##gff-version 3
##sequence-region BBa_K1172588 1 4939
BBa_K1172588	.	CDS	1343	4939	.	+	0	ID=BBa_K1172303;Name=BBa_K1172303
BBa_K1172588	.	point_mutation	2986	2991	.	+	0	ID=annotation2370715;Name=Pst1 C->T;Parent=BBa_K1172303
BBa_K1172588	.	stop_codon	2486	2488	.	+	0	ID=annotation2370721;Name=tga;Parent=BBa_K1172303
BBa_K1172588	.	start_codon	1343	1345	.	+	0	ID=annotation2370717;Name=atg;Parent=BBa_K1172303
BBa_K1172588	.	stop_codon	4937	4939	.	+	0	ID=annotation2370724;Name=taa;Parent=BBa_K1172303
BBa_K1172588	.	CDS	4463	4939	.	+	0	ID=annotation2370313;Name=ribE;Parent=BBa_K1172303
BBa_K1172588	.	stop_codon	4320	4322	.	+	0	ID=annotation2370723;Name=taa;Parent=BBa_K1172303
BBa_K1172588	.	point_mutation	1576	1581	.	+	0	ID=annotation2370714;Name=Pst1 C->T;Parent=BBa_K1172303
BBa_K1172588	.	CDS	1343	2488	.	+	0	ID=annotation2370310;Name=ribD;Parent=BBa_K1172303
BBa_K1172588	.	start_codon	3219	3221	.	+	0	ID=annotation2370719;Name=atg;Parent=BBa_K1172303
BBa_K1172588	.	CDS	2496	3152	.	+	0	ID=annotation2370311;Name=SO_3468;Parent=BBa_K1172303
BBa_K1172588	.	start_codon	2496	2498	.	+	0	ID=annotation2370718;Name=atg;Parent=BBa_K1172303
BBa_K1172588	.	CDS	3219	4322	.	+	0	ID=annotation2370312;Name=ribBA;Parent=BBa_K1172303
BBa_K1172588	.	start_codon	4463	4465	.	+	0	ID=annotation2370720;Name=atg;Parent=BBa_K1172303
BBa_K1172588	.	point_mutation	3182	3187	.	+	0	ID=annotation2370716;Name=EcoR1 C->T;Parent=BBa_K1172303
BBa_K1172588	.	stop_codon	3150	3152	.	+	0	ID=annotation2370722;Name=tag;Parent=BBa_K1172303
BBa_K1172588	.	engineered_region	1	1336	.	+	0	ID=BBa_K1172502;Name=BBa_K1172502
BBa_K1172588	.	CDS	39	1336	.	+	0	ID=BBa_K1172501;Name=BBa_K1172501;Parent=BBa_K1172502
BBa_K1172588	.	stop_codon	1334	1336	.	+	0	ID=annotation2370296;Name=stop;Parent=BBa_K1172501
BBa_K1172588	.	start_codon	39	41	.	+	0	ID=annotation2370295;Name=start;Parent=BBa_K1172501
BBa_K1172588	.	CDS	39	1336	.	+	0	ID=annotation2370267;Name=cds;Parent=BBa_K1172501
BBa_K1172588	.	promoter	1	32	.	+	0	ID=BBa_K525998;Name=BBa_K525998;Parent=BBa_K1172502
BBa_K1172588	.	promoter	1	20	.	+	0	ID=annotation2129432;Name=T7 promoter;Parent=BBa_K525998
BBa_K1172588	.	engineered_region	21	32	.	+	0	ID=annotation2127788;Name=B0034;Parent=BBa_K525998
BBa_K1172588	.	ribosome_entry_site	24	27	.	+	0	ID=annotation2129433;Name=RBS;Parent=BBa_K525998
>BBa_K1172588
taatacgactcactatagggaaagaggagaaatactagatgaaactgaaaaacaccttgggctttgcacaccggggaag
tcggcaaacagattgttgaaactgcggttttcgccgtagatgaccaccacattcttcacgttatcgtgcagggccttgt
ccagctcagctggcgacagttcgcgggtgactaccggcccaggcggttcagcgggcccaccgcaggcgctcagggtcgc
cccggcgcccagtacggcaacccctcctaaaaagcgccgacggcttgaatcggggagcgtatcggcggcattttgggtg
gctttcgggtcttcggtttcttcggtcatccgaggcatctcacggctgtttttggtttatttgttgctggatgtttcgt
tgaaaaggcatcaaatatggcaaagcttaaacgcttcagtctataaggcgaggccttagggcataagggttctgatgat
gactatcgggtttcgtaacgtggccgcgctgcaatgcaccctgtgccccgtgatcgattgacggtcgatcaaggggcca
gaggcggtgccgggcctgagggcgaagcttaagcaggcaatgtgacagaagcgtttacggcatcacgggaggcaggtat
ttgagcgtggtgcctagcgcccaaagcaggaacaacaccaacggcgtgtgcaccagcagttgcacaaacgagaaaccaa
tcaggtctcgtgccttcagccccaacacgccgagcaacggcagcatgtagaacgggttgatcaagttcggcagcgcctc
ggcagcgttgtagatctgtaccgcccagcccaagtggtattgcagctcattggccacttgcatcacgtaaggcgcttcg
atgatccacttgccgccccccgatggaataaagaaacccagcaccgccgagtacacgcccatcaacaacgcataggtgt
cgtgagaggcgatggaggtaaagaaggtcgagatatggtgcgccagggtctggccgtcgccacccttgaccacggtcat
cagtgcagcgatcgagccatacagcggaaactggatcaacacgccggtggtggtcggcacggcacgcgccaccgcatcg
aggaaactgcgcgggcgccagtgcagcagtgcgccaaccatgatgaacaggaagttataggtgttcagccctgaaatgg
cgctgatcgccggcttggtcgaaaactcatggaataaccaacctgccgccagcaatgcaagcagaatggtcagcagcgg
gctgtgctccaaccattcgcccggacggcttggcgcttgaatcttgcgcaggttgaagcccaagctaagtaatactaga
tgaattggtcagtactcgataaccaaatgatgagccgagctattcaattggctcgcaaaggattttataccactcgccc
caatcccagtgtgggctgcgttatcgttaacgataaccagattgtcggtgaaggttatcatcaaaaagccggagagccc
cacgctgaagtgcatgcgctgcgcatggctggcgagcttgcccgtggcgcgactgcttatgtcaccttagaaccctgta
gccattatggtcgtacgccgccctgcgccttggcgctgatcaatattggtgtaaagcgggtggtggtggcggttgaaga
tcctaatccgcaggtcggcgggcgcggtattcaaatgctgcgcgatgcgggtattcaagtggatgtgggcttgcatcgt
gatgaggcctatgccttaaatcttggttttatgaagcgcatggagtcgggtctaccttgggtgacggtaaagcttgccg
cgagtcttgatggtaaaactgcgttatcaaacggggtgtctaaatggatcactggccctgaagctcgccgcgatgtgca
gcgcttaagattacgatcctgcgcgctgatcacggggattgaaacggtactggccgatgatccatcgctcaatgtgcgc
tactcagagcttggcagtttaagagcgcaattgagtgagtcgcaggttttacagccgctgcgagtggttttggacagtc
gcgcccgcatgcctatcacggcagcattgtttgcgattgaatcacccattttattagtctcgacagtgagctattcgtc
agactttatggtgcaattacccgcccatgtgacttgcctccaattaccagccattgagggtcgaatctccttgtctgca
ctattaagctatttaggcaaaagctgtaatcaggtgcttatcgaagcgggcgcgactttagctggcgcctttatcggct
caggattagccgatgaattagtgctttatcaagcgatgaaaatccttggcggccaaggacgtaatctacttgaattacc
cgattatcaaatgatggccgatattccgaccctcaaattggtcgacgagcgtaaagtgggcgcggatacgcgcttcacc
ttgaggctcgagtccaatccatctttagcgaataagtgagttaaccatgtttactgggattattgaggccgttggtaca
