LOCUS       BBa_K1172915            2987 bp    DNA     linear       26-Dec-2024
DEFINITION  Biosafety-System TetOR alive (TetR)
ACCESSION   BBa_K1172915
VERSION     BBa_K1172915.1  
FEATURES             Location/Qualifiers
     RBS             129..140
                     /label=B0034
     promoter        1..122
                     /label=K194004 Rhamnose promotor
     CDS             144..803
                     /label=C0040 tetR
     RBS             811..822
                     /label=B0034
     CDS             826..1905
                     /label=K1172901 alanine racemase
     misc_feature    1914..2042
                     /label=B0015 double terminator
     misc_feature    2051..2098
                     /label=R0040 tetO
     CDS             2131..2850
                     /label=E0040 GFP
     misc_feature    2859..2987
                     /label=B0015 terminator
ORIGIN
        1 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
       61 attttcctgt cagtaacgag aaggtcgcgt attcaggcgc tttttagact ggtcgtaatg
      121 aatactagaa agaggagaaa caaatgtcca gattagataa aagtaaagtg attaacagcg
      181 cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga
      241 agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg
      301 acgccttagc cattgagatg ttagataggc accatactca cttttgccct ttagaagggg
      361 aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc
      421 atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc
      481 tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg
      541 cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag
      601 tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag
      661 ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga
      721 tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtccgctgca aacgacgaaa
      781 actacgcttt agtagcttaa taagtactag aaagaggaga aacaaatgca agcggcaact
      841 gttgtgatta accgccgcgc tctgcgacac aacctgcaac gtcttcgtga actggcccct
      901 gccagtaaaa tggttgcggt ggtgaaagcg aacgcttatg gtcacggtct tcttgagacc
      961 gcgcgaacgc tccccgatgc tgacgccttt ggcgtagccc gtctcgaaga agctctgcga
     1021 ctgcgtgcgg ggggaatcac caaacctgta ctgttactcg aaggcttttt tgatgccaga
     1081 gatctgccga cgatttctgc gcaacatttt cataccgccg tgcataacga agaacagctg
     1141 gctgcgctgg aagaggctag cctggacgag ccggttaccg tctggatgaa actcgatacc
     1201 ggtatgcacc gtctgggcgt aaggccggaa caggctgagg cgttttatca tcgcctgacc
     1261 cagtgcaaaa acgttcgtca gccggtgaat atcgtcagcc attttgcgcg cgcggatgaa
     1321 ccaaaatgtg gcgcaaccga gaaacaactc gctatcttta ataccttttg cgaaggcaaa
     1381 cctggtcaac gttccattgc cgcgtcgggt ggcattctgc tgtggccaca gtcgcatttt
     1441 gactgggtgc gcccgggcat cattctttat ggcgtctcgc cgctggaaga tcgctccacc
     1501 ggtgccgatt ttggctgtca gccagtgatg tcactaacct ccagcctgat tgccgtgcgt
     1561 gagcataaag ccggagagcc tgttggttat ggtggaacct gggtaagcga acgtgatacc
     1621 cgtcttggcg tagtcgcgat gggctatggc gatggttatc cgcgcgccgc gccgtccggt
     1681 acgccagtgc tggtgaacgg tcgcgaagta ccgattgtcg ggcgcgtggc gatggatatg
     1741 atctgcgtag acttaggtcc acaggcgcag gacaaagccg gggatccggt cattttatgg
     1801 ggcgaaggtt tgcccgtaga acgtatcgct gaaatgacga aagtaagcgc ttacgaactt
     1861 attacgcgcc tgacttcaag ggtcgcgatg aaatacgtgg attaatacta gagccaggca
     1921 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     1981 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     2041 tatactagag tccctatcag tgatagagat tgacatccct atcagtgata gagatactga
     2101 gcactactag agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga
     2161 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
     2221 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
     2281 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc
     2341 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa
     2401 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct
     2461 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
     2521 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
     2581 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac
     2641 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
     2701 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
     2761 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
     2821 catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa
     2881 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     2941 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//