##gff-version 3
##sequence-region BBa_K1175200 1 7951
BBa_K1175200	.	promoter	6201	6330	.	+	0	ID=BBa_K206000;Name=BBa_K206000
BBa_K1175200	.	operator	6261	6278	.	+	0	ID=annotation2049254;Name=AraI2;Parent=BBa_K206000
BBa_K1175200	.	promoter	6201	6331	.	+	0	ID=annotation2049252;Name=promoter;Parent=BBa_K206000
BBa_K1175200	.	operator	6240	6257	.	+	0	ID=annotation2049253;Name=AraI1;Parent=BBa_K206000
BBa_K1175200	.	engineered_region	1	4668	.	+	0	ID=BBa_K1175028;Name=BBa_K1175028
BBa_K1175200	.	engineered_region	139	4668	.	+	0	ID=BBa_K215104;Name=BBa_K215104;Parent=BBa_K1175028
BBa_K1175200	.	CDS	3266	4668	.	+	0	ID=BBa_K215102;Name=BBa_K215102;Parent=BBa_K215104
BBa_K1175200	.	CDS	3280	4668	.	+	0	ID=annotation2027450;Name=prtF;Parent=BBa_K215102
BBa_K1175200	.	ribosome_entry_site	3268	3279	.	+	0	ID=annotation2027449;Name=Native RBS;Parent=BBa_K215102
BBa_K1175200	.	CDS	1896	3257	.	+	0	ID=BBa_K215101;Name=BBa_K215101;Parent=BBa_K215104
BBa_K1175200	.	CDS	1911	3257	.	+	0	ID=annotation2027448;Name=prtE;Parent=BBa_K215101
BBa_K1175200	.	ribosome_entry_site	1898	1910	.	+	0	ID=annotation2027447;Name=native rbs;Parent=BBa_K215101
BBa_K1175200	.	CDS	139	1887	.	+	0	ID=BBa_K215100;Name=BBa_K215100;Parent=BBa_K215104
BBa_K1175200	.	ribosome_entry_site	141	156	.	+	0	ID=annotation2027445;Name=native RBS;Parent=BBa_K215100
BBa_K1175200	.	CDS	157	1887	.	+	0	ID=annotation2027446;Name=prtD;Parent=BBa_K215100
BBa_K1175200	.	promoter	1	130	.	+	0	ID=BBa_K206000;Name=BBa_K206000;Parent=BBa_K1175028
BBa_K1175200	.	operator	61	78	.	+	0	ID=annotation2049254;Name=AraI2;Parent=BBa_K206000
BBa_K1175200	.	promoter	1	131	.	+	0	ID=annotation2049252;Name=promoter;Parent=BBa_K206000
BBa_K1175200	.	operator	40	57	.	+	0	ID=annotation2049253;Name=AraI1;Parent=BBa_K206000
BBa_K1175200	.	ribosome_entry_site	6339	6350	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1175200	.	conserved_region	6343	6346	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1175200	.	CDS	7230	7951	.	+	0	ID=BBa_K875008;Name=BBa_K875008
BBa_K1175200	.	promoter	7230	7309	.	+	0	ID=BBa_K875002;Name=BBa_K875002;Parent=BBa_K875008
BBa_K1175200	.	ribosome_entry_site	7318	7331	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_K875008
BBa_K1175200	.	conserved_region	7324	7327	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K1175200	.	CDS	7338	7814	.	+	0	ID=BBa_K314200;Name=BBa_K314200;Parent=BBa_K875008
BBa_K1175200	.	engineered_region	7338	7814	.	+	0	ID=annotation2089456;Name=tse2;Parent=BBa_K314200
BBa_K1175200	.	terminator	7823	7951	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K875008
BBa_K1175200	.	terminator	7911	7951	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1175200	.	engineered_region	7911	7951	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1175200	.	stem_loop	7918	7937	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1175200	.	polyA_site	7938	7951	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1175200	.	stop_codon	7944	7944	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1175200	.	terminator	7823	7902	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1175200	.	engineered_region	7823	7902	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1175200	.	stem_loop	7834	7877	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1175200	.	tag	6357	7221	.	+	0	ID=BBa_K215001;Name=BBa_K215001
BBa_K1175200	.	sequence_feature	6405	6413	.	+	0	ID=annotation2027588;Name=GSA linker;Parent=BBa_K215001
BBa_K1175200	.	tag	6543	7091	.	+	0	ID=annotation2027599;Name=Secretion tag;Parent=BBa_K215001
BBa_K1175200	.	sequence_feature	6432	6440	.	+	0	ID=annotation2027590;Name=GSA linker;Parent=BBa_K215001
BBa_K1175200	.	sequence_feature	6441	6460	.	+	0	ID=annotation2027591;Name=TEV protease site;Parent=BBa_K215001
BBa_K1175200	.	tag	6357	6404	.	+	0	ID=annotation2027587;Name=nanotag;Parent=BBa_K215001
BBa_K1175200	.	tag	6516	6533	.	+	0	ID=annotation2027597;Name=His tag;Parent=BBa_K215001
BBa_K1175200	.	tag	6414	6431	.	+	0	ID=annotation2027589;Name=His tag;Parent=BBa_K215001
BBa_K1175200	.	sequence_feature	6507	6515	.	+	0	ID=annotation2027596;Name=GSA linker;Parent=BBa_K215001
BBa_K1175200	.	sequence_feature	6486	6506	.	+	0	ID=annotation2027595;Name=TEV protease site;Parent=BBa_K215001
BBa_K1175200	.	sequence_feature	6477	6485	.	+	0	ID=annotation2027594;Name=GSA linker;Parent=BBa_K215001
BBa_K1175200	.	sequence_feature	6471	6476	.	+	0	ID=annotation2027593;Name=NheI;Parent=BBa_K215001
BBa_K1175200	.	sequence_feature	6461	6470	.	+	0	ID=annotation2027592;Name=GSA linker;Parent=BBa_K215001
BBa_K1175200	.	stop_codon	7092	7221	.	+	0	ID=annotation2027600;Name=terminator (B0015);Parent=BBa_K215001
BBa_K1175200	.	sequence_feature	6534	6542	.	+	0	ID=annotation2027598;Name=GSA linker;Parent=BBa_K215001
BBa_K1175200	.	engineered_region	4677	6192	.	+	0	ID=BBa_K875004;Name=BBa_K875004
BBa_K1175200	.	tag	6036	6053	.	+	0	ID=annotation2194273;Name=tag;Parent=BBa_K875004
BBa_K1175200	.	promoter	4677	4756	.	+	0	ID=annotation2194271;Name=promoter;Parent=BBa_K875004
BBa_K1175200	.	stem_loop	6065	6192	.	+	0	ID=annotation2194270;Name=stem_loop;Parent=BBa_K875004
BBa_K1175200	.	CDS	4777	6029	.	+	0	ID=annotation2194279;Name=protein;Parent=BBa_K875004
BBa_K1175200	.	ribosome_entry_site	4765	4776	.	+	0	ID=annotation2194268;Name=rbs;Parent=BBa_K875004
>BBa_K1175200
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaggggagagagccctgccat
gaacgcgtctagcgaacgtgaccgttctctgtttggtgtgctgcgtcagttccgccgctccttttggtctgttggcatc
ttcagcgcggtcatcaatgttctgatgctggcacctagcgtctacatgctccaggtttacgaccgtgtcctcgccagcg
gcaacggtattactctgctgatgctcacgctcctgatggcgggtctgtgcgccttcatgggtgctctcgaatgggttcg
cagcctgctggtagttcgtctcggtactcgcatcgacctcgcgctcaatcaagacgtttttaacgcggctttcgcgcgt
aatctggaagctggtgatggccgtgccggtctggcgctgaccgacctcaccctgctccgtcaattcatcacgggtaatg
cactgttcgcgttcttcgacgttccgtggttcccgctctttctcctcgtactgttcctgctgcacccttggctcggcat
gctcgcactgggtggtacggttgtacctggtggcgtaggcctcgcagaaccggcgtctgaccagtctaccgcgggtggc
tctaatcaacaaagccaacaagccactcatctcgcggatgcgcagctccgtaatgcggacgttatcgaagcgatgggta
tgctcggtaacctccgtcgtcgctggctggcgcgtcactaccgctttatctccctccaaaatctcgcttccgagcgcgc
agccgccgttggtggtgcgtccaaatactctcgtattgcactccaaagcctcatgctgggcctcggtgcgctcctcgcg
atcgacggcaaaatcacgccgggtatgatgattgcgggctctatcctcgtcggtcgtgttctgtccccgattgaccagc
tgatcggtgtttggaaacagtggtcttctgcccgcattgcgtggcagcgcctgacccgtctgattgccgcatatccgcc
acgccctgcggctatggctctcccagcgccggaaggccacctgtccgttgagcaagtttctctgcgtaccgcacaaggc
aatactcgcctgcaaaacatccatttctccctgcaagcgggtgagaccctggttatcctgggcgcctctggttctggca
