LOCUS       BBa_K1172908            2509 bp    DNA     linear       22-Nov-2024
DEFINITION  Part 1 of the Biosafety-System Lac of Growth (pRha lacI alr)
ACCESSION   BBa_K1172908
VERSION     BBa_K1172908.1  
FEATURES             Location/Qualifiers
     promoter        1..122
                     /label=pRha (K914004)
     RBS             129..140
                     /label=B0034
     CDS             144..1271
                     /label=lacI-LVA (C0012)
     RBS             1278..1289
                     /label=B0034
     CDS             1293..2372
                     /label=Alanine-Racemase (K1172901)
     misc_feature    2381..2509
                     /label=double Terminator (B0015)
ORIGIN
        1 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
       61 attttcctgt cagtaacgag aaggtcgcgt attcaggcgc tttttagact ggtcgtaatg
      121 aatactagaa agaggagaaa caaatggtga atgtgaaacc agtaacgtta tacgatgtcg
      181 cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg
      241 tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca
      301 accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca
      361 gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac
      421 tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg
      481 cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg
      541 accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg
      601 tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg
      661 gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa
      721 gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa
      781 ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca
      841 tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg
      901 cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg
      961 tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca
     1021 aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg
     1081 gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc
     1141 tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     1201 cacgacaggt ttcccgactg gaaagcgggc aggctgcaaa cgacgaaaac tacgctttag
     1261 tagcttaata atactagaaa gaggagaaac aaatgcaagc ggcaactgtt gtgattaacc
     1321 gccgcgctct gcgacacaac ctgcaacgtc ttcgtgaact ggcccctgcc agtaaaatgg
     1381 ttgcggtggt gaaagcgaac gcttatggtc acggtcttct tgagaccgcg cgaacgctcc
     1441 ccgatgctga cgcctttggc gtagcccgtc tcgaagaagc tctgcgactg cgtgcggggg
     1501 gaatcaccaa acctgtactg ttactcgaag gcttttttga tgccagagat ctgccgacga
     1561 tttctgcgca acattttcat accgccgtgc ataacgaaga acagctggct gcgctggaag
     1621 aggctagcct ggacgagccg gttaccgtct ggatgaaact cgataccggt atgcaccgtc
     1681 tgggcgtaag gccggaacag gctgaggcgt tttatcatcg cctgacccag tgcaaaaacg
     1741 ttcgtcagcc ggtgaatatc gtcagccatt ttgcgcgcgc ggatgaacca aaatgtggcg
     1801 caaccgagaa acaactcgct atctttaata ccttttgcga aggcaaacct ggtcaacgtt
     1861 ccattgccgc gtcgggtggc attctgctgt ggccacagtc gcattttgac tgggtgcgcc
     1921 cgggcatcat tctttatggc gtctcgccgc tggaagatcg ctccaccggt gccgattttg
     1981 gctgtcagcc agtgatgtca ctaacctcca gcctgattgc cgtgcgtgag cataaagccg
     2041 gagagcctgt tggttatggt ggaacctggg taagcgaacg tgatacccgt cttggcgtag
     2101 tcgcgatggg ctatggcgat ggttatccgc gcgccgcgcc gtccggtacg ccagtgctgg
     2161 tgaacggtcg cgaagtaccg attgtcgggc gcgtggcgat ggatatgatc tgcgtagact
     2221 taggtccaca ggcgcaggac aaagccgggg atccggtcat tttatggggc gaaggtttgc
     2281 ccgtagaacg tatcgctgaa atgacgaaag taagcgctta cgaacttatt acgcgcctga
     2341 cttcaagggt cgcgatgaaa tacgtggatt aatactagag ccaggcatca aataaaacga
     2401 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     2461 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//