LOCUS BBa_K1172403 5174 bp DNA linear 26-Nov-2024 DEFINITION mtrCAB with medium Promoter and medium RBS ACCESSION BBa_K1172403 VERSION BBa_K1172403.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23110 RBS 44..56 /label=RBS-3\Weak RBS 50..53 /label=RBS misc_feature 44..56 /label=BBa_B0032 RBS 44..56 /label=BBa_B0032 misc_feature 1..56 /label=BBa_K608006 CDS 63..2078 /label=mtrC misc_feature 63..68 /label=start misc_feature 1860..1860 /label=PstI site G->A CDS 2079..3080 /label=mtrA misc_feature 2145..2145 /label=pstI site G->A CDS 3081..5174 /label=mtrB misc_feature 2079..2081 /label=start misc_feature 3081..3083 /label=start CDS 63..5174 /label=BBa_K1172401 ORIGIN 1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagtcacaca ggaaagtact 61 agatgatgaa cgcacaaaaa tcaaaaatcg cactgctgct cgcagcaagt gccgtcacaa 121 tggccttaac cggctgtggt ggaagcgatg gtaataacgg caatgatggt agtgatggtg 181 gtgagccagc aggtagcatc cagacgttaa acctagatat cactaaagta agctatgaaa 241 atggtgcacc tatggtcact gttttcgcca ctaacgaagc cgacatgcca gtgattggtc 301 tcgcaaattt agaaatcaaa aaagcactgc aattaatacc ggaaggggcg acaggcccag 361 gtaatagcgc taactggcaa ggcttaggct catcaaagag ctatgtcgat aataaaaacg 421 gtagctatac ctttaaattc gacgccttcg atagtaataa ggtctttaat gctcaattaa 481 cgcaacgctt taacgttgtt tctgctgcgg gtaaattagc agacggaacg accgttcccg 541 ttgccgaaat ggttgaagat ttcgacggcc aaggtaatgc gccgcaatat acaaaaaata 601 tcgttagcca cgaagtatgt gcttcttgcc acgtagaagg tgaaaagatt tatcaccaag 661 ctactgaagt cgaaacttgt atttcttgcc acactcaaga gtttgcggat ggtcgcggca 721 aaccccatgt cgcctttagt cacttaattc acaatgtgca taatgccaac aaagcttggg 781 gcaaagacaa taaaatccct acagttgcac aaaatattgt ccaagataat tgccaagttt 841 gtcacgttga atccgacatg ctcaccgagg caaaaaactg gtcacgtatt ccaacaatgg 901 aagtctgttc tagctgtcac gtagacatcg attttgctgc gggtaaaggc cactctcaac 961 aactcgataa ctccaactgt atcgcctgcc ataacagcga ctggactgct gagttacaca 1021 cagccaaaac caccgcaact aagaacttga ttaatcaata cggtatcgag actacctcga 1081 caattaatac cgaaactaaa gcagccacaa ttagtgttca agttgtagat gcgaacggta 1141 ctgctgttga tctcaagacc atcctgccta aagtgcaacg cttagagatc atcaccaacg 1201 ttggtcctaa taatgcaacc ttaggttata gtggcaaaga ttcaatattt gcaatcaaaa 1261 atggagctct tgatccaaaa gctactatca atgatgctgg caaactggtt tataccacta 1321 ctaaagacct caaacttggc caaaacggcg cagacagcga cacagcattt agctttgtag 1381 gttggtcaat gtgttctagc gaaggtaagt ttgtagactg tgcagaccct gcatttgatg 1441 gtgttgatgt aactaagtat accggcatga aagcggattt agcctttgct actttgtcag 1501 gtaaagcacc aagtactcgc cacgttgatt ctgttaacat gacagcctgt gccaattgcc 1561 acactgctga gttcgaaatt cacaaaggca aacaacatgc aggctttgtg atgacagagc 1621 aactatcaca cacccaagat gctaacggta aagcgattgt aggccttgac gcatgtgtga 1681 cttgtcatac tcctgatggc acctatagct ttgccaaccg tggtgcgcta gagctaaaac 1741 tacacaaaaa acacgttgaa gatgcctacg gcctcattgg tggcaattgt gcctcttgtc 1801 actcagactt caaccttgag tctttcaaga agaaaggcgc attgaatact gccgctgcaa 1861 cagataaaac aggtctatat tctacgccga tcactgcaac ttgtactacc tgtcacacag 1921 ttggcagcca gtacatggtc catacgaaag aaaccctgga gtctttcggt gcagttgttg 1981 atggcacaaa agatgatgct accagtgcgg cacagtcaga aacctgtttc tactgccata 2041 ccccaacagt tgcagatcac actaaagtga aaatgtaaat gaagaactgc ctaaaaatga 2101 aaaacctact gccggcactt accatcacaa tggcaatgtc tgcaattatg gcattagtcg 2161 tcacaccaaa cgcttatgcg tcgaagtggg atgagaaaat gacgccagag caagtcgaag 2221 ccaccttaga taagaagttt gccgaaggca actactcccc taaaggcgcc gattcttgct 2281 tgatgtgcca taagaaatcc gaaaaagtca