LOCUS BBa_K1172911 3600 bp DNA linear 25-Nov-2024 DEFINITION Biosafety-System Lac of Growth (lacI) ACCESSION BBa_K1172911 VERSION BBa_K1172911.1 FEATURES Location/Qualifiers promoter 1..122 /label=pRha (K914004) RBS 129..140 /label=B0034 CDS 144..1271 /label=lacI-LVA (C0012) RBS 1278..1289 /label=B0034 CDS 1293..2372 /label=Alanine-Racemase (K1172901) misc_feature 2381..2509 /label=double Terminator (B0015) misc_feature 1..2509 /label=BBa_K1172908 CDS 2518..2605 /label=end of LacI coding region (inactive) misc_feature 2518..2717 /label=BBa_R0010 misc_feature 2606..2643 /label=CAP binding site promoter 2654..2659 /label=-35 promoter 2678..2683 /label=-10 misc_feature 2683..2717 /label=LacI binding site misc_feature 2690..2690 /label=start promoter 2518..2717 /label=BBa_R0010 misc_feature 2730..2733 /label=conserved RBS 2726..2737 /label=BBa_B0034 CDS 2744..3463 /label=GFP protein CDS 2744..3463 /label=BBa_E0040 stem_loop 3483..3526 /label=stem_loop misc_feature 3472..3551 /label=BBa_B0010 terminator 3472..3551 /label=BBa_B0010 stem_loop 3567..3586 /label=T7 TE misc_feature 3593..3593 /label=stop polyA_site 3587..3600 /label=polya misc_feature 3560..3600 /label=BBa_B0012 terminator 3560..3600 /label=BBa_B0012 misc_feature 2726..3600 /label=BBa_I13504 misc_feature 2518..3600 /label=BBa_K741002 ORIGIN 1 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc 61 attttcctgt cagtaacgag aaggtcgcgt attcaggcgc tttttagact ggtcgtaatg 121 aatactagaa agaggagaaa caaatggtga atgtgaaacc agtaacgtta tacgatgtcg 181 cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg 241 tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca 301 accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca 361 gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac 421 tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg 481 cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg 541 accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg 601 tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg 661 gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa 721 gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa 781 ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca 841 tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg 901 cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg 961 tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca 1021 aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg 1081 gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc 1141 tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg 1201 cacgacaggt ttcccgactg gaaagcgggc aggctgcaaa cgacgaaaac tacgctttag 1261 tagcttaata atactagaaa gaggagaaac aaatgcaagc ggcaactgtt gtgattaacc 1321 gccgcgctct gcgacacaac ctgcaacgtc ttcgtgaact ggcccctgcc agtaaaatgg 1381 ttgcggtggt gaaagcgaac gcttatggtc acggtcttct tgagaccgcg cgaacgctcc 1441 ccgatgctga cgcctttggc gtagcccgtc tcgaagaagc tctgcgactg cgtgcggggg 1501 gaatcaccaa acctgtactg ttactcgaag gcttttttga tgccagagat ctgccgacga 1561 tttctgcgca acattttcat accgccgtgc ataacgaaga acagctggct gcgctggaag 1621 aggctagcct ggacgagccg gttaccgtct ggatgaaact cgataccggt atgcaccgtc 1681 tgggcgtaag gccggaacag gctgaggcgt tttatcatcg cctgacccag tgcaaaaacg 1741 ttcgtcagcc ggtgaatatc gtcagccatt ttgcgcgcgc ggatgaacca aaatgtggcg 1801 caaccgagaa acaactcgct atctttaata ccttttgcga aggcaaacct ggtcaacgtt 1861 ccattgccgc gtcgggtggc attctgctgt ggccacagtc gcattttgac tgggtgcgcc 1921 cgggcatcat tctttatggc gtctcgccgc tggaagatcg ctccaccggt gccgattttg 1981 gctgtcagcc agtgatgtca ctaacctcca gcctgattgc cgtgcgtgag cataaagccg 2041 gagagcctgt tggttatggt ggaacctggg taagcgaacg tgatacccgt cttggcgtag 2101 tcgcgatggg ctatggcgat ggttatccgc gcgccgcgcc gtccggtacg ccagtgctgg 2161 tgaacggtcg cgaagtaccg attgtcgggc gcgtggcgat ggatatgatc tgcgtagact 2221 taggtccaca ggcgcaggac aaagccgggg atccggtcat tttatggggc gaaggtttgc 2281 ccgtagaacg tatcgctgaa atgacgaaag taagcgctta cgaacttatt acgcgcctga 2341 cttcaagggt cgcgatgaaa tacgtggatt aatactagag ccaggcatca aataaaacga 2401 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 2461 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagcaa 2521 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt 2581 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt 2641 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg 2701 gataacaatt tcacacatac tagagaaaga ggagaaatac tagatgcgta aaggagaaga 2761 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa 2821 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt 2881 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta 2941 tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag 3001 tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta 3061 caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa 3121 aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa 3181 ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa 3241 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac 3301 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc 3361 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc 3421 tgctgggatt acacatggca tggatgaact atacaaataa taatactaga gccaggcatc 3481 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 3541 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //