LOCUS BBa_K1172402 5338 bp DNA linear 25-Nov-2024 DEFINITION mtrCAB gene cluster with IPTG inducible lac promoter and RBS ACCESSION BBa_K1172402 VERSION BBa_K1172402.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 misc_feature 1..220 /label=BBa_J04500 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga tgaacgcaca 241 aaaatcaaaa atcgcactgc tgctcgcagc aagtgccgtc acaatggcct taaccggctg 301 tggtggaagc gatggtaata acggcaatga tggtagtgat ggtggtgagc cagcaggtag 361 catccagacg ttaaacctag atatcactaa agtaagctat gaaaatggtg cacctatggt 421 cactgttttc gccactaacg aagccgacat gccagtgatt ggtctcgcaa atttagaaat 481 caaaaaagca ctgcaattaa taccggaagg ggcgacaggc ccaggtaata gcgctaactg 541 gcaaggctta ggctcatcaa agagctatgt cgataataaa aacggtagct atacctttaa 601 attcgacgcc ttcgatagta ataaggtctt taatgctcaa ttaacgcaac gctttaacgt 661 tgtttctgct gcgggtaaat tagcagacgg aacgaccgtt cccgttgccg aaatggttga 721 agatttcgac ggccaaggta atgcgccgca atatacaaaa aatatcgtta gccacgaagt 781 atgtgcttct tgccacgtag aaggtgaaaa gatttatcac caagctactg aagtcgaaac 841 ttgtatttct tgccacactc aagagtttgc ggatggtcgc ggcaaacccc atgtcgcctt 901 tagtcactta attcacaatg tgcataatgc caacaaagct tggggcaaag acaataaaat 961 ccctacagtt gcacaaaata ttgtccaaga taattgccaa gtttgtcacg ttgaatccga 1021 catgctcacc gaggcaaaaa actggtcacg tattccaaca atggaagtct gttctagctg 1081 tcacgtagac atcgattttg ctgcgggtaa aggccactct caacaactcg ataactccaa 1141 ctgtatcgcc tgccataaca gcgactggac tgctgagtta cacacagcca aaaccaccgc 1201 aactaagaac ttgattaatc aatacggtat cgagactacc tcgacaatta ataccgaaac 1261 taaagcagcc acaattagtg ttcaagttgt agatgcgaac ggtactgctg ttgatctcaa 1321 gaccatcctg cctaaagtgc aacgcttaga gatcatcacc aacgttggtc ctaataatgc 1381 aaccttaggt tatagtggca aagattcaat atttgcaatc aaaaatggag ctcttgatcc 1441 aaaagctact atcaatgatg ctggcaaact ggtttatacc actactaaag acctcaaact 1501 tggccaaaac ggcgcagaca gcgacacagc atttagcttt gtaggttggt caatgtgttc 1561 tagcgaaggt aagtttgtag actgtgcaga ccctgcattt gatggtgttg atgtaactaa 1621 gtataccggc atgaaagcgg atttagcctt tgctactttg tcaggtaaag caccaagtac 1681 tcgccacgtt gattctgtta acatgacagc ctgtgccaat tgccacactg ctgagttcga 1741 aattcacaaa ggcaaacaac atgcaggctt tgtgatgaca gagcaactat cacacaccca 1801 agatgctaac ggtaaagcga ttgtaggcct tgacgcatgt gtgacttgtc atactcctga 1861 tggcacctat agctttgcca accgtggtgc gctagagcta aaactacaca aaaaacacgt 1921 tgaagatgcc tacggcctca ttggtggcaa ttgtgcctct tgtcactcag acttcaacct 1981 tgagtctttc aagaagaaag gcgcattgaa tactgccgct gcaacagata aaacaggtct 2041 atattctacg ccgatcactg caacttgtac tacctgtcac acagttggca gccagtacat 2101 ggtccatacg aaagaaaccc tggagtcttt cggtgcagtt gttgatggca caaaagatga 2161 tgctaccagt gcggcacagt cagaaacctg tttctactgc cataccccaa cagttgcaga 2221 tcacactaaa gtgaaaatgt aaatgaagaa ctgcctaaaa atgaaaaacc tactgccggc 2281 acttaccatc acaatggcaa tgtctgcaat tatggcatta gtcgtcacac caaacgctta 2341 tgcgtcgaag tgggatgaga aaatgacgcc agagcaagtc gaagccacct tagataagaa 2401 gtttgccgaa ggcaactact cccctaaagg cgccgattct tgcttgatgt gccataagaa 2461 atccgaaaaa gtcatggacc ttttcaaagg tgtccacggt gcgattgact cctctaagag 2521 tccaatggct ggcctgcaat gtgaggcatg ccacggccca ctgggtcagc acaacaaagg 2581 cggcaacgag ccgatgatca cttttggtaa gcaatcaacc ttaagtgccg acaagcaaaa 2641 cagcgtatgt atgagctgtc accaagacga taagcgtatg tcttggaatg gcggtcacca 2701 tgacaatgcc gatgttgctt gtgcttcttg tcaccaagta cacgtcgcaa aagatcctgt 2761 gttatctaaa aacacggaaa tggaagtctg tactagctgc catacaaagc aaaaagcgga 2821 tatgaataaa cgctcaagtc acccactcaa atgggcacaa atgacctgta gcgactgtca 2881 caatccccat gggagcatga cagattccga tcttaacaag cctagcgtga atgatacctg 2941 ttattcctgt cacgccgaaa aacgcggccc aaaactttgg gagcatgcac ccgtcactga 3001 gaattgtgtc acttgccaca atcctcacgg tagtgtgaat gacggtatgc tgaaaacccg 3061 tgcgccacag ctatgtcagc aatgtcacgc cagcgatggc cacgccagca acgcctactt 3121 aggtaacact ggattaggtt caaatgtcgg tgacaatgcc tttactggtg gaagaagctg 3181 cttaaattgc catagtcagg ttcatggttc taaccatcca tctggcaagc tattacagcg 3241 ctaaatgaaa tttaaactca atttgatcac tctagcgtta ttagccaaca caggcttggc 3301 cgtcgctgct gatggttatg gtctagcgaa tgccaatact gaaaaagtga aattatccgc 3361 atggagctgt aaaggctgcg tcgttgaaac gggcacatca ggcactgtgg gtgtcggtgt 3421 cggttataac agcgaagagg atattcgctc tgccaatgcc tttggtacat ccaatgaagt 3481 ggcgggtaaa tttgatgccg atttaaactt taaaggtgaa aagggttatc gtgccagtgt 3541 tgatgcttat caactcggta tggatggcgg tcgcttagat gtcaatgcgg gcaaacaagg 3601 ccagtacaac gtcaatgtga actatcgcca aattgctacc tacgacagca atagcgccct 3661 atcgccctac gcgggtattg gtggcaataa cctcacgtta ccggataact ggataacagc 3721 aggttcaagc aaccaaatgc cactcttgat ggacagcctc aatgccctcg aactctcact 3781 taaacgtgag cgcacggggt tgggatttga atatcaaggt gaatccctgt ggagcaccta 3841 tgttaactac atgcgtgaag agaaaaccgg cttaaaacaa gcctctggta gcttcttcaa 3901 ccaatcgatg atgttagcag agccggtgga ttacaccact gacaccattg aagcgggtgt 3961 caaactcaag ggtgatcgtt ggtttaccgc actcagttac aatgggtcaa tattcaaaaa 4021 cgaatacaac caattggact ttgaaaatgc ttttaacccc acctttggtg ctcaaaccca 4081 aggtacgatg gcactcgatc cggataacca gtcacacacc gtgtcgctga tgggacagta 4141 caacgatggc agcaacgcac tgtcgggtcg tattctgacc ggacaaatga gccaagatca 4201 ggcgttagtg acggataact accgttatgc taatcagctc aataccgatg ccgtcgatgc 4261 caaagtcgat ctactgggta tgaacctgaa agtcgttagc aaagtgagca atgatcttcg 4321 cttaacaggt agttacgatt attacgaccg tgacaataat acccaagtag aagaatggac 4381 tcagatcagc atcaacaatg tcaacggtaa ggtggcttat aacacccctt acgataatcg 4441 tacgcaacgc tttaaagttg ccgcagatta tcgcattacc cgcgatatca aactcgatgg 4501 tggttatgac ttcaaacgtg accaacgtga ttatcaagac cgtgaaacca cggatgaaaa 4561 taccgtttgg gcccgtttac gtgtaaacag cttcgatact tgggacatgt gggtaaaagg 4621 cagttacggt aaccgtgacg gctcacaata ccaagcgtct gaatggacct cttctgaaac 4681 caacagcctg ttacgtaagt acaatctggc tgaccgtgac agaactcaag tcgaagcacg 4741 gatcacccat tcgccattag aaagcctgac tatcgatgtt ggtgcccgtt acgcgttaga 4801 tgattatacc gatactgtga ttggattaac tgagtcaaaa gacaccagtt atgatgccaa 4861 catcagttat atgatcaccg ctgacttact ggcaaccgcc ttctacaatt accaaaccat 4921 tgagtctgaa caggcgggta gcagcaatta cagcacccca acgtggacag gctttataga 4981 agatcaggta gatgtggtcg gtgcaggtat cagctacaac aatctgctgg agaacaagtt 5041 acgcctagga ctggactaca cctattccaa ctccgacagt aacactcaag tcagacaagg 5101 tatcactggc gactatggtg attattttgc caaagtgcat aacattaact tatacgctca 5161 atatcaagcc accgagaaac tcgcgctgcg cttcgattac aaaattgaga actataagga 5221 caatgacgcc gcaaatgata tcgccgttga tggcatttgg aacgtcgtag gttttggtag 5281 taacagccat gactacaccg cacaaatgct gatgctgagc atgagttaca aactctaa //