LOCUS BBa_K1173501 5462 bp DNA linear 05-Nov-2024 DEFINITION CcaS, CcaR, PcpcG2, cI(lambda), magenta ACCESSION BBa_K1173501 VERSION BBa_K1173501.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23104 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 1..55 /label=BBa_K608002 CDS 62..2326 /label=ccaS CDS 62..2326 /label=BBa_K592001 stem_loop 2346..2389 /label=stem_loop misc_feature 2335..2414 /label=BBa_B0010 terminator 2335..2414 /label=BBa_B0010 stem_loop 2430..2449 /label=T7 TE misc_feature 2456..2456 /label=stop polyA_site 2450..2463 /label=polya misc_feature 2423..2463 /label=BBa_B0012 terminator 2423..2463 /label=BBa_B0012 terminator 2335..2463 /label=BBa_B0015 promoter 2472..2506 /label=BBa_J23104 misc_feature 2519..2522 /label=conserved RBS 2515..2526 /label=BBa_B0034 misc_feature 2472..2526 /label=BBa_K608002 CDS 2533..3240 /label=cds CDS 2533..3240 /label=BBa_K592002 stem_loop 3260..3303 /label=stem_loop misc_feature 3249..3328 /label=BBa_B0010 terminator 3249..3328 /label=BBa_B0010 stem_loop 3344..3363 /label=T7 TE misc_feature 3370..3370 /label=stop polyA_site 3364..3377 /label=polya misc_feature 3337..3377 /label=BBa_B0012 terminator 3337..3377 /label=BBa_B0012 terminator 3249..3377 /label=BBa_B0015 promoter 3386..3623 /label=PcpcG2 promoter promoter 3386..3623 /label=BBa_K592003 misc_feature 3636..3639 /label=conserved RBS 3632..3643 /label=BBa_B0034 CDS 3653..4360 /label=cI lambda misc_feature 4361..4393 /label=LVA misc_feature 4400..4424 /label=Help:Barcodes CDS 3650..4424 /label=BBa_C0051 stem_loop 4444..4487 /label=stem_loop misc_feature 4433..4512 /label=BBa_B0010 terminator 4433..4512 /label=BBa_B0010 stem_loop 4528..4547 /label=T7 TE misc_feature 4554..4554 /label=stop polyA_site 4548..4561 /label=polya misc_feature 4521..4561 /label=BBa_B0012 terminator 4521..4561 /label=BBa_B0012 terminator 4433..4561 /label=BBa_B0015 promoter 4607..4612 /label=-10 promoter 4584..4589 /label=-35 misc_feature 4594..4610 /label=OR1 misc_feature 4570..4586 /label=OR2 misc_feature 4570..4618 /label=BBa_R0051 promoter 4570..4618 /label=BBa_R0051 misc_feature 4631..4634 /label=conserved RBS 4627..4638 /label=BBa_B0034 CDS 4645..5325 /label=eforRed CDS 4645..5325 /label=BBa_K592012 stem_loop 5345..5388 /label=stem_loop misc_feature 5334..5413 /label=BBa_B0010 terminator 5334..5413 /label=BBa_B0010 stem_loop 5429..5448 /label=T7 TE misc_feature 5455..5455 /label=stop polyA_site 5449..5462 /label=polya misc_feature 5422..5462 /label=BBa_B0012 terminator 5422..5462 /label=BBa_B0012 terminator 5334..5462 /label=BBa_B0015 ORIGIN 1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaatacta 61 gatgggcaaa tttctaattc caatcgaatt tgtttttctg gcgatcgcca tgacctgtta 121 tttatggcac agacaaaacc aagaacgccg caggattgaa attagcatca agcaacaaac 181 ccaacgggaa cgatttatta accaaattac ccaacatatc cgccaatctt taaacttgga 241 aacggtttta aataccaccg tcgctgaagt taaaaccctg ttgcaagttg atcgagttct 301 aatttatcgc atttggcaag atggcacggg cagcgccatt acggaatcgg