LOCUS BBa_K1175300 9952 bp DNA linear 22-Dec-2024 DEFINITION the whole magilua ACCESSION BBa_K1175300 VERSION BBa_K1175300.1 FEATURES Location/Qualifiers promoter 1..131 /label=promoter misc_feature 40..57 /label=AraI1 misc_feature 61..78 /label=AraI2 promoter 1..130 /label=BBa_K206000 RBS 141..156 /label=native RBS CDS 157..1887 /label=prtD CDS 139..1887 /label=BBa_K215100 RBS 1898..1910 /label=native rbs CDS 1911..3257 /label=prtE CDS 1896..3257 /label=BBa_K215101 RBS 3268..3279 /label=Native RBS CDS 3280..4668 /label=prtF CDS 3266..4668 /label=BBa_K215102 misc_feature 139..4668 /label=BBa_K215104 misc_feature 1..4668 /label=BBa_K1175028 RBS 4765..4776 /label=rbs stem_loop 6065..6192 /label=stem_loop promoter 4677..4756 /label=promoter misc_feature 6036..6053 /label=tag CDS 4777..6029 /label=protein misc_feature 4677..6192 /label=BBa_K875004 promoter 6201..6331 /label=promoter misc_feature 6240..6257 /label=AraI1 misc_feature 6261..6278 /label=AraI2 promoter 6201..6330 /label=BBa_K206000 misc_feature 6343..6346 /label=conserved RBS 6339..6350 /label=BBa_B0034 misc_feature 6357..6404 /label=nanotag misc_feature 6405..6413 /label=GSA linker misc_feature 6414..6431 /label=His tag misc_feature 6432..6440 /label=GSA linker misc_feature 6441..6460 /label=TEV protease site misc_feature 6461..6470 /label=GSA linker misc_feature 6471..6476 /label=NheI misc_feature 6477..6485 /label=GSA linker misc_feature 6486..6506 /label=TEV protease site misc_feature 6507..6515 /label=GSA linker misc_feature 6516..6533 /label=His tag misc_feature 6534..6542 /label=GSA linker misc_feature 6543..7091 /label=Secretion tag misc_feature 7092..7221 /label=terminator (B0015) misc_feature 6357..7221 /label=BBa_K215001 misc_feature 7228..7230 /label=Start Codon CDS 7231..9215 /label=YesZ Enzyme misc_feature 9216..9222 /label=Double Stop Codon CDS 7228..9222 /label=BBa_K1175007 promoter 9231..9310 /label=BBa_K875002 misc_feature 9325..9328 /label=conserved RBS 9319..9332 /label=BBa_B0031 misc_feature 9339..9815 /label=tse2 CDS 9339..9815 /label=BBa_K314200 stem_loop 9835..9878 /label=stem_loop misc_feature 9824..9903 /label=BBa_B0010 terminator 9824..9903 /label=BBa_B0010 stem_loop 9919..9938 /label=T7 TE misc_feature 9945..9945 /label=stop polyA_site 9939..9952 /label=polya misc_feature 9912..9952 /label=BBa_B0012 terminator 9912..9952 /label=BBa_B0012 terminator 9824..9952 /label=BBa_B0015 CDS 9231..9952 /label=BBa_K875008 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagag gggagagagc cctgccatga acgcgtctag cgaacgtgac 181 cgttctctgt ttggtgtgct gcgtcagttc cgccgctcct tttggtctgt tggcatcttc 241 agcgcggtca tcaatgttct gatgctggca cctagcgtct acatgctcca ggtttacgac 301 cgtgtcctcg ccagcggcaa cggtattact ctgctgatgc tcacgctcct gatggcgggt 361 ctgtgcgcct tcatgggtgc tctcgaatgg gttcgcagcc tgctggtagt tcgtctcggt 421 actcgcatcg acctcgcgct caatcaagac gtttttaacg cggctttcgc gcgtaatctg 481 gaagctggtg atggccgtgc cggtctggcg ctgaccgacc tcaccctgct ccgtcaattc 541 atcacgggta atgcactgtt cgcgttcttc gacgttccgt ggttcccgct ctttctcctc 601 gtactgttcc tgctgcaccc ttggctcggc atgctcgcac tgggtggtac ggttgtacct 661 ggtggcgtag gcctcgcaga accggcgtct gaccagtcta ccgcgggtgg ctctaatcaa 721 caaagccaac aagccactca tctcgcggat gcgcagctcc gtaatgcgga cgttatcgaa 781 gcgatgggta tgctcggtaa cctccgtcgt cgctggctgg cgcgtcacta ccgctttatc 841 tccctccaaa atctcgcttc cgagcgcgca gccgccgttg gtggtgcgtc caaatactct 901 cgtattgcac tccaaagcct catgctgggc ctcggtgcgc tcctcgcgat cgacggcaaa 961 atcacgccgg gtatgatgat tgcgggctct atcctcgtcg gtcgtgttct gtccccgatt 1021 gaccagctga tcggtgtttg gaaacagtgg tcttctgccc gcattgcgtg gcagcgcctg 1081 acccgtctga ttgccgcata tccgccacgc cctgcggcta tggctctccc agcgccggaa 1141 ggccacctgt ccgttgagca agtttctctg cgtaccgcac aaggcaatac tcgcctgcaa 1201 aacatccatt tctccctgca agcgggtgag accctggtta tcctgggcgc ctctggttct 1261 ggcaaatctt ctctcgctcg tctgctcgtg ggtgcacaat cccctactca aggtaaagtt 1321 cgcctcgatg gtgcggacct gaaccaagtt gacaaaaaca ccttcggtcc gaccattggt 1381 tacctcccgc aggacgttca actgttcaaa ggtagcctgg cggaaaacat tgcccgtttc 1441 ggcgacgccg acccggaaaa ggttgttgcg gcagctaagc tcgccggtgt tcacgaactc 1501 atcctgtctc tgcctaatgg ttacgacact gagctgggtg acggcggtgg cggcctgtct 1561 ggtggtcaac gtcagcgtat cggtctcgca cgcgcaatgt acggcgaccc gtgtctcctc 1621 attctggacg aaccgaacgc atctctggac tctgagggtg accaagcact catgcaggct 1681 attgtggcgc tccagaaacg tggtgcaacc gttgtactca ttacgcaccg tccggcactc 1741 accaccctcg ctcagaaaat cctgatcctc cacgagggtc aacagcaacg tatgggcctg 1801 gcccgtgacg ttctgacgga actccagcag cgctccgcgg caaaccaggc gcgtatgaac 1861 ccgaccgcag caatgccaca gtaataatac tagagagcag ggatctcgtg atgactggta 1921 tggatattac cactcaggac gaactcaacg aagctgccat gcgtgaccgt gcgagccgtg 1981 acgaagaacg tgcgctccgt ctgggttggt ggctcgtcct cgcgggtttc ggtggtttcc 2041 tcctgtgggc gctcctggct ccactggaca agggtgttgc ggttcagggt aacgttgtag 2101 tttccggtaa ccgtaaagtt atccagcaca tgcaaggcgg tattgttgac cgcatccaag 2161 ttaaggacgg cgaccgtgtt gccgctggtc aggttctgct cactctcaat gcagtcgacg 2221 ctcgtaccac ctccgagggc ctgggttctc agtacgatca gctcatcgcg cgtgaagcgc 2281 gcctcctcgc cgagcagcgt aaccagtctt ctctcgctgc aacgccacgc ctgacgcagg 2341 cacgccagcg tccggaaatg gcggcgatta tcgccctcca ggaagacctg ctgcgctctc 2401 gtcagcagtc tctcaaactg gaaattgacg gtgtccgtgc atctatcgat ggtctggaaa 2461 cttctctggg tgctctgcaa aaagtgatgt cttctaaaca gtctgaacaa gcgactctgt 2521 ctcaacagct gcaaggtctg cgtccgctgg ccgcagacaa ctacgttccg cgtaacaaga 2581 tgctcgaaac cgaacgtctc ttcgcccagg tttctggcga gctggcccag acctctggtg 2641 aagttggtcg tacccgtcgt gacattcaac aacagaaact ccgcattgcg cagcgccagc 2701 aggagtacga caaagaagtc aactctgaac tgtctgacgt tcaggccaaa ctgaacgaag 2761 taatctctca gcgtgaaaag gcggacttca atctcgctaa cgtgcaggtt cgtgcgccgg 2821 tggcgggcac tgtggtggat atgaaaatct tcaccgaagg tggcgtaatt gctccgggtc 2881 aagttatgat ggacattgtt ccggaggatc agccactgct ggtagatggc cgtatcccgg 2941 ttgagatggt tgacaaagta tggagcggtc tgccggtcga actgcaattt actgcgtttt 3001 cccaatctac tacgccgcgt gtccctggta ctgtaaccct gctgagcgcg gatcgcctgg 3061 tggacgaaaa agatggcacc ccttactatg gcctccgtat tcaggtgtct gaggaaggta 3121 aacgtagcct ccacggcctg gagattaaac cgggtatgcc tgtccaaggt tttgtgcgta 3181 cgggcgaacg ctctttcatc aactacctct tcaaaccgct catggaccgt atgcacctcg 3241 cactgaccga agaataatac tagagagcgg aaggaagcaa tgcgccgtaa agcagttctg 3301 ctcactgtgg ttctgagcct gtctggtggt agcgcccaag cgatgggtct gctggacgcg 3361 tgggaactcg cgctccgcaa tgatgcgcaa ctgcgtgcgg caggtttcga acgtgacgcg 3421 ggtcaagagg aagttgcgat tggtcgcgca ggcctcctgc cgagcctgca atacacgtac 3481 ggtgcgaact actcccactc caaagttacc cagcgcgatc gtaccctgaa caacaccacg 3541 aaacgtgatt atgataacta cgtttctacg ctgaccctgc gtcagccact cctggactac 3601 gcggcatggg cgcgttatca gcaaggcgta actcgtaaac tgatggcgga ccagcgtttt 3661 cgcgaccgtt cccaggatct gatggttcgt ctgtaccagt cctggagcga agccctgctg 3721 gcgcaggaaa agctgatgct cctcgacgct cagcgtcgtg cataccagga acagctcgca 3781 ctgaatcgcc gtctcctggc tgccggtgag ggcacgcaaa ccgacctgcg tgagaccgaa 3841 gcacgttata ccgtcaccga ggcgcagcgt atcgaacagg aggacaccct cgatgccgcc 3901 atgactgacc tggaaaacat gatgggctct cctctccaga tccaagacct gtctccactg 3961 gctctcgaca ctctcccgga taacgtaacg gaaaaccgta gcctctctca gtggcgtgaa 4021 ctcacggtac gtcacaacgc caaactggca gtacaacgtg agaacgtgga ttactctcgc 4081 tacgaaatcg agcgcaaccg tgcgggccac ctcccgaccc tggacctggt tgcctctacc 4141 cgtaattccc tctccgagag cgaatacaac tacaatcaaa aatacgacac ccagaccgta 4201 ggtctgcaag ttcgtgttcc tctctactct ggcggtgcgg tttctgcgtc tatgcgccaa 4261 gcggcagctg agtatcaaca gtcccaggcg gaactggaca atcagactcg tcagaccttc 4321 gcggagctgc gtcgccagtt caacctgtgc gcaaacggtg ctgcgaaaat ccgtgcttgg 4381 cagatgtctg tagctgctgc cgaagaggcc attcgtgcga cccgtcagtc tgttgcgggt 4441 ggtgaacgta ttaacctgga tgtgctcatg gctgagcagg aatggtataa tgcccgtcgt 4501 gagctgacgg aggttaagta ccgttggctg caagcgtggc tcaatctgcg ctacacggct 4561 ggtactctca acgaacaaga tatgatgcag ctggcagcct ggttccagtc tgcgccggtt 4621 atcaataaaa ccggcatcaa tgctgccacg ggcaacaaga ccaattaata ctagagaatc 4681 ataaaaaatt tatttgcttt gtgagcggat aacaattata atagattcaa ttgtgagcgg 4741 ataacaattt cacacatact agagaaagag gagaaatact agaaagagga gaaatactaa 4801 tgaaagctac taaactggta ctgggcgcgg taatcctggg ttctactctg ctggcaggtt 4861 gctccagcaa cgctaaaatc gatcagggaa ttaacccgta tgttggcttt gaaatgggtt 4921 acgactggtt aggtcgtatg ccgtacaaag gcagcgttga aaacggtgca tacaaagctc 4981 agggcgttca actgaccgct aaactgggtt acccaatcac tgacgacctg gacatctaca 5041 ctcgtctggg tggcatggta tggcgtgcag acactaaatc caacgtttat ggtaaaaacc 5101 acgacaccgg cgtttctccg gtcttcgctg gcggtgttga gtacgcgatc actcctgaaa 5161 tcgctacccg