BBa_K1173501
type: ComponentDefinition
persistent identity: BBa K1173501
display id: BBa_K1173501
version: 1
was generated by: iGEM to SBOL conversion
description: CcaS, CcaR, PcpcG2, cI(lambda), magenta
created: 2013-09-19T11:00:00Z
modified: 2015-05-08T01:09:35Z
mutable provenance: Synthesised by IDT (gBlocks). Based on a variety of BioBricks.
owned by: james
owned by: myers
mutable description: This is a composite part which is responsive to green light and controls the production of a magenta pigment. CcaS is a trans-membrane protein which responds to green light by phosphorylating CcaR when green light is present. CcaR-P then binds to a specific promoter (BBa_K592003), which allows the transcription of the cI protein. This in turn binds to a specific promoter, which prevents the transcription of a gene coding for a magenta pigment (BBa_K592012). Overall, this means that exposure of the cells to green light will halt the synthesis of magenta pigment. Inversely, lack of exposure to green light will allow the synthesis of magenta pigment.
discontinued: false
dominant: false
group u list: _1486_
m user id: 0
owner id: 18234
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: To prevent the accidental transcription of CcaR and the magenta pigment, these genes were reverse complement-ed. This should prevent unwanted production of magneta pigment. We also had to deconstruct BBa_C0051, as we needed the cI promoter to go before the magenta pigment.
star: false
creator: paul james
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K608002
component: BBa_R0051
component: BBa_B0015
component: BBa_K592012
component: BBa_B0034
component: BBa_K592001
component: BBa_K592002
component: BBa_K592003
component: BBa_C0051
sequence annotation: BBa_K608002
sequence annotation: BBa_K592001
sequence annotation: BBa_B0015
sequence annotation: BBa_K592002
sequence annotation: BBa_K592003
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence annotation: BBa_R0051
sequence annotation: BBa_K592012
sequence: https://synbiohub.org/public/igem/BBa_K1173501_sequence/1

BBa_K608002
type: Component
persistent identity: component2366607
display id: component2366607
version: 1
top level: BBa_K1173501
definition: BBa_K608002
access: public

BBa_R0051
type: Component
persistent identity: component2366646
display id: component2366646
version: 1
top level: BBa_K1173501
definition: cI lam
access: public

BBa_B0015
type: Component
persistent identity: component2366644
display id: component2366644
version: 1
top level: BBa_K1173501
definition: BBa_B0015
access: public

BBa_K592012
type: Component
persistent identity: component2366653
display id: component2366653
version: 1
top level: BBa_K1173501
definition: eforRed
access: public

BBa_B0034
type: Component
persistent identity: component2366651
display id: component2366651
version: 1
top level: BBa_K1173501
definition: BBa_B0034
access: public

BBa_B0015
type: Component
persistent identity: component2366660
display id: component2366660
version: 1
top level: BBa_K1173501
definition: BBa_B0015
access: public

BBa_B0015
type: Component
persistent identity: component2366616
display id: component2366616
version: 1
top level: BBa_K1173501
definition: BBa_B0015
access: public

BBa_K592001
type: Component
persistent identity: component2366609
display id: component2366609
version: 1
top level: BBa_K1173501
definition: BBa_K592001
access: public

BBa_K592002
type: Component
persistent identity: component2366622
display id: component2366622
version: 1
top level: BBa_K1173501
definition: BBa_K592002
access: public

BBa_K608002
type: Component
persistent identity: component2366620
display id: component2366620
version: 1
top level: BBa_K1173501
definition: BBa_K608002
access: public

BBa_K592003
type: Component
persistent identity: component2366631
display id: component2366631
version: 1
top level: BBa_K1173501
definition: BBa_K592003
access: public

BBa_B0015
type: Component
persistent identity: component2366629
display id: component2366629
version: 1
top level: BBa_K1173501
definition: BBa_B0015
access: public

BBa_C0051
type: Component
persistent identity: component2366637
display id: component2366637
version: 1
top level: BBa_K1173501
definition: cI lam
access: public

BBa_B0034
type: Component
persistent identity: component2366633
display id: component2366633
version: 1
top level: BBa_K1173501
definition: BBa_B0034
access: public

BBa_K608002
type: SequenceAnnotation
persistent identity: annotation2366607
display id: annotation2366607
version: 1
top level: BBa_K1173501
location: https://synbiohub.org/public/igem/BBa_K1173501/annotation2366607/range2366607/1
component: BBa_K608002

https://synbiohub.org/public/igem/BBa_K1173501/annotation2366607/range2366607/1
type: Range
persistent identity: range2366607
display id: range2366607
version: 1
top level: BBa_K1173501
direction: direction/reverse
start: 1
end: 55
orientation: inline

BBa_K592001
type: SequenceAnnotation
persistent identity: annotation2366609
display id: annotation2366609
version: 1
top level: BBa_K1173501
location: https://synbiohub.org/public/igem/BBa_K1173501/annotation2366609/range2366609/1
component: BBa_K592001

https://synbiohub.org/public/igem/BBa_K1173501/annotation2366609/range2366609/1
type: Range
persistent identity: range2366609
display id: range2366609
version: 1
top level: BBa_K1173501
direction: direction/reverse
start: 62
end: 2326
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2366616
display id: annotation2366616
version: 1
top level: BBa_K1173501
location: https://synbiohub.org/public/igem/BBa_K1173501/annotation2366616/range2366616/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1173501/annotation2366616/range2366616/1
type: Range
persistent identity: range2366616
display id: range2366616
version: 1
top level: BBa_K1173501
direction: direction/reverse
start: 2335
end: 2463
orientation: inline

BBa_K608002
type: SequenceAnnotation
persistent identity: annotation2366620
display id: annotation2366620
version: 1
top level: BBa_K1173501
location: https://synbiohub.org/public/igem/BBa_K1173501/annotation2366620/range2366620/1
component: BBa_K608002

https://synbiohub.org/public/igem/BBa_K1173501/annotation2366620/range2366620/1
type: Range
persistent identity: range2366620
display id: range2366620
version: 1
top level: BBa_K1173501
direction: direction/reverse
start: 2472
end: 2526
orientation: inline

BBa_K592002
type: SequenceAnnotation
persistent identity: annotation2366622
display id: annotation2366622
version: 1
top level: BBa_K1173501
location: https://synbiohub.org/public/igem/BBa_K1173501/annotation2366622/range2366622/1
component: BBa_K592002

https://synbiohub.org/public/igem/BBa_K1173501/annotation2366622/range2366622/1
type: Range
persistent identity: range2366622
display id: range2366622
version: 1
top level: BBa_K1173501
direction: direction/reverse
start: 2533
end: 3240
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2366629
display id: annotation2366629
version: 1
top level: BBa_K1173501
location: https://synbiohub.org/public/igem/BBa_K1173501/annotation2366629/range2366629/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1173501/annotation2366629/range2366629/1
type: Range
persistent identity: range2366629
display id: range2366629
version: 1
top level: BBa_K1173501
direction: direction/reverse
start: 3249
end: 3377
orientation: inline

BBa_K592003
type: SequenceAnnotation
persistent identity: annotation2366631
display id: annotation2366631
version: 1
top level: BBa_K1173501
location: https://synbiohub.org/public/igem/BBa_K1173501/annotation2366631/range2366631/1
component: BBa_K592003

