LOCUS BBa_K1172588 4939 bp DNA linear 26-Nov-2024 DEFINITION Combination of oprF and rib-synth coding sequence under control of T7 promoter ACCESSION BBa_K1172588 VERSION BBa_K1172588.1 FEATURES Location/Qualifiers misc_feature 21..32 /label=B0034 promoter 1..20 /label=T7 promoter RBS 24..27 /label=RBS promoter 1..32 /label=BBa_K525998 CDS 39..1336 /label=cds misc_feature 39..41 /label=start misc_feature 1334..1336 /label=stop CDS 39..1336 /label=BBa_K1172501 misc_feature 1..1336 /label=BBa_K1172502 CDS 1343..2488 /label=ribD CDS 2496..3152 /label=SO_3468 CDS 3219..4322 /label=ribBA CDS 4463..4939 /label=ribE misc_feature 1576..1581 /label=Pst1 C->T misc_feature 2986..2991 /label=Pst1 C->T misc_feature 3182..3187 /label=EcoR1 C->T misc_feature 1343..1345 /label=atg misc_feature 2496..2498 /label=atg misc_feature 3219..3221 /label=atg misc_feature 4463..4465 /label=atg misc_feature 2486..2488 /label=tga misc_feature 3150..3152 /label=tag misc_feature 4320..4322 /label=taa misc_feature 4937..4939 /label=taa CDS 1343..4939 /label=BBa_K1172303 ORIGIN 1 taatacgact cactataggg aaagaggaga aatactagat gaaactgaaa aacaccttgg 61 gctttgcaca ccggggaagt cggcaaacag attgttgaaa ctgcggtttt cgccgtagat 121 gaccaccaca ttcttcacgt tatcgtgcag ggccttgtcc agctcagctg gcgacagttc 181 gcgggtgact accggcccag gcggttcagc gggcccaccg caggcgctca gggtcgcccc 241 ggcgcccagt acggcaaccc ctcctaaaaa gcgccgacgg cttgaatcgg ggagcgtatc 301 ggcggcattt tgggtggctt tcgggtcttc ggtttcttcg gtcatccgag gcatctcacg 361 gctgtttttg gtttatttgt tgctggatgt ttcgttgaaa aggcatcaaa tatggcaaag 421 cttaaacgct tcagtctata aggcgaggcc ttagggcata agggttctga tgatgactat 481 cgggtttcgt aacgtggccg cgctgcaatg caccctgtgc cccgtgatcg attgacggtc 541 gatcaagggg ccagaggcgg tgccgggcct gagggcgaag cttaagcagg caatgtgaca 601 gaagcgttta cggcatcacg ggaggcaggt atttgagcgt ggtgcctagc gcccaaagca 661 ggaacaacac caacggcgtg tgcaccagca gttgcacaaa cgagaaacca atcaggtctc 721 gtgccttcag ccccaacacg ccgagcaacg gcagcatgta gaacgggttg atcaagttcg 781 gcagcgcctc ggcagcgttg tagatctgta ccgcccagcc caagtggtat tgcagctcat 841 tggccacttg catcacgtaa ggcgcttcga tgatccactt gccgcccccc gatggaataa 901 agaaacccag caccgccgag tacacgccca tcaacaacgc ataggtgtcg tgagaggcga 961 tggaggtaaa gaaggtcgag atatggtgcg ccagggtctg gccgtcgcca cccttgacca 1021 cggtcatcag tgcagcgatc gagccataca gcggaaactg gatcaacacg ccggtggtgg 1081 tcggcacggc acgcgccacc gcatcgagga aactgcgcgg gcgccagtgc agcagtgcgc 1141 caaccatgat gaacaggaag ttataggtgt tcagccctga aatggcgctg atcgccggct 1201 tggtcgaaaa ctcatggaat aaccaacctg ccgccagcaa tgcaagcaga atggtcagca 1261 gcgggctgtg ctccaaccat tcgcccggac ggcttggcgc ttgaatcttg cgcaggttga 1321 agcccaagct aagtaatact agatgaattg gtcagtactc gataaccaaa tgatgagccg 1381 agctattcaa ttggctcgca aaggatttta taccactcgc cccaatccca gtgtgggctg 1441 cgttatcgtt aacgataacc agattgtcgg tgaaggttat catcaaaaag ccggagagcc 1501 ccacgctgaa gtgcatgcgc tgcgcatggc tggcgagctt gcccgtggcg cgactgctta 1561 tgtcacctta gaaccctgta gccattatgg tcgtacgccg ccctgcgcct tggcgctgat 1621 caatattggt gtaaagcggg tggtggtggc ggttgaagat cctaatccgc aggtcggcgg 1681 gcgcggtatt caaatgctgc gcgatgcggg tattcaagtg gatgtgggct tgcatcgtga 1741 tgaggcctat gccttaaatc ttggttttat gaagcgcatg gagtcgggtc taccttgggt 1801 gacggtaaag cttgccgcga gtcttgatgg taaaactgcg ttatcaaacg gggtgtctaa 1861 atggatcact ggccctgaag ctcgccgcga tgtgcagcgc ttaagattac gatcctgcgc 1921 gctgatcacg gggattgaaa cggtactggc cgatgatcca tcgctcaatg tgcgctactc 1981 agagcttggc agtttaagag cgcaattgag tgagtcgcag gttttacagc