BBa_K1172588 1 BBa_K1172588 Combination of oprF and rib-synth coding sequence under control of T7 promoter 2013-10-24T11:00:00Z 2015-05-08T01:09:35Z false false _1485_ 0 17241 9 It's complicated false false Thorben Meyer component2370611 1 BBa_K1172303 component2370606 1 BBa_K1172502 annotation2370606 1 BBa_K1172502 range2370606 1 1 1336 annotation2370611 1 BBa_K1172303 range2370611 1 1343 4939 BBa_K1172502 1 BBa_K1172502 Outer membrane porin OprF from Pseudomonas fluorescens with PT7 and RBS 2013-08-27T11:00:00Z 2015-05-08T01:09:34Z false false _1485_ 0 16785 9 It's complicated false false Lukas Rositzka component2359415 1 BBa_K525998 component2359416 1 BBa_K1172501 annotation2359415 1 BBa_K525998 range2359415 1 1 32 annotation2359416 1 BBa_K1172501 range2359416 1 39 1336 BBa_K1172303 1 rib-synth Riboflavin synthesis gene cluster from shewanella oneidensis 2013-09-18T11:00:00Z 2015-05-08T01:09:34Z genome of shewanella oneidensis MR-1 This gene cluster consists of four different genes that form a single operon. false false _1485_ 0 17241 9 In stock true we eliminated three illegal restriction sites. false Thorben Meyer annotation2370721 1 tga range2370721 1 1144 1146 annotation2370723 1 taa range2370723 1 2978 2980 annotation2370313 1 ribE range2370313 1 3121 3597 annotation2370724 1 taa range2370724 1 3595 3597 annotation2370718 1 atg range2370718 1 1154 1156 annotation2370310 1 ribD range2370310 1 1 1146 annotation2370719 1 atg range2370719 1 1877 1879 annotation2370714 1 Pst1 C->T range2370714 1 234 239 annotation2370312 1 ribBA range2370312 1 1877 2980 annotation2370311 1 SO_3468 range2370311 1 1154 1810 annotation2370716 1 EcoR1 C->T range2370716 1 1840 1845 annotation2370715 1 Pst1 C->T range2370715 1 1644 1649 annotation2370717 1 atg range2370717 1 1 3 annotation2370720 1 atg range2370720 1 3121 3123 annotation2370722 1 tag range2370722 1 1808 1810 BBa_K525998 1 pT7 Promoter T7 and RBS 2011-09-12T11:00:00Z 2015-05-08T01:12:35Z Synthesis Released HQ 2013 Promoter T7 and RBS false false _690_ 0 8543 9 In stock false Synthesis false Anna Drong annotation2129432 1 T7 promoter range2129432 1 1 20 annotation2127788 1 B0034 range2127788 1 21 32 annotation2129433 1 RBS range2129433 1 24 27 BBa_K1172501 1 oprF Outer membrane porin OprF from Pseudomonas fluorescens 2013-08-27T11:00:00Z 2015-05-08T01:09:34Z Pseudomona fluorescens genomic DNA By heterologous expression of the pore-forming transmembrane-protein (or porine) OprF from Pseudomona fluorescens a further optimization of the E. coli membrane will be achieved. E. coli possesses several own porines, for example OmpF and OmpC. But these naturally occurring porines are only permeable for molecules smaller than 600 Da, which decreases the range of usable mediators significantly. Opposed to that, porine OprF from P. fluorescence forms one of the biggest known pores in the outer bacterial membrane with a permeability of up to 3000 Da, thus improving the electron shuttle mediated extracellular electron transfer (ETT) and enabling the usage of alternative mediators such as riboflavin (vitamine B2). false false _1485_ 0 16785 9 It's complicated true Primeroverlap for Gibson-Assembly false Lukas Rositzka annotation2370267 1 cds range2370267 1 1 1298 annotation2370295 1 start range2370295 1 1 3 annotation2370296 1 stop range2370296 1 1296 1298 BBa_K1172303_sequence 1 atgaattggtcagtactcgataaccaaatgatgagccgagctattcaattggctcgcaaaggattttataccactcgccccaatcccagtgtgggctgcgttatcgttaacgataaccagattgtcggtgaaggttatcatcaaaaagccggagagccccacgctgaagtgcatgcgctgcgcatggctggcgagcttgcccgtggcgcgactgcttatgtcaccttagaaccctgtagccattatggtcgtacgccgccctgcgccttggcgctgatcaatattggtgtaaagcgggtggtggtggcggttgaagatcctaatccgcaggtcggcgggcgcggtattcaaatgctgcgcgatgcgggtattcaagtggatgtgggcttgcatcgtgatgaggcctatgccttaaatcttggttttatgaagcgcatggagtcgggtctaccttgggtgacggtaaagcttgccgcgagtcttgatggtaaaactgcgttatcaaacggggtgtctaaatggatcactggccctgaagctcgccgcgatgtgcagcgcttaagattacgatcctgcgcgctgatcacggggattgaaacggtactggccgatgatccatcgctcaatgtgcgctactcagagcttggcagtttaagagcgcaattgagtgagtcgcaggttttacagccgctgcgagtggttttggacagtcgcgcccgcatgcctatcacggcagcattgtttgcgattgaatcacccattttattagtctcgacagtgagctattcgtcagactttatggtgcaattacccgcccatgtgacttgcctccaattaccagccattgagggtcgaatctccttgtctgcactattaagctatttaggcaaaagctgtaatcaggtgcttatcgaagcgggcgcgactttagctggcgcctttatcggctcaggattagccgatgaattagtgctttatcaagcgatgaaaatccttggcggccaaggacgtaatctacttgaattacccgattatcaaatgatggccgatattccgaccctcaaattggtcgacgagcgtaaagtgggcgcggatacgcgcttcaccttgaggctcgagtccaatccatctttagcgaataagtgagttaaccatgtttactgggattattgaggccgttggtacactgcgaaagcttgagcgtaaaggcgatgatattcgcttaacggtcgccagtggcaaactggatttaaccgatgtgcgtttaggcgacagtatcgccaccaacggtgtatgtttgaccgtggttcaacagttagccgatggctatgtggctgatgtgtcagctgaaaccgtcagccttacaggttttgcaaactataaagtgggtactaaggtcaatcttgaaaaggccgtcaccccaactactcgtttaggcggccatatggtgagtggtcatgtggatgggattgctactgttgagcagcgtcttgctcgtggtcaagcaatcgaattttggttagcggctcccacggagcttgctcgctatattgcccataaaggttctatcacgattgatggcgtgagcctgacagtaaacgaagtcgatggacaccgttttcgattaacgattgtgccccatactgtaggtgaaaccacgttggtggatttaaaagcaggcgataaggtcaatattgaagtggacttaattgcccgttatttagagcgtttaatgcgttttgacagcaaagaaacccaaggcggaggggtgaccatggaaatgttagcccgtgctggctttgtgcgttagtacactggcactcagttcacttaggtatagaatttataacaacagtcacattataaaggtcctacaatggcgctgcacagtatagaagagatcatcgaggatattcgtcaaggcaaaatggttattttgatggatgacgaggacagagaaaacgaaggcgatctgattttggcggccgaaatggtgacgccagaagcgattaactttatggcgaaatacggccgcgggttaatctgccaaacgatgaccaaggcccgttgccagcagttaaatctgcccttgatggtaaccaataacaatgcgcagttctcgaccaactttacggtatcgattgaagctgccgaaggcgtaaccacagggatttcggcccacgatcgcgctgtaaccgttaaagcggccgtagcaaaagatgctaaagcgtcggacttagttcagcctgggcatatcttcccgttaatggcgcaggatggtggcgtattaacccgcgcaggtcacactgaagcgggctgtgatttagcccgtttagcagggcttgaaccatcgggggtgattgttgaaattttgaacgaagacggcactatggcccgtcgcccagatttagagattttctctgaaaaacatggcattaaaatcggcactattgcggccctgattgaataccgtaataccaaagaaacgaccgttgtgcgtgaagccaaatgcaaactgccaacccgtttcggtgagttcgatatggtgacctttagggatactatcgacaatcaattgcactttgccttagtgaaaggcgaggtgaagcctgactgtttagtacgggtacatttacagaatacctttaacgatctacttcactctgagcgtgatcagcaacgtagctggccgctcgaaaaagcgatggcgcggatttctgccgaaggtggtgtattagtactgcttggcaatcaggaacattccagtgaaatcctcgccaaagtcaaagcctttgaagcggaagatcaaggtcagaccccagtatcggctaaatggcaaggcacctcacgccgtgtaggtgtgggttcgcaaatcctcgccagtttaggcgtgacaaaaatgcgactcttaagctcgcccaaacgttatcactcactttcaggctttggccttgaagtgaccgaatgcgtagctgagtaacaggttaagcttgtaataggttgcactaaggtaaaattaaccatttctatcatttgcataaggccgcgggcaattgactgtggtatcatgtcgccacttttcgcccagagctgggtgctttagctaaattaggtaagaaaatgaacgtagttcaaggtaatatcgaagcgaagaatgccaaagttgcgattgtaatttcgcgttttaacagctttttagtcgaaagcctgcttgaaggtgcacttgacacgctgaaacgttttggccaagtcagtgatgaaaacatcactgttgtccgtgtacctggtgcggttgagttaccgctggctgcacgtcgtgttgccgcaagtggtaagtttgacggtattattgcacttggtgcggtaatccgtggtggtacccctcactttgattttgttgcaggcgaatgtaacaaaggtctggctcaaattgcattagagttcgatctgcccgttgctttcggtgtattgactacagataccattgaacaagctattgagcgttcaggtactaaagcgggtaacaaaggcggcgaagctgctctaagcttgcttgaaatggtcaacgttctgcaacagcttgaacaacagttgtaa BBa_K525998_sequence 1 taatacgactcactatagggaaagaggagaaa BBa_K1172501_sequence 1 atgaaactgaaaaacaccttgggctttgcacaccggggaagtcggcaaacagattgttgaaactgcggttttcgccgtagatgaccaccacattcttcacgttatcgtgcagggccttgtccagctcagctggcgacagttcgcgggtgactaccggcccaggcggttcagcgggcccaccgcaggcgctcagggtcgccccggcgcccagtacggcaacccctcctaaaaagcgccgacggcttgaatcggggagcgtatcggcggcattttgggtggctttcgggtcttcggtttcttcggtcatccgaggcatctcacggctgtttttggtttatttgttgctggatgtttcgttgaaaaggcatcaaatatggcaaagcttaaacgcttcagtctataaggcgaggccttagggcataagggttctgatgatgactatcgggtttcgtaacgtggccgcgctgcaatgcaccctgtgccccgtgatcgattgacggtcgatcaaggggccagaggcggtgccgggcctgagggcgaagcttaagcaggcaatgtgacagaagcgtttacggcatcacgggaggcaggtatttgagcgtggtgcctagcgcccaaagcaggaacaacaccaacggcgtgtgcaccagcagttgcacaaacgagaaaccaatcaggtctcgtgccttcagccccaacacgccgagcaacggcagcatgtagaacgggttgatcaagttcggcagcgcctcggcagcgttgtagatctgtaccgcccagcccaagtggtattgcagctcattggccacttgcatcacgtaaggcgcttcgatgatccacttgccgccccccgatggaataaagaaacccagcaccgccgagtacacgcccatcaacaacgcataggtgtcgtgagaggcgatggaggtaaagaaggtcgagatatggtgcgccagggtctggccgtcgccacccttgaccacggtcatcagtgcagcgatcgagccatacagcggaaactggatcaacacgccggtggtggtcggcacggcacgcgccaccgcatcgaggaaactgcgcgggcgccagtgcagcagtgcgccaaccatgatgaacaggaagttataggtgttcagccctgaaatggcgctgatcgccggcttggtcgaaaactcatggaataaccaacctgccgccagcaatgcaagcagaatggtcagcagcgggctgtgctccaaccattcgcccggacggcttggcgcttgaatcttgcgcaggttgaagcccaagctaagtaa BBa_K1172502_sequence 1 taatacgactcactatagggaaagaggagaaatactagatgaaactgaaaaacaccttgggctttgcacaccggggaagtcggcaaacagattgttgaaactgcggttttcgccgtagatgaccaccacattcttcacgttatcgtgcagggccttgtccagctcagctggcgacagttcgcgggtgactaccggcccaggcggttcagcgggcccaccgcaggcgctcagggtcgccccggcgcccagtacggcaacccctcctaaaaagcgccgacggcttgaatcggggagcgtatcggcggcattttgggtggctttcgggtcttcggtttcttcggtcatccgaggcatctcacggctgtttttggtttatttgttgctggatgtttcgttgaaaaggcatcaaatatggcaaagcttaaacgcttcagtctataaggcgaggccttagggcataagggttctgatgatgactatcgggtttcgtaacgtggccgcgctgcaatgcaccctgtgccccgtgatcgattgacggtcgatcaaggggccagaggcggtgccgggcctgagggcgaagcttaagcaggcaatgtgacagaagcgtttacggcatcacgggaggcaggtatttgagcgtggtgcctagcgcccaaagcaggaacaacaccaacggcgtgtgcaccagcagttgcacaaacgagaaaccaatcaggtctcgtgccttcagccccaacacgccgagcaacggcagcatgtagaacgggttgatcaagttcggcagcgcctcggcagcgttgtagatctgtaccgcccagcccaagtggtattgcagctcattggccacttgcatcacgtaaggcgcttcgatgatccacttgccgccccccgatggaataaagaaacccagcaccgccgagtacacgcccatcaacaacgcataggtgtcgtgagaggcgatggaggtaaagaaggtcgagatatggtgcgccagggtctggccgtcgccacccttgaccacggtcatcagtgcagcgatcgagccatacagcggaaactggatcaacacgccggtggtggtcggcacggcacgcgccaccgcatcgaggaaactgcgcgggcgccagtgcagcagtgcgccaaccatgatgaacaggaagttataggtgttcagccctgaaatggcgctgatcgccggcttggtcgaaaactcatggaataaccaacctgccgccagcaatgcaagcagaatggtcagcagcgggctgtgctccaaccattcgcccggacggcttggcgcttgaatcttgcgcaggttgaagcccaagctaagtaa BBa_K1172588_sequence 1 taatacgactcactatagggaaagaggagaaatactagatgaaactgaaaaacaccttgggctttgcacaccggggaagtcggcaaacagattgttgaaactgcggttttcgccgtagatgaccaccacattcttcacgttatcgtgcagggccttgtccagctcagctggcgacagttcgcgggtgactaccggcccaggcggttcagcgggcccaccgcaggcgctcagggtcgccccggcgcccagtacggcaacccctcctaaaaagcgccgacggcttgaatcggggagcgtatcggcggcattttgggtggctttcgggtcttcggtttcttcggtcatccgaggcatctcacggctgtttttggtttatttgttgctggatgtttcgttgaaaaggcatcaaatatggcaaagcttaaacgcttcagtctataaggcgaggccttagggcataagggttctgatgatgactatcgggtttcgtaacgtggccgcgctgcaatgcaccctgtgccccgtgatcgattgacggtcgatcaaggggccagaggcggtgccgggcctgagggcgaagcttaagcaggcaatgtgacagaagcgtttacggcatcacgggaggcaggtatttgagcgtggtgcctagcgcccaaagcaggaacaacaccaacggcgtgtgcaccagcagttgcacaaacgagaaaccaatcaggtctcgtgccttcagccccaacacgccgagcaacggcagcatgtagaacgggttgatcaagttcggcagcgcctcggcagcgttgtagatctgtaccgcccagcccaagtggtattgcagctcattggccacttgcatcacgtaaggcgcttcgatgatccacttgccgccccccgatggaataaagaaacccagcaccgccgagtacacgcccatcaacaacgcataggtgtcgtgagaggcgatggaggtaaagaaggtcgagatatggtgcgccagggtctggccgtcgccacccttgaccacggtcatcagtgcagcgatcgagccatacagcggaaactggatcaacacgccggtggtggtcggcacggcacgcgccaccgcatcgaggaaactgcgcgggcgccagtgcagcagtgcgccaaccatgatgaacaggaagttataggtgttcagccctgaaatggcgctgatcgccggcttggtcgaaaactcatggaataaccaacctgccgccagcaatgcaagcagaatggtcagcagcgggctgtgctccaaccattcgcccggacggcttggcgcttgaatcttgcgcaggttgaagcccaagctaagtaatactagatgaattggtcagtactcgataaccaaatgatgagccgagctattcaattggctcgcaaaggattttataccactcgccccaatcccagtgtgggctgcgttatcgttaacgataaccagattgtcggtgaaggttatcatcaaaaagccggagagccccacgctgaagtgcatgcgctgcgcatggctggcgagcttgcccgtggcgcgactgcttatgtcaccttagaaccctgtagccattatggtcgtacgccgccctgcgccttggcgctgatcaatattggtgtaaagcgggtggtggtggcggttgaagatcctaatccgcaggtcggcgggcgcggtattcaaatgctgcgcgatgcgggtattcaagtggatgtgggcttgcatcgtgatgaggcctatgccttaaatcttggttttatgaagcgcatggagtcgggtctaccttgggtgacggtaaagcttgccgcgagtcttgatggtaaaactgcgttatcaaacggggtgtctaaatggatcactggccctgaagctcgccgcgatgtgcagcgcttaagattacgatcctgcgcgctgatcacggggattgaaacggtactggccgatgatccatcgctcaatgtgcgctactcagagcttggcagtttaagagcgcaattgagtgagtcgcaggttttacagccgctgcgagtggttttggacagtcgcgcccgcatgcctatcacggcagcattgtttgcgattgaatcacccattttattagtctcgacagtgagctattcgtcagactttatggtgcaattacccgcccatgtgacttgcctccaattaccagccattgagggtcgaatctccttgtctgcactattaagctatttaggcaaaagctgtaatcaggtgcttatcgaagcgggcgcgactttagctggcgcctttatcggctcaggattagccgatgaattagtgctttatcaagcgatgaaaatccttggcggccaaggacgtaatctacttgaattacccgattatcaaatgatggccgatattccgaccctcaaattggtcgacgagcgtaaagtgggcgcggatacgcgcttcaccttgaggctcgagtccaatccatctttagcgaataagtgagttaaccatgtttactgggattattgaggccgttggtacactgcgaaagcttgagcgtaaaggcgatgatattcgcttaacggtcgccagtggcaaactggatttaaccgatgtgcgtttaggcgacagtatcgccaccaacggtgtatgtttgaccgtggttcaacagttagccgatggctatgtggctgatgtgtcagctgaaaccgtcagccttacaggttttgcaaactataaagtgggtactaaggtcaatcttgaaaaggccgtcaccccaactactcgtttaggcggccatatggtgagtggtcatgtggatgggattgctactgttgagcagcgtcttgctcgtggtcaagcaatcgaattttggttagcggctcccacggagcttgctcgctatattgcccataaaggttctatcacgattgatggcgtgagcctgacagtaaacgaagtcgatggacaccgttttcgattaacgattgtgccccatactgtaggtgaaaccacgttggtggatttaaaagcaggcgataaggtcaatattgaagtggacttaattgcccgttatttagagcgtttaatgcgttttgacagcaaagaaacccaaggcggaggggtgaccatggaaatgttagcccgtgctggctttgtgcgttagtacactggcactcagttcacttaggtatagaatttataacaacagtcacattataaaggtcctacaatggcgctgcacagtatagaagagatcatcgaggatattcgtcaaggcaaaatggttattttgatggatgacgaggacagagaaaacgaaggcgatctgattttggcggccgaaatggtgacgccagaagcgattaactttatggcgaaatacggccgcgggttaatctgccaaacgatgaccaaggcccgttgccagcagttaaatctgcccttgatggtaaccaataacaatgcgcagttctcgaccaactttacggtatcgattgaagctgccgaaggcgtaaccacagggatttcggcccacgatcgcgctgtaaccgttaaagcggccgtagcaaaagatgctaaagcgtcggacttagttcagcctgggcatatcttcccgttaatggcgcaggatggtggcgtattaacccgcgcaggtcacactgaagcgggctgtgatttagcccgtttagcagggcttgaaccatcgggggtgattgttgaaattttgaacgaagacggcactatggcccgtcgcccagatttagagattttctctgaaaaacatggcattaaaatcggcactattgcggccctgattgaataccgtaataccaaagaaacgaccgttgtgcgtgaagccaaatgcaaactgccaacccgtttcggtgagttcgatatggtgacctttagggatactatcgacaatcaattgcactttgccttagtgaaaggcgaggtgaagcctgactgtttagtacgggtacatttacagaatacctttaacgatctacttcactctgagcgtgatcagcaacgtagctggccgctcgaaaaagcgatggcgcggatttctgccgaaggtggtgtattagtactgcttggcaatcaggaacattccagtgaaatcctcgccaaagtcaaagcctttgaagcggaagatcaaggtcagaccccagtatcggctaaatggcaaggcacctcacgccgtgtaggtgtgggttcgcaaatcctcgccagtttaggcgtgacaaaaatgcgactcttaagctcgcccaaacgttatcactcactttcaggctttggccttgaagtgaccgaatgcgtagctgagtaacaggttaagcttgtaataggttgcactaaggtaaaattaaccatttctatcatttgcataaggccgcgggcaattgactgtggtatcatgtcgccacttttcgcccagagctgggtgctttagctaaattaggtaagaaaatgaacgtagttcaaggtaatatcgaagcgaagaatgccaaagttgcgattgtaatttcgcgttttaacagctttttagtcgaaagcctgcttgaaggtgcacttgacacgctgaaacgttttggccaagtcagtgatgaaaacatcactgttgtccgtgtacctggtgcggttgagttaccgctggctgcacgtcgtgttgccgcaagtggtaagtttgacggtattattgcacttggtgcggtaatccgtggtggtacccctcactttgattttgttgcaggcgaatgtaacaaaggtctggctcaaattgcattagagttcgatctgcccgttgctttcggtgtattgactacagataccattgaacaagctattgagcgttcaggtactaaagcgggtaacaaaggcggcgaagctgctctaagcttgcttgaaatggtcaacgttctgcaacagcttgaacaacagttgtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z