LOCUS BBa_K1172405 5150 bp DNA linear 22-Nov-2024 DEFINITION mtrCAB with T7 Promoter and RBS ACCESSION BBa_K1172405 VERSION BBa_K1172405.1 FEATURES Location/Qualifiers misc_feature 21..32 /label=B0034 promoter 1..20 /label=T7 promoter RBS 24..27 /label=RBS promoter 1..32 /label=BBa_K525998 CDS 39..2054 /label=mtrC misc_feature 39..44 /label=start misc_feature 1836..1836 /label=PstI site G->A CDS 2055..3056 /label=mtrA misc_feature 2121..2121 /label=pstI site G->A CDS 3057..5150 /label=mtrB misc_feature 2055..2057 /label=start misc_feature 3057..3059 /label=start CDS 39..5150 /label=BBa_K1172401 ORIGIN 1 taatacgact cactataggg aaagaggaga aatactagat gatgaacgca caaaaatcaa 61 aaatcgcact gctgctcgca gcaagtgccg tcacaatggc cttaaccggc tgtggtggaa 121 gcgatggtaa taacggcaat gatggtagtg atggtggtga gccagcaggt agcatccaga 181 cgttaaacct agatatcact aaagtaagct atgaaaatgg tgcacctatg gtcactgttt 241 tcgccactaa cgaagccgac atgccagtga ttggtctcgc aaatttagaa atcaaaaaag 301 cactgcaatt aataccggaa ggggcgacag gcccaggtaa tagcgctaac tggcaaggct 361 taggctcatc aaagagctat gtcgataata aaaacggtag ctataccttt aaattcgacg 421 ccttcgatag taataaggtc tttaatgctc aattaacgca acgctttaac gttgtttctg 481 ctgcgggtaa attagcagac ggaacgaccg ttcccgttgc cgaaatggtt gaagatttcg 541 acggccaagg taatgcgccg caatatacaa aaaatatcgt tagccacgaa gtatgtgctt 601 cttgccacgt agaaggtgaa aagatttatc accaagctac tgaagtcgaa acttgtattt 661 cttgccacac tcaagagttt gcggatggtc gcggcaaacc ccatgtcgcc tttagtcact 721 taattcacaa tgtgcataat gccaacaaag cttggggcaa agacaataaa atccctacag 781 ttgcacaaaa tattgtccaa gataattgcc aagtttgtca cgttgaatcc gacatgctca 841 ccgaggcaaa aaactggtca cgtattccaa caatggaagt ctgttctagc tgtcacgtag 901 acatcgattt tgctgcgggt aaaggccact ctcaacaact cgataactcc aactgtatcg 961 cctgccataa cagcgactgg actgctgagt tacacacagc caaaaccacc gcaactaaga 1021 acttgattaa tcaatacggt atcgagacta cctcgacaat taataccgaa actaaagcag 1081 ccacaattag tgttcaagtt gtagatgcga acggtactgc tgttgatctc aagaccatcc 1141 tgcctaaagt gcaacgctta gagatcatca ccaacgttgg tcctaataat gcaaccttag 1201 gttatagtgg caaagattca atatttgcaa tcaaaaatgg agctcttgat ccaaaagcta 1261 ctatcaatga tgctggcaaa ctggtttata ccactactaa agacctcaaa cttggccaaa 1321 acggcgcaga cagcgacaca gcatttagct ttgtaggttg gtcaatgtgt tctagcgaag 1381 gtaagtttgt agactgtgca gaccctgcat ttgatggtgt tgatgtaact aagtataccg 1441 gcatgaaagc ggatttagcc tttgctactt tgtcaggtaa agcaccaagt actcgccacg 1501 ttgattctgt taacatgaca gcctgtgcca attgccacac tgctgagttc gaaattcaca 1561 aaggcaaaca acatgcaggc tttgtgatga cagagcaact atcacacacc caagatgcta 1621 acggtaaagc gattgtaggc cttgacgcat gtgtgacttg tcatactcct gatggcacct 1681 atagctttgc caaccgtggt gcgctagagc taaaactaca caaaaaacac gttgaagatg 1741 cctacggcct cattggtggc aattgtgcct cttgtcactc agacttcaac cttgagtctt 1801 tcaagaagaa aggcgcattg aatactgccg ctgcaacaga taaaacaggt ctatattcta 1861 cgccgatcac tgcaacttgt actacctgtc acacagttgg cagccagtac atggtccata 1921 cgaaagaaac cctggagtct ttcggtgcag ttgttgatgg cacaaaagat gatgctacca 1981 gtgcggcaca gtcagaaacc tgtttctact gccatacccc aacagttgca gatcacacta 2041 aagtgaaaat gtaaatgaag aactgcctaa aaatgaaaaa cctactgccg gcacttacca 2101 tcacaatggc aatgtctgca attatggcat tagtcgtcac accaaacgct tatgcgtcga 2161 agtgggatga gaaaatgacg ccagagcaag tcgaagccac cttagataag aagtttgccg 2221 aaggcaacta ctcccctaaa ggcgccgatt cttgcttgat gtgccataag aaatccgaaa 2281 aagtcatgga ccttttcaaa ggtgtccacg gtgcgattga ctcctctaag agtccaatgg 2341 ctggcctgca atgtgaggca tgccacggcc cactgggtca gcacaacaaa ggcggcaacg 2401 agccgatgat cacttttggt aagcaatcaa ccttaagtgc cgacaagcaa aacagcgtat 2461 gtatgagctg tcaccaagac gataagcgta tgtcttggaa tggcggtcac catgacaatg 2521 ccgatgttgc ttgtgcttct tgtcaccaag tacacgtcgc aaaagatcct gtgttatcta 2581 aaaacacgga aatggaagtc tgtactagct gccatacaaa gcaaaaagcg gatatgaata 2641 aacgctcaag tcacccactc aaatgggcac aaatgacctg tagcgactgt cacaatcccc 2701 atgggagcat gacagattcc gatcttaaca agcctagcgt gaatgatacc tgttattcct 2761 gtcacgccga aaaacgcggc ccaaaacttt gggagcatgc acccgtcact gagaattgtg 2821 tcacttgcca caatcctcac ggtagtgtga atgacggtat gctgaaaacc cgtgcgccac 2881 agctatgtca gcaatgtcac gccagcgatg gccacgccag caacgcctac ttaggtaaca 2941 ctggattagg ttcaaatgtc ggtgacaatg cctttactgg tggaagaagc tgcttaaatt 3001 gccatagtca ggttcatggt tctaaccatc catctggcaa gctattacag cgctaaatga 3061 aatttaaact caatttgatc actctagcgt tattagccaa cacaggcttg gccgtcgctg 3121 ctgatggtta tggtctagcg aatgccaata ctgaaaaagt gaaattatcc gcatggagct 3181 gtaaaggctg cgtcgttgaa acgggcacat caggcactgt gggtgtcggt gtcggttata 3241 acagcgaaga ggatattcgc tctgccaatg cctttggtac atccaatgaa gtggcgggta 3301 aatttgatgc cgatttaaac tttaaaggtg aaaagggtta tcgtgccagt gttgatgctt 3361 atcaactcgg tatggatggc ggtcgcttag atgtcaatgc gggcaaacaa ggccagtaca 3421 acgtcaatgt gaactatcgc caaattgcta cctacgacag caatagcgcc ctatcgccct 3481 acgcgggtat tggtggcaat aacctcacgt taccggataa ctggataaca gcaggttcaa 3541 gcaaccaaat gccactcttg atggacagcc tcaatgccct cgaactctca cttaaacgtg 3601 agcgcacggg gttgggattt gaatatcaag gtgaatccct gtggagcacc tatgttaact 3661 acatgcgtga agagaaaacc ggcttaaaac aagcctctgg tagcttcttc aaccaatcga 3721 tgatgttagc agagccggtg gattacacca ctgacaccat tgaagcgggt gtcaaactca 3781 agggtgatcg ttggtttacc gcactcagtt acaatgggtc aatattcaaa aacgaataca 3841 accaattgga ctttgaaaat gcttttaacc ccacctttgg tgctcaaacc caaggtacga 3901 tggcactcga tccggataac cagtcacaca ccgtgtcgct gatgggacag tacaacgatg 3961 gcagcaacgc actgtcgggt cgtattctga ccggacaaat gagccaagat caggcgttag 4021 tgacggataa ctaccgttat gctaatcagc tcaataccga tgccgtcgat gccaaagtcg 4081 atctactggg tatgaacctg aaagtcgtta gcaaagtgag caatgatctt cgcttaacag 4141 gtagttacga ttattacgac cgtgacaata atacccaagt agaagaatgg actcagatca 4201 gcatcaacaa tgtcaacggt aaggtggctt ataacacccc ttacgataat cgtacgcaac 4261 gctttaaagt tgccgcagat tatcgcatta cccgcgatat caaactcgat ggtggttatg 4321 acttcaaacg tgaccaacgt gattatcaag accgtgaaac cacggatgaa aataccgttt 4381 gggcccgttt acgtgtaaac agcttcgata cttgggacat gtgggtaaaa ggcagttacg 4441 gtaaccgtga cggctcacaa taccaagcgt ctgaatggac ctcttctgaa accaacagcc 4501 tgttacgtaa gtacaatctg gctgaccgtg acagaactca agtcgaagca cggatcaccc 4561 attcgccatt agaaagcctg actatcgatg ttggtgcccg ttacgcgtta gatgattata 4621 ccgatactgt gattggatta actgagtcaa aagacaccag ttatgatgcc aacatcagtt 4681 atatgatcac cgctgactta ctggcaaccg ccttctacaa ttaccaaacc attgagtctg 4741 aacaggcggg tagcagcaat tacagcaccc caacgtggac aggctttata gaagatcagg 4801 tagatgtggt cggtgcaggt atcagctaca acaatctgct ggagaacaag ttacgcctag 4861 gactggacta cacctattcc aactccgaca gtaacactca agtcagacaa ggtatcactg 4921 gcgactatgg tgattatttt gccaaagtgc ataacattaa cttatacgct caatatcaag 4981 ccaccgagaa actcgcgctg cgcttcgatt acaaaattga gaactataag gacaatgacg 5041 ccgcaaatga tatcgccgtt gatggcattt ggaacgtcgt aggttttggt agtaacagcc 5101 atgactacac cgcacaaatg ctgatgctga gcatgagtta caaactctaa //