BBa_K525998 1 pT7 Promoter T7 and RBS 2011-09-12T11:00:00Z 2015-05-08T01:12:35Z Synthesis Released HQ 2013 Promoter T7 and RBS false false _690_ 0 8543 9 In stock false Synthesis false Anna Drong annotation2127788 1 B0034 range2127788 1 21 32 annotation2129432 1 T7 promoter range2129432 1 1 20 annotation2129433 1 RBS range2129433 1 24 27 BBa_K1172502 1 BBa_K1172502 Outer membrane porin OprF from Pseudomonas fluorescens with PT7 and RBS 2013-08-27T11:00:00Z 2015-05-08T01:09:34Z false false _1485_ 0 16785 9 It's complicated false false Lukas Rositzka component2359415 1 BBa_K525998 component2359416 1 BBa_K1172501 annotation2359416 1 BBa_K1172501 range2359416 1 39 1336 annotation2359415 1 BBa_K525998 range2359415 1 1 32 BBa_K1172501 1 oprF Outer membrane porin OprF from Pseudomonas fluorescens 2013-08-27T11:00:00Z 2015-05-08T01:09:34Z Pseudomona fluorescens genomic DNA By heterologous expression of the pore-forming transmembrane-protein (or porine) OprF from Pseudomona fluorescens a further optimization of the E. coli membrane will be achieved. E. coli possesses several own porines, for example OmpF and OmpC. But these naturally occurring porines are only permeable for molecules smaller than 600 Da, which decreases the range of usable mediators significantly. Opposed to that, porine OprF from P. fluorescence forms one of the biggest known pores in the outer bacterial membrane with a permeability of up to 3000 Da, thus improving the electron shuttle mediated extracellular electron transfer (ETT) and enabling the usage of alternative mediators such as riboflavin (vitamine B2). false false _1485_ 0 16785 9 It's complicated true Primeroverlap for Gibson-Assembly false Lukas Rositzka annotation2370296 1 stop range2370296 1 1296 1298 annotation2370295 1 start range2370295 1 1 3 annotation2370267 1 cds range2370267 1 1 1298 BBa_K525998_sequence 1 taatacgactcactatagggaaagaggagaaa BBa_K1172501_sequence 1 atgaaactgaaaaacaccttgggctttgcacaccggggaagtcggcaaacagattgttgaaactgcggttttcgccgtagatgaccaccacattcttcacgttatcgtgcagggccttgtccagctcagctggcgacagttcgcgggtgactaccggcccaggcggttcagcgggcccaccgcaggcgctcagggtcgccccggcgcccagtacggcaacccctcctaaaaagcgccgacggcttgaatcggggagcgtatcggcggcattttgggtggctttcgggtcttcggtttcttcggtcatccgaggcatctcacggctgtttttggtttatttgttgctggatgtttcgttgaaaaggcatcaaatatggcaaagcttaaacgcttcagtctataaggcgaggccttagggcataagggttctgatgatgactatcgggtttcgtaacgtggccgcgctgcaatgcaccctgtgccccgtgatcgattgacggtcgatcaaggggccagaggcggtgccgggcctgagggcgaagcttaagcaggcaatgtgacagaagcgtttacggcatcacgggaggcaggtatttgagcgtggtgcctagcgcccaaagcaggaacaacaccaacggcgtgtgcaccagcagttgcacaaacgagaaaccaatcaggtctcgtgccttcagccccaacacgccgagcaacggcagcatgtagaacgggttgatcaagttcggcagcgcctcggcagcgttgtagatctgtaccgcccagcccaagtggtattgcagctcattggccacttgcatcacgtaaggcgcttcgatgatccacttgccgccccccgatggaataaagaaacccagcaccgccgagtacacgcccatcaacaacgcataggtgtcgtgagaggcgatggaggtaaagaaggtcgagatatggtgcgccagggtctggccgtcgccacccttgaccacggtcatcagtgcagcgatcgagccatacagcggaaactggatcaacacgccggtggtggtcggcacggcacgcgccaccgcatcgaggaaactgcgcgggcgccagtgcagcagtgcgccaaccatgatgaacaggaagttataggtgttcagccctgaaatggcgctgatcgccggcttggtcgaaaactcatggaataaccaacctgccgccagcaatgcaagcagaatggtcagcagcgggctgtgctccaaccattcgcccggacggcttggcgcttgaatcttgcgcaggttgaagcccaagctaagtaa BBa_K1172502_sequence 1 taatacgactcactatagggaaagaggagaaatactagatgaaactgaaaaacaccttgggctttgcacaccggggaagtcggcaaacagattgttgaaactgcggttttcgccgtagatgaccaccacattcttcacgttatcgtgcagggccttgtccagctcagctggcgacagttcgcgggtgactaccggcccaggcggttcagcgggcccaccgcaggcgctcagggtcgccccggcgcccagtacggcaacccctcctaaaaagcgccgacggcttgaatcggggagcgtatcggcggcattttgggtggctttcgggtcttcggtttcttcggtcatccgaggcatctcacggctgtttttggtttatttgttgctggatgtttcgttgaaaaggcatcaaatatggcaaagcttaaacgcttcagtctataaggcgaggccttagggcataagggttctgatgatgactatcgggtttcgtaacgtggccgcgctgcaatgcaccctgtgccccgtgatcgattgacggtcgatcaaggggccagaggcggtgccgggcctgagggcgaagcttaagcaggcaatgtgacagaagcgtttacggcatcacgggaggcaggtatttgagcgtggtgcctagcgcccaaagcaggaacaacaccaacggcgtgtgcaccagcagttgcacaaacgagaaaccaatcaggtctcgtgccttcagccccaacacgccgagcaacggcagcatgtagaacgggttgatcaagttcggcagcgcctcggcagcgttgtagatctgtaccgcccagcccaagtggtattgcagctcattggccacttgcatcacgtaaggcgcttcgatgatccacttgccgccccccgatggaataaagaaacccagcaccgccgagtacacgcccatcaacaacgcataggtgtcgtgagaggcgatggaggtaaagaaggtcgagatatggtgcgccagggtctggccgtcgccacccttgaccacggtcatcagtgcagcgatcgagccatacagcggaaactggatcaacacgccggtggtggtcggcacggcacgcgccaccgcatcgaggaaactgcgcgggcgccagtgcagcagtgcgccaaccatgatgaacaggaagttataggtgttcagccctgaaatggcgctgatcgccggcttggtcgaaaactcatggaataaccaacctgccgccagcaatgcaagcagaatggtcagcagcgggctgtgctccaaccattcgcccggacggcttggcgcttgaatcttgcgcaggttgaagcccaagctaagtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z