LOCUS BBa_K1172304 3635 bp DNA linear 25-Nov-2024 DEFINITION Riboflavin synthesis gene cluster from s. oneidensis under control of T7 promoter and strong RBS ACCESSION BBa_K1172304 VERSION BBa_K1172304.1 FEATURES Location/Qualifiers misc_feature 21..32 /label=B0034 promoter 1..20 /label=T7 promoter RBS 24..27 /label=RBS promoter 1..32 /label=BBa_K525998 CDS 39..1184 /label=ribD CDS 1192..1848 /label=SO_3468 CDS 1915..3018 /label=ribBA CDS 3159..3635 /label=ribE misc_feature 272..277 /label=Pst1 C->T misc_feature 1682..1687 /label=Pst1 C->T misc_feature 1878..1883 /label=EcoR1 C->T misc_feature 39..41 /label=atg misc_feature 1192..1194 /label=atg misc_feature 1915..1917 /label=atg misc_feature 3159..3161 /label=atg misc_feature 1182..1184 /label=tga misc_feature 1846..1848 /label=tag misc_feature 3016..3018 /label=taa misc_feature 3633..3635 /label=taa CDS 39..3635 /label=BBa_K1172303 ORIGIN 1 taatacgact cactataggg aaagaggaga aatactagat gaattggtca gtactcgata 61 accaaatgat gagccgagct attcaattgg ctcgcaaagg attttatacc actcgcccca 121 atcccagtgt gggctgcgtt atcgttaacg ataaccagat tgtcggtgaa ggttatcatc 181 aaaaagccgg agagccccac gctgaagtgc atgcgctgcg catggctggc gagcttgccc 241 gtggcgcgac tgcttatgtc accttagaac cctgtagcca ttatggtcgt acgccgccct 301 gcgccttggc gctgatcaat attggtgtaa agcgggtggt ggtggcggtt gaagatccta 361 atccgcaggt cggcgggcgc ggtattcaaa tgctgcgcga tgcgggtatt caagtggatg 421 tgggcttgca tcgtgatgag gcctatgcct taaatcttgg ttttatgaag cgcatggagt 481 cgggtctacc ttgggtgacg gtaaagcttg ccgcgagtct tgatggtaaa actgcgttat 541 caaacggggt gtctaaatgg atcactggcc ctgaagctcg ccgcgatgtg cagcgcttaa 601 gattacgatc ctgcgcgctg atcacgggga ttgaaacggt actggccgat gatccatcgc 661 tcaatgtgcg ctactcagag cttggcagtt taagagcgca attgagtgag tcgcaggttt 721 tacagccgct gcgagtggtt ttggacagtc gcgcccgcat gcctatcacg gcagcattgt 781 ttgcgattga atcacccatt ttattagtct cgacagtgag ctattcgtca gactttatgg 841 tgcaattacc cgcccatgtg acttgcctcc aattaccagc cattgagggt cgaatctcct 901 tgtctgcact attaagctat ttaggcaaaa gctgtaatca ggtgcttatc gaagcgggcg 961 cgactttagc tggcgccttt atcggctcag gattagccga tgaattagtg ctttatcaag 1021 cgatgaaaat ccttggcggc caaggacgta atctacttga attacccgat tatcaaatga 1081 tggccgatat tccgaccctc aaattggtcg acgagcgtaa agtgggcgcg gatacgcgct 1141 tcaccttgag gctcgagtcc aatccatctt tagcgaataa gtgagttaac catgtttact 1201 gggattattg aggccgttgg tacactgcga aagcttgagc gtaaaggcga tgatattcgc 1261 ttaacggtcg ccagtggcaa actggattta accgatgtgc gtttaggcga cagtatcgcc 1321 accaacggtg tatgtttgac cgtggttcaa cagttagccg atggctatgt ggctgatgtg 1381 tcagctgaaa ccgtcagcct tacaggtttt gcaaactata aagtgggtac taaggtcaat 1441 cttgaaaagg ccgtcacccc aactactcgt ttaggcggcc atatggtgag tggtcatgtg 1501 gatgggattg ctactgttga gcagcgtctt gctcgtggtc aagcaatcga attttggtta 1561 gcggctccca cggagcttgc tcgctatatt gcccataaag gttctatcac gattgatggc 1621 gtgagcctga cagtaaacga agtcgatgga caccgttttc gattaacgat tgtgccccat 1681 actgtaggtg aaaccacgtt ggtggattta aaagcaggcg ataaggtcaa tattgaagtg 1741 gacttaattg cccgttattt agagcgttta atgcgttttg acagcaaaga aacccaaggc 1801 ggaggggtga ccatggaaat gttagcccgt gctggctttg tgcgttagta cactggcact 1861 cagttcactt aggtatagaa tttataacaa cagtcacatt ataaaggtcc tacaatggcg 1921 ctgcacagta tagaagagat catcgaggat attcgtcaag gcaaaatggt tattttgatg 1981 gatgacgagg acagagaaaa cgaaggcgat ctgattttgg cggccgaaat ggtgacgcca 2041 gaagcgatta actttatggc gaaatacggc cgcgggttaa tctgccaaac gatgaccaag 2101 gcccgttgcc agcagttaaa tctgcccttg atggtaacca ataacaatgc gcagttctcg 2161 accaacttta cggtatcgat tgaagctgcc gaaggcgtaa ccacagggat ttcggcccac 2221 gatcgcgctg taaccgttaa agcggccgta gcaaaagatg ctaaagcgtc ggacttagtt 2281 cagcctgggc atatcttccc gttaatggcg caggatggtg gcgtattaac ccgcgcaggt 2341 cacactgaag cgggctgtga tttagcccgt ttagcagggc ttgaaccatc gggggtgatt 2401 gttgaaattt tgaacgaaga cggcactatg gcccgtcgcc cagatttaga gattttctct 2461 gaaaaacatg gcattaaaat cggcactatt gcggccctga ttgaataccg taataccaaa 2521 gaaacgaccg ttgtgcgtga agccaaatgc aaactgccaa cccgtttcgg tgagttcgat 2581 atggtgacct ttagggatac tatcgacaat caattgcact ttgccttagt gaaaggcgag 2641 gtgaagcctg actgtttagt acgggtacat ttacagaata cctttaacga tctacttcac 2701 tctgagcgtg atcagcaacg tagctggccg ctcgaaaaag cgatggcgcg gatttctgcc 2761 gaaggtggtg tattagtact gcttggcaat caggaacatt ccagtgaaat cctcgccaaa 2821 gtcaaagcct ttgaagcgga agatcaaggt cagaccccag tatcggctaa atggcaaggc 2881 acctcacgcc gtgtaggtgt gggttcgcaa atcctcgcca gtttaggcgt gacaaaaatg 2941 cgactcttaa gctcgcccaa acgttatcac tcactttcag gctttggcct tgaagtgacc 3001 gaatgcgtag ctgagtaaca ggttaagctt gtaataggtt gcactaaggt aaaattaacc 3061 atttctatca tttgcataag gccgcgggca attgactgtg gtatcatgtc gccacttttc 3121 gcccagagct gggtgcttta gctaaattag gtaagaaaat gaacgtagtt caaggtaata 3181 tcgaagcgaa gaatgccaaa gttgcgattg taatttcgcg ttttaacagc tttttagtcg 3241 aaagcctgct tgaaggtgca cttgacacgc tgaaacgttt tggccaagtc agtgatgaaa 3301 acatcactgt tgtccgtgta cctggtgcgg ttgagttacc gctggctgca cgtcgtgttg 3361 ccgcaagtgg taagtttgac ggtattattg cacttggtgc ggtaatccgt ggtggtaccc 3421 ctcactttga ttttgttgca ggcgaatgta acaaaggtct ggctcaaatt gcattagagt 3481 tcgatctgcc cgttgctttc ggtgtattga ctacagatac cattgaacaa gctattgagc 3541 gttcaggtac taaagcgggt aacaaaggcg gcgaagctgc tctaagcttg cttgaaatgg 3601 tcaacgttct gcaacagctt gaacaacagt tgtaa //