BBa_K215104 1 BBa_K215104 prtD_prtE_prtF from erwinia chrysanthemi 2009-09-29T11:00:00Z 2015-05-08T01:11:30Z Gene Bank ID: Erwinia chrysanthemi M60395.1 This is the 3 genes: prtD, prtE, prtF, that are from a type I Erwinia chrysanthemi secretion system. false false _320_ 0 5596 9 It's complicated false Synthesized to be bio-bricked, keeps original protein sequence and ribosome binding site. false Josef Dunbar component2027471 1 BBa_K215101 component2027468 1 BBa_K215100 component2027474 1 BBa_K215102 annotation2027474 1 BBa_K215102 range2027474 1 3128 4530 annotation2027468 1 BBa_K215100 range2027468 1 1 1749 annotation2027471 1 BBa_K215101 range2027471 1 1758 3119 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K206000 1 pBAD pBAD strong 2009-10-13T11:00:00Z 2015-05-08T01:11:23Z The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1]. Released HQ 2013 pBAD is an <i>E.coli</i> promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter. false false _307_ 0 4172 9 In stock true There were no special design considerations. false Amelia Hardjasa annotation2049252 1 promoter range2049252 1 1 131 annotation2049254 1 AraI2 range2049254 1 61 78 annotation2049253 1 AraI1 range2049253 1 40 57 BBa_K215001 1 BBa_K215001 Secretion Signal and Streptavidin Binding Tags 2009-09-29T11:00:00Z 2015-05-08T01:11:29Z Nanotag (bases 1-48): From Lamla and Erdmann (http://www.ncbi.nlm.nih.gov/pubmed/14680960). Secretion tag (last 181 AA): Gene Bank ID Erwinia chrysanthemi M60395.1 This part is the vector into which afp is inserted to tag it for purification using the IPP system. It contains an NheI restriction site that allows for the insertion of the desired protein coding sequence (NheI is compatible with XbaI and SpeI). This Target Vector part contains two tags: a secretion tag that causes the protein to be exported to the extracellular space, and a nanotag that causes the protein to bind to stretavidin (or some analog). For this part to function properly, it must be combined with a promoter and RBS, and used in E.coli strain UW5alpha, which has two other plasmids containing the other genes required for proper secretion and display of the protein to be purified on the outer membrane. false false _320_ 0 2811 9 It's complicated false NheI was chosen as the protein insertion site because it is compatible with digested XbaI and SpeI restriction sites. The TEV sites and HIS tags flanking the NheI site are present to separate the inserted protein from the added tags. GSA linkers were used to separate the various features of the part. false Jeff Nivala annotation2027599 1 Secretion tag range2027599 1 187 735 annotation2027591 1 TEV protease site range2027591 1 85 104 annotation2027597 1 His tag range2027597 1 160 177 annotation2027587 1 nanotag range2027587 1 1 48 annotation2027588 1 GSA linker range2027588 1 49 57 annotation2027596 1 GSA linker range2027596 1 151 159 annotation2027589 1 His tag range2027589 1 58 75 annotation2027594 1 GSA linker range2027594 1 121 129 annotation2027598 1 GSA linker range2027598 1 178 186 annotation2027595 1 TEV protease site range2027595 1 130 150 annotation2027590 1 GSA linker range2027590 1 76 84 annotation2027592 1 GSA linker range2027592 1 105 114 annotation2027600 1 terminator (B0015) range2027600 1 736 865 annotation2027593 1 NheI range2027593 1 115 120 BBa_K1175007 1 BBa_K1175007 beta-galacturonidase (YesZ) from Bacillus Subtilis 168 2013-09-16T11:00:00Z 2015-05-08T01:09:35Z Bacillus Subtilis 168 genomic DNA yesZ The enzyme acts on the terminal end of side chains of Rhamnogalacturonan I Pectin, releasing the free galactose. This gene has been isolated from the bacterium Bacillus subtilis subtilis 168. The enzyme yesZ has beta-galactosidase (beta-galacturonidase) activity, cleaving (1&#8594;4)-&#946;-D-galactans to produce single galactose molecules from RG I Pectin. Usage and Biology Pectin is a component of the primary cell wall of plants. As galactose can be metabolized by the human body and by animals, it is an alternative to the usual target of nutrition/energy boosts in biotechnology, glucose. (1) http://www.ncbi.nlm.nih.gov/pubmed/17449691 (2) http://subtiwiki.uni-goettingen.de/wiki/index.php/YesZ (3) http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1694227/ (4) http://aem.asm.org/content/73/12/3803 (5) http://www.tandfonline.com/doi/full/10.1080/15583724.2011.615962#tabModule false false _1488_ 0 14098 9 It's complicated false No design considerations were needed, although we did add a second stop codon. false Nicholas K. Goldner annotation2350875 1 Double Stop Codon range2350875 1 1989 1995 annotation2350874 1 YesZ Enzyme range2350874 1 4 1988 annotation2350873 1 Start Codon range2350873 1 1 3 BBa_K215101 1 BBa_K215101 prtE from erwinia chrysanthemi 2009-09-29T11:00:00Z 2015-05-08T01:11:30Z Gene Bank ID: Erwinia chrysanthemi M60395.1 This is one of the 3 genes: prtD, prtE, prtF, that are from a type I Erwinia chrysanthemi secretion system. false false _320_ 0 5596 9 It's complicated false Synthesized to be bio-bricked, keeps original protein