BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J23104 1 BBa_J23104 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z isolated from library of promoters replace later false false _52_ 0 483 95 In stock true N/A true John Anderson BBa_K1172507 1 BBa_K1172507 Outer membrane porin OprF from Pseudomonas fluorescens with strong Anderson promoter and strong RBS 2013-08-27T11:00:00Z 2015-05-08T01:09:34Z false false _1485_ 0 16785 9 It's complicated false false Lukas Rositzka component2361220 1 BBa_K608002 component2361221 1 BBa_K1172501 annotation2361221 1 BBa_K1172501 range2361221 1 62 1359 annotation2361220 1 BBa_K608002 range2361220 1 1 55 BBa_K608002 1 BBa_K608002 strong Promoter and strong RBS 2011-09-14T11:00:00Z 2015-05-08T01:12:52Z assembly from iGEM parts Released HQ 2013 you can insert your part behind this part so it will be immediatly expressed in e.coli. false false _780_ 0 9115 9 In stock false cloning via 3a-assembly false Julia M??ller component2128646 1 BBa_J23104 component2128648 1 BBa_B0034 annotation2128648 1 BBa_B0034 range2128648 1 44 55 annotation2128646 1 BBa_J23104 range2128646 1 1 35 BBa_K1172501 1 oprF Outer membrane porin OprF from Pseudomonas fluorescens 2013-08-27T11:00:00Z 2015-05-08T01:09:34Z Pseudomona fluorescens genomic DNA By heterologous expression of the pore-forming transmembrane-protein (or porine) OprF from Pseudomona fluorescens a further optimization of the E. coli membrane will be achieved. E. coli possesses several own porines, for example OmpF and OmpC. But these naturally occurring porines are only permeable for molecules smaller than 600 Da, which decreases the range of usable mediators significantly. Opposed to that, porine OprF from P. fluorescence forms one of the biggest known pores in the outer bacterial membrane with a permeability of up to 3000 Da, thus improving the electron shuttle mediated extracellular electron transfer (ETT) and enabling the usage of alternative mediators such as riboflavin (vitamine B2). false false _1485_ 0 16785 9 It's complicated true Primeroverlap for Gibson-Assembly false Lukas Rositzka annotation2370267 1 cds range2370267 1 1 1298 annotation2370296 1 stop range2370296 1 1296 1298 annotation2370295 1 start range2370295 1 1 3 BBa_B0034_sequence 1 aaagaggagaaa BBa_K608002_sequence 1 ttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaa BBa_K1172507_sequence 1 ttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaatactagatgaaactgaaaaacaccttgggctttgcacaccggggaagtcggcaaacagattgttgaaactgcggttttcgccgtagatgaccaccacattcttcacgttatcgtgcagggccttgtccagctcagctggcgacagttcgcgggtgactaccggcccaggcggttcagcgggcccaccgcaggcgctcagggtcgccccggcgcccagtacggcaacccctcctaaaaagcgccgacggcttgaatcggggagcgtatcggcggcattttgggtggctttcgggtcttcggtttcttcggtcatccgaggcatctcacggctgtttttggtttatttgttgctggatgtttcgttgaaaaggcatcaaatatggcaaagcttaaacgcttcagtctataaggcgaggccttagggcataagggttctgatgatgactatcgggtttcgtaacgtggccgcgctgcaatgcaccctgtgccccgtgatcgattgacggtcgatcaaggggccagaggcggtgccgggcctgagggcgaagcttaagcaggcaatgtgacagaagcgtttacggcatcacgggaggcaggtatttgagcgtggtgcctagcgcccaaagcaggaacaacaccaacggcgtgtgcaccagcagttgcacaaacgagaaaccaatcaggtctcgtgccttcagccccaacacgccgagcaacggcagcatgtagaacgggttgatcaagttcggcagcgcctcggcagcgttgtagatctgtaccgcccagcccaagtggtattgcagctcattggccacttgcatcacgtaaggcgcttcgatgatccacttgccgccccccgatggaataaagaaacccagcaccgccgagtacacgcccatcaacaacgcataggtgtcgtgagaggcgatggaggtaaagaaggtcgagatatggtgcgccagggtctggccgtcgccacccttgaccacggtcatcagtgcagcgatcgagccatacagcggaaactggatcaacacgccggtggtggtcggcacggcacgcgccaccgcatcgaggaaactgcgcgggcgccagtgcagcagtgcgccaaccatgatgaacaggaagttataggtgttcagccctgaaatggcgctgatcgccggcttggtcgaaaactcatggaataaccaacctgccgccagcaatgcaagcagaatggtcagcagcgggctgtgctccaaccattcgcccggacggcttggcgcttgaatcttgcgcaggttgaagcccaagctaagtaa BBa_K1172501_sequence 1 atgaaactgaaaaacaccttgggctttgcacaccggggaagtcggcaaacagattgttgaaactgcggttttcgccgtagatgaccaccacattcttcacgttatcgtgcagggccttgtccagctcagctggcgacagttcgcgggtgactaccggcccaggcggttcagcgggcccaccgcaggcgctcagggtcgccccggcgcccagtacggcaacccctcctaaaaagcgccgacggcttgaatcggggagcgtatcggcggcattttgggtggctttcgggtcttcggtttcttcggtcatccgaggcatctcacggctgtttttggtttatttgttgctggatgtttcgttgaaaaggcatcaaatatggcaaagcttaaacgcttcagtctataaggcgaggccttagggcataagggttctgatgatgactatcgggtttcgtaacgtggccgcgctgcaatgcaccctgtgccccgtgatcgattgacggtcgatcaaggggccagaggcggtgccgggcctgagggcgaagcttaagcaggcaatgtgacagaagcgtttacggcatcacgggaggcaggtatttgagcgtggtgcctagcgcccaaagcaggaacaacaccaacggcgtgtgcaccagcagttgcacaaacgagaaaccaatcaggtctcgtgccttcagccccaacacgccgagcaacggcagcatgtagaacgggttgatcaagttcggcagcgcctcggcagcgttgtagatctgtaccgcccagcccaagtggtattgcagctcattggccacttgcatcacgtaaggcgcttcgatgatccacttgccgccccccgatggaataaagaaacccagcaccgccgagtacacgcccatcaacaacgcataggtgtcgtgagaggcgatggaggtaaagaaggtcgagatatggtgcgccagggtctggccgtcgccacccttgaccacggtcatcagtgcagcgatcgagccatacagcggaaactggatcaacacgccggtggtggtcggcacggcacgcgccaccgcatcgaggaaactgcgcgggcgccagtgcagcagtgcgccaaccatgatgaacaggaagttataggtgttcagccctgaaatggcgctgatcgccggcttggtcgaaaactcatggaataaccaacctgccgccagcaatgcaagcagaatggtcagcagcgggctgtgctccaaccattcgcccggacggcttggcgcttgaatcttgcgcaggttgaagcccaagctaagtaa BBa_J23104_sequence 1 ttgacagctagctcagtcctaggtattgtgctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z