cgctgcgagt 2041 ggttttggac agtcgcgccc gcatgcctat cacggcagca ttgtttgcga ttgaatcacc 2101 cattttatta gtctcgacag tgagctattc gtcagacttt atggtgcaat tacccgccca 2161 tgtgacttgc ctccaattac cagccattga gggtcgaatc tccttgtctg cactattaag 2221 ctatttaggc aaaagctgta atcaggtgct tatcgaagcg ggcgcgactt tagctggcgc 2281 ctttatcggc tcaggattag ccgatgaatt agtgctttat caagcgatga aaatccttgg 2341 cggccaagga cgtaatctac ttgaattacc cgattatcaa atgatggccg atattccgac 2401 cctcaaattg gtcgacgagc gtaaagtggg cgcggatacg cgcttcacct tgaggctcga 2461 gtccaatcca tctttagcga ataagtgagt taaccatgtt tactgggatt attgaggccg 2521 ttggtacact gcgaaagctt gagcgtaaag gcgatgatat tcgcttaacg gtcgccagtg 2581 gcaaactgga tttaaccgat gtgcgtttag gcgacagtat cgccaccaac ggtgtatgtt 2641 tgaccgtggt tcaacagtta gccgatggct atgtggctga tgtgtcagct gaaaccgtca 2701 gccttacagg ttttgcaaac tataaagtgg gtactaaggt caatcttgaa aaggccgtca 2761 ccccaactac tcgtttaggc ggccatatgg tgagtggtca tgtggatggg attgctactg 2821 ttgagcagcg tcttgctcgt ggtcaagcaa tcgaattttg gttagcggct cccacggagc 2881 ttgctcgcta tattgcccat aaaggttcta tcacgattga tggcgtgagc ctgacagtaa 2941 acgaagtcga tggacaccgt tttcgattaa cgattgtgcc ccatactgta ggtgaaacca 3001 cgttggtgga tttaaaagca ggcgataagg tcaatattga agtggactta attgcccgtt 3061 atttagagcg tttaatgcgt tttgacagca aagaaaccca aggcggaggg gtgaccatgg 3121 aaatgttagc ccgtgctggc tttgtgcgtt agtacactgg cactcagttc acttaggtat 3181 agaatttata acaacagtca cattataaag gtcctacaat ggcgctgcac agtatagaag 3241 agatcatcga ggatattcgt caaggcaaaa tggttatttt gatggatgac gaggacagag 3301 aaaacgaagg cgatctgatt ttggcggccg aaatggtgac gccagaagcg attaacttta 3361 tggcgaaata cggccgcggg ttaatctgcc aaacgatgac caaggcccgt tgccagcagt 3421 taaatctgcc cttgatggta accaataaca atgcgcagtt ctcgaccaac tttacggtat 3481 cgattgaagc tgccgaaggc gtaaccacag ggatttcggc ccacgatcgc gctgtaaccg 3541 ttaaagcggc cgtagcaaaa gatgctaaag cgtcggactt agttcagcct gggcatatct 3601 tcccgttaat ggcgcaggat ggtggcgtat taacccgcgc aggtcacact gaagcgggct 3661 gtgatttagc ccgtttagca gggcttgaac catcgggggt gattgttgaa attttgaacg 3721 aagacggcac tatggcccgt cgcccagatt tagagatttt ctctgaaaaa catggcatta 3781 aaatcggcac tattgcggcc ctgattgaat accgtaatac caaagaaacg accgttgtgc 3841 gtgaagccaa atgcaaactg ccaacccgtt tcggtgagtt cgatatggtg acctttaggg 3901 atactatcga caatcaattg cactttgcct tagtgaaagg cgaggtgaag cctgactgtt 3961 tagtacgggt acatttacag aataccttta acgatctact tcactctgag cgtgatcagc 4021 aacgtagctg gccgctcgaa aaagcgatgg cgcggatttc tgccgaaggt ggtgtattag 4081 tactgcttgg caatcaggaa cattccagtg aaatcctcgc caaagtcaaa gcctttgaag 4141 cggaagatca aggtcagacc ccagtatcgg ctaaatggca aggcacctca cgccgtgtag 4201 gtgtgggttc gcaaatcctc gccagtttag gcgtgacaaa aatgcgactc ttaagctcgc 4261 ccaaacgtta tcactcactt tcaggctttg gccttgaagt gaccgaatgc gtagctgagt 4321 aacaggttaa gcttgtaata ggttgcacta aggtaaaatt aaccatttct atcatttgca 4381 taaggccgcg ggcaattgac tgtggtatca tgtcgccact tttcgcccag agctgggtgc 4441 tttagctaaa ttaggtaaga aaatgaacgt agttcaaggt aatatcgaag cgaagaatgc 4501 caaagttgcg attgtaattt cgcgttttaa cagcttttta gtcgaaagcc tgcttgaagg 4561 tgcacttgac acgctgaaac gttttggcca agtcagtgat gaaaacatca ctgttgtccg 4621 tgtacctggt gcggttgagt taccgctggc tgcacgtcgt gttgccgcaa gtggtaagtt 4681 tgacggtatt attgcacttg gtgcggtaat ccgtggtggt acccctcact ttgattttgt 4741 tgcaggcgaa tgtaacaaag gtctggctca aattgcatta gagttcgatc tgcccgttgc 4801 tttcggtgta ttgactacag ataccattga acaagctatt gagcgttcag gtactaaagc 4861 gggtaacaaa ggcggcgaag ctgctctaag cttgcttgaa atggtcaacg ttctgcaaca 4921 gcttgaacaa cagttgtaa //