tgaatgccaa 361 ttatcctagt attttagggc ggaccttttc cgatgaagtt tttcccgttg aataccatca 421 agcctacacc aaaggtaaag tacgggccat taatgacatt gaccaggatg acatagagat 481 ttgcctagct gatttcgtca aacaatttgg cgtgaaatca aaattagtag tgcccattct 541 tcaacataat cgtgcttctt ccctagataa tgaatcagaa tttccctatc tttgggggct 601 gttaattacc catcaatgtg cttttacccg gccatggcaa ccgtgggaag tggagttaat 661 gaaacagcta gccaatcagg tcgcgatcgc catccaacaa tcggaattat atgagcaatt 721 acagcaactc aataaagatt tggaaaaccg agtcgaaaaa cgcacccagc aacttgccgc 781 caccaatcaa tccctaagaa tggaaatcag tgagcgacaa aaaacggaag ccgctctccg 841 ccacactaac catactctgc aatccctgat tgcggcctcc cccaggggta tttttaccct 901 taatttagca gaccaaattc agatttggaa tcctacagca gaacgtattt ttggttggac 961 agaaacagaa attattgccc atccagaatt attaacatcc aacattttgc tggaagatta 1021 tcagcaattt aaacagaaag ttttatcagg catggtttcc cctagcctag aattaaaatg 1081 tcaaaaaaaa gatggtagtt ggattgaaat tgtcctttcc gctgctcccc tattggatag 1141 tgaagaaaat attgccggat tggtggcggt tgtcgccgat attaccgagc aaaagcggca 1201 ggcagaacaa attcgtttgc tacaatccgt tgtggttaat actaatgatg cggtggtgat 1261 tacggaagcg gagcccattg atgatcccgg tccgcgcatt ctctatgtca atgaagcatt 1321 tactaaaatc accggttata ctgctgaaga aatgctaggc aaaacccccc gagttttaca 1381 gggaccaaaa actagccgca ctgaattaga tagggtgcgg caagccatta gtcaatggca 1441 atcagttacc gttgaagtga ttaattatcg taaggatggc agtgagtttt gggtggaatt 1501 tagtctggtg cccgttgcca ataaaacagg tttttacacc cattggattg ctgtgcaaag 1561 ggatgtcact gagcgccgac gcacggagga agtccgccta gctttagaac gggaaaaaga 1621 attaagccgc ctaaaaactc gttttttctc catggcttcc catgaatttc gtactcccct 1681 cagtacggcc ttagctgctg cccaattact ggaaaattct gaagtggcct ggcttgatcc 1741 cgataagcgt agccggaact tacaccgtat tcaaaattcc gtgaaaaata tggtacagct 1801 cctggatgat attttaatca ttaaccgtgc cgaagcgggc aaattggaat ttaatcctaa 1861 ttggttagat ttgaaattat tgttccagca atttatcgaa gaaattcaat taagtgtcag 1921 tgaccaatat tattttgact ttatttgtag cgctcaagat acgaaggcat tggtggatga 1981 aaggttagtg cggtctattt tatctaatct gttatctaat gcgattaaat actctcccgg 2041 gggagggcag attaaaattg ccctaagcct agattcggaa cagattattt ttgaagtcac 2101 cgaccagggc attggcattt cgccagagga ccaaaagcaa atttttgaac cctttcatcg 2161 gggcaaaaat gtcagaaata ttacgggaac aggactcggt ttaatggttg ccaagaaatg 2221 tgttgactta cacagtggca gtatcttgct aaaaagtgca gttgaccagg gaacaacagt 2281 tactatctgt ttaaaacgct ataaccattt gcctcgagct taataatact agagccaggc 2341 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 2401 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 2461 atatactaga gttgacagct agctcagtcc taggtattgt gctagctact agagaaagag 2521 gagaaatact agatgcgcat tcttttagtg gaggatgatt tgccgctggc ggaaaccctt 2581 gctgaagcat tgagtgacca gctttacacc gttgatattg ccaccgacgc ttccctcgcc 2641 tgggactatg cctcccgact ggaatatgac ctcgttattt tggatgtgat gctgccggag 2701 ttggacggga ttaccctctg tcaaaaatgg cgatcgcaca gttatttaat gccaattttg 2761 atgatgacag ccagggatac gatcaatgat aaaatcacgg gcttggatgc gggggcggat 2821 gattatgtgg tcaagccagt ggatttgggg gagttatttg ccagggtgcg agctttgttg 2881 cgtcggggtt