aatcttctctcgctcgtctgctcgtgggtgcacaatcccctactcaaggtaaagttcgcctcgatggtgcggacctgaa
ccaagttgacaaaaacaccttcggtccgaccattggttacctcccgcaggacgttcaactgttcaaaggtagcctggcg
gaaaacattgcccgtttcggcgacgccgacccggaaaaggttgttgcggcagctaagctcgccggtgttcacgaactca
tcctgtctctgcctaatggttacgacactgagctgggtgacggcggtggcggcctgtctggtggtcaacgtcagcgtat
cggtctcgcacgcgcaatgtacggcgacccgtgtctcctcattctggacgaaccgaacgcatctctggactctgagggt
gaccaagcactcatgcaggctattgtggcgctccagaaacgtggtgcaaccgttgtactcattacgcaccgtccggcac
tcaccaccctcgctcagaaaatcctgatcctccacgagggtcaacagcaacgtatgggcctggcccgtgacgttctgac
ggaactccagcagcgctccgcggcaaaccaggcgcgtatgaacccgaccgcagcaatgccacagtaataatactagaga
gcagggatctcgtgatgactggtatggatattaccactcaggacgaactcaacgaagctgccatgcgtgaccgtgcgag
ccgtgacgaagaacgtgcgctccgtctgggttggtggctcgtcctcgcgggtttcggtggtttcctcctgtgggcgctc
ctggctccactggacaagggtgttgcggttcagggtaacgttgtagtttccggtaaccgtaaagttatccagcacatgc
aaggcggtattgttgaccgcatccaagttaaggacggcgaccgtgttgccgctggtcaggttctgctcactctcaatgc
agtcgacgctcgtaccacctccgagggcctgggttctcagtacgatcagctcatcgcgcgtgaagcgcgcctcctcgcc
gagcagcgtaaccagtcttctctcgctgcaacgccacgcctgacgcaggcacgccagcgtccggaaatggcggcgatta
tcgccctccaggaagacctgctgcgctctcgtcagcagtctctcaaactggaaattgacggtgtccgtgcatctatcga
tggtctggaaacttctctgggtgctctgcaaaaagtgatgtcttctaaacagtctgaacaagcgactctgtctcaacag
ctgcaaggtctgcgtccgctggccgcagacaactacgttccgcgtaacaagatgctcgaaaccgaacgtctcttcgccc
aggtttctggcgagctggcccagacctctggtgaagttggtcgtacccgtcgtgacattcaacaacagaaactccgcat
tgcgcagcgccagcaggagtacgacaaagaagtcaactctgaactgtctgacgttcaggccaaactgaacgaagtaatc
tctcagcgtgaaaaggcggacttcaatctcgctaacgtgcaggttcgtgcgccggtggcgggcactgtggtggatatga
aaatcttcaccgaaggtggcgtaattgctccgggtcaagttatgatggacattgttccggaggatcagccactgctggt
agatggccgtatcccggttgagatggttgacaaagtatggagcggtctgccggtcgaactgcaatttactgcgttttcc
caatctactacgccgcgtgtccctggtactgtaaccctgctgagcgcggatcgcctggtggacgaaaaagatggcaccc
cttactatggcctccgtattcaggtgtctgaggaaggtaaacgtagcctccacggcctggagattaaaccgggtatgcc
tgtccaaggttttgtgcgtacgggcgaacgctctttcatcaactacctcttcaaaccgctcatggaccgtatgcacctc
gcactgaccgaagaataatactagagagcggaaggaagcaatgcgccgtaaagcagttctgctcactgtggttctgagc
ctgtctggtggtagcgcccaagcgatgggtctgctggacgcgtgggaactcgcgctccgcaatgatgcgcaactgcgtg
cggcaggtttcgaacgtgacgcgggtcaagaggaagttgcgattggtcgcgcaggcctcctgccgagcctgcaatacac
gtacggtgcgaactactcccactccaaagttacccagcgcgatcgtaccctgaacaacaccacgaaacgtgattatgat
aactacgtttctacgctgaccctgcgtcagccactcctggactacgcggcatgggcgcgttatcagcaaggcgtaactc
gtaaactgatggcggaccagcgttttcgcgaccgttcccaggatctgatggttcgtctgtaccagtcctggagcgaagc
cctgctggcgcaggaaaagctgatgctcctcgacgctcagcgtcgtgcataccaggaacagctcgcactgaatcgccgt
ctcctggctgccggtgagggcacgcaaaccgacctgcgtgagaccgaagcacgttataccgtcaccgaggcgcagcgta
tcgaacaggaggacaccctcgatgccgccatgactgacctggaaaacatgatgggctctcctctccagatccaagacct
gtctccactggctctcgacactctcccggataacgtaacggaaaaccgtagcctctctcagtggcgtgaactcacggta
cgtcacaacgccaaactggcagtacaacgtgagaacgtggattactctcgctacgaaatcgagcgcaaccgtgcgggcc
acctcccgaccctggacctggttgcctctacccgtaattccctctccgagagcgaatacaactacaatcaaaaatacga
cacccagaccgtaggtctgcaagttcgtgttcctctctactctggcggtgcggtttctgcgtctatgcgccaagcggca
gctgagtatcaacagtcccaggcggaactggacaatcagactcgtcagaccttcgcggagctgcgtcgccagttcaacc
tgtgcgcaaacggtgctgcgaaaatccgtgcttggcagatgtctgtagctgctgccgaagaggccattcgtgcgacccg
tcagtctgttgcgggtggtgaacgtattaacctggatgtgctcatggctgagcaggaatggtataatgcccgtcgtgag
ctgacggaggttaagtaccgttggctgcaagcgtggctcaatctgcgctacacggctggtactctcaacgaacaagata
tgatgcagctggcagcctggttccagtctgcgccggttatcaataaaaccggcatcaatgctgccacgggcaacaagac
caattaatactagagaatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaattgtgagcgg
ataacaatttcacacatactagagaaagaggagaaatactagaaagaggagaaatactaatgaaagctactaaactggt
actgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccg
tatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaag
ctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcat
ggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggt
gttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacacca
tcggcactcgtccggacaacggcggaggttctggagatattctgctgacccagagcccggcgattctgagcgtgagccc
gggcgaacgcgtgagctttagctgccgcgcgagccagagcattggcgtgctgtgcgatcgcattagcattcattggtat
cagcagcgcaccaacggcagcccgcgcctgctgattaaatatgcgagcgaaagcattagcggcattccgagccgcttta
gcggcagcggcagcggcaccgattttaccctgagcattaacagcgtggaaagcgaagatattgcggattattattgcca
gcagagcaacagctggccgtggacctttggcggcggcaccaaactggaaattaaaggcggcaaaggcagcggcggcaaa
ggcaccggcggcaaaggcagcggcggcaaaggcagcgaagtgaaactggtggaaagcggcggcgatctggtgcagccgg
gcggcagcctgaaactgagctgcgcggtgcattgcgatcgcaccagcggctttacctttagcgattattatatgtattg
ggtgcgccagaccccggaaaaacgcctggaatgggtggcgtatattagcagcgtgcattgcgatcgcggcggcggcagc
accttttatccagataccgtgaaaggccgctttaccattagccgcgataacgcgaaaaacaccctgtatctacagatga
gccgcctgaaaagcgatgataccgcgatgtattattgcgcgcgcgcgtatcatggcaactattttgattattggggcca
gggcaccaccctgaccgtgagcagctccggtcaccaccaccaccaccactgatactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgct
caccttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacactttgctatgccata
gcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgttttt
ttgggctagctactagagaaagaggagaaatactagatggacgttgaagcatggctgggtgcccgtgtaccgctggttg
aaacgggcagcgcacatcaccaccatcatcatggttccgccgaaaacctctactttcagggcggctctgctgctagcgg
ttctgccgagaatctgtacttccaaggtggctccgctcatcaccaccatcatcacggtagcgcaggcggcactgacacc
tttgacttctctggttactctaacaatcagcgtatcaacctgaacgagggttctttctctgacgttggtggtctgaagg
gcaacgtttctatcgcgcatggtgttaccatcgaaaacgcgatcggtggttctggtaacgacatcctgatcggtaatgg
cgcggataacatcctccagggtggtgcgggtgacgacgttctgtacggttctacgggtgcggacacgctgacgggtggc
gctggtcgtgacattttcgtatatggtagcggtcaagactctaccgtttctgcatacgactggatcacggattttcaga
cgggtatcgacaaaatcgacctgtccgctttccgtaatgaaggtcagctgtcttttgttcaggaccagtttactggtaa
aggccaggaggttatgctgcaatgggacgcggcgaactctacgaccaatctgtggctgcacgaagcgggtcactcttct
gttgactttctcgttcgtatcgttggccagaccgcgcagagcgacattatcgtctaataaccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttatattactagagaatcataaaaaatttatttgctttgtgagcggataacaa
ttataatagattcaattgtgagcggataacaatttcacacatactagagtcacacaggaaacctactagatgtcctacg
actacgagaaaaccagcctcaccctctaccgggcggtattcaaggccaactacgacggcgacgtcggtcgctacctgca
tcccgacaaggaactcgccgaggctgcggaagtcgccccgctgctgcatccgaccttcgacagccccaacacccctggc
gtccccgcccgcgcgccggacatcgtcgccggccgcgacggcctctacgccccggacaccggcggcacctcggtgttcg
accgcgccggcgtgctgcgccgcgccgacggcgacttcgtgatacccgacggcaccgacatcccgccggaccttaaggt
gaagcaggacagctacaacaagcgcctgcaagccacccactacaccatcatgccggccaagccgatgtaccgggaggtc
ctcatgggccaactggacaacttcgtgcgcaacgccatccgccgccaatgggaaaaagcccgcgggctctagtactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata