LOCUS       BBa_K1172503            1524 bp    DNA     linear       17-May-2024
DEFINITION  Outer membrane porin OprF from Pseudomonas fluorescens with Plac 
            and RBS
ACCESSION   BBa_K1172503
VERSION     BBa_K1172503.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..1524
                     /label=cds
     misc_feature    227..229
                     /label=start
     misc_feature    1522..1524
                     /label=stop
     CDS             227..1524
                     /label=BBa_K1172501
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aactgaaaaa
      241 caccttgggc tttgcacacc ggggaagtcg gcaaacagat tgttgaaact gcggttttcg
      301 ccgtagatga ccaccacatt cttcacgtta tcgtgcaggg ccttgtccag ctcagctggc
      361 gacagttcgc gggtgactac cggcccaggc ggttcagcgg gcccaccgca ggcgctcagg
      421 gtcgccccgg cgcccagtac ggcaacccct cctaaaaagc gccgacggct tgaatcgggg
      481 agcgtatcgg cggcattttg ggtggctttc gggtcttcgg tttcttcggt catccgaggc
      541 atctcacggc tgtttttggt ttatttgttg ctggatgttt cgttgaaaag gcatcaaata
      601 tggcaaagct taaacgcttc agtctataag gcgaggcctt agggcataag ggttctgatg
      661 atgactatcg ggtttcgtaa cgtggccgcg ctgcaatgca ccctgtgccc cgtgatcgat
      721 tgacggtcga tcaaggggcc agaggcggtg ccgggcctga gggcgaagct taagcaggca
      781 atgtgacaga agcgtttacg gcatcacggg aggcaggtat ttgagcgtgg tgcctagcgc
      841 ccaaagcagg aacaacacca acggcgtgtg caccagcagt tgcacaaacg agaaaccaat
      901 caggtctcgt gccttcagcc ccaacacgcc gagcaacggc agcatgtaga acgggttgat
      961 caagttcggc agcgcctcgg cagcgttgta gatctgtacc gcccagccca agtggtattg
     1021 cagctcattg gccacttgca tcacgtaagg cgcttcgatg atccacttgc cgccccccga
     1081 tggaataaag aaacccagca ccgccgagta cacgcccatc aacaacgcat aggtgtcgtg
     1141 agaggcgatg gaggtaaaga aggtcgagat atggtgcgcc agggtctggc cgtcgccacc
     1201 cttgaccacg gtcatcagtg cagcgatcga gccatacagc ggaaactgga tcaacacgcc
     1261 ggtggtggtc ggcacggcac gcgccaccgc atcgaggaaa ctgcgcgggc gccagtgcag
     1321 cagtgcgcca accatgatga acaggaagtt ataggtgttc agccctgaaa tggcgctgat
     1381 cgccggcttg gtcgaaaact catggaataa ccaacctgcc gccagcaatg caagcagaat
     1441 ggtcagcagc gggctgtgct ccaaccattc gcccggacgg cttggcgctt gaatcttgcg
     1501 caggttgaag cccaagctaa gtaa
//