BBa_K1172501 1 oprF Outer membrane porin OprF from Pseudomonas fluorescens 2013-08-27T11:00:00Z 2015-05-08T01:09:34Z Pseudomona fluorescens genomic DNA By heterologous expression of the pore-forming transmembrane-protein (or porine) OprF from Pseudomona fluorescens a further optimization of the E. coli membrane will be achieved. E. coli possesses several own porines, for example OmpF and OmpC. But these naturally occurring porines are only permeable for molecules smaller than 600 Da, which decreases the range of usable mediators significantly. Opposed to that, porine OprF from P. fluorescence forms one of the biggest known pores in the outer bacterial membrane with a permeability of up to 3000 Da, thus improving the electron shuttle mediated extracellular electron transfer (ETT) and enabling the usage of alternative mediators such as riboflavin (vitamine B2). false false _1485_ 0 16785 9 It's complicated true Primeroverlap for Gibson-Assembly false Lukas Rositzka annotation2370267 1 cds range2370267 1 1 1298 annotation2370296 1 stop range2370296 1 1296 1298 annotation2370295 1 start range2370295 1 1 3 BBa_K1172501_sequence 1 atgaaactgaaaaacaccttgggctttgcacaccggggaagtcggcaaacagattgttgaaactgcggttttcgccgtagatgaccaccacattcttcacgttatcgtgcagggccttgtccagctcagctggcgacagttcgcgggtgactaccggcccaggcggttcagcgggcccaccgcaggcgctcagggtcgccccggcgcccagtacggcaacccctcctaaaaagcgccgacggcttgaatcggggagcgtatcggcggcattttgggtggctttcgggtcttcggtttcttcggtcatccgaggcatctcacggctgtttttggtttatttgttgctggatgtttcgttgaaaaggcatcaaatatggcaaagcttaaacgcttcagtctataaggcgaggccttagggcataagggttctgatgatgactatcgggtttcgtaacgtggccgcgctgcaatgcaccctgtgccccgtgatcgattgacggtcgatcaaggggccagaggcggtgccgggcctgagggcgaagcttaagcaggcaatgtgacagaagcgtttacggcatcacgggaggcaggtatttgagcgtggtgcctagcgcccaaagcaggaacaacaccaacggcgtgtgcaccagcagttgcacaaacgagaaaccaatcaggtctcgtgccttcagccccaacacgccgagcaacggcagcatgtagaacgggttgatcaagttcggcagcgcctcggcagcgttgtagatctgtaccgcccagcccaagtggtattgcagctcattggccacttgcatcacgtaaggcgcttcgatgatccacttgccgccccccgatggaataaagaaacccagcaccgccgagtacacgcccatcaacaacgcataggtgtcgtgagaggcgatggaggtaaagaaggtcgagatatggtgcgccagggtctggccgtcgccacccttgaccacggtcatcagtgcagcgatcgagccatacagcggaaactggatcaacacgccggtggtggtcggcacggcacgcgccaccgcatcgaggaaactgcgcgggcgccagtgcagcagtgcgccaaccatgatgaacaggaagttataggtgttcagccctgaaatggcgctgatcgccggcttggtcgaaaactcatggaataaccaacctgccgccagcaatgcaagcagaatggtcagcagcgggctgtgctccaaccattcgcccggacggcttggcgcttgaatcttgcgcaggttgaagcccaagctaagtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z