https://synbiohub.org/public/igem/BBa_K1173501/annotation2366631/range2366631/1
type: Range
persistent identity: range2366631
display id: range2366631
version: 1
top level: BBa_K1173501
direction: direction/reverse
start: 3386
end: 3623
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2366633
display id: annotation2366633
version: 1
top level: BBa_K1173501
location: https://synbiohub.org/public/igem/BBa_K1173501/annotation2366633/range2366633/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1173501/annotation2366633/range2366633/1
type: Range
persistent identity: range2366633
display id: range2366633
version: 1
top level: BBa_K1173501
direction: direction/reverse
start: 3632
end: 3643
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2366637
display id: annotation2366637
version: 1
top level: BBa_K1173501
location: https://synbiohub.org/public/igem/BBa_K1173501/annotation2366637/range2366637/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K1173501/annotation2366637/range2366637/1
type: Range
persistent identity: range2366637
display id: range2366637
version: 1
top level: BBa_K1173501
direction: direction/reverse
start: 3650
end: 4424
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2366644
display id: annotation2366644
version: 1
top level: BBa_K1173501
location: https://synbiohub.org/public/igem/BBa_K1173501/annotation2366644/range2366644/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1173501/annotation2366644/range2366644/1
type: Range
persistent identity: range2366644
display id: range2366644
version: 1
top level: BBa_K1173501
direction: direction/reverse
start: 4433
end: 4561
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2366646
display id: annotation2366646
version: 1
top level: BBa_K1173501
location: https://synbiohub.org/public/igem/BBa_K1173501/annotation2366646/range2366646/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_K1173501/annotation2366646/range2366646/1
type: Range
persistent identity: range2366646
display id: range2366646
version: 1
top level: BBa_K1173501
direction: direction/forward
start: 4570
end: 4618
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2366651
display id: annotation2366651
version: 1
top level: BBa_K1173501
location: https://synbiohub.org/public/igem/BBa_K1173501/annotation2366651/range2366651/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1173501/annotation2366651/range2366651/1
type: Range
persistent identity: range2366651
display id: range2366651
version: 1
top level: BBa_K1173501
direction: direction/reverse
start: 4627
end: 4638
orientation: inline

BBa_K592012
type: SequenceAnnotation
persistent identity: annotation2366653
display id: annotation2366653
version: 1
top level: BBa_K1173501
location: https://synbiohub.org/public/igem/BBa_K1173501/annotation2366653/range2366653/1
component: BBa_K592012

https://synbiohub.org/public/igem/BBa_K1173501/annotation2366653/range2366653/1
type: Range
persistent identity: range2366653
display id: range2366653
version: 1
top level: BBa_K1173501
direction: direction/reverse
start: 4645
end: 5325
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2366660
display id: annotation2366660
version: 1
top level: BBa_K1173501
location: https://synbiohub.org/public/igem/BBa_K1173501/annotation2366660/range2366660/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1173501/annotation2366660/range2366660/1
type: Range
persistent identity: range2366660
display id: range2366660
version: 1
top level: BBa_K1173501
direction: direction/reverse
start: 5334
end: 5462
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K592002
type: ComponentDefinition
persistent identity: BBa K592002
display id: BBa_K592002
version: 1
was generated by: iGEM to SBOL conversion
description: ccaR
created: 2011-09-08T11:00:00Z
modified: 2015-05-08T01:12:48Z
mutable provenance: The ccaR was amplified from a pJT118 provided by Voight et al (2011) Multichromatic Control of Gene Expression in Escherichia coli, J. Mol. Biol., 405, p315???324
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: ccaR is the response regulator of the green sensor that also includes the membrane-associated histidine kinase ccaS. The two-componet system is also dependent of a chromophore.
discontinued: false
dominant: false
experience: experience/None
group u list: _763_
m user id: 0
owner id: 9827
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The ccaR amplified from pJT118 was point mutated to get rid of illegal restriction site.
star: false
creator: Erik Lundin
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K592002_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2136237
display id: annotation2136237
version: 1
top level: BBa_K592002
location: https://synbiohub.org/public/igem/BBa_K592002/annotation2136237/range2136237/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K592002/annotation2136237/range2136237/1
type: Range
persistent identity: range2136237
display id: range2136237
version: 1
top level: BBa_K592002
direction: direction/forward
start: 1
end: 708
orientation: inline

BBa_K608002
type: ComponentDefinition
persistent identity: BBa K608002
display id: BBa_K608002
version: 1
was generated by: iGEM to SBOL conversion
description: strong Promoter and strong RBS
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:52Z
mutable provenance: assembly from iGEM parts
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: you can insert your part behind this part so it will be immediatly expressed in e.coli.
discontinued: false
dominant: false
experience: experience/None
group u list: _780_
m user id: 0
owner id: 9115
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: cloning via 3a-assembly
star: false
creator: Julia M??ller
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_J23104
sequence annotation: BBa_J23104
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K608002_sequence/1

BBa_B0034
type: Component
persistent identity: component2128648
display id: component2128648
version: 1
top level: BBa_K608002
definition: BBa_B0034
access: public

BBa_J23104
type: Component
persistent identity: component2128646
display id: component2128646
version: 1
top level: BBa_K608002
definition: BBa_J23104
access: public

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2128646
display id: annotation2128646
version: 1
top level: BBa_K608002
location: https://synbiohub.org/public/igem/BBa_K608002/annotation2128646/range2128646/1
component: BBa_J23104

https://synbiohub.org/public/igem/BBa_K608002/annotation2128646/range2128646/1
type: Range
persistent identity: range2128646
display id: range2128646
version: 1
top level: BBa_K608002
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2128648
display id: annotation2128648
version: 1
top level: BBa_K608002
location: https://synbiohub.org/public/igem/BBa_K608002/annotation2128648/range2128648/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K608002/annotation2128648/range2128648/1
type: Range
persistent identity: range2128648
display id: range2128648
version: 1
top level: BBa_K608002
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K592003
type: ComponentDefinition
persistent identity: BBa K592003
display id: BBa_K592003
version: 1
was generated by: iGEM to SBOL conversion
description: PcpcG2 promoter, ccaR-regulated
created: 2011-09-16T11:00:00Z
modified: 2015-05-08T01:12:48Z
mutable provenance: This sequence can be amplified from the genome of Synechocystis PCC6803. Reference: Hirose Y, Shimada T, Narikawa R, Katayama M, Ikeuchi M (2008) Cyanobacteriochrome CcaS is the green light receptor that induces the expression of phycobilisome linker protein. Proc Natl Acad Sci U S A 105: 9528-9533. The 238 bp sequence can be directly taken from the pJT122 plasmid designed by Jeffrey Tabor. Reference: Tabor, J. J., Levskaya, A., and Voigt, C. A. (2011). Multichromatic control of gene expression in Escherichia coli. J. Mol. Biol. 405, 2, 315???324.
owned by: james
owned by: myers
mutable description: PcpcG2 is the green-light activated promoter from the genome of Synechocystis PCC6803. This 238 bp sequence is used by Jeffrey Tabor in pJT122 plasmid and contains the entire region upstream of cpcG2 and downstream of ccaR. PcpcG2 is regulated by the light-activation of ccaS/ccaR. The gene downstream to this promoter is transcribed upon activation by ccaS/ccaR, via green light.
discontinued: false
dominant: false
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This sequence was PCR-amplified directly from the pJT122 plasmid provided by Chris Voigt, the co-author of Tabor's paper on multichromatic light-sensing (Tabor et al. 2011). Due to its nature, optimizing primers to extract this sequence from the source plasmid was difficult. Recommend touchdown-PCR.
star: false
creator: Lei Sun
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PcpcG2 promoter
sequence: https://synbiohub.org/public/igem/BBa_K592003_sequence/1

PcpcG2 promoter
type: SequenceAnnotation
persistent identity: annotation2130109
display id: annotation2130109
version: 1
top level: BBa_K592003
location: https://synbiohub.org/public/igem/BBa_K592003/annotation2130109/range2130109/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K592003/annotation2130109/range2130109/1
type: Range
persistent identity: range2130109
display id: range2130109
version: 1
top level: BBa_K592003
direction: direction/forward
start: 1
end: 238
orientation: inline

BBa_K592001
type: ComponentDefinition
persistent identity: BBa K592001
display id: BBa_K592001
version: 1
was generated by: iGEM to SBOL conversion
description: CcaS, green light sensor from Synechocystis sp. PCC6803
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:48Z
mutable provenance: Synechocystic sp. PCC6803
owned by: james
owned by: myers
mutable description: CcaS is a green light sensor protein from the genome of Synechocystis sp. PCC6803. It requires PCB as the chromophore to function. PCB is made by ho1 and PcyA. Furthermore, CcaS works in conjunction with CcaR, its response regulator. This is the green light sensor used by Tabor in his multichromatic light-sensing system which composed of CcaS/CcaR and Cph8/EnvZ.
discontinued: false
dominant: false
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This DNA was extracted from Tabor's pJT122 plasmid. Two illegal internal restriction sites had to be removed in order to allow BioBrick-compatibility.
star: false
creator: Lei Sun
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ccaS
sequence: https://synbiohub.org/public/igem/BBa_K592001_sequence/1

ccaS
type: SequenceAnnotation
persistent identity: annotation2136230
display id: annotation2136230
version: 1
top level: BBa_K592001
location: https://synbiohub.org/public/igem/BBa_K592001/annotation2136230/range2136230/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K592001/annotation2136230/range2136230/1
type: Range
persistent identity: range2136230
display id: range2136230
version: 1
top level: BBa_K592001
direction: direction/forward
start: 1
end: 2265
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

eforRed
type: ComponentDefinition
persistent identity: BBa K592012
display id: BBa_K592012
version: 1
was generated by: iGEM to SBOL conversion
description: eforRed, red chromoprotein
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:49Z
mutable provenance: coming soon
owned by: james
owned by: myers
mutable description: This chromoprotein eforRed naturally exhibits red color when expressed. The color is weaker than RFP, however. On agar plates and in liquid culture, the color is readily visible to naked eye in less than 24 hours of incubation. The DNA was codon-optimized for expression in E.coli and synthesized by the Korean company Bioneer Corporation.
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: coming soon
star: false
creator: Lei Sun
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: eforRed
sequence: https://synbiohub.org/public/igem/BBa_K592012_sequence/1

eforRed
type: SequenceAnnotation
persistent identity: annotation2131799
display id: annotation2131799
version: 1
top level: eforRed
location: https://synbiohub.org/public/igem/BBa_K592012/annotation2131799/range2131799/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K592012/annotation2131799/range2131799/1
type: Range
persistent identity: range2131799
display id: range2131799
version: 1
top level: eforRed
direction: direction/forward
start: 1
end: 681
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_J23104
type: ComponentDefinition
persistent identity: BBa J23104
display id: BBa_J23104
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
mutable description: replace later
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23104_sequence/1

https://synbiohub.org/public/igem/BBa_K1173501_sequence/1
type: Sequence
persistent identity: BBa K1173501 sequence
display id: BBa_K1173501_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaatactagatgggcaaatttctaattccaatcgaatttgtttttctggcgatcgccatgacctgttatttatggcacagacaaaaccaagaacgccgcaggattgaaattagcatcaagcaacaaacccaacgggaacgatttattaaccaaattacccaacatatccgccaatctttaaacttggaaacggttttaaataccaccgtcgctgaagttaaaaccctgttgcaagttgatcgagttctaatttatcgcatttggcaagatggcacgggcagcgccattacggaatcggtgaatgccaattatcctagtattttagggcggaccttttccgatgaagtttttcccgttgaataccatcaagcctacaccaaaggtaaagtacgggccattaatgacattgaccaggatgacatagagatttgcctagctgatttcgtcaaacaatttggcgtgaaatcaaaattagtagtgcccattcttcaacataatcgtgcttcttccctagataatgaatcagaatttccctatctttgggggctgttaattacccatcaatgtgcttttacccggccatggcaaccgtgggaagtggagttaatgaaacagctagccaatcaggtcgcgatcgccatccaacaatcggaattatatgagcaattacagcaactcaataaagatttggaaaaccgagtcgaaaaacgcacccagcaacttgccgccaccaatcaatccctaagaatggaaatcagtgagcgacaaaaaacggaagccgctctccgccacactaaccatactctgcaatccctgattgcggcctcccccaggggtatttttacccttaatttagcagaccaaattcagatttggaatcctacagcagaacgtatttttggttggacagaaacagaaattattgcccatccagaattattaacatccaacattttgctggaagattatcagcaatttaaacagaaagttttatcaggcatggtttcccctagcctagaattaaaatgtcaaaaaaaagatggtagttggattgaaattgtcctttccgctgctcccctattggatagtgaagaaaatattgccggattggtggcggttgtcgccgatattaccgagcaaaagcggcaggcagaacaaattcgtttgctacaatccgttgtggttaatactaatgatgcggtggtgattacggaagcggagcccattgatgatcccggtccgcgcattctctatgtcaatgaagcatttactaaaatcaccggttatactgctgaagaaatgctaggcaaaaccccccgagttttacagggaccaaaaactagccgcactgaattagatagggtgcggcaagccattagtcaatggcaatcagttaccgttgaagtgattaattatcgtaaggatggcagtgagttttgggtggaatttagtctggtgcccgttgccaataaaacaggtttttacacccattggattgctgtgcaaagggatgtcactgagcgccgacgcacggaggaagtccgcctagctttagaacgggaaaaagaattaagccgcctaaaaactcgttttttctccatggcttcccatgaatttcgtactcccctcagtacggccttagctgctgcccaattactggaaaattctgaagtggcctggcttgatcccgataagcgtagccggaacttacaccgtattcaaaattccgtgaaaaatatggtacagctcctggatgatattttaatcattaaccgtgccgaagcgggcaaattggaatttaatcctaattggttagatttgaaattattgttccagcaatttatcgaagaaattcaattaagtgtcagtgaccaatattattttgactttatttgtagcgctcaagatacgaaggcattggtggatgaaaggttagtgcggtctattt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592002_sequence/1
type: Sequence
persistent identity: BBa K592002 sequence
display id: BBa_K592002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgcattcttttagtggaggatgatttgccgctggcggaaacccttgctgaagcattgagtgaccagctttacaccgttgatattgccaccgacgcttccctcgcctgggactatgcctcccgactggaatatgacctcgttattttggatgtgatgctgccggagttggacgggattaccctctgtcaaaaatggcgatcgcacagttatttaatgccaattttgatgatgacagccagggatacgatcaatgataaaatcacgggcttggatgcgggggcggatgattatgtggtcaagccagtggatttgggggagttatttgccagggtgcgagctttgttgcgtcggggttgtgcaacgtgccaaccagttttagagtgggggccaatcaggttggatccaagcacctatgaagttagttatgacaatgaggttttgtctttgacccgcaaggaatacagcattctggaattactactccgcaatggccgtcgggtgctaagtcggagcatgattatcgatagtatctggaagttggagagtcccccagaggaagatacggttaaggtgcatgtgcggagtttgcgacaaaaattaaaaagtgccggtttatcagcagatgccattgaaacggtccatggcattgggtatcgtctggccaatttaacggaaaaatctttgtgccaagggaaaaactaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K608002_sequence/1
type: Sequence
persistent identity: BBa K608002 sequence
display id: BBa_K608002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592003_sequence/1
type: Sequence
persistent identity: BBa K592003 sequence
display id: BBa_K592003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agcccattgtgcttttctctatcaacctcagcttacctgaaggggtgaacaggtctgggttaattcatgttgcgaaatgtaacagttttagtcgcatcagctaactttccgatttctttacgattttctcccccttttcttcaattttactttgttaggatcgcatttttaatgccaacacataccagttattggctggacattaaacaacttttaagtttaattactaactttatct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592001_sequence/1
type: Sequence
persistent identity: BBa K592001 sequence
display id: BBa_K592001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgggcaaatttctaattccaatcgaatttgtttttctggcgatcgccatgacctgttatttatggcacagacaaaaccaagaacgccgcaggattgaaattagcatcaagcaacaaacccaacgggaacgatttattaaccaaattacccaacatatccgccaatctttaaacttggaaacggttttaaataccaccgtcgctgaagttaaaaccctgttgcaagttgatcgagttctaatttatcgcatttggcaagatggcacgggcagcgccattacggaatcggtgaatgccaattatcctagtattttagggcggaccttttccgatgaagtttttcccgttgaataccatcaagcctacaccaaaggtaaagtacgggccattaatgacattgaccaggatgacatagagatttgcctagctgatttcgtcaaacaatttggcgtgaaatcaaaattagtagtgcccattcttcaacataatcgtgcttcttccctagataatgaatcagaatttccctatctttgggggctgttaattacccatcaatgtgcttttacccggccatggcaaccgtgggaagtggagttaatgaaacagctagccaatcaggtcgcgatcgccatccaacaatcggaattatatgagcaattacagcaactcaataaagatttggaaaaccgagtcgaaaaacgcacccagcaacttgccgccaccaatcaatccctaagaatggaaatcagtgagcgacaaaaaacggaagccgctctccgccacactaaccatactctgcaatccctgattgcggcctcccccaggggtatttttacccttaatttagcagaccaaattcagatttggaatcctacagcagaacgtatttttggttggacagaaacagaaattattgcccatccagaattattaacatccaacattttgctggaagattatcagcaatttaaacagaaagttttatcaggcatggtttcccctagcctagaattaaaatgtcaaaaaaaagatggtagttggattgaaattgtcctttccgctgctcccctattggatagtgaagaaaatattgccggattggtggcggttgtcgccgatattaccgagcaaaagcggcaggcagaacaaattcgtttgctacaatccgttgtggttaatactaatgatgcggtggtgattacggaagcggagcccattgatgatcccggtccgcgcattctctatgtcaatgaagcatttactaaaatcaccggttatactgctgaagaaatgctaggcaaaaccccccgagttttacagggaccaaaaactagccgcactgaattagatagggtgcggcaagccattagtcaatggcaatcagttaccgttgaagtgattaattatcgtaaggatggcagtgagttttgggtggaatttagtctggtgcccgttgccaataaaacaggtttttacacccattggattgctgtgcaaagggatgtcactgagcgccgacgcacggaggaagtccgcctagctttagaacgggaaaaagaattaagccgcctaaaaactcgttttttctccatggcttcccatgaatttcgtactcccctcagtacggccttagctgctgcccaattactggaaaattctgaagtggcctggcttgatcccgataagcgtagccggaacttacaccgtattcaaaattccgtgaaaaatatggtacagctcctggatgatattttaatcattaaccgtgccgaagcgggcaaattggaatttaatcctaattggttagatttgaaattattgttccagcaatttatcgaagaaattcaattaagtgtcagtgaccaatattattttgactttatttgtagcgctcaagatacgaaggcattggtggatgaaaggttagtgcggtctattttatctaatctgttatctaatgcgattaaatactctcccgggggagggcagattaaaattgc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0051_sequence/1
type: Sequence
persistent identity: BBa R0051 sequence
display id: BBa_R0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592012_sequence/1
type: Sequence
persistent identity: BBa K592012 sequence
display id: BBa_K592012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtcagtgattaagcaggtaatgaagaccaagttgcaccttgagggcactgtcaatggccatgattttacgatcgagggtaaaggtgaaggcaagccgtacgaagggttacagcacatgaaaatgacagtcaccaaaggcgcgcctctgccgttttccgttcatattcttacacctagccacatgtatggaagcaaaccgtttaataagtatccagcggatatcccagactaccacaaacagtcttttcccgaaggtatgtcttgggagcggtcgatgatttttgaagatggtggcgtatgcaccgccagtaatcactccagcataaacttgcaagagaactgtttcatctatgatgttaaatttcatggtgtgaacctgcctccggatgggcccgtaatgcaaaaaaccattgctggatgggagccgagcgtggaaacactgtacgtgcgtgacgggatgttaaaaagtgacactgcaatggtttttaaactgaaaggaggcggtcatcatcgtgttgatttcaaaacgacgtataaagccaaaaaacctgtcaagctgccagaatttcatttcgttgaacatcgcctggaactgaccaaacacgataaagatttcacaacttgggaccagcaggaggcagccgaaggccatttctcaccgctgccgaaggctctccca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23104_sequence/1
type: Sequence
persistent identity: BBa J23104 sequence
display id: BBa_J23104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtattgtgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z