LOCUS       BBa_K1172204            1330 bp    DNA     linear       22-Dec-2024
DEFINITION  GldA - glycerol dehydrogenase from Escherichia coli with P lacI 
            and RBS
ACCESSION   BBa_K1172204
VERSION     BBa_K1172204.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..1330
                     /label=gldA
     misc_feature    227..229
                     /label=start
     misc_feature    1328..1330
                     /label=stop
     CDS             227..1330
                     /label=BBa_K1172201
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg accgcattat
      241 tcaatcaccg ggtaaataca tccagggcgc tgatgtgatt aatcgtctgg gcgaatacct
      301 gaagccgctg gcagaacgct ggttagtggt gggtgacaaa tttgttttag gttttgctca
      361 atccactgtc gagaaaagct ttaaagatgc tggactggta gtagaaattg cgccgtttgg
      421 cggtgaatgt tcgcaaaatg agatcgaccg tctgcgtggc atcgcggaga ctgcgcagtg
      481 tggcgcaatt ctcggtatcg gtggcggaaa aaccctcgat actgccaaag cactggcaca
      541 tttcatgggt gttccggtag cgatcgcacc gactatcgcc tctaccgatg caccgtgcag
      601 cgcattgtct gttatctaca ccgatgaggg tgagtttgac cgctatctgc tgttgccaaa
      661 taacccgaat atggtcattg tcgacaccaa aatcgtcgct ggcgcacctg cacgtctgtt
      721 agcggcgggt atcggcgatg cgctggcaac ctggtttgaa gcgcgtgcct gctctcgtag
      781 cggcgcgacc accatggcgg gcggcaagtg cacccaggct gcgctggcac tggctgaact
      841 gtgctacaac accctgctgg aagaaggcga aaaagcgatg cttgctgccg aacagcatgt
      901 agtgactccg gcgctggagc gcgtgattga agcgaacacc tatttgagcg gtgttggttt
      961 tgaaagtggt ggtctggctg cggcgcacgc agtgcataac ggcctgaccg ctatcccgga
     1021 cgcgcatcac tattatcacg gtgaaaaagt ggcattcggt acgctgacgc agctggttct
     1081 ggaaaatgcg ccggtggagg aaatcgaaac cgtagctgcc cttagccatg cggtaggttt
     1141 gccaataact ctcgctcaac tggatattaa agaagatgtc ccggcgaaaa tgcgaattgt
     1201 ggcagaagcg gcatgtgcag aaggtgaaac cattcacaac atgcctggcg gcgcgacgcc
     1261 agatcaggtt tacgccgctc tgctggtagc cgaccagtac ggtcagcgtt tcctgcaaga
     1321 gtgggaataa
//