sequence and rhibosome binding site. false Josef Dunbar annotation2027448 1 prtE range2027448 1 16 1362 annotation2027447 1 native rbs range2027447 1 3 15 BBa_K1175300 1 BBa_K1175300 the whole magilua 2013-09-16T11:00:00Z 2015-05-08T01:09:35Z errywhere blah de blah false false _1488_ 0 14098 9 Not in stock false nada bro false Nicholas K. Goldner component2351985 1 BBa_K206000 component2352019 1 BBa_K875008 component2352002 1 BBa_K215001 component2351981 1 BBa_K875004 component2352006 1 BBa_K1175007 component2351987 1 BBa_B0034 component2351975 1 BBa_K1175028 annotation2351975 1 BBa_K1175028 range2351975 1 1 4668 annotation2351981 1 BBa_K875004 range2351981 1 4677 6192 annotation2352006 1 BBa_K1175007 range2352006 1 7228 9222 annotation2351987 1 BBa_B0034 range2351987 1 6339 6350 annotation2352019 1 BBa_K875008 range2352019 1 9231 9952 annotation2352002 1 BBa_K215001 range2352002 1 6357 7221 annotation2351985 1 BBa_K206000 range2351985 1 6201 6330 BBa_K1175028 1 BBa_K1175028 Strong pBAD promoter BBa_K206000 attached to SecI pump BBa_K215104 2013-09-16T11:00:00Z 2015-05-08T01:09:35Z iGEM registry, acquired from University of Washington 2009 iGEM team from the genomic DNA of Erwinia chrysanthemi These are the three essential parts prtD, prtE, prtF, that make up the Type I secretion system from Erwinia chrysanthemi.This part is made of 3 genes: prtD, prtE, and prtF, that constitute a type I Erwinia chrysanthemi secretion system. The Pump is expressed from a strong constitutive promoter, BBa_k206000, and has the translational terminator BBa_B0014. In pSB1C3. Used to secrete proteins containing prtB C-terminal tag. The prtB C-terminal tag is built into the protein generator [BBa_K215002]. Any protein of interest can be inserted into the protein generator and then secreted when used in conjunction with this secretion system. For full characterization data on this part, please see the University of Washington 2009 iGEM project page and Wisconsin Lutheran College 2013 iGEM project page. false false _1488_ 0 14098 9 It's complicated false We had to remove the RBS and in the future will be removing all Bative RBSs from the sequence to be replaced with BBa_B0034 false Nicholas K. Goldner component2350962 1 BBa_K206000 component2350972 1 BBa_K215104 annotation2350962 1 BBa_K206000 range2350962 1 1 130 annotation2350972 1 BBa_K215104 range2350972 1 139 4668 BBa_K875004 1 BBa_K875004 T5LacO-LPP-OmpA-scFv (scFv 54.6 antinorovirus)-6HIS-TT 2012-09-22T11:00:00Z 2015-05-08T01:13:38Z DNA synthesis. 1.???Transport and anchoring of 8-lactamase to the external surface of Escherichia coli??? J. A. Francisco, C. F. Earhart and G. Georgiou. Proc. Natl. Acad. Sci. USA Vol. 89, pp. 2713-2717, April 1992 Biochemistry. 2. ???Recombinant norovirus-specific scFv inhibit virus-like particle binding to cellular ligands??? K. Ettayebi and M. E. Hardy. Published: 31 January 2008 in Virology Journal 2008, 5:21? This construct is designed for the expression of an already descibed engeneered antinorovirus (NoV) monoclonal antibody (mAb 54.6) in fusion with LPP-OmpA. The antibody is expressed in a single chain fragment variable (scFv) format containing light (VL) and heavy (VH) variable domains separeted by a flexible peptide linker. It has already been reported that the scFv 54.6 binds a native recombinant NoV particles (VLPs) and inhibits VLP interaction with cells. LPP-OmpA functions as a leader sequence and an anchor to display the scFv ot the bacterial surface.The construct consistes of T5 Lac Operator (Bba_K875002), ribosomal binding site, LPP-OmpA-scFv 54.6 antinorovirus, Histidine tag (6HIS), Terminator (B0015). false false _1136_ 0 12664 9 It's complicated true This coding sequence was optimized for the expression in prokaryotic cells. It has a Histidine tag. false Marija Drikic, Giorgio Gargari annotation2194273 1 tag range2194273 1 1360 1377 annotation2194270 1 stem_loop range2194270 1 1389 1516 annotation2194268 1 rbs range2194268 1 89 100 annotation2194271 1 promoter range2194271 1 1 80 annotation2194279 1 protein range2194279 1 101 1353 BBa_K314200 1 Tse2 Toxin Tse2 2010-10-17T11:00:00Z 2015-05-08T01:11:55Z pseudomonas aeruginosa A toxic protein originating from pseudomonas aeruginosa that has been shown to arrest growth in both prokaryotic and eukaryotic cells when expressed intracelluarly. It is a substrate p. aruginosas type 6 secretion system false false _496_ 0 6395 9 It's complicated true mention something about promoter needs to have no leaky expression for succesful cloning false Matthew Coyne, Ingrid Swanson, Jesa Landis, Matthew Harger annotation2089456 1 tse2 range2089456 1 1 477 BBa_B0031 1 BBa_B0031 RBS.2 (weak) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false <P> <P>Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-1&quot; in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Cho</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23316 1 conserved range23316 1 7 10 BBa_K215102 1 BBa_K215102 prtF from erwinia chrysanthemi 2009-09-29T11:00:00Z 2015-05-08T01:11:30Z Gene Bank ID: Erwinia chrysanthemi M60395.1 This is one of the 3 genes: prtD, prtE, prtF, that are from a type I Erwinia chrysanthemi secretion system. false false _320_ 0 5596 9 It's complicated false Synthesized to be bio-bricked, keeps original protein sequence and ribosome binding site. false Josef Dunbar annotation2027449 1 Native RBS range2027449 1 3 14 annotation2027450 1 prtF range2027450 1 15 1403 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K875008 1 BBa_K875008 IPTG inducible Tse2 toxin 2012-09-22T11:00:00Z 2015-05-08T01:13:38Z Part Registry The IPTG inducible T5 lac operator (BBa_K875002) regulates the expression of the Tse2 toxin (BBa_K314200). false false _1136_ 0 12657 9 It's complicated false Standard assembly false Giulia Corso, Sara Samari component2194253 1 BBa_K875002 component2194255 1 BBa_B0031 component2194264 1 BBa_B0015 component2194257 1 BBa_K314200 annotation2194255 1 BBa_B0031 range2194255 1 89 102 annotation2194253 1 BBa_K875002 range2194253 1 1 80 annotation2194264 1 BBa_B0015 range2194264 1 594 722 annotation2194257 1 BBa_K314200 range2194257 1 109 585 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K875002 1 BBa_K875002 T5 Lac operator 2012-09-22T11:00:00Z 2015-05-08T01:13:38Z . This promoter is composed by phage T5 promoter and Lac operator. It is repressed in the presence of the lac repressor protein. Trieste Team 2012 used this inducible promotor in the first phase of their project to test every construct that could have been toxic for the host strain, if expressed in a constitutive way. false false _1136_ 0 12660 9 It's complicated false This basic part has been designed in order to have a prefix and a suffix according to the standard Biobrick structure. false Federico Colombo, Elisa Clagnan BBa_K215100 1 BBa_K215100 prtD from erwinia chrysanthemi 2009-09-29T11:00:00Z 2015-05-08T01:11:30Z Gene Bank ID: Erwinia chrysanthemi M60395.1 This is one of the 3 genes: prtD, prtE, prtF, that are from a type I Erwinia chrysanthemi secretion system. false false _320_ 0 5596 9 It's complicated false Synthesized to be bio-bricked, keeps original protein sequence. false Josef Dunbar annotation2027445 1 native RBS range2027445 1 3 18 annotation2027446 1 prtD range2027446 1 19 1749 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_K215102_sequence 1 agcggaaggaagcaatgcgccgtaaagcagttctgctcactgtggttctgagcctgtctggtggtagcgcccaagcgatgggtctgctggacgcgtgggaactcgcgctccgcaatgatgcgcaactgcgtgcggcaggtttcgaacgtgacgcgggtcaagaggaagttgcgattggtcgcgcaggcctcctgccgagcctgcaatacacgtacggtgcgaactactcccactccaaagttacccagcgcgatcgtaccctgaacaacaccacgaaacgtgattatgataactacgtttctacgctgaccctgcgtcagccactcctggactacgcggcatgggcgcgttatcagcaaggcgtaactcgtaaactgatggcggaccagcgttttcgcgaccgttcccaggatctgatggttcgtctgtaccagtcctggagcgaagccctgctggcgcaggaaaagctgatgctcctcgacgctcagcgtcgtgcataccaggaacagctcgcactgaatcgccgtctcctggctgccggtgagggcacgcaaaccgacctgcgtgagaccgaagcacgttataccgtcaccgaggcgcagcgtatcgaacaggaggacaccctcgatgccgccatgactgacctggaaaacatgatgggctctcctctccagatccaagacctgtctccactggctctcgacactctcccggataacgtaacggaaaaccgtagcctctctcagtggcgtgaactcacggtacgtcacaacgccaaactggcagtacaacgtgagaacgtggattactctcgctacgaaatcgagcgcaaccgtgcgggccacctcccgaccctggacctggttgcctctacccgtaattccctctccgagagcgaatacaactacaatcaaaaatacgacacccagaccgtaggtctgcaagttcgtgttcctctctactctggcggtgcggtttctgcgtctatgcgccaagcggcagctgagtatcaacagtcccaggcggaactggacaatcagactcgtcagaccttcgcggagctgcgtcgccagttcaacctgtgcgcaaacggtgctgcgaaaatccgtgcttggcagatgtctgtagctgctgccgaagaggccattcgtgcgacccgtcagtctgttgcgggtggtgaacgtattaacctggatgtgctcatggctgagcaggaatggtataatgcccgtcgtgagctgacggaggttaagtaccgttggctgcaagcgtggctcaatctgcgctacacggctggtactctcaacgaacaagatatgatgcagctggcagcctggttccagtctgcgccggttatcaataaaaccggcatcaatgctgccacgggcaacaagaccaattaa BBa_B0034_sequence 1 aaagaggagaaa BBa_K1175300_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaggggagagagccctgccatgaacgcgtctagcgaacgtgaccgttctctgtttggtgtgctgcgtcagttccgccgctccttttggtctgttggcatcttcagcgcggtcatcaatgttctgatgctggcacctagcgtctacatgctccaggtttacgaccgtgtcctcgccagcggcaacggtattactctgctgatgctcacgctcctgatggcgggtctgtgcgccttcatgggtgctctcgaatgggttcgcagcctgctggtagttcgtctcggtactcgcatcgacctcgcgctcaatcaagacgtttttaacgcggctttcgcgcgtaatctggaagctggtgatggccgtgccggtctggcgctgaccgacctcaccctgctccgtcaattcatcacgggtaatgcactgttcgcgttcttcgacgttccgtggttcccgctctttctcctcgtactgttcctgctgcacccttggctcggcatgctcgcactgggtggtacggttgtacctggtggcgtaggcctcgcagaaccggcgtctgaccagtctaccgcgggtggctctaatcaacaaagccaacaagccactcatctcgcggatgcgcagctccgtaatgcggacgttatcgaagcgatgggtatgctcggtaacctccgtcgtcgctggctggcgcgtcactaccgctttatctccctccaaaatctcgcttccgagcgcgcagccgccgttggtggtgcgtccaaatactctcgtattgcactccaaagcctcatgctgggcctcggtgcgctcctcgcgatcgacggcaaaatcacgccgggtatgatgattgcgggctctatcctcgtcggtcgtgttctgtccccgattgaccagctgatcggtgtttggaaacagtggtcttctgcccgcattgcgtggcagcgcctgacccgtctgattgccgcatatccgccacgccctgcggctatggctctcccagcgccggaaggccacctgtccgttgagcaagtttctctgcgtaccgcacaaggcaatactcgcctgcaaaacatccatttctccctgcaagcgggtgagaccctggttatcctgggcgcctctggttctggcaaatcttctctcgctcgtctgctcgtgggtgcacaatcccctactcaaggtaaagttcgcctcgatggtgcggacctgaaccaagttgacaaaaacaccttcggtccgaccattggttacctcccgcaggacgttcaactgttcaaaggtagcctggcggaaaacattgcccgtttcggcgacgccgacccggaaaaggttgttgcggcagctaagctcgccggtgttcacgaactcatcctgtctctgcctaatggttacgacactgagctgggtgacggcggtggcggcctgtctggtggtcaacgtcagcgtatcggtctcgcacgcgcaatgtacggcgacccgtgtctcctcattctggacgaaccgaacgcatctctggactctgagggtgaccaagcactcatgcaggctattgtggcgctccagaaacgtggtgcaaccgttgtactcattacgcaccgtccggcactcaccaccctcgctcagaaaatcctgatcctccacgagggtcaacagcaacgtatgggcctggcccgtgacgttctgacggaactccagcagcgctccgcggcaaaccaggcgcgtatgaacccgaccgcagcaatgccacagtaataatactagagagcagggatctcgtgatgactggtatggatattaccactcaggacgaactcaacgaagctgccatgcgtgaccgtgcgagccgtgacgaagaacgtgcgctccgtctgggttggtggctcgtcctcgcgggtttcggtggtttcctcctgtgggcgctcctggctccactggacaagggtgttgcggttcagggtaacgttgtagtttccggtaaccgtaaagttatccagcacatgcaaggcggtattgttgaccgcatccaagttaaggacggcgaccgtgttgccgctggtcaggttctgctcactctcaatgcagtcgacgctcgtaccacctccgagggcctgggttctcagtacgatcagctcatcgcgcgtgaagcgcgcctcctcgccgagcagcgtaaccagtcttctctcgctgcaacgccacgcctgacgcaggcacgccagcgtccggaaatggcggcgattatcgccctccaggaagacctgctgcgctctcgtcagcagtctctcaaactggaaattgacggtgtccgtgcatctatcgatggtctggaaacttctctgggtgctctgcaaaaagtgatgtcttctaaacagtctgaacaagcgactctgtctcaacagctgcaaggtctgcgtccgctggccgcagacaactacgttccgcgtaacaagatgctcgaaaccgaacgtctcttcgcccaggtttctggcgagctggcccagacctctggtgaagttggtcgtacccgtcgtgacattcaacaacagaaactccgcattgcgcagcgccagcaggagtacgacaaagaagtcaactctgaactgtctgacgttcaggccaaactgaacgaagtaatctctcagcgtgaaaaggcggacttcaatctcgctaacgtgcaggttcgtgcgccggtggcgggcactgtggtggatatgaaaatcttcaccgaaggtggcgtaattgctccgggtcaagttatgatggacattgttccggaggatcagccactgctggtagatggccgtatcccggttgagatggttgacaaagtatggagcggtctgccggtcgaactgcaatttactgcgttttcccaatctactacgccgcgtgtccctggtactgtaaccctgctgagcgcggatcgcctggtggacgaaaaagatggcaccccttactatggcctccgtattcaggtgtctgaggaaggtaaacgtagcctccacggcctggagattaaaccgggtatgcctgtccaaggttttgtgcgtacgggcgaacgctctttcatcaactacctcttcaaaccgctcatggaccgtatgcacctcgcactgaccgaagaataatactagagagcggaaggaagcaatgcgccgtaaagcagttctgctcactgtggttctgagcctgtctggtggtagcgcccaagcgatgggtctgctggacgcgtgggaactcgcgctccgcaatgatgcgcaactgcgtgcggcaggtttcgaacgtgacgcgggtcaagaggaagttgcgattggtcgcgcaggcctcctgccgagcctgcaatacacgtacggtgcgaactactcccactccaaagttacccagcgcgatcgtaccctgaacaacaccacgaaacgtgattatgataactacgtttctacgctgaccctgcgtcagccactcctggactacgcggcatgggcgcgttatcagcaaggcgtaactcgtaaactgatggcggaccagcgttttcgcgaccgttcccaggatctgatggttcgtctgtaccagtcctggagcgaagccctgctggcgcaggaaaagctgatgctcctcgacgctcagcgtcgtgcataccaggaacagctcgcactgaatcgccgtctcctggctgccggtgagggcacgcaaaccgacctgcgtgagaccgaagcacgttataccgtcaccgaggcgcagcgtatcgaacaggaggacaccctcgatgccgccatgactgacctggaaaacatgatgggctctcctctccagatccaagacctgtctccactggctctcgacactctcccggataacgtaacggaaaaccgtagcctctctcagtggcgtgaactcacggtacgtcacaacgccaaactggcagtacaacgtgagaacgtggattactctcgctacgaaatcgagcgcaaccgtgcgggccacctcccgaccctggacctggttgcctctacccgtaattccctctccgagagcgaatacaactacaatcaaaaatacgacacccagaccgtaggtctgcaagttcgtgttcctctctactctggcggtgcggtttctgcgtctatgcgccaagcggcagctgagtatcaacagtcccaggcggaactggacaatcagactcgtcagaccttcgcggagctgcgtcgccagttcaacctgtgcgcaaacggtgctgcgaaaatccgtgcttggcagatgtctgtagctgctgccgaagaggccattcgtgcgacccgtcagtctgttgcgggtggtgaacgtattaacctggatgtgctcatggctgagcaggaatggtataatgcccgtcgtgagctgacggaggttaagtaccgttggctgcaagcgtggctcaatctgcgctacacggctggtactctcaacgaacaagatatgatgcagctggcagcctggttccagtctgcgccggttatcaataaaaccggcatcaatgctgccacgggcaacaagaccaattaatactagagaatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagaaagaggagaaatactaatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggagatattctgctgacccagagcccggcgattctgagcgtgagcccgggcgaacgcgtgagctttagctgccgcgcgagccagagcattggcgtgctgtgcgatcgcattagcattcattggtatcagcagcgcaccaacggcagcccgcgcctgctgattaaatatgcgagcgaaagcattagcggcattccgagccgctttagcggcagcggcagcggcaccgattttaccctgagcattaacagcgtggaaagcgaagatattgcggattattattgccagcagagcaacagctggccgtggacctttggcggcggcaccaaactggaaattaaaggcggcaaaggcagcggcggcaaaggcaccggcggcaaaggcagcggcggcaaaggcagcgaagtgaaactggtggaaagcggcggcgatctggtgcagccgggcggcagcctgaaactgagctgcgcggtgcattgcgatcgcaccagcggctttacctttagcgattattatatgtattgggtgcgccagaccccggaaaaacgcctggaatgggtggcgtatattagcagcgtgcattgcgatcgcggcggcggcagcaccttttatccagataccgtgaaaggccgctttaccattagccgcgataacgcgaaaaacaccctgtatctacagatgagccgcctgaaaagcgatgataccgcgatgtattattgcgcgcgcgcgtatcatggcaactattttgattattggggccagggcaccaccctgaccgtgagcagctccggtcaccaccaccaccaccactgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgctcaccttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatggacgttgaagcatggctgggtgcccgtgtaccgctggttgaaacgggcagcgcacatcaccaccatcatcatggttccgccgaaaacctctactttcagggcggctctgctgctagcggttctgccgagaatctgtacttccaaggtggctccgctcatcaccaccatcatcacggtagcgcaggcggcactgacacctttgacttctctggttactctaacaatcagcgtatcaacctgaacgagggttctttctctgacgttggtggtctgaagggcaacgtttctatcgcgcatggtgttaccatcgaaaacgcgatcggtggttctggtaacgacatcctgatcggtaatggcgcggataacatcctccagggtggtgcgggtgacgacgttctgtacggttctacgggtgcggacacgctgacgggtggcgctggtcgtgacattttcgtatatggtagcggtcaagactctaccgtttctgcatacgactggatcacggattttcagacgggtatcgacaaaatcgacctgtccgctttccgtaatgaaggtcagctgtcttttgttcaggaccagtttactggtaaaggccaggaggttatgctgcaatgggacgcggcgaactctacgaccaatctgtggctgcacgaagcgggtcactcttctgttgactttctcgttcgtatcgttggccagaccgcgcagagcgacattatcgtctaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatattactagatgagaaaactgtatcatggcgcttgctattatccggaattatgggatgaagagacgattcagcaggacattgacatcatgcgtgaagttggcgtaaatgttgtgcggatcggcgaatttgcctggtcagtcatggaacctgaagaaggaaaaattgacgtcggttttttcaaagaaatcatcgcccggctgtatgatagcgggatcgaaacgattatgtgcacgccgacgcctaccccgccgatttggttctcacatggccggcccgaacgcatgcatgccaatgaaaaaagagagatcatggggcatggctcccgtcagcatgcctgtacgaacaacccgtatttccgaaaaaaagccgccatcatcaccacagccatcgccaaggagcttggccggctcccggggctgatcggatggcagctagacaatgagtttaaatgccatgttgcagaatgcatgtgtgagacatgcttgcgcctatggcatgactggctcaaaaatcgctacggggtaattgagcgcttgaatgaagcttggggaaccgatgtgtggagcgagacctatcagacgtttgagcaagtcccgcagccgggaccggccccgtttctgcatcatgcctctctacgcactatgtatcagctgttttcgatggagatgatcgcttcgtttgcggatgaacaggccaaaatcatccgctgctattcagatgcgccgatcacgcataacggatcagtcatgttcagcgtggacaatgagcgaatgtttcagaatctcgattttgcctcctatgacacgtacgcttcgcaggaaaacgcctctgcctttttattgaactgtgatttatggagaaatctgaaacaagggcgcccgttttggattttggaaacgagtccgtcgtatgccgcctcgcttgaaagctccgcttacccgcacgcagacgggtatttgcaggccgaagccgtatcgtcctacgccttagggagccaggggttttgctactggctatggcgacagcagcgttcaggcagcgagatttcccacggttcggttctcagtgcctggggcgaacccaccatcggctatcaaaatgtgctggcggttgagcgggcaagaaaggaaatcgagcctattattctatcgactgaacccgttcaagccgaggcggcgatgacttactctgacagagcaaaagcatttattaaaactgagcctcaccggggactccggcatcgttcgcttgtgacgcatttttatgaacgtattctcaacacggggattcaccgtgaccttattccggaaggcgctccactggacggctatcgcttgctgtttacgccatttgtgccgtatttgtcttctgaatttatcaaaaaagcttcggcattcgctgaagcgggcggcatctggatcaccgggccgctgacaggaggacgcacatgcgagcataccattcataccgattgcggacttggcgaacttgagaaaacgtcagggatcaaaacactttttacctttccgatgaatgagaacgtgaatacaggaaaagcgtttggcatcacggcgccgctcggactgtggagcgcggtgtttgacacagagagcggaaacacccttggcacggttgaagcaggaccgggggcgggccatgcttttctgacggaacggaattacggcgaggggaaaattgtcatgctgggctcgcttccatccgggaaagaaggggatgcgatgctggaagcgctcgtcaggcattatgcggaggaagctgttatttccagccggtcggatgtgacacccggcacgatcgttgccccgcgtataggcgaaaacggccttgtgtggatcgttgtgaatatggatggaaaaggcgggagcgtgacattgccggaatcgggaacggatttgttgacgcaccgcttggaaaaggcggggagactggcggtcggaccgcatgaataccgtgtgattcaatttgacaatcacagctaataatactagagaatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaattgtgagcggataacaatttcacacatactagagtcacacaggaaacctactagatgtcctacgactacgagaaaaccagcctcaccctctaccgggcggtattcaaggccaactacgacggcgacgtcggtcgctacctgcatcccgacaaggaactcgccgaggctgcggaagtcgccccgctgctgcatccgaccttcgacagccccaacacccctggcgtccccgcccgcgcgccggacatcgtcgccggccgcgacggcctctacgccccggacaccggcggcacctcggtgttcgaccgcgccggcgtgctgcgccgcgccgacggcgacttcgtgatacccgacggcaccgacatcccgccggaccttaaggtgaagcaggacagctacaacaagcgcctgcaagccacccactacaccatcatgccggccaagccgatgtaccgggaggtcctcatgggccaactggacaacttcgtgcgcaacgccatccgccgccaatgggaaaaagcccgcgggctctagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K215001_sequence 1 atggacgttgaagcatggctgggtgcccgtgtaccgctggttgaaacgggcagcgcacatcaccaccatcatcatggttccgccgaaaacctctactttcagggcggctctgctgctagcggttctgccgagaatctgtacttccaaggtggctccgctcatcaccaccatcatcacggtagcgcaggcggcactgacacctttgacttctctggttactctaacaatcagcgtatcaacctgaacgagggttctttctctgacgttggtggtctgaagggcaacgtttctatcgcgcatggtgttaccatcgaaaacgcgatcggtggttctggtaacgacatcctgatcggtaatggcgcggataacatcctccagggtggtgcgggtgacgacgttctgtacggttctacgggtgcggacacgctgacgggtggcgctggtcgtgacattttcgtatatggtagcggtcaagactctaccgtttctgcatacgactggatcacggattttcagacgggtatcgacaaaatcgacctgtccgctttccgtaatgaaggtcagctgtcttttgttcaggaccagtttactggtaaaggccaggaggttatgctgcaatgggacgcggcgaactctacgaccaatctgtggctgcacgaagcgggtcactcttctgttgactttctcgttcgtatcgttggccagaccgcgcagagcgacattatcgtctaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatat BBa_K1175028_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaggggagagagccctgccatgaacgcgtctagcgaacgtgaccgttctctgtttggtgtgctgcgtcagttccgccgctccttttggtctgttggcatcttcagcgcggtcatcaatgttctgatgctggcacctagcgtctacatgctccaggtttacgaccgtgtcctcgccagcggcaacggtattactctgctgatgctcacgctcctgatggcgggtctgtgcgccttcatgggtgctctcgaatgggttcgcagcctgctggtagttcgtctcggtactcgcatcgacctcgcgctcaatcaagacgtttttaacgcggctttcgcgcgtaatctggaagctggtgatggccgtgccggtctggcgctgaccgacctcaccctgctccgtcaattcatcacgggtaatgcactgttcgcgttcttcgacgttccgtggttcccgctctttctcctcgtactgttcctgctgcacccttggctcggcatgctcgcactgggtggtacggttgtacctggtggcgtaggcctcgcagaaccggcgtctgaccagtctaccgcgggtggctctaatcaacaaagccaacaagccactcatctcgcggatgcgcagctccgtaatgcggacgttatcgaagcgatgggtatgctcggtaacctccgtcgtcgctggctggcgcgtcactaccgctttatctccctccaaaatctcgcttccgagcgcgcagccgccgttggtggtgcgtccaaatactctcgtattgcactccaaagcctcatgctgggcctcggtgcgctcctcgcgatcgacggcaaaatcacgccgggtatgatgattgcgggctctatcctcgtcggtcgtgttctgtccccgattgaccagctgatcggtgtttggaaacagtggtcttctgcccgcattgcgtggcagcgcctgacccgtctgattgccgcatatccgccacgccctgcggctatggctctcccagcgccggaaggccacctgtccgttgagcaagtttctctgcgtaccgcacaaggcaatactcgcctgcaaaacatccatttctccctgcaagcgggtgagaccctggttatcctgggcgcctctggttctggcaaatcttctctcgctcgtctgctcgtgggtgcacaatcccctactcaaggtaaagttcgcctcgatggtgcggacctgaaccaagttgacaaaaacaccttcggtccgaccattggttacctcccgcaggacgttcaactgttcaaaggtagcctggcggaaaacattgcccgtttcggcgacgccgacccggaaaaggttgttgcggcagctaagctcgccggtgttcacgaactcatcctgtctctgcctaatggttacgacactgagctgggtgacggcggtggcggcctgtctggtggtcaacgtcagcgtatcggtctcgcacgcgcaatgtacggcgacccgtgtctcctcattctggacgaaccgaacgcatctctggactctgagggtgaccaagcactcatgcaggctattgtggcgctccagaaacgtggtgcaaccgttgtactcattacgcaccgtccggcactcaccaccctcgctcagaaaatcctgatcctccacgagggtcaacagcaacgtatgggcctggcccgtgacgttctgacggaactccagcagcgctccgcggcaaaccaggcgcgtatgaacccgaccgcagcaatgccacagtaataatactagagagcagggatctcgtgatgactggtatggatattaccactcaggacgaactcaacgaagctgccatgcgtgaccgtgcgagccgtgacgaagaacgtgcgctccgtctgggttggtggctcgtcctcgcgggtttcggtggtttcctcctgtgggcgctcctggctccactggacaagggtgttgcggttcagggtaacgttgtagtttccggtaaccgtaaagttatccagcacatgcaaggcggtattgttgaccgcatccaagttaaggacggcgaccgtgttgccgctggtcaggttctgctcactctcaatgcagtcgacgctcgtaccacctccgagggcctgggttctcagtacgatcagctcatcgcgcgtgaagcgcgcctcctcgccgagcagcgtaaccagtcttctctcgctgcaacgccacgcctgacgcaggcacgccagcgtccggaaatggcggcgattatcgccctccaggaagacctgctgcgctctcgtcagcagtctctcaaactggaaattgacggtgtccgtgcatctatcgatggtctggaaacttctctgggtgctctgcaaaaagtgatgtcttctaaacagtctgaacaagcgactctgtctcaacagctgcaaggtctgcgtccgctggccgcagacaactacgttccgcgtaacaagatgctcgaaaccgaacgtctcttcgcccaggtttctggcgagctggcccagacctctggtgaagttggtcgtacccgtcgtgacattcaacaacagaaactccgcattgcgcagcgccagcaggagtacgacaaagaagtcaactctgaactgtctgacgttcaggccaaactgaacgaagtaatctctcagcgtgaaaaggcggacttcaatctcgctaacgtgcaggttcgtgcgccggtggcgggcactgtggtggatatgaaaatcttcaccgaaggtggcgtaattgctccgggtcaagttatgatggacattgttccggaggatcagccactgctggtagatggccgtatcccggttgagatggttgacaaagtatggagcggtctgccggtcgaactgcaatttactgcgttttcccaatctactacgccgcgtgtccctggtactgtaaccctgctgagcgcggatcgcctggtggacgaaaaagatggcaccccttactatggcctccgtattcaggtgtctgaggaaggtaaacgtagcctccacggcctggagattaaaccgggtatgcctgtccaaggttttgtgcgtacgggcgaacgctctttcatcaactacctcttcaaaccgctcatggaccgtatgcacctcgcactgaccgaagaataatactagagagcggaaggaagcaatgcgccgtaaagcagttctgctcactgtggttctgagcctgtctggtggtagcgcccaagcgatgggtctgctggacgcgtgggaactcgcgctccgcaatgatgcgcaactgcgtgcggcaggtttcgaacgtgacgcgggtcaagaggaagttgcgattggtcgcgcaggcctcctgccgagcctgcaatacacgtacggtgcgaactactcccactccaaagttacccagcgcgatcgtaccctgaacaacaccacgaaacgtgattatgataactacgtttctacgctgaccctgcgtcagccactcctggactacgcggcatgggcgcgttatcagcaaggcgtaactcgtaaactgatggcggaccagcgttttcgcgaccgttcccaggatctgatggttcgtctgtaccagtcctggagcgaagccctgctggcgcaggaaaagctgatgctcctcgacgctcagcgtcgtgcataccaggaacagctcgcactgaatcgccgtctcctggctgccggtgagggcacgcaaaccgacctgcgtgagaccgaagcacgttataccgtcaccgaggcgcagcgtatcgaacaggaggacaccctcgatgccgccatgactgacctggaaaacatgatgggctctcctctccagatccaagacctgtctccactggctctcgacactctcccggataacgtaacggaaaaccgtagcctctctcagtggcgtgaactcacggtacgtcacaacgccaaactggcagtacaacgtgagaacgtggattactctcgctacgaaatcgagcgcaaccgtgcgggccacctcccgaccctggacctggttgcctctacccgtaattccctctccgagagcgaatacaactacaatcaaaaatacgacacccagaccgtaggtctgcaagttcgtgttcctctctactctggcggtgcggtttctgcgtctatgcgccaagcggcagctgagtatcaacagtcccaggcggaactggacaatcagactcgtcagaccttcgcggagctgcgtcgccagttcaacctgtgcgcaaacggtgctgcgaaaatccgtgcttggcagatgtctgtagctgctgccgaagaggccattcgtgcgacccgtcagtctgttgcgggtggtgaacgtattaacctggatgtgctcatggctgagcaggaatggtataatgcccgtcgtgagctgacggaggttaagtaccgttggctgcaagcgtggctcaatctgcgctacacggctggtactctcaacgaacaagatatgatgcagctggcagcctggttccagtctgcgccggttatcaataaaaccggcatcaatgctgccacgggcaacaagaccaattaa BBa_K215100_sequence 1 aggggagagagccctgccatgaacgcgtctagcgaacgtgaccgttctctgtttggtgtgctgcgtcagttccgccgctccttttggtctgttggcatcttcagcgcggtcatcaatgttctgatgctggcacctagcgtctacatgctccaggtttacgaccgtgtcctcgccagcggcaacggtattactctgctgatgctcacgctcctgatggcgggtctgtgcgccttcatgggtgctctcgaatgggttcgcagcctgctggtagttcgtctcggtactcgcatcgacctcgcgctcaatcaagacgtttttaacgcggctttcgcgcgtaatctggaagctggtgatggccgtgccggtctggcgctgaccgacctcaccctgctccgtcaattcatcacgggtaatgcactgttcgcgttcttcgacgttccgtggttcccgctctttctcctcgtactgttcctgctgcacccttggctcggcatgctcgcactgggtggtacggttgtacctggtggcgtaggcctcgcagaaccggcgtctgaccagtctaccgcgggtggctctaatcaacaaagccaacaagccactcatctcgcggatgcgcagctccgtaatgcggacgttatcgaagcgatgggtatgctcggtaacctccgtcgtcgctggctggcgcgtcactaccgctttatctccctccaaaatctcgcttccgagcgcgcagccgccgttggtggtgcgtccaaatactctcgtattgcactccaaagcctcatgctgggcctcggtgcgctcctcgcgatcgacggcaaaatcacgccgggtatgatgattgcgggctctatcctcgtcggtcgtgttctgtccccgattgaccagctgatcggtgtttggaaacagtggtcttctgcccgcattgcgtggcagcgcctgacccgtctgattgccgcatatccgccacgccctgcggctatggctctcccagcgccggaaggccacctgtccgttgagcaagtttctctgcgtaccgcacaaggcaatactcgcctgcaaaacatccatttctccctgcaagcgggtgagaccctggttatcctgggcgcctctggttctggcaaatcttctctcgctcgtctgctcgtgggtgcacaatcccctactcaaggtaaagttcgcctcgatggtgcggacctgaaccaagttgacaaaaacaccttcggtccgaccattggttacctcccgcaggacgttcaactgttcaaaggtagcctggcggaaaacattgcccgtttcggcgacgccgacccggaaaaggttgttgcggcagctaagctcgccggtgttcacgaactcatcctgtctctgcctaatggttacgacactgagctgggtgacggcggtggcggcctgtctggtggtcaacgtcagcgtatcggtctcgcacgcgcaatgtacggcgacccgtgtctcctcattctggacgaaccgaacgcatctctggactctgagggtgaccaagcactcatgcaggctattgtggcgctccagaaacgtggtgcaaccgttgtactcattacgcaccgtccggcactcaccaccctcgctcagaaaatcctgatcctccacgagggtcaacagcaacgtatgggcctggcccgtgacgttctgacggaactccagcagcgctccgcggcaaaccaggcgcgtatgaacccgaccgcagcaatgccacagtaataa BBa_K1175007_sequence 1 atgagaaaactgtatcatggcgcttgctattatccggaattatgggatgaagagacgattcagcaggacattgacatcatgcgtgaagttggcgtaaatgttgtgcggatcggcgaatttgcctggtcagtcatggaacctgaagaaggaaaaattgacgtcggttttttcaaagaaatcatcgcccggctgtatgatagcgggatcgaaacgattatgtgcacgccgacgcctaccccgccgatttggttctcacatggccggcccgaacgcatgcatgccaatgaaaaaagagagatcatggggcatggctcccgtcagcatgcctgtacgaacaacccgtatttccgaaaaaaagccgccatcatcaccacagccatcgccaaggagcttggccggctcccggggctgatcggatggcagctagacaatgagtttaaatgccatgttgcagaatgcatgtgtgagacatgcttgcgcctatggcatgactggctcaaaaatcgctacggggtaattgagcgcttgaatgaagcttggggaaccgatgtgtggagcgagacctatcagacgtttgagcaagtcccgcagccgggaccggccccgtttctgcatcatgcctctctacgcactatgtatcagctgttttcgatggagatgatcgcttcgtttgcggatgaacaggccaaaatcatccgctgctattcagatgcgccgatcacgcataacggatcagtcatgttcagcgtggacaatgagcgaatgtttcagaatctcgattttgcctcctatgacacgtacgcttcgcaggaaaacgcctctgcctttttattgaactgtgatttatggagaaatctgaaacaagggcgcccgttttggattttggaaacgagtccgtcgtatgccgcctcgcttgaaagctccgcttacccgcacgcagacgggtatttgcaggccgaagccgtatcgtcctacgccttagggagccaggggttttgctactggctatggcgacagcagcgttcaggcagcgagatttcccacggttcggttctcagtgcctggggcgaacccaccatcggctatcaaaatgtgctggcggttgagcgggcaagaaaggaaatcgagcctattattctatcgactgaacccgttcaagccgaggcggcgatgacttactctgacagagcaaaagcatttattaaaactgagcctcaccggggactccggcatcgttcgcttgtgacgcatttttatgaacgtattctcaacacggggattcaccgtgaccttattccggaaggcgctccactggacggctatcgcttgctgtttacgccatttgtgccgtatttgtcttctgaatttatcaaaaaagcttcggcattcgctgaagcgggcggcatctggatcaccgggccgctgacaggaggacgcacatgcgagcataccattcataccgattgcggacttggcgaacttgagaaaacgtcagggatcaaaacactttttacctttccgatgaatgagaacgtgaatacaggaaaagcgtttggcatcacggcgccgctcggactgtggagcgcggtgtttgacacagagagcggaaacacccttggcacggttgaagcaggaccgggggcgggccatgcttttctgacggaacggaattacggcgaggggaaaattgtcatgctgggctcgcttccatccgggaaagaaggggatgcgatgctggaagcgctcgtcaggcattatgcggaggaagctgttatttccagccggtcggatgtgacacccggcacgatcgttgccccgcgtataggcgaaaacggccttgtgtggatcgttgtgaatatggatggaaaaggcgggagcgtgacattgccggaatcgggaacggatttgttgacgcaccgcttggaaaaggcggggagactggcggtcggaccgcatgaataccgtgtgattcaatttgacaatcacagctaataa BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K314200_sequence 1 atgtcctacgactacgagaaaaccagcctcaccctctaccgggcggtattcaaggccaactacgacggcgacgtcggtcgctacctgcatcccgacaaggaactcgccgaggctgcggaagtcgccccgctgctgcatccgaccttcgacagccccaacacccctggcgtccccgcccgcgcgccggacatcgtcgccggccgcgacggcctctacgccccggacaccggcggcacctcggtgttcgaccgcgccggcgtgctgcgccgcgccgacggcgacttcgtgatacccgacggcaccgacatcccgccggaccttaaggtgaagcaggacagctacaacaagcgcctgcaagccacccactacaccatcatgccggccaagccgatgtaccgggaggtcctcatgggccaactggacaacttcgtgcgcaacgccatccgccgccaatgggaaaaagcccgcgggctctag BBa_K215101_sequence 1 agcagggatctcgtgatgactggtatggatattaccactcaggacgaactcaacgaagctgccatgcgtgaccgtgcgagccgtgacgaagaacgtgcgctccgtctgggttggtggctcgtcctcgcgggtttcggtggtttcctcctgtgggcgctcctggctccactggacaagggtgttgcggttcagggtaacgttgtagtttccggtaaccgtaaagttatccagcacatgcaaggcggtattgttgaccgcatccaagttaaggacggcgaccgtgttgccgctggtcaggttctgctcactctcaatgcagtcgacgctcgtaccacctccgagggcctgggttctcagtacgatcagctcatcgcgcgtgaagcgcgcctcctcgccgagcagcgtaaccagtcttctctcgctgcaacgccacgcctgacgcaggcacgccagcgtccggaaatggcggcgattatcgccctccaggaagacctgctgcgctctcgtcagcagtctctcaaactggaaattgacggtgtccgtgcatctatcgatggtctggaaacttctctgggtgctctgcaaaaagtgatgtcttctaaacagtctgaacaagcgactctgtctcaacagctgcaaggtctgcgtccgctggccgcagacaactacgttccgcgtaacaagatgctcgaaaccgaacgtctcttcgcccaggtttctggcgagctggcccagacctctggtgaagttggtcgtacccgtcgtgacattcaacaacagaaactccgcattgcgcagcgccagcaggagtacgacaaagaagtcaactctgaactgtctgacgttcaggccaaactgaacgaagtaatctctcagcgtgaaaaggcggacttcaatctcgctaacgtgcaggttcgtgcgccggtggcgggcactgtggtggatatgaaaatcttcaccgaaggtggcgtaattgctccgggtcaagttatgatggacattgttccggaggatcagccactgctggtagatggccgtatcccggttgagatggttgacaaagtatggagcggtctgccggtcgaactgcaatttactgcgttttcccaatctactacgccgcgtgtccctggtactgtaaccctgctgagcgcggatcgcctggtggacgaaaaagatggcaccccttactatggcctccgtattcaggtgtctgaggaaggtaaacgtagcctccacggcctggagattaaaccgggtatgcctgtccaaggttttgtgcgtacgggcgaacgctctttcatcaactacctcttcaaaccgctcatggaccgtatgcacctcgcactgaccgaagaataa BBa_K875002_sequence 1 aatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaattgtgagcggataacaatttcacaca BBa_B0031_sequence 1 tcacacaggaaacc BBa_K215104_sequence 1 aggggagagagccctgccatgaacgcgtctagcgaacgtgaccgttctctgtttggtgtgctgcgtcagttccgccgctccttttggtctgttggcatcttcagcgcggtcatcaatgttctgatgctggcacctagcgtctacatgctccaggtttacgaccgtgtcctcgccagcggcaacggtattactctgctgatgctcacgctcctgatggcgggtctgtgcgccttcatgggtgctctcgaatgggttcgcagcctgctggtagttcgtctcggtactcgcatcgacctcgcgctcaatcaagacgtttttaacgcggctttcgcgcgtaatctggaagctggtgatggccgtgccggtctggcgctgaccgacctcaccctgctccgtcaattcatcacgggtaatgcactgttcgcgttcttcgacgttccgtggttcccgctctttctcctcgtactgttcctgctgcacccttggctcggcatgctcgcactgggtggtacggttgtacctggtggcgtaggcctcgcagaaccggcgtctgaccagtctaccgcgggtggctctaatcaacaaagccaacaagccactcatctcgcggatgcgcagctccgtaatgcggacgttatcgaagcgatgggtatgctcggtaacctccgtcgtcgctggctggcgcgtcactaccgctttatctccctccaaaatctcgcttccgagcgcgcagccgccgttggtggtgcgtccaaatactctcgtattgcactccaaagcctcatgctgggcctcggtgcgctcctcgcgatcgacggcaaaatcacgccgggtatgatgattgcgggctctatcctcgtcggtcgtgttctgtccccgattgaccagctgatcggtgtttggaaacagtggtcttctgcccgcattgcgtggcagcgcctgacccgtctgattgccgcatatccgccacgccctgcggctatggctctcccagcgccggaaggccacctgtccgttgagcaagtttctctgcgtaccgcacaaggcaatactcgcctgcaaaacatccatttctccctgcaagcgggtgagaccctggttatcctgggcgcctctggttctggcaaatcttctctcgctcgtctgctcgtgggtgcacaatcccctactcaaggtaaagttcgcctcgatggtgcggacctgaaccaagttgacaaaaacaccttcggtccgaccattggttacctcccgcaggacgttcaactgttcaaaggtagcctggcggaaaacattgcccgtttcggcgacgccgacccggaaaaggttgttgcggcagctaagctcgccggtgttcacgaactcatcctgtctctgcctaatggttacgacactgagctgggtgacggcggtggcggcctgtctggtggtcaacgtcagcgtatcggtctcgcacgcgcaatgtacggcgacccgtgtctcctcattctggacgaaccgaacgcatctctggactctgagggtgaccaagcactcatgcaggctattgtggcgctccagaaacgtggtgcaaccgttgtactcattacgcaccgtccggcactcaccaccctcgctcagaaaatcctgatcctccacgagggtcaacagcaacgtatgggcctggcccgtgacgttctgacggaactccagcagcgctccgcggcaaaccaggcgcgtatgaacccgaccgcagcaatgccacagtaataatactagagagcagggatctcgtgatgactggtatggatattaccactcaggacgaactcaacgaagctgccatgcgtgaccgtgcgagccgtgacgaagaacgtgcgctccgtctgggttggtggctcgtcctcgcgggtttcggtggtttcctcctgtgggcgctcctggctccactggacaagggtgttgcggttcagggtaacgttgtagtttccggtaaccgtaaagttatccagcacatgcaaggcggtattgttgaccgcatccaagttaaggacggcgaccgtgttgccgctggtcaggttctgctcactctcaatgcagtcgacgctcgtaccacctccgagggcctgggttctcagtacgatcagctcatcgcgcgtgaagcgcgcctcctcgccgagcagcgtaaccagtcttctctcgctgcaacgccacgcctgacgcaggcacgccagcgtccggaaatggcggcgattatcgccctccaggaagacctgctgcgctctcgtcagcagtctctcaaactggaaattgacggtgtccgtgcatctatcgatggtctggaaacttctctgggtgctctgcaaaaagtgatgtcttctaaacagtctgaacaagcgactctgtctcaacagctgcaaggtctgcgtccgctggccgcagacaactacgttccgcgtaacaagatgctcgaaaccgaacgtctcttcgcccaggtttctggcgagctggcccagacctctggtgaagttggtcgtacccgtcgtgacattcaacaacagaaactccgcattgcgcagcgccagcaggagtacgacaaagaagtcaactctgaactgtctgacgttcaggccaaactgaacgaagtaatctctcagcgtgaaaaggcggacttcaatctcgctaacgtgcaggttcgtgcgccggtggcgggcactgtggtggatatgaaaatcttcaccgaaggtggcgtaattgctccgggtcaagttatgatggacattgttccggaggatcagccactgctggtagatggccgtatcccggttgagatggttgacaaagtatggagcggtctgccggtcgaactgcaatttactgcgttttcccaatctactacgccgcgtgtccctggtactgtaaccctgctgagcgcggatcgcctggtggacgaaaaagatggcaccccttactatggcctccgtattcaggtgtctgaggaaggtaaacgtagcctccacggcctggagattaaaccgggtatgcctgtccaaggttttgtgcgtacgggcgaacgctctttcatcaactacctcttcaaaccgctcatggaccgtatgcacctcgcactgaccgaagaataatactagagagcggaaggaagcaatgcgccgtaaagcagttctgctcactgtggttctgagcctgtctggtggtagcgcccaagcgatgggtctgctggacgcgtgggaactcgcgctccgcaatgatgcgcaactgcgtgcggcaggtttcgaacgtgacgcgggtcaagaggaagttgcgattggtcgcgcaggcctcctgccgagcctgcaatacacgtacggtgcgaactactcccactccaaagttacccagcgcgatcgtaccctgaacaacaccacgaaacgtgattatgataactacgtttctacgctgaccctgcgtcagccactcctggactacgcggcatgggcgcgttatcagcaaggcgtaactcgtaaactgatggcggaccagcgttttcgcgaccgttcccaggatctgatggttcgtctgtaccagtcctggagcgaagccctgctggcgcaggaaaagctgatgctcctcgacgctcagcgtcgtgcataccaggaacagctcgcactgaatcgccgtctcctggctgccggtgagggcacgcaaaccgacctgcgtgagaccgaagcacgttataccgtcaccgaggcgcagcgtatcgaacaggaggacaccctcgatgccgccatgactgacctggaaaacatgatgggctctcctctccagatccaagacctgtctccactggctctcgacactctcccggataacgtaacggaaaaccgtagcctctctcagtggcgtgaactcacggtacgtcacaacgccaaactggcagtacaacgtgagaacgtggattactctcgctacgaaatcgagcgcaaccgtgcgggccacctcccgaccctggacctggttgcctctacccgtaattccctctccgagagcgaatacaactacaatcaaaaatacgacacccagaccgtaggtctgcaagttcgtgttcctctctactctggcggtgcggtttctgcgtctatgcgccaagcggcagctgagtatcaacagtcccaggcggaactggacaatcagactcgtcagaccttcgcggagctgcgtcgccagttcaacctgtgcgcaaacggtgctgcgaaaatccgtgcttggcagatgtctgtagctgctgccgaagaggccattcgtgcgacccgtcagtctgttgcgggtggtgaacgtattaacctggatgtgctcatggctgagcaggaatggtataatgcccgtcgtgagctgacggaggttaagtaccgttggctgcaagcgtggctcaatctgcgctacacggctggtactctcaacgaacaagatatgatgcagctggcagcctggttccagtctgcgccggttatcaataaaaccggcatcaatgctgccacgggcaacaagaccaattaa BBa_K206000_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc BBa_K875004_sequence 1 aatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagaaagaggagaaatactaatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggagatattctgctgacccagagcccggcgattctgagcgtgagcccgggcgaacgcgtgagctttagctgccgcgcgagccagagcattggcgtgctgtgcgatcgcattagcattcattggtatcagcagcgcaccaacggcagcccgcgcctgctgattaaatatgcgagcgaaagcattagcggcattccgagccgctttagcggcagcggcagcggcaccgattttaccctgagcattaacagcgtggaaagcgaagatattgcggattattattgccagcagagcaacagctggccgtggacctttggcggcggcaccaaactggaaattaaaggcggcaaaggcagcggcggcaaaggcaccggcggcaaaggcagcggcggcaaaggcagcgaagtgaaactggtggaaagcggcggcgatctggtgcagccgggcggcagcctgaaactgagctgcgcggtgcattgcgatcgcaccagcggctttacctttagcgattattatatgtattgggtgcgccagaccccggaaaaacgcctggaatgggtggcgtatattagcagcgtgcattgcgatcgcggcggcggcagcaccttttatccagataccgtgaaaggccgctttaccattagccgcgataacgcgaaaaacaccctgtatctacagatgagccgcctgaaaagcgatgataccgcgatgtattattgcgcgcgcgcgtatcatggcaactattttgattattggggccagggcaccaccctgaccgtgagcagctccggtcaccaccaccaccaccactgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgctcaccttcgggtgggcctttctgcgtttata BBa_K875008_sequence 1 aatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaattgtgagcggataacaatttcacacatactagagtcacacaggaaacctactagatgtcctacgactacgagaaaaccagcctcaccctctaccgggcggtattcaaggccaactacgacggcgacgtcggtcgctacctgcatcccgacaaggaactcgccgaggctgcggaagtcgccccgctgctgcatccgaccttcgacagccccaacacccctggcgtccccgcccgcgcgccggacatcgtcgccggccgcgacggcctctacgccccggacaccggcggcacctcggtgttcgaccgcgccggcgtgctgcgccgcgccgacggcgacttcgtgatacccgacggcaccgacatcccgccggaccttaaggtgaagcaggacagctacaacaagcgcctgcaagccacccactacaccatcatgccggccaagccgatgtaccgggaggtcctcatgggccaactggacaacttcgtgcgcaacgccatccgccgccaatgggaaaaagcccgcgggctctagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z