tggacctttt caaaggtgtc cacggtgcga 2341 ttgactcctc taagagtcca atggctggcc tgcaatgtga ggcatgccac ggcccactgg 2401 gtcagcacaa caaaggcggc aacgagccga tgatcacttt tggtaagcaa tcaaccttaa 2461 gtgccgacaa gcaaaacagc gtatgtatga gctgtcacca agacgataag cgtatgtctt 2521 ggaatggcgg tcaccatgac aatgccgatg ttgcttgtgc ttcttgtcac caagtacacg 2581 tcgcaaaaga tcctgtgtta tctaaaaaca cggaaatgga agtctgtact agctgccata 2641 caaagcaaaa agcggatatg aataaacgct caagtcaccc actcaaatgg gcacaaatga 2701 cctgtagcga ctgtcacaat ccccatggga gcatgacaga ttccgatctt aacaagccta 2761 gcgtgaatga tacctgttat tcctgtcacg ccgaaaaacg cggcccaaaa ctttgggagc 2821 atgcacccgt cactgagaat tgtgtcactt gccacaatcc tcacggtagt gtgaatgacg 2881 gtatgctgaa aacccgtgcg ccacagctat gtcagcaatg tcacgccagc gatggccacg 2941 ccagcaacgc ctacttaggt aacactggat taggttcaaa tgtcggtgac aatgccttta 3001 ctggtggaag aagctgctta aattgccata gtcaggttca tggttctaac catccatctg 3061 gcaagctatt acagcgctaa atgaaattta aactcaattt gatcactcta gcgttattag 3121 ccaacacagg cttggccgtc gctgctgatg gttatggtct agcgaatgcc aatactgaaa 3181 aagtgaaatt atccgcatgg agctgtaaag gctgcgtcgt tgaaacgggc acatcaggca 3241 ctgtgggtgt cggtgtcggt tataacagcg aagaggatat tcgctctgcc aatgcctttg 3301 gtacatccaa tgaagtggcg ggtaaatttg atgccgattt aaactttaaa ggtgaaaagg 3361 gttatcgtgc cagtgttgat gcttatcaac tcggtatgga tggcggtcgc ttagatgtca 3421 atgcgggcaa acaaggccag tacaacgtca atgtgaacta tcgccaaatt gctacctacg 3481 acagcaatag cgccctatcg ccctacgcgg gtattggtgg caataacctc acgttaccgg 3541 ataactggat aacagcaggt tcaagcaacc aaatgccact cttgatggac agcctcaatg 3601 ccctcgaact ctcacttaaa cgtgagcgca cggggttggg atttgaatat caaggtgaat 3661 ccctgtggag cacctatgtt aactacatgc gtgaagagaa aaccggctta aaacaagcct 3721 ctggtagctt cttcaaccaa tcgatgatgt tagcagagcc ggtggattac accactgaca 3781 ccattgaagc gggtgtcaaa ctcaagggtg atcgttggtt taccgcactc agttacaatg 3841 ggtcaatatt caaaaacgaa tacaaccaat tggactttga aaatgctttt aaccccacct 3901 ttggtgctca aacccaaggt acgatggcac tcgatccgga taaccagtca cacaccgtgt 3961 cgctgatggg acagtacaac gatggcagca acgcactgtc gggtcgtatt ctgaccggac 4021 aaatgagcca agatcaggcg ttagtgacgg ataactaccg ttatgctaat cagctcaata 4081 ccgatgccgt cgatgccaaa gtcgatctac tgggtatgaa cctgaaagtc gttagcaaag 4141 tgagcaatga tcttcgctta acaggtagtt acgattatta cgaccgtgac aataataccc 4201 aagtagaaga atggactcag atcagcatca acaatgtcaa cggtaaggtg gcttataaca 4261 ccccttacga taatcgtacg caacgcttta aagttgccgc agattatcgc attacccgcg 4321 atatcaaact cgatggtggt tatgacttca aacgtgacca acgtgattat caagaccgtg 4381 aaaccacgga tgaaaatacc gtttgggccc gtttacgtgt aaacagcttc gatacttggg 4441 acatgtgggt aaaaggcagt tacggtaacc gtgacggctc acaataccaa gcgtctgaat 4501 ggacctcttc tgaaaccaac agcctgttac gtaagtacaa tctggctgac cgtgacagaa 4561 ctcaagtcga agcacggatc acccattcgc cattagaaag cctgactatc gatgttggtg 4621 cccgttacgc gttagatgat tataccgata ctgtgattgg attaactgag tcaaaagaca 4681 ccagttatga tgccaacatc agttatatga tcaccgctga cttactggca accgccttct 4741 acaattacca aaccattgag tctgaacagg cgggtagcag caattacagc accccaacgt 4801 ggacaggctt tatagaagat caggtagatg tggtcggtgc aggtatcagc tacaacaatc 4861 tgctggagaa caagttacgc ctaggactgg actacaccta ttccaactcc gacagtaaca 4921 ctcaagtcag acaaggtatc actggcgact atggtgatta ttttgccaaa gtgcataaca 4981 ttaacttata cgctcaatat caagccaccg agaaactcgc gctgcgcttc gattacaaaa 5041 ttgagaacta taaggacaat gacgccgcaa atgatatcgc cgttgatggc atttggaacg 5101 tcgtaggttt tggtagtaac agccatgact acaccgcaca aatgctgatg ctgagcatga 5161 gttacaaact ctaa //