gtgcaacgtg ccaaccagtt ttagagtggg ggccaatcag gttggatcca 2941 agcacctatg aagttagtta tgacaatgag gttttgtctt tgacccgcaa ggaatacagc 3001 attctggaat tactactccg caatggccgt cgggtgctaa gtcggagcat gattatcgat 3061 agtatctgga agttggagag tcccccagag gaagatacgg ttaaggtgca tgtgcggagt 3121 ttgcgacaaa aattaaaaag tgccggttta tcagcagatg ccattgaaac ggtccatggc 3181 attgggtatc gtctggccaa tttaacggaa aaatctttgt gccaagggaa aaactaataa 3241 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 3301 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 3361 gcctttctgc gtttatatac tagagagccc attgtgcttt tctctatcaa cctcagctta 3421 cctgaagggg tgaacaggtc tgggttaatt catgttgcga aatgtaacag ttttagtcgc 3481 atcagctaac tttccgattt ctttacgatt ttctccccct tttcttcaat tttactttgt 3541 taggatcgca tttttaatgc caacacatac cagttattgg ctggacatta aacaactttt 3601 aagtttaatt actaacttta tcttactaga gaaagaggag aaatactaga tgagcacaaa 3661 aaagaaacca ttaacacaag agcagcttga ggacgcacgt cgccttaaag caatttatga 3721 aaaaaagaaa aatgaacttg gcttatccca ggaatctgtc gcagacaaga tggggatggg 3781 gcagtcaggc gttggtgctt tatttaatgg catcaatgca ttaaatgctt ataacgccgc 3841 attgcttgca aaaattctca aagttagcgt tgaagaattt agcccttcaa tcgccagaga 3901 aatctacgag atgtatgaag cggttagtat gcagccgtca cttagaagtg agtatgagta 3961 ccctgttttt tctcatgttc aggcagggat gttctcacct gagcttagaa cctttaccaa 4021 aggtgatgcg gagagatggg taagcacaac caaaaaagcc agtgattctg cattctggct 4081 tgaggttgaa ggtaattcca tgaccgcacc aacaggctcc aagccaagct ttcctgacgg 4141 aatgttaatt ctcgttgacc ctgagcaggc tgttgagcca ggtgatttct gcatagccag 4201 acttgggggt gatgagttta ccttcaagaa actgatcagg gatagcggtc aggtgttttt 4261 acaaccacta aacccacagt acccaatgat cccatgcaat gagagttgtt ccgttgtggg 4321 gaaagttatc gctagtcagt ggcctgaaga gacgtttggc gctgcaaacg acgaaaacta 4381 cgctttagta gcttaataac gctgatagtg ctagtgtaga tcgctactag agccaggcat 4441 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 4501 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 4561 atactagagt aacaccgtgc gtgttgacta ttttacctct ggcggtgata atggttgcta 4621 ctagagaaag aggagaaata ctagatgtca gtgattaagc aggtaatgaa gaccaagttg 4681 caccttgagg gcactgtcaa tggccatgat tttacgatcg agggtaaagg tgaaggcaag 4741 ccgtacgaag ggttacagca catgaaaatg acagtcacca aaggcgcgcc tctgccgttt 4801 tccgttcata ttcttacacc tagccacatg tatggaagca aaccgtttaa taagtatcca 4861 gcggatatcc cagactacca caaacagtct tttcccgaag gtatgtcttg ggagcggtcg 4921 atgatttttg aagatggtgg cgtatgcacc gccagtaatc actccagcat aaacttgcaa 4981 gagaactgtt tcatctatga tgttaaattt catggtgtga acctgcctcc ggatgggccc 5041 gtaatgcaaa aaaccattgc tggatgggag ccgagcgtgg aaacactgta cgtgcgtgac 5101 gggatgttaa aaagtgacac tgcaatggtt tttaaactga aaggaggcgg tcatcatcgt 5161 gttgatttca aaacgacgta taaagccaaa aaacctgtca agctgccaga atttcatttc 5221 gttgaacatc gcctggaact gaccaaacac gataaagatt tcacaacttg ggaccagcag 5281 gaggcagccg aaggccattt ctcaccgctg ccgaaggctc tcccatacta gagccaggca 5341 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 5401 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 5461 ta //