tctggaatac cagtggacca acaacatcgg tgacgcacac accatcggca 5221 ctcgtccgga caacggcgga ggttctggag atattctgct gacccagagc ccggcgattc 5281 tgagcgtgag cccgggcgaa cgcgtgagct ttagctgccg cgcgagccag agcattggcg 5341 tgctgtgcga tcgcattagc attcattggt atcagcagcg caccaacggc agcccgcgcc 5401 tgctgattaa atatgcgagc gaaagcatta gcggcattcc gagccgcttt agcggcagcg 5461 gcagcggcac cgattttacc ctgagcatta acagcgtgga aagcgaagat attgcggatt 5521 attattgcca gcagagcaac agctggccgt ggacctttgg cggcggcacc aaactggaaa 5581 ttaaaggcgg caaaggcagc ggcggcaaag gcaccggcgg caaaggcagc ggcggcaaag 5641 gcagcgaagt gaaactggtg gaaagcggcg gcgatctggt gcagccgggc ggcagcctga 5701 aactgagctg cgcggtgcat tgcgatcgca ccagcggctt tacctttagc gattattata 5761 tgtattgggt gcgccagacc ccggaaaaac gcctggaatg ggtggcgtat attagcagcg 5821 tgcattgcga tcgcggcggc ggcagcacct tttatccaga taccgtgaaa ggccgcttta 5881 ccattagccg cgataacgcg aaaaacaccc tgtatctaca gatgagccgc ctgaaaagcg 5941 atgataccgc gatgtattat tgcgcgcgcg cgtatcatgg caactatttt gattattggg 6001 gccagggcac caccctgacc gtgagcagct ccggtcacca ccaccaccac cactgatact 6061 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 6121 tgttgtttgt cggtgaacgc tctctactag agtcacactg ctcaccttcg ggtgggcctt 6181 tctgcgttta tatactagag acattgatta tttgcacggc gtcacacttt gctatgccat 6241 agcaagatag tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac 6301 tgtttctcca taccgttttt ttgggctagc tactagagaa agaggagaaa tactagatgg 6361 acgttgaagc atggctgggt gcccgtgtac cgctggttga aacgggcagc gcacatcacc 6421 accatcatca tggttccgcc gaaaacctct actttcaggg cggctctgct gctagcggtt 6481 ctgccgagaa tctgtacttc caaggtggct ccgctcatca ccaccatcat cacggtagcg 6541 caggcggcac tgacaccttt gacttctctg gttactctaa caatcagcgt atcaacctga 6601 acgagggttc tttctctgac gttggtggtc tgaagggcaa cgtttctatc gcgcatggtg 6661 ttaccatcga aaacgcgatc ggtggttctg gtaacgacat cctgatcggt aatggcgcgg 6721 ataacatcct ccagggtggt gcgggtgacg acgttctgta cggttctacg ggtgcggaca 6781 cgctgacggg tggcgctggt cgtgacattt tcgtatatgg tagcggtcaa gactctaccg 6841 tttctgcata cgactggatc acggattttc agacgggtat cgacaaaatc gacctgtccg 6901 ctttccgtaa tgaaggtcag ctgtcttttg ttcaggacca gtttactggt aaaggccagg 6961 aggttatgct gcaatgggac gcggcgaact ctacgaccaa tctgtggctg cacgaagcgg 7021 gtcactcttc tgttgacttt ctcgttcgta tcgttggcca gaccgcgcag agcgacatta 7081 tcgtctaata accaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 7141 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 7201 tgggcctttc tgcgtttata ttactagatg agaaaactgt atcatggcgc ttgctattat 7261 ccggaattat gggatgaaga gacgattcag caggacattg acatcatgcg tgaagttggc 7321 gtaaatgttg tgcggatcgg cgaatttgcc tggtcagtca tggaacctga agaaggaaaa 7381 attgacgtcg gttttttcaa agaaatcatc gcccggctgt atgatagcgg gatcgaaacg 7441 attatgtgca cgccgacgcc taccccgccg atttggttct cacatggccg gcccgaacgc 7501 atgcatgcca atgaaaaaag agagatcatg gggcatggct cccgtcagca tgcctgtacg 7561 