ctgcgaaagcttgagcgtaaaggcgatgatattcgcttaacggtcgccagtggcaaactggatttaaccgatgtgcgtt
taggcgacagtatcgccaccaacggtgtatgtttgaccgtggttcaacagttagccgatggctatgtggctgatgtgtc
agctgaaaccgtcagccttacaggttttgcaaactataaagtgggtactaaggtcaatcttgaaaaggccgtcacccca
actactcgtttaggcggccatatggtgagtggtcatgtggatgggattgctactgttgagcagcgtcttgctcgtggtc
aagcaatcgaattttggttagcggctcccacggagcttgctcgctatattgcccataaaggttctatcacgattgatgg
cgtgagcctgacagtaaacgaagtcgatggacaccgttttcgattaacgattgtgccccatactgtaggtgaaaccacg
ttggtggatttaaaagcaggcgataaggtcaatattgaagtggacttaattgcccgttatttagagcgtttaatgcgtt
ttgacagcaaagaaacccaaggcggaggggtgaccatggaaatgttagcccgtgctggctttgtgcgttagtacactgg
cactcagttcacttaggtatagaatttataacaacagtcacattataaaggtcctacaatggcgctgcacagtatagaa
gagatcatcgaggatattcgtcaaggcaaaatggttattttgatggatgacgaggacagagaaaacgaaggcgatctga
ttttggcggccgaaatggtgacgccagaagcgattaactttatggcgaaatacggccgcgggttaatctgccaaacgat
gaccaaggcccgttgccagcagttaaatctgcccttgatggtaaccaataacaatgcgcagttctcgaccaactttacg
gtatcgattgaagctgccgaaggcgtaaccacagggatttcggcccacgatcgcgctgtaaccgttaaagcggccgtag
caaaagatgctaaagcgtcggacttagttcagcctgggcatatcttcccgttaatggcgcaggatggtggcgtattaac
ccgcgcaggtcacactgaagcgggctgtgatttagcccgtttagcagggcttgaaccatcgggggtgattgttgaaatt
ttgaacgaagacggcactatggcccgtcgcccagatttagagattttctctgaaaaacatggcattaaaatcggcacta
ttgcggccctgattgaataccgtaataccaaagaaacgaccgttgtgcgtgaagccaaatgcaaactgccaacccgttt
cggtgagttcgatatggtgacctttagggatactatcgacaatcaattgcactttgccttagtgaaaggcgaggtgaag
cctgactgtttagtacgggtacatttacagaatacctttaacgatctacttcactctgagcgtgatcagcaacgtagct
ggccgctcgaaaaagcgatggcgcggatttctgccgaaggtggtgtattagtactgcttggcaatcaggaacattccag
tgaaatcctcgccaaagtcaaagcctttgaagcggaagatcaaggtcagaccccagtatcggctaaatggcaaggcacc
tcacgccgtgtaggtgtgggttcgcaaatcctcgccagtttaggcgtgacaaaaatgcgactcttaagctcgcccaaac
gttatcactcactttcaggctttggccttgaagtgaccgaatgcgtagctgagtaacaggttaagcttgtaataggttg
cactaaggtaaaattaaccatttctatcatttgcataaggccgcgggcaattgactgtggtatcatgtcgccacttttc
gcccagagctgggtgctttagctaaattaggtaagaaaatgaacgtagttcaaggtaatatcgaagcgaagaatgccaa
agttgcgattgtaatttcgcgttttaacagctttttagtcgaaagcctgcttgaaggtgcacttgacacgctgaaacgt
tttggccaagtcagtgatgaaaacatcactgttgtccgtgtacctggtgcggttgagttaccgctggctgcacgtcgtg
ttgccgcaagtggtaagtttgacggtattattgcacttggtgcggtaatccgtggtggtacccctcactttgattttgt
tgcaggcgaatgtaacaaaggtctggctcaaattgcattagagttcgatctgcccgttgctttcggtgtattgactaca
gataccattgaacaagctattgagcgttcaggtactaaagcgggtaacaaaggcggcgaagctgctctaagcttgcttg
aaatggtcaacgttctgcaacagcttgaacaacagttgtaa