BBa_K1173501 1 BBa_K1173501 CcaS, CcaR, PcpcG2, cI(lambda), magenta 2013-09-19T11:00:00Z 2015-05-08T01:09:35Z Synthesised by IDT (gBlocks). Based on a variety of BioBricks. This is a composite part which is responsive to green light and controls the production of a magenta pigment. CcaS is a trans-membrane protein which responds to green light by phosphorylating CcaR when green light is present. CcaR-P then binds to a specific promoter (BBa_K592003), which allows the transcription of the cI protein. This in turn binds to a specific promoter, which prevents the transcription of a gene coding for a magenta pigment (BBa_K592012). Overall, this means that exposure of the cells to green light will halt the synthesis of magenta pigment. Inversely, lack of exposure to green light will allow the synthesis of magenta pigment. false false _1486_ 0 18234 9 It's complicated false To prevent the accidental transcription of CcaR and the magenta pigment, these genes were reverse complement-ed. This should prevent unwanted production of magneta pigment. We also had to deconstruct BBa_C0051, as we needed the cI promoter to go before the magenta pigment. false paul james component2366651 1 BBa_B0034 component2366653 1 BBa_K592012 component2366633 1 BBa_B0034 component2366607 1 BBa_K608002 component2366620 1 BBa_K608002 component2366644 1 BBa_B0015 component2366646 1 BBa_R0051 component2366660 1 BBa_B0015 component2366616 1 BBa_B0015 component2366622 1 BBa_K592002 component2366637 1 BBa_C0051 component2366631 1 BBa_K592003 component2366629 1 BBa_B0015 component2366609 1 BBa_K592001 annotation2366651 1 BBa_B0034 range2366651 1 4627 4638 annotation2366622 1 BBa_K592002 range2366622 1 2533 3240 annotation2366646 1 BBa_R0051 range2366646 1 4570 4618 annotation2366620 1 BBa_K608002 range2366620 1 2472 2526 annotation2366633 1 BBa_B0034 range2366633 1 3632 3643 annotation2366629 1 BBa_B0015 range2366629 1 3249 3377 annotation2366653 1 BBa_K592012 range2366653 1 4645 5325 annotation2366631 1 BBa_K592003 range2366631 1 3386 3623 annotation2366637 1 BBa_C0051 range2366637 1 3650 4424 annotation2366660 1 BBa_B0015 range2366660 1 5334 5462 annotation2366607 1 BBa_K608002 range2366607 1 1 55 annotation2366616 1 BBa_B0015 range2366616 1 2335 2463 annotation2366644 1 BBa_B0015 range2366644 1 4433 4561 annotation2366609 1 BBa_K592001 range2366609 1 62 2326 BBa_J23104 1 BBa_J23104 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z isolated from library of promoters replace later false false _52_ 0 483 95 In stock true N/A true John Anderson BBa_K592001 1 BBa_K592001 CcaS, green light sensor from Synechocystis sp. PCC6803 2011-09-20T11:00:00Z 2015-05-08T01:12:48Z Synechocystic sp. PCC6803 CcaS is a green light sensor protein from the genome of Synechocystis sp. PCC6803. It requires PCB as the chromophore to function. PCB is made by [[Part:BBa_I15008|ho1]] and [[Part:BBa_I15009|PcyA]]. Furthermore, CcaS works in conjunction with [[Part:BBa_K592002|CcaR]], its response regulator. This is the green light sensor used by Tabor in his multichromatic light-sensing system which composed of CcaS/CcaR and Cph8/EnvZ. false false _763_ 0 7929 9 It's complicated false This DNA was extracted from Tabor's pJT122 plasmid. Two illegal internal restriction sites had to be removed in order to allow BioBrick-compatibility. false Lei Sun annotation2136230 1 ccaS range2136230 1 1 2265 BBa_K592003 1 BBa_K592003 PcpcG2 promoter, ccaR-regulated 2011-09-16T11:00:00Z 2015-05-08T01:12:48Z This sequence can be amplified from the genome of Synechocystis PCC6803. Reference: Hirose Y, Shimada T, Narikawa R, Katayama M, Ikeuchi M (2008) Cyanobacteriochrome CcaS is the green light receptor that induces the expression of phycobilisome linker protein. Proc Natl Acad Sci U S A 105: 9528-9533. The 238 bp sequence can be directly taken from the pJT122 plasmid designed by Jeffrey Tabor. Reference: Tabor, J. J., Levskaya, A., and Voigt, C. A. (2011). Multichromatic control of gene expression in Escherichia coli. J. Mol. Biol. 405, 2, 315???324. PcpcG2 is the green-light activated promoter from the genome of Synechocystis PCC6803. This 238 bp sequence is used by Jeffrey Tabor in pJT122 plasmid and contains the entire region upstream of cpcG2 and downstream of ccaR. PcpcG2 is regulated by the light-activation of ccaS/ccaR. The gene downstream to this promoter is transcribed upon activation by ccaS/ccaR, via green light. false false _763_ 0 7929 9 Not in stock false This sequence was PCR-amplified directly from the pJT122 plasmid provided by Chris Voigt, the co-author of Tabor's paper on multichromatic light-sensing (Tabor et al. 2011). Due to its nature, optimizing primers to extract this sequence from the source plasmid was difficult. Recommend touchdown-PCR. false Lei Sun annotation2130109 1 PcpcG2 promoter range2130109 1 1 238 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_K592012 1 eforRed eforRed, red chromoprotein 2011-09-17T11:00:00Z 2015-05-08T01:12:49Z coming soon This chromoprotein eforRed naturally exhibits red color when expressed. The color is weaker than RFP, however. On agar plates and in liquid culture, the color is readily visible to naked eye in less than 24 hours of incubation. The DNA was codon-optimized for expression in E.coli and synthesized by the Korean company Bioneer Corporation. false false _763_ 0 7929 9 It's complicated false coming soon false Lei Sun annotation2131799 1 eforRed range2131799 1 1 681 BBa_K608002 1 BBa_K608002 strong Promoter and strong RBS 2011-09-14T11:00:00Z 2015-05-08T01:12:52Z assembly from iGEM parts Released HQ 2013 you can insert your part behind this part so it will be immediatly expressed in e.coli. false false _780_ 0 9115 9 In stock false cloning via 3a-assembly false Julia M??ller component2128646 1 BBa_J23104 component2128648 1 BBa_B0034 annotation2128646 1 BBa_J23104 range2128646 1 1 35 annotation2128648 1 BBa_B0034 range2128648 1 44 55 BBa_C0051 1 cI lam cI repressor from E. coli phage lambda (+LVA) 2003-01-31T12:00:00Z 2015-08-31T04:07:23Z Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Released HQ 2013 Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).</P> false false _1_ 0 24 7 In stock false References (unparsed) here: <p><a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000</P> <P><a href="http://www.genesdev.org/cgi/content/full/15/22/3013">Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. </a>Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001</P> <p></p> <P> References (unparsed) here: <p><a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000</P> <P><a href="http://www.genesdev.org/cgi/content/full/15/22/3013">Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. </a>Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001</P> <p></p> <P>BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.<P> true Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross annotation23335 1 LVA range23335 1 712 744 annotation2213991 1 Help:Barcodes range2213991 1 751 775 annotation23334 1 cI lambda range23334 1 4 711 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_R0051 1 cI lam promoter (lambda cI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z <a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Released HQ 2013 The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).</P> false true _1_ 0 24 7 In stock false <P> <P>In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (<b><font color="red">More details needed here! DE, 2/24/03</font></b>)<P> Incompatible with host expressing cI repressor. true Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross annotation2023 1 -35 range2023 1 15 20 annotation7067 1 BBa_R0051 range7067 1 1 49 annotation2022 1 -10 range2022 1 38 43 annotation2025 1 OR2 range2025 1 1 17 annotation2024 1 OR1 range2024 1 25 41 BBa_K592002 1 BBa_K592002 ccaR 2011-09-08T11:00:00Z 2015-05-08T01:12:48Z The ccaR was amplified from a pJT118 provided by Voight et al (2011) Multichromatic Control of Gene Expression in Escherichia coli, J. Mol. Biol., 405, p315???324 Released HQ 2013 ccaR is the response regulator of the green sensor that also includes the membrane-associated histidine kinase ccaS. The two-componet system is also dependent of a chromophore. false false _763_ 0 9827 9 In stock false The ccaR amplified from pJT118 was point mutated to get rid of illegal restriction site. false Erik Lundin annotation2136237 1 cds range2136237 1 1 708 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_B0034_sequence 1 aaagaggagaaa BBa_K1173501_sequence 1 ttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaatactagatgggcaaatttctaattccaatcgaatttgtttttctggcgatcgccatgacctgttatttatggcacagacaaaaccaagaacgccgcaggattgaaattagcatcaagcaacaaacccaacgggaacgatttattaaccaaattacccaacatatccgccaatctttaaacttggaaacggttttaaataccaccgtcgctgaagttaaaaccctgttgcaagttgatcgagttctaatttatcgcatttggcaagatggcacgggcagcgccattacggaatcggtgaatgccaattatcctagtattttagggcggaccttttccgatgaagtttttcccgttgaataccatcaagcctacaccaaaggtaaagtacgggccattaatgacattgaccaggatgacatagagatttgcctagctgatttcgtcaaacaatttggcgtgaaatcaaaattagtagtgcccattcttcaacataatcgtgcttcttccctagataatgaatcagaatttccctatctttgggggctgttaattacccatcaatgtgcttttacccggccatggcaaccgtgggaagtggagttaatgaaacagctagccaatcaggtcgcgatcgccatccaacaatcggaattatatgagcaattacagcaactcaataaagatttggaaaaccgagtcgaaaaacgcacccagcaacttgccgccaccaatcaatccctaagaatggaaatcagtgagcgacaaaaaacggaagccgctctccgccacactaaccatactctgcaatccctgattgcggcctcccccaggggtatttttacccttaatttagcagaccaaattcagatttggaatcctacagcagaacgtatttttggttggacagaaacagaaattattgcccatccagaattattaacatccaacattttgctggaagattatcagcaatttaaacagaaagttttatcaggcatggtttcccctagcctagaattaaaatgtcaaaaaaaagatggtagttggattgaaattgtcctttccgctgctcccctattggatagtgaagaaaatattgccggattggtggcggttgtcgccgatattaccgagcaaaagcggcaggcagaacaaattcgtttgctacaatccgttgtggttaatactaatgatgcggtggtgattacggaagcggagcccattgatgatcccggtccgcgcattctctatgtcaatgaagcatttactaaaatcaccggttatactgctgaagaaatgctaggcaaaaccccccgagttttacagggaccaaaaactagccgcactgaattagatagggtgcggcaagccattagtcaatggcaatcagttaccgttgaagtgattaattatcgtaaggatggcagtgagttttgggtggaatttagtctggtgcccgttgccaataaaacaggtttttacacccattggattgctgtgcaaagggatgtcactgagcgccgacgcacggaggaagtccgcctagctttagaacgggaaaaagaattaagccgcctaaaaactcgttttttctccatggcttcccatgaatttcgtactcccctcagtacggccttagctgctgcccaattactggaaaattctgaagtggcctggcttgatcccgataagcgtagccggaacttacaccgtattcaaaattccgtgaaaaatatggtacagctcctggatgatattttaatcattaaccgtgccgaagcgggcaaattggaatttaatcctaattggttagatttgaaattattgttccagcaatttatcgaagaaattcaattaagtgtcagtgaccaatattattttgactttatttgtagcgctcaagatacgaaggcattggtggatgaaaggttagtgcggtctattttatctaatctgttatctaatgcgattaaatactctcccgggggagggcagattaaaattgccctaagcctagattcggaacagattatttttgaagtcaccgaccagggcattggcatttcgccagaggaccaaaagcaaatttttgaaccctttcatcggggcaaaaatgtcagaaatattacgggaacaggactcggtttaatggttgccaagaaatgtgttgacttacacagtggcagtatcttgctaaaaagtgcagttgaccagggaacaacagttactatctgtttaaaacgctataaccatttgcctcgagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaatactagatgcgcattcttttagtggaggatgatttgccgctggcggaaacccttgctgaagcattgagtgaccagctttacaccgttgatattgccaccgacgcttccctcgcctgggactatgcctcccgactggaatatgacctcgttattttggatgtgatgctgccggagttggacgggattaccctctgtcaaaaatggcgatcgcacagttatttaatgccaattttgatgatgacagccagggatacgatcaatgataaaatcacgggcttggatgcgggggcggatgattatgtggtcaagccagtggatttgggggagttatttgccagggtgcgagctttgttgcgtcggggttgtgcaacgtgccaaccagttttagagtgggggccaatcaggttggatccaagcacctatgaagttagttatgacaatgaggttttgtctttgacccgcaaggaatacagcattctggaattactactccgcaatggccgtcgggtgctaagtcggagcatgattatcgatagtatctggaagttggagagtcccccagaggaagatacggttaaggtgcatgtgcggagtttgcgacaaaaattaaaaagtgccggtttatcagcagatgccattgaaacggtccatggcattgggtatcgtctggccaatttaacggaaaaatctttgtgccaagggaaaaactaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagcccattgtgcttttctctatcaacctcagcttacctgaaggggtgaacaggtctgggttaattcatgttgcgaaatgtaacagttttagtcgcatcagctaactttccgatttctttacgattttctcccccttttcttcaattttactttgttaggatcgcatttttaatgccaacacataccagttattggctggacattaaacaacttttaagtttaattactaactttatcttactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatgtcagtgattaagcaggtaatgaagaccaagttgcaccttgagggcactgtcaatggccatgattttacgatcgagggtaaaggtgaaggcaagccgtacgaagggttacagcacatgaaaatgacagtcaccaaaggcgcgcctctgccgttttccgttcatattcttacacctagccacatgtatggaagcaaaccgtttaataagtatccagcggatatcccagactaccacaaacagtcttttcccgaaggtatgtcttgggagcggtcgatgatttttgaagatggtggcgtatgcaccgccagtaatcactccagcataaacttgcaagagaactgtttcatctatgatgttaaatttcatggtgtgaacctgcctccggatgggcccgtaatgcaaaaaaccattgctggatgggagccgagcgtggaaacactgtacgtgcgtgacgggatgttaaaaagtgacactgcaatggtttttaaactgaaaggaggcggtcatcatcgtgttgatttcaaaacgacgtataaagccaaaaaacctgtcaagctgccagaatttcatttcgttgaacatcgcctggaactgaccaaacacgataaagatttcacaacttgggaccagcaggaggcagccgaaggccatttctcaccgctgccgaaggctctcccatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_R0051_sequence 1 taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc BBa_K592012_sequence 1 atgtcagtgattaagcaggtaatgaagaccaagttgcaccttgagggcactgtcaatggccatgattttacgatcgagggtaaaggtgaaggcaagccgtacgaagggttacagcacatgaaaatgacagtcaccaaaggcgcgcctctgccgttttccgttcatattcttacacctagccacatgtatggaagcaaaccgtttaataagtatccagcggatatcccagactaccacaaacagtcttttcccgaaggtatgtcttgggagcggtcgatgatttttgaagatggtggcgtatgcaccgccagtaatcactccagcataaacttgcaagagaactgtttcatctatgatgttaaatttcatggtgtgaacctgcctccggatgggcccgtaatgcaaaaaaccattgctggatgggagccgagcgtggaaacactgtacgtgcgtgacgggatgttaaaaagtgacactgcaatggtttttaaactgaaaggaggcggtcatcatcgtgttgatttcaaaacgacgtataaagccaaaaaacctgtcaagctgccagaatttcatttcgttgaacatcgcctggaactgaccaaacacgataaagatttcacaacttgggaccagcaggaggcagccgaaggccatttctcaccgctgccgaaggctctccca BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_C0051_sequence 1 atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc BBa_K592001_sequence 1 atgggcaaatttctaattccaatcgaatttgtttttctggcgatcgccatgacctgttatttatggcacagacaaaaccaagaacgccgcaggattgaaattagcatcaagcaacaaacccaacgggaacgatttattaaccaaattacccaacatatccgccaatctttaaacttggaaacggttttaaataccaccgtcgctgaagttaaaaccctgttgcaagttgatcgagttctaatttatcgcatttggcaagatggcacgggcagcgccattacggaatcggtgaatgccaattatcctagtattttagggcggaccttttccgatgaagtttttcccgttgaataccatcaagcctacaccaaaggtaaagtacgggccattaatgacattgaccaggatgacatagagatttgcctagctgatttcgtcaaacaatttggcgtgaaatcaaaattagtagtgcccattcttcaacataatcgtgcttcttccctagataatgaatcagaatttccctatctttgggggctgttaattacccatcaatgtgcttttacccggccatggcaaccgtgggaagtggagttaatgaaacagctagccaatcaggtcgcgatcgccatccaacaatcggaattatatgagcaattacagcaactcaataaagatttggaaaaccgagtcgaaaaacgcacccagcaacttgccgccaccaatcaatccctaagaatggaaatcagtgagcgacaaaaaacggaagccgctctccgccacactaaccatactctgcaatccctgattgcggcctcccccaggggtatttttacccttaatttagcagaccaaattcagatttggaatcctacagcagaacgtatttttggttggacagaaacagaaattattgcccatccagaattattaacatccaacattttgctggaagattatcagcaatttaaacagaaagttttatcaggcatggtttcccctagcctagaattaaaatgtcaaaaaaaagatggtagttggattgaaattgtcctttccgctgctcccctattggatagtgaagaaaatattgccggattggtggcggttgtcgccgatattaccgagcaaaagcggcaggcagaacaaattcgtttgctacaatccgttgtggttaatactaatgatgcggtggtgattacggaagcggagcccattgatgatcccggtccgcgcattctctatgtcaatgaagcatttactaaaatcaccggttatactgctgaagaaatgctaggcaaaaccccccgagttttacagggaccaaaaactagccgcactgaattagatagggtgcggcaagccattagtcaatggcaatcagttaccgttgaagtgattaattatcgtaaggatggcagtgagttttgggtggaatttagtctggtgcccgttgccaataaaacaggtttttacacccattggattgctgtgcaaagggatgtcactgagcgccgacgcacggaggaagtccgcctagctttagaacgggaaaaagaattaagccgcctaaaaactcgttttttctccatggcttcccatgaatttcgtactcccctcagtacggccttagctgctgcccaattactggaaaattctgaagtggcctggcttgatcccgataagcgtagccggaacttacaccgtattcaaaattccgtgaaaaatatggtacagctcctggatgatattttaatcattaaccgtgccgaagcgggcaaattggaatttaatcctaattggttagatttgaaattattgttccagcaatttatcgaagaaattcaattaagtgtcagtgaccaatattattttgactttatttgtagcgctcaagatacgaaggcattggtggatgaaaggttagtgcggtctattttatctaatctgttatctaatgcgattaaatactctcccgggggagggcagattaaaattgccctaagcctagattcggaacagattatttttgaagtcaccgaccagggcattggcatttcgccagaggaccaaaagcaaatttttgaaccctttcatcggggcaaaaatgtcagaaatattacgggaacaggactcggtttaatggttgccaagaaatgtgttgacttacacagtggcagtatcttgctaaaaagtgcagttgaccagggaacaacagttactatctgtttaaaacgctataaccatttgcctcgagcttaataa BBa_K608002_sequence 1 ttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaa BBa_K592002_sequence 1 atgcgcattcttttagtggaggatgatttgccgctggcggaaacccttgctgaagcattgagtgaccagctttacaccgttgatattgccaccgacgcttccctcgcctgggactatgcctcccgactggaatatgacctcgttattttggatgtgatgctgccggagttggacgggattaccctctgtcaaaaatggcgatcgcacagttatttaatgccaattttgatgatgacagccagggatacgatcaatgataaaatcacgggcttggatgcgggggcggatgattatgtggtcaagccagtggatttgggggagttatttgccagggtgcgagctttgttgcgtcggggttgtgcaacgtgccaaccagttttagagtgggggccaatcaggttggatccaagcacctatgaagttagttatgacaatgaggttttgtctttgacccgcaaggaatacagcattctggaattactactccgcaatggccgtcgggtgctaagtcggagcatgattatcgatagtatctggaagttggagagtcccccagaggaagatacggttaaggtgcatgtgcggagtttgcgacaaaaattaaaaagtgccggtttatcagcagatgccattgaaacggtccatggcattgggtatcgtctggccaatttaacggaaaaatctttgtgccaagggaaaaactaataa BBa_J23104_sequence 1 ttgacagctagctcagtcctaggtattgtgctagc BBa_K592003_sequence 1 agcccattgtgcttttctctatcaacctcagcttacctgaaggggtgaacaggtctgggttaattcatgttgcgaaatgtaacagttttagtcgcatcagctaactttccgatttctttacgattttctcccccttttcttcaattttactttgttaggatcgcatttttaatgccaacacataccagttattggctggacattaaacaacttttaagtttaattactaactttatct BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z