BBa_K1175006
1
BBa_K1175006
endo-beta-1,3-1,4 glucanase (BglS) from Bacillus Subtilis 168
2013-09-16T11:00:00Z
2015-05-08T01:09:35Z
Bacillus Subtilis 168
BglS
This gene encodes an endo-beta-1,3-1,4-glucanase (bglS), which is from the bacterium Bacillus subtilis subtilis 168. The enzyme will hydrolyze and thereby cleave internal 1,4 linkages adjacent to 1,3 linkages.
Usage and Biology
The substrates vulnerable to the bglS encoded enzyme are mixed linked beta-glucans. These glucans would have 1,3 and 1,4 beta linkages within the polysaccharide linking together the glucose monomers. Examples of these glucans can be found in oats, maize, and barley.
(1) http://mic.sgmjournals.org/content/141/2/281.long
(2) http://onlinelibrary.wiley.com/doi/10.1002/j.2050-0416.1984.tb04259.x/pdf
false
false
_1488_
0
14098
9
It's complicated
false
No design constraints were needed for RF10 insertion, a second stop codon was also added to the end of the coding region.
false
Nicholas K. Goldner
annotation2350684
1
coding region
range2350684
1
4
726
annotation2350683
1
Start Codon
range2350683
1
1
3
annotation2350746
1
Double Stop Codon
range2350746
1
727
732
BBa_K1175006_sequence
1
atgccttatctgaaacgagtgttgctgcttcttgtcactggattgtttatgagtttgtttgcagtcactgctactgcctcagctcaaacaggtggatcgttttttgacccttttaacggctataactccggtttttggcaaaaagcagatggttattcgaatggaaatatgttcaactgcacgtggcgggctaataacgtatcaatgacgtcattgggtgaaatgcgtttagcgctaacaagcccagcttataacaagtttgactgcggggaaaaccgttctgttcaaacatatggctatggactttatgaagtcagaatgaaaccagctaaaaacacagggatcgtttcatcgttcttcacttacacaggtccaacagatggaactccttgggatgagattgatatcgaatttttaggaaaagacacaacgaaggttcaatttaactattatacaaatggtgcaggaaaccatgagaagattgttgatctcgggtttgatgcagccaatgcctatcatacgtatgcattcgattggcagccaaactctattaaatggtatgtcgacgggcaattaaaacatactgcaacaaaccaaattccgacaacacctggaaagatcatgatgaacttgtggaatggcacgggtgtcgatgaatggcttggctcctacaatggtgtaaatccgctatacgctcattatgactgggtgcgctatacaaaaaaataataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z