##gff-version 3 ##sequence-region BBa_K1175300 1 9952 BBa_K1175300 . engineered_region 1 4668 . + 0 ID=BBa_K1175028;Name=BBa_K1175028 BBa_K1175300 . promoter 1 130 . + 0 ID=BBa_K206000;Name=BBa_K206000;Parent=BBa_K1175028 BBa_K1175300 . promoter 1 131 . + 0 ID=annotation2049252;Name=promoter;Parent=BBa_K206000 BBa_K1175300 . operator 61 78 . + 0 ID=annotation2049254;Name=AraI2;Parent=BBa_K206000 BBa_K1175300 . operator 40 57 . + 0 ID=annotation2049253;Name=AraI1;Parent=BBa_K206000 BBa_K1175300 . engineered_region 139 4668 . + 0 ID=BBa_K215104;Name=BBa_K215104;Parent=BBa_K1175028 BBa_K1175300 . CDS 3266 4668 . + 0 ID=BBa_K215102;Name=BBa_K215102;Parent=BBa_K215104 BBa_K1175300 . ribosome_entry_site 3268 3279 . + 0 ID=annotation2027449;Name=Native RBS;Parent=BBa_K215102 BBa_K1175300 . CDS 3280 4668 . + 0 ID=annotation2027450;Name=prtF;Parent=BBa_K215102 BBa_K1175300 . CDS 139 1887 . + 0 ID=BBa_K215100;Name=BBa_K215100;Parent=BBa_K215104 BBa_K1175300 . ribosome_entry_site 141 156 . + 0 ID=annotation2027445;Name=native RBS;Parent=BBa_K215100 BBa_K1175300 . CDS 157 1887 . + 0 ID=annotation2027446;Name=prtD;Parent=BBa_K215100 BBa_K1175300 . CDS 1896 3257 . + 0 ID=BBa_K215101;Name=BBa_K215101;Parent=BBa_K215104 BBa_K1175300 . CDS 1911 3257 . + 0 ID=annotation2027448;Name=prtE;Parent=BBa_K215101 BBa_K1175300 . ribosome_entry_site 1898 1910 . + 0 ID=annotation2027447;Name=native rbs;Parent=BBa_K215101 BBa_K1175300 . engineered_region 4677 6192 . + 0 ID=BBa_K875004;Name=BBa_K875004 BBa_K1175300 . tag 6036 6053 . + 0 ID=annotation2194273;Name=tag;Parent=BBa_K875004 BBa_K1175300 . stem_loop 6065 6192 . + 0 ID=annotation2194270;Name=stem_loop;Parent=BBa_K875004 BBa_K1175300 . ribosome_entry_site 4765 4776 . + 0 ID=annotation2194268;Name=rbs;Parent=BBa_K875004 BBa_K1175300 . promoter 4677 4756 . + 0 ID=annotation2194271;Name=promoter;Parent=BBa_K875004 BBa_K1175300 . CDS 4777 6029 . + 0 ID=annotation2194279;Name=protein;Parent=BBa_K875004 BBa_K1175300 . CDS 7228 9222 . + 0 ID=BBa_K1175007;Name=BBa_K1175007 BBa_K1175300 . stop_codon 9216 9222 . + 0 ID=annotation2350875;Name=Double Stop Codon;Parent=BBa_K1175007 BBa_K1175300 . CDS 7231 9215 . + 0 ID=annotation2350874;Name=YesZ Enzyme ;Parent=BBa_K1175007 BBa_K1175300 . start_codon 7228 7230 . + 0 ID=annotation2350873;Name=Start Codon ;Parent=BBa_K1175007 BBa_K1175300 . ribosome_entry_site 6339 6350 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_K1175300 . conserved_region 6343 6346 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K1175300 . CDS 9231 9952 . + 0 ID=BBa_K875008;Name=BBa_K875008 BBa_K1175300 . ribosome_entry_site 9319 9332 . + 0 ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_K875008 BBa_K1175300 . conserved_region 9325 9328 . + 0 ID=annotation23316;Name=conserved;Parent=BBa_B0031 BBa_K1175300 . promoter 9231 9310 . + 0 ID=BBa_K875002;Name=BBa_K875002;Parent=BBa_K875008 BBa_K1175300 . terminator 9824 9952 . + 0 ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K875008 BBa_K1175300 . terminator 9824 9903 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K1175300 . engineered_region 9824 9903 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K1175300 . stem_loop 9835 9878 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K1175300 . terminator 9912 9952 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K1175300 . stop_codon 9945 9945 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K1175300 . polyA_site 9939 9952 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K1175300 . stem_loop 9919 9938 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K1175300 . engineered_region 9912 9952 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K1175300 . CDS 9339 9815 . + 0 ID=BBa_K314200;Name=BBa_K314200;Parent=BBa_K875008 BBa_K1175300 . engineered_region 9339 9815 . + 0 ID=annotation2089456;Name=tse2;Parent=BBa_K314200 BBa_K1175300 . tag 6357 7221 . + 0 ID=BBa_K215001;Name=BBa_K215001 BBa_K1175300 . sequence_feature 6534 6542 . + 0 ID=annotation2027598;Name=GSA linker;Parent=BBa_K215001 BBa_K1175300 . sequence_feature 6441 6460 . + 0 ID=annotation2027591;Name=TEV protease site;Parent=BBa_K215001 BBa_K1175300 . tag 6516 6533 . + 0 ID=annotation2027597;Name=His tag;Parent=BBa_K215001 BBa_K1175300 . sequence_feature 6471 6476 . + 0 ID=annotation2027593;Name=NheI;Parent=BBa_K215001 BBa_K1175300 . sequence_feature 6405 6413 . + 0 ID=annotation2027588;Name=GSA linker;Parent=BBa_K215001 BBa_K1175300 . sequence_feature 6507 6515 . + 0 ID=annotation2027596;Name=GSA linker;Parent=BBa_K215001 BBa_K1175300 . sequence_feature 6477 6485 . + 0 ID=annotation2027594;Name=GSA linker;Parent=BBa_K215001 BBa_K1175300 . tag 6414 6431 . + 0 ID=annotation2027589;Name=His tag;Parent=BBa_K215001 BBa_K1175300 . tag 6543 7091 . + 0 ID=annotation2027599;Name=Secretion tag;Parent=BBa_K215001 BBa_K1175300 . sequence_feature 6486 6506 . + 0 ID=annotation2027595;Name=TEV protease site;Parent=BBa_K215001 BBa_K1175300 . sequence_feature 6432 6440 . + 0 ID=annotation2027590;Name=GSA linker;Parent=BBa_K215001 BBa_K1175300 . sequence_feature 6461 6470 . + 0 ID=annotation2027592;Name=GSA linker;Parent=BBa_K215001 BBa_K1175300 . stop_codon 7092 7221 . + 0 ID=annotation2027600;Name=terminator (B0015);Parent=BBa_K215001 BBa_K1175300 . tag 6357 6404 . + 0 ID=annotation2027587;Name=nanotag;Parent=BBa_K215001 BBa_K1175300 . promoter 6201 6330 . + 0 ID=BBa_K206000;Name=BBa_K206000 BBa_K1175300 . promoter 6201 6331 . + 0 ID=annotation2049252;Name=promoter;Parent=BBa_K206000 BBa_K1175300 . operator 6261 6278 . + 0 ID=annotation2049254;Name=AraI2;Parent=BBa_K206000 BBa_K1175300 . operator 6240 6257 . + 0 ID=annotation2049253;Name=AraI1;Parent=BBa_K206000 >BBa_K1175300 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacg ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaggggagagagccctgccat gaacgcgtctagcgaacgtgaccgttctctgtttggtgtgctgcgtcagttccgccgctccttttggtctgttggcatc ttcagcgcggtcatcaatgttctgatgctggcacctagcgtctacatgctccaggtttacgaccgtgtcctcgccagcg gcaacggtattactctgctgatgctcacgctcctgatggcgggtctgtgcgccttcatgggtgctctcgaatgggttcg cagcctgctggtagttcgtctcggtactcgcatcgacctcgcgctcaatcaagacgtttttaacgcggctttcgcgcgt aatctggaagctggtgatggccgtgccggtctggcgctgaccgacctcaccctgctccgtcaattcatcacgggtaatg cactgttcgcgttcttcgacgttccgtggttcccgctctttctcctcgtactgttcctgctgcacccttggctcggcat gctcgcactgggtggtacggttgtacctggtggcgtaggcctcgcagaaccggcgtctgaccagtctaccgcgggtggc tctaatcaacaaagccaacaagccactcatctcgcggatgcgcagctccgtaatgcggacgttatcgaagcgatgggta tgctcggtaacctccgtcgtcgctggctggcgcgtcactaccgctttatctccctccaaaatctcgcttccgagcgcgc agccgccgttggtggtgcgtccaaatactctcgtattgcactccaaagcctcatgctgggcctcggtgcgctcctcgcg atcgacggcaaaatcacgccgggtatgatgattgcgggctctatcctcgtcggtcgtgttctgtccccgattgaccagc tgatcggtgtttggaaacagtggtcttctgcccgcattgcgtggcagcgcctgacccgtctgattgccgcatatccgcc acgccctgcggctatggctctcccagcgccggaaggccacctgtccgttgagcaagtttctctgcgtaccgcacaaggc aatactcgcctgcaaaacatccatttctccctgcaagcgggtgagaccctggttatcctgggcgcctctggttctggca aatcttctctcgctcgtctgctcgtgggtgcacaatcccctactcaaggtaaagttcgcctcgatggtgcggacctgaa ccaagttgacaaaaacaccttcggtccgaccattggttacctcccgcaggacgttcaactgttcaaaggtagcctggcg gaaaacattgcccgtttcggcgacgccgacccggaaaaggttgttgcggcagctaagctcgccggtgttcacgaactca tcctgtctctgcctaatggttacgacactgagctgggtgacggcggtggcggcctgtctggtggtcaacgtcagcgtat cggtctcgcacgcgcaatgtacggcgacccgtgtctcctcattctggacgaaccgaacgcatctctggactctgagggt gaccaagcactcatgcaggctattgtggcgctccagaaacgtggtgcaaccgttgtactcattacgcaccgtccggcac tcaccaccctcgctcagaaaatcctgatcctccacgagggtcaacagcaacgtatgggcctggcccgtgacgttctgac ggaactccagcagcgctccgcggcaaaccaggcgcgtatgaacccgaccgcagcaatgccacagtaataatactagaga gcagggatctcgtgatgactggtatggatattaccactcaggacgaactcaacgaagctgccatgcgtgaccgtgcgag ccgtgacgaagaacgtgcgctccgtctgggttggtggctcgtcctcgcgggtttcggtggtttcctcctgtgggcgctc ctggctccactggacaagggtgttgcggttcagggtaacgttgtagtttccggtaaccgtaaagttatccagcacatgc aaggcggtattgttgaccgcatccaagttaaggacggcgaccgtgttgccgctggtcaggttctgctcactctcaatgc agtcgacgctcgtaccacctccgagggcctgggttctcagtacgatcagctcatcgcgcgtgaagcgcgcctcctcgcc gagcagcgtaaccagtcttctctcgctgcaacgccacgcctgacgcaggcacgccagcgtccggaaatggcggcgatta tcgccctccaggaagacctgctgcgctctcgtcagcagtctctcaaactggaaattgacggtgtccgtgcatctatcga tggtctggaaacttctctgggtgctctgcaaaaagtgatgtcttctaaacagtctgaacaagcgactctgtctcaacag ctgcaaggtctgcgtccgctggccgcagacaactacgttccgcgtaacaagatgctcgaaaccgaacgtctcttcgccc aggtttctggcgagctggcccagacctctggtgaagttggtcgtacccgtcgtgacattcaacaacagaaactccgcat tgcgcagcgccagcaggagtacgacaaagaagtcaactctgaactgtctgacgttcaggccaaactgaacgaagtaatc tctcagcgtgaaaaggcggacttcaatctcgctaacgtgcaggttcgtgcgccggtggcgggcactgtggtggatatga aaatcttcaccgaaggtggcgtaattgctccgggtcaagttatgatggacattgttccggaggatcagccactgctggt agatggccgtatcccggttgagatggttgacaaagtatggagcggtctgccggtcgaactgcaatttactgcgttttcc caatctactacgccgcgtgtccctggtactgtaaccctgctgagcgcggatcgcctggtggacgaaaaagatggcaccc cttactatggcctccgtattcaggtgtctgaggaaggtaaacgtagcctccacggcctggagattaaaccgggtatgcc tgtccaaggttttgtgcgtacgggcgaacgctctttcatcaactacctcttcaaaccgctcatggaccgtatgcacctc gcactgaccgaagaataatactagagagcggaaggaagcaatgcgccgtaaagcagttctgctcactgtggttctgagc ctgtctggtggtagcgcccaagcgatgggtctgctggacgcgtgggaactcgcgctccgcaatgatgcgcaactgcgtg cggcaggtttcgaacgtgacgcgggtcaagaggaagttgcgattggtcgcgcaggcctcctgccgagcctgcaatacac gtacggtgcgaactactcccactccaaagttacccagcgcgatcgtaccctgaacaacaccacgaaacgtgattatgat aactacgtttctacgctgaccctgcgtcagccactcctggactacgcggcatgggcgcgttatcagcaaggcgtaactc gtaaactgatggcggaccagcgttttcgcgaccgttcccaggatctgatggttcgtctgtaccagtcctggagcgaagc cctgctggcgcaggaaaagctgatgctcctcgacgctcagcgtcgtgcataccaggaacagctcgcactgaatcgccgt ctcctggctgccggtgagggcacgcaaaccgacctgcgtgagaccgaagcacgttataccgtcaccgaggcgcagcgta tcgaacaggaggacaccctcgatgccgccatgactgacctggaaaacatgatgggctctcctctccagatccaagacct gtctccactggctctcgacactctcccggataacgtaacggaaaaccgtagcctctctcagtggcgtgaactcacggta cgtcacaacgccaaactggcagtacaacgtgagaacgtggattactctcgctacgaaatcgagcgcaaccgtgcgggcc acctcccgaccctggacctggttgcctctacccgtaattccctctccgagagcgaatacaactacaatcaaaaatacga cacccagaccgtaggtctgcaagttcgtgttcctctctactctggcggtgcggtttctgcgtctatgcgccaagcggca gctgagtatcaacagtcccaggcggaactggacaatcagactcgtcagaccttcgcggagctgcgtcgccagttcaacc tgtgcgcaaacggtgctgcgaaaatccgtgcttggcagatgtctgtagctgctgccgaagaggccattcgtgcgacccg tcagtctgttgcgggtggtgaacgtattaacctggatgtgctcatggctgagcaggaatggtataatgcccgtcgtgag ctgacggaggttaagtaccgttggctgcaagcgtggctcaatctgcgctacacggctggtactctcaacgaacaagata tgatgcagctggcagcctggttccagtctgcgccggttatcaataaaaccggcatcaatgctgccacgggcaacaagac caattaatactagagaatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaattgtgagcgg ataacaatttcacacatactagagaaagaggagaaatactagaaagaggagaaatactaatgaaagctactaaactggt actgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccg tatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaag ctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcat ggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggt gttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacacca tcggcactcgtccggacaacggcggaggttctggagatattctgctgacccagagcccggcgattctgagcgtgagccc gggcgaacgcgtgagctttagctgccgcgcgagccagagcattggcgtgctgtgcgatcgcattagcattcattggtat cagcagcgcaccaacggcagcccgcgcctgctgattaaatatgcgagcgaaagcattagcggcattccgagccgcttta gcggcagcggcagcggcaccgattttaccctgagcattaacagcgtggaaagcgaagatattgcggattattattgcca gcagagcaacagctggccgtggacctttggcggcggcaccaaactggaaattaaaggcggcaaaggcagcggcggcaaa ggcaccggcggcaaaggcagcggcggcaaaggcagcgaagtgaaactggtggaaagcggcggcgatctggtgcagccgg gcggcagcctgaaactgagctgcgcggtgcattgcgatcgcaccagcggctttacctttagcgattattatatgtattg ggtgcgccagaccccggaaaaacgcctggaatgggtggcgtatattagcagcgtgcattgcgatcgcggcggcggcagc accttttatccagataccgtgaaaggccgctttaccattagccgcgataacgcgaaaaacaccctgtatctacagatga gccgcctgaaaagcgatgataccgcgatgtattattgcgcgcgcgcgtatcatggcaactattttgattattggggcca gggcaccaccctgaccgtgagcagctccggtcaccaccaccaccaccactgatactagagccaggcatcaaataaaacg aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgct caccttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacactttgctatgccata gcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgttttt ttgggctagctactagagaaagaggagaaatactagatggacgttgaagcatggctgggtgcccgtgtaccgctggttg aaacgggcagcgcacatcaccaccatcatcatggttccgccgaaaacctctactttcagggcggctctgctgctagcgg ttctgccgagaatctgtacttccaaggtggctccgctcatcaccaccatcatcacggtagcgcaggcggcactgacacc tttgacttctctggttactctaacaatcagcgtatcaacctgaacgagggttctttctctgacgttggtggtctgaagg gcaacgtttctatcgcgcatggtgttaccatcgaaaacgcgatcggtggttctggtaacgacatcctgatcggtaatgg cgcggataacatcctccagggtggtgcgggtgacgacgttctgtacggttctacgggtgcggacacgctgacgggtggc gctggtcgtgacattttcgtatatggtagcggtcaagactctaccgtttctgcatacgactggatcacggattttcaga cgggtatcgacaaaatcgacctgtccgctttccgtaatgaaggtcagctgtcttttgttcaggaccagtttactggtaa aggccaggaggttatgctgcaatgggacgcggcgaactctacgaccaatctgtggctgcacgaagcgggtcactcttct gttgactttctcgttcgtatcgttggccagaccgcgcagagcgacattatcgtctaataaccaggcatcaaataaaacg aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc tcaccttcgggtgggcctttctgcgtttatattactagatgagaaaactgtatcatggcgcttgctattatccggaatt atgggatgaagagacgattcagcaggacattgacatcatgcgtgaagttggcgtaaatgttgtgcggatcggcgaattt gcctggtcagtcatggaacctgaagaaggaaaaattgacgtcggttttttcaaagaaatcatcgcccggctgtatgata gcgggatcgaaacgattatgtgcacgccgacgcctaccccgccgatttggttctcacatggccggcccgaacgcatgca tgccaatgaaaaaagagagatcatggggcatggctcccgtcagcatgcctgtacgaacaacccgtatttccgaaaaaaa gccgccatcatcaccacagccatcgccaaggagcttggccggctcccggggctgatcggatggcagctagacaatgagt ttaaatgccatgttgcagaatgcatgtgtgagacatgcttgcgcctatggcatgactggctcaaaaatcgctacggggt aattgagcgcttgaatgaagcttggggaaccgatgtgtggagcgagacctatcagacgtttgagcaagtcccgcagccg ggaccggccccgtttctgcatcatgcctctctacgcactatgtatcagctgttttcgatggagatgatcgcttcgtttg cggatgaacaggccaaaatcatccgctgctattcagatgcgccgatcacgcataacggatcagtcatgttcagcgtgga caatgagcgaatgtttcagaatctcgattttgcctcctatgacacgtacgcttcgcaggaaaacgcctctgccttttta ttgaactgtgatttatggagaaatctgaaacaagggcgcccgttttggattttggaaacgagtccgtcgtatgccgcct cgcttgaaagctccgcttacccgcacgcagacgggtatttgcaggccgaagccgtatcgtcctacgccttagggagcca ggggttttgctactggctatggcgacagcagcgttcaggcagcgagatttcccacggttcggttctcagtgcctggggc gaacccaccatcggctatcaaaatgtgctggcggttgagcgggcaagaaaggaaatcgagcctattattctatcgactg aacccgttcaagccgaggcggcgatgacttactctgacagagcaaaagcatttattaaaactgagcctcaccggggact ccggcatcgttcgcttgtgacgcatttttatgaacgtattctcaacacggggattcaccgtgaccttattccggaaggc gctccactggacggctatcgcttgctgtttacgccatttgtgccgtatttgtcttctgaatttatcaaaaaagcttcgg cattcgctgaagcgggcggcatctggatcaccgggccgctgacaggaggacgcacatgcgagcataccattcataccga ttgcggacttggcgaacttgagaaaacgtcagggatcaaaacactttttacctttccgatgaatgagaacgtgaataca ggaaaagcgtttggcatcacggcgccgctcggactgtggagcgcggtgtttgacacagagagcggaaacacccttggca cggttgaagcaggaccgggggcgggccatgcttttctgacggaacggaattacggcgaggggaaaattgtcatgctggg ctcgcttccatccgggaaagaaggggatgcgatgctggaagcgctcgtcaggcattatgcggaggaagctgttatttcc agccggtcggatgtgacacccggcacgatcgttgccccgcgtataggcgaaaacggccttgtgtggatcgttgtgaata tggatggaaaaggcgggagcgtgacattgccggaatcgggaacggatttgttgacgcaccgcttggaaaaggcggggag actggcggtcggaccgcatgaataccgtgtgattcaatttgacaatcacagctaataatactagagaatcataaaaaat ttatttgctttgtgagcggataacaattataatagattcaattgtgagcggataacaatttcacacatactagagtcac acaggaaacctactagatgtcctacgactacgagaaaaccagcctcaccctctaccgggcggtattcaaggccaactac gacggcgacgtcggtcgctacctgcatcccgacaaggaactcgccgaggctgcggaagtcgccccgctgctgcatccga ccttcgacagccccaacacccctggcgtccccgcccgcgcgccggacatcgtcgccggccgcgacggcctctacgcccc ggacaccggcggcacctcggtgttcgaccgcgccggcgtgctgcgccgcgccgacggcgacttcgtgatacccgacggc accgacatcccgccggaccttaaggtgaagcaggacagctacaacaagcgcctgcaagccacccactacaccatcatgc cggccaagccgatgtaccgggaggtcctcatgggccaactggacaacttcgtgcgcaacgccatccgccgccaatggga aaaagcccgcgggctctagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata