcggcaatagcggatatctttgcagccctgaaaggtccgtttaaattcagcgcatggggtattgcgctttacggcattctgccatctgaaatagcaaaagatgacccgaatatgatgtcaaagattgtgacgtcattaccggcagaaacagtgacgaatgttcaggtcagtacacttccgctggaccaggcaactgtcagcgtgacgaaacgtgtgacagatgttgtgaaggacacacgacagcatattgccgtagttgcaggtgtgcctatgagtgtgcctgttgtgaatgccaaaccaacacgtactccaggtgtatttcacgcatcatttcctggtgtcccttctctgacagtcagcactgtcaaaggccttccggtgtcaacaacccttccccgtggtattacggaggataaaggccggactgccgttcctgcgggatttacctttggtggtggttcacatgaagcggtgattcgctttccgaaagaaagtgggcagaagccggtttatgtatcagtgacagatgttcttaccccagcacaggtaaaacaacgtcaggatgaggaaaaacgcctccagcaggaatggaatgacgcacatccggtggaagtggctgaacgcaattatgaacaggcccgcgcagaactgaatcaggctaataaagatgttgccagaaaccaggaacgacaggctaaagctgttcaggtttataattctcgtaaaagtgaacttgatgcagcgaataaaactcttgctgatgcaaaggctgaaataaaacaattcgagcgatttgcccgagaaccaatggctgctggtcacagaatgtggcaaatggcagggcttaaggcccagcgggcacagacggatgtaaataataagaaggctgcatttgatgctgctgcaaaagaaaagtcagatgctgatgttgcattgagttctgcgttggagcgccgcaaacagaaagaaaataaagaaaaggacgctaaagccaaattagataaggagagtaaacggaataagccagggaaggcaacaggtaaaggaaaacctgtcaataataagtggttaaataatgcaggtaaagacttaggttctcctgttccagatcgtatagctaataaactacgtgataaggagtttaaaagtttcgatgattttcgtaagaaattctgggaagaagtgtcaaaagatcctgagttaagtaaacaatttagtaggaacaataatgatcgaatgaaggttggaaaagcgcccaagactagaacccaggatgtttcagggaagagaacttcattcgagcttcatcatgagaagccgatcagccaaaatggtggtgtctatgatatggataacatcagcgtggtaacacctaaaaggcatattgatattcaccgaggtaaataatatggaactgaaaaatagtattagtgattacacagaggctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_B0034_sequence 1 aaagaggagaaa BBa_K117001_sequence 1 aatatccctccctgacttgacagagaaaataatggcgagtactttttgatctgtacataaaaccagtggttttatgtacagtattaactatgtaattaattgttttaacgcttaaaagagggaatttttatgagcggtggagatggacgtggccacaacagtggcgcacataacacaggtggtaacattaatggcggccctacggggcttggtggaaatggtggggcttctgacggctccggatggagttcggaaaataacccatggggtggcggttccggtagtggtgttcactggggaggtggctccggccatggcaatggcggggggaatagtaattccggtggtggcagcaattcatccgtagcagcacctatggcatttggttttcctgctttggctgctcctggtgccggaactcttggtatttccgtatctggtgaggctttatctgcggcaatagcggatatctttgcagccctgaaaggtccgtttaaattcagcgcatggggtattgcgctttacggcattctgccatctgaaatagcaaaagatgacccgaatatgatgtcaaagattgtgacgtcattaccggcagaaacagtgacgaatgttcaggtcagtacacttccgctggaccaggcaactgtcagcgtgacgaaacgtgtgacagatgttgtgaaggacacacgacagcatattgccgtagttgcaggtgtgcctatgagtgtgcctgttgtgaatgccaaaccaacacgtactccaggtgtatttcacgcatcatttcctggtgtcccttctctgacagtcagcactgtcaaaggccttccggtgtcaacaacccttccccgtggtattacggaggataaaggccggactgccgttcctgcgggatttacctttggtggtggttcacatgaagcggtgattcgctttccgaaagaaagtgggcagaagccggtttatgtatcagtgacagatgttcttaccccagcacaggtaaaacaacgtcaggatgaggaaaaacgcctccagcaggaatggaatgacgcacatccggtggaagtggctgaacgcaattatgaacaggcccgcgcagaactgaatcaggctaataaagatgttgccagaaaccaggaacgacaggctaaagctgttcaggtttataattctcgtaaaagtgaacttgatgcagcgaataaaactcttgctgatgcaaaggctgaaataaaacaattcgagcgatttgcccgagaaccaatggctgctggtcacagaatgtggcaaatggcagggcttaaggcccagcgggcacagacggatgtaaataataagaaggctgcatttgatgctgctgcaaaagaaaagtcagatgctgatgttgcattgagttctgcgttggagcgccgcaaacagaaagaaaataaagaaaaggacgctaaagccaaattagataaggagagtaaacggaataagccagggaaggcaacaggtaaaggaaaacctgtcaataataagtggttaaataatgcaggtaaagacttaggttctcctgttccagatcgtatagctaataaactacgtgataaggagtttaaaagtttcgatgattttcgtaagaaattctgggaagaagtgtcaaaagatcctgagttaagtaaacaatttagtaggaacaataatgatcgaatgaaggttggaaaagcgcccaagactagaacccaggatgtttcagggaagagaacttcattcgagcttcatcatgagaagccgatcagccaaaatggtggtgtctatgatatggataacatcagcgtggtaacacctaaaaggcatattgatattcaccgaggtaaataatatggaactgaaaaatagtattagtgattacacagaggc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z