aacaacccgt atttccgaaa aaaagccgcc atcatcacca cagccatcgc caaggagctt 7621 ggccggctcc cggggctgat cggatggcag ctagacaatg agtttaaatg ccatgttgca 7681 gaatgcatgt gtgagacatg cttgcgccta tggcatgact ggctcaaaaa tcgctacggg 7741 gtaattgagc gcttgaatga agcttgggga accgatgtgt ggagcgagac ctatcagacg 7801 tttgagcaag tcccgcagcc gggaccggcc ccgtttctgc atcatgcctc tctacgcact 7861 atgtatcagc tgttttcgat ggagatgatc gcttcgtttg cggatgaaca ggccaaaatc 7921 atccgctgct attcagatgc gccgatcacg cataacggat cagtcatgtt cagcgtggac 7981 aatgagcgaa tgtttcagaa tctcgatttt gcctcctatg acacgtacgc ttcgcaggaa 8041 aacgcctctg cctttttatt gaactgtgat ttatggagaa atctgaaaca agggcgcccg 8101 ttttggattt tggaaacgag tccgtcgtat gccgcctcgc ttgaaagctc cgcttacccg 8161 cacgcagacg ggtatttgca ggccgaagcc gtatcgtcct acgccttagg gagccagggg 8221 ttttgctact ggctatggcg acagcagcgt tcaggcagcg agatttccca cggttcggtt 8281 ctcagtgcct ggggcgaacc caccatcggc tatcaaaatg tgctggcggt tgagcgggca 8341 agaaaggaaa tcgagcctat tattctatcg actgaacccg ttcaagccga ggcggcgatg 8401 acttactctg acagagcaaa agcatttatt aaaactgagc ctcaccgggg actccggcat 8461 cgttcgcttg tgacgcattt ttatgaacgt attctcaaca cggggattca ccgtgacctt 8521 attccggaag gcgctccact ggacggctat cgcttgctgt ttacgccatt tgtgccgtat 8581 ttgtcttctg aatttatcaa aaaagcttcg gcattcgctg aagcgggcgg catctggatc 8641 accgggccgc tgacaggagg acgcacatgc gagcatacca ttcataccga ttgcggactt 8701 ggcgaacttg agaaaacgtc agggatcaaa acacttttta cctttccgat gaatgagaac 8761 gtgaatacag gaaaagcgtt tggcatcacg gcgccgctcg gactgtggag cgcggtgttt 8821 gacacagaga gcggaaacac ccttggcacg gttgaagcag gaccgggggc gggccatgct 8881 tttctgacgg aacggaatta cggcgagggg aaaattgtca tgctgggctc gcttccatcc 8941 gggaaagaag gggatgcgat gctggaagcg ctcgtcaggc attatgcgga ggaagctgtt 9001 atttccagcc ggtcggatgt gacacccggc acgatcgttg ccccgcgtat aggcgaaaac 9061 ggccttgtgt ggatcgttgt gaatatggat ggaaaaggcg ggagcgtgac attgccggaa 9121 tcgggaacgg atttgttgac gcaccgcttg gaaaaggcgg ggagactggc ggtcggaccg 9181 catgaatacc gtgtgattca atttgacaat cacagctaat aatactagag aatcataaaa 9241 aatttatttg ctttgtgagc ggataacaat tataatagat tcaattgtga gcggataaca 9301 atttcacaca tactagagtc acacaggaaa cctactagat gtcctacgac tacgagaaaa 9361 ccagcctcac cctctaccgg gcggtattca aggccaacta cgacggcgac gtcggtcgct 9421 acctgcatcc cgacaaggaa ctcgccgagg ctgcggaagt cgccccgctg ctgcatccga 9481 ccttcgacag ccccaacacc cctggcgtcc ccgcccgcgc gccggacatc gtcgccggcc 9541 gcgacggcct ctacgccccg gacaccggcg gcacctcggt gttcgaccgc gccggcgtgc 9601 tgcgccgcgc cgacggcgac ttcgtgatac ccgacggcac cgacatcccg ccggacctta 9661 aggtgaagca ggacagctac aacaagcgcc tgcaagccac ccactacacc atcatgccgg 9721 ccaagccgat gtaccgggag gtcctcatgg gccaactgga caacttcgtg cgcaacgcca 9781 tccgccgcca atgggaaaaa gcccgcgggc tctagtacta gagccaggca tcaaataaaa 9841 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 9901 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta //