LOCUS BBa_K1175400 8367 bp DNA linear 26-Dec-2024 DEFINITION Antinorovirus antibody, Antihorizontal transfer mechanism, Purification and secretion tag, tripartat ACCESSION BBa_K1175400 VERSION BBa_K1175400.1 FEATURES Location/Qualifiers promoter 1..131 /label=promoter misc_feature 40..57 /label=AraI1 misc_feature 61..78 /label=AraI2 promoter 1..130 /label=BBa_K206000 RBS 141..156 /label=native RBS CDS 157..1887 /label=prtD CDS 139..1887 /label=BBa_K215100 RBS 1898..1910 /label=native rbs CDS 1911..3257 /label=prtE CDS 1896..3257 /label=BBa_K215101 RBS 3268..3279 /label=Native RBS CDS 3280..4668 /label=prtF CDS 3266..4668 /label=BBa_K215102 misc_feature 139..4668 /label=BBa_K215104 stem_loop 4688..4731 /label=stem_loop misc_feature 4677..4756 /label=BBa_B0010 terminator 4677..4756 /label=BBa_B0010 stem_loop 4772..4791 /label=T7 TE misc_feature 4798..4798 /label=stop polyA_site 4792..4805 /label=polya misc_feature 4765..4805 /label=BBa_B0012 terminator 4765..4805 /label=BBa_B0012 terminator 4677..4805 /label=BBa_B0015 promoter 4814..4944 /label=promoter misc_feature 4853..4870 /label=AraI1 misc_feature 4874..4891 /label=AraI2 promoter 4814..4943 /label=BBa_K206000 misc_feature 4956..4959 /label=conserved RBS 4952..4963 /label=BBa_B0034 misc_feature 4970..5017 /label=nanotag misc_feature 5018..5026 /label=GSA linker misc_feature 5027..5044 /label=His tag misc_feature 5045..5053 /label=GSA linker misc_feature 5054..5073 /label=TEV protease site misc_feature 5074..5083 /label=GSA linker misc_feature 5084..5089 /label=NheI misc_feature 5090..5098 /label=GSA linker misc_feature 5099..5119 /label=TEV protease site misc_feature 5120..5128 /label=GSA linker misc_feature 5129..5146 /label=His tag misc_feature 5147..5155 /label=GSA linker misc_feature 5156..5704 /label=Secretion tag misc_feature 5705..5834 /label=terminator (B0015) misc_feature 4970..5834 /label=BBa_K215001 stem_loop 5854..5897 /label=stem_loop misc_feature 5843..5922 /label=BBa_B0010 terminator 5843..5922 /label=BBa_B0010 stem_loop 5938..5957 /label=T7 TE misc_feature 5964..5964 /label=stop polyA_site 5958..5971 /label=polya misc_feature 5931..5971 /label=BBa_B0012 terminator 5931..5971 /label=BBa_B0012 terminator 5843..5971 /label=BBa_B0015 RBS 6068..6079 /label=rbs stem_loop 7368..7495 /label=stem_loop promoter 5980..6059 /label=promoter misc_feature 7339..7356 /label=tag CDS 6080..7332 /label=protein misc_feature 5980..7495 /label=BBa_K875004 stem_loop 7515..7558 /label=stem_loop misc_feature 7504..7583 /label=BBa_B0010 terminator 7504..7583 /label=BBa_B0010 stem_loop 7599..7618 /label=T7 TE misc_feature 7625..7625 /label=stop polyA_site 7619..7632 /label=polya misc_feature 7592..7632 /label=BBa_B0012 terminator 7592..7632 /label=BBa_B0012 terminator 7504..7632 /label=BBa_B0015 promoter 7641..7725 /label=BBa_K875001 misc_feature 7740..7743 /label=conserved RBS 7734..7747 /label=BBa_B0031 misc_feature 7754..8230 /label=tse2 CDS 7754..8230 /label=BBa_K314200 stem_loop 8250..8293 /label=stem_loop misc_feature 8239..8318 /label=BBa_B0010 terminator 8239..8318 /label=BBa_B0010 stem_loop 8334..8353 /label=T7 TE misc_feature 8360..8360 /label=stop polyA_site 8354..8367 /label=polya misc_feature 8327..8367 /label=BBa_B0012 terminator 8327..8367 /label=BBa_B0012 terminator 8239..8367 /label=BBa_B0015 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagag gggagagagc cctgccatga acgcgtctag cgaacgtgac 181 cgttctctgt ttggtgtgct gcgtcagttc cgccgctcct tttggtctgt tggcatcttc 241 agcgcggtca tcaatgttct gatgctggca cctagcgtct acatgctcca ggtttacgac 301 cgtgtcctcg ccagcggcaa cggtattact ctgctgatgc tcacgctcct gatggcgggt 361 ctgtgcgcct tcatgggtgc tctcgaatgg gttcgcagcc tgctggtagt tcgtctcggt 421 actcgcatcg acctcgcgct caatcaagac gtttttaacg cggctttcgc gcgtaatctg 481 gaagctggtg atggccgtgc cggtctggcg ctgaccgacc tcaccctgct ccgtcaattc 541 atcacgggta atgcactgtt cgcgttcttc gacgttccgt ggttcccgct ctttctcctc 601 gtactgttcc tgctgcaccc ttggctcggc atgctcgcac tgggtggtac ggttgtacct 661 ggtggcgtag gcctcgcaga accggcgtct gaccagtcta ccgcgggtgg ctctaatcaa 721 caaagccaac aagccactca tctcgcggat gcgcagctcc gtaatgcgga cgttatcgaa 781 gcgatgggta tgctcggtaa cctccgtcgt cgctggctgg cgcgtcacta ccgctttatc 841 tccctccaaa atctcgcttc cgagcgcgca gccgccgttg gtggtgcgtc caaatactct 901 cgtattgcac tccaaagcct catgctgggc ctcggtgcgc tcctcgcgat cgacggcaaa 961 atcacgccgg gtatgatgat tgcgggctct atcctcgtcg gtcgtgttct gtccccgatt 1021 gaccagctga tcggtgtttg gaaacagtgg tcttctgccc gcattgcgtg gcagcgcctg 1081 acccgtctga ttgccgcata tccgccacgc cctgcggcta tggctctccc agcgccggaa 1141 ggccacctgt ccgttgagca agtttctctg cgtaccgcac aaggcaatac tcgcctgcaa 1201 aacatccatt tctccctgca agcgggtgag accctggtta tcctgggcgc ctctggttct 1261 ggcaaatctt ctctcgctcg tctgctcgtg ggtgcacaat cccctactca aggtaaagtt 1321 cgcctcgatg gtgcggacct gaaccaagtt gacaaaaaca ccttcggtcc gaccattggt 1381 tacctcccgc aggacgttca actgttcaaa ggtagcctgg cggaaaacat tgcccgtttc 1441 ggcgacgccg acccggaaaa ggttgttgcg gcagctaagc tcgccggtgt tcacgaactc 1501 atcctgtctc tgcctaatgg ttacgacact gagctgggtg acggcggtgg cggcctgtct 1561 ggtggtcaac gtcagcgtat cggtctcgca cgcgcaatgt acggcgaccc gtgtctcctc 1621 attctggacg aaccgaacgc atctctggac tctgagggtg accaagcact catgcaggct 1681 attgtggcgc tccagaaacg tggtgcaacc gttgtactca ttacgcaccg tccggcactc 1741 accaccctcg ctcagaaaat cctgatcctc cacgagggtc aacagcaacg tatgggcctg 1801 gcccgtgacg ttctgacgga actccagcag cgctccgcgg caaaccaggc gcgtatgaac 1861 ccgaccgcag caatgccaca gtaataatac tagagagcag ggatctcgtg atgactggta 1921 tggatattac cactcaggac gaactcaacg aagctgccat gcgtgaccgt gcgagccgtg 1981 acgaagaacg tgcgctccgt ctgggttggt ggctcgtcct cgcgggtttc ggtggtttcc 2041 tcctgtgggc gctcctggct ccactggaca agggtgttgc ggttcagggt aacgttgtag 2101 tttccggtaa ccgtaaagtt atccagcaca tgcaaggcgg tattgttgac cgcatccaag 2161 ttaaggacgg cgaccgtgtt gccgctggtc aggttctgct cactctcaat gcagtcgacg 2221 ctcgtaccac ctccgagggc ctgggttctc agtacgatca gctcatcgcg cgtgaagcgc 2281 gcctcctcgc cgagcagcgt aaccagtctt ctctcgctgc aacgccacgc ctgacgcagg 2341 cacgccagcg tccggaaatg gcggcgatta tcgccctcca ggaagacctg ctgcgctctc 2401 gtcagcagtc tctcaaactg gaaattgacg gtgtccgtgc atctatcgat ggtctggaaa 2461 cttctctggg tgctctgcaa aaagtgatgt cttctaaaca gtctgaacaa gcgactctgt 2521 ctcaacagct gcaaggtctg cgtccgctgg ccgcagacaa ctacgttccg cgtaacaaga 2581 tgctcgaaac cgaacgtctc ttcgcccagg tttctggcga gctggcccag acctctggtg 2641 aagttggtcg tacccgtcgt gacattcaac aacagaaact ccgcattgcg cagcgccagc 2701 aggagtacga caaagaagtc aactctgaac tgtctgacgt tcaggccaaa ctgaacgaag 2761 taatctctca gcgtgaaaag gcggacttca atctcgctaa cgtgcaggtt cgtgcgccgg 2821 tggcgggcac tgtggtggat atgaaaatct tcaccgaagg tggcgtaatt gctccgggtc 2881 aagttatgat ggacattgtt ccggaggatc agccactgct ggtagatggc cgtatcccgg 2941 ttgagatggt tgacaaagta tggagcggtc tgccggtcga actgcaattt actgcgtttt 3001 cccaatctac tacgccgcgt gtccctggta ctgtaaccct gctgagcgcg gatcgcctgg 3061 tggacgaaaa agatggcacc ccttactatg gcctccgtat tcaggtgtct gaggaaggta 3121 aacgtagcct ccacggcctg gagattaaac cgggtatgcc tgtccaaggt tttgtgcgta 3181 cgggcgaacg ctctttcatc aactacctct tcaaaccgct catggaccgt atgcacctcg 3241 cactgaccga agaataatac tagagagcgg aaggaagcaa tgcgccgtaa agcagttctg 3301 ctcactgtgg ttctgagcct gtctggtggt agcgcccaag cgatgggtct gctggacgcg 3361 tgggaactcg cgctccgcaa tgatgcgcaa ctgcgtgcgg caggtttcga acgtgacgcg 3421 ggtcaagagg aagttgcgat tggtcgcgca ggcctcctgc cgagcctgca atacacgtac 3481 ggtgcgaact actcccactc caaagttacc cagcgcgatc gtaccctgaa caacaccacg 3541 aaacgtgatt atgataacta cgtttctacg ctgaccctgc gtcagccact cctggactac 3601 gcggcatggg cgcgttatca gcaaggcgta actcgtaaac tgatggcgga ccagcgtttt 3661 cgcgaccgtt cccaggatct gatggttcgt ctgtaccagt cctggagcga agccctgctg 3721 gcgcaggaaa agctgatgct cctcgacgct cagcgtcgtg cataccagga acagctcgca 3781 ctgaatcgcc gtctcctggc tgccggtgag ggcacgcaaa ccgacctgcg tgagaccgaa 3841 gcacgttata ccgtcaccga ggcgcagcgt atcgaacagg aggacaccct cgatgccgcc 3901 atgactgacc tggaaaacat gatgggctct cctctccaga tccaagacct gtctccactg 3961 gctctcgaca ctctcccgga taacgtaacg gaaaaccgta gcctctctca gtggcgtgaa 4021 ctcacggtac gtcacaacgc caaactggca gtacaacgtg agaacgtgga ttactctcgc 4081 tacgaaatcg agcgcaaccg tgcgggccac ctcccgaccc tggacctggt tgcctctacc 4141 cgtaattccc tctccgagag cgaatacaac tacaatcaaa aatacgacac ccagaccgta 4201 ggtctgcaag ttcgtgttcc tctctactct ggcggtgcgg tttctgcgtc tatgcgccaa 4261 gcggcagctg agtatcaaca gtcccaggcg gaactggaca atcagactcg tcagaccttc 4321 gcggagctgc gtcgccagtt caacctgtgc gcaaacggtg ctgcgaaaat ccgtgcttgg 4381 cagatgtctg tagctgctgc cgaagaggcc attcgtgcga cccgtcagtc tgttgcgggt 4441 ggtgaacgta ttaacctgga tgtgctcatg gctgagcagg aatggtataa tgcccgtcgt 4501 gagctgacgg aggttaagta ccgttggctg caagcgtggc tcaatctgcg ctacacggct 4561 ggtactctca acgaacaaga tatgatgcag ctggcagcct ggttccagtc tgcgccggtt 4621 atcaataaaa ccggcatcaa tgctgccacg ggcaacaaga ccaattaata ctagagccag 4681 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 4741 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 4801 ttatatacta gagacattga ttatttgcac ggcgtcacac tttgctatgc catagcaaga 4861 tagtccataa gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct 4921 ccataccgtt tttttgggct agctactaga gaaagaggag aaatactaga tggacgttga 4981 agcatggctg ggtgcccgtg taccgctggt tgaaacgggc agcgcacatc accaccatca 5041 tcatggttcc gccgaaaacc tctactttca gggcggctct gctgctagcg gttctgccga 5101 gaatctgtac ttccaaggtg gctccgctca tcaccaccat catcacggta gcgcaggcgg 5161 cactgacacc tttgacttct ctggttactc taacaatcag cgtatcaacc tgaacgaggg 5221 ttctttctct gacgttggtg gtctgaaggg caacgtttct atcgcgcatg gtgttaccat 5281 cgaaaacgcg atcggtggtt ctggtaacga catcctgatc ggtaatggcg cggataacat 5341 cctccagggt ggtgcgggtg acgacgttct gtacggttct acgggtgcgg acacgctgac 5401 gggtggcgct ggtcgtgaca ttttcgtata tggtagcggt caagactcta ccgtttctgc 5461 atacgactgg atcacggatt ttcagacggg tatcgacaaa atcgacctgt ccgctttccg 5521 taatgaaggt cagctgtctt ttgttcagga ccagtttact ggtaaaggcc aggaggttat 5581 gctgcaatgg gacgcggcga actctacgac caatctgtgg ctgcacgaag cgggtcactc 5641 ttctgttgac tttctcgttc gtatcgttgg ccagaccgcg cagagcgaca ttatcgtcta 5701 ataaccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 5761 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 5821 ttctgcgttt atattactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 5881 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 5941 tcaccttcgg gtgggccttt ctgcgtttat atactagaga atcataaaaa atttatttgc 6001 tttgtgagcg gataacaatt ataatagatt caattgtgag cggataacaa tttcacacat 6061 actagagaaa gaggagaaat actagaaaga ggagaaatac taatgaaagc tactaaactg 6121 gtactgggcg cggtaatcct gggttctact ctgctggcag gttgctccag caacgctaaa 6181 atcgatcagg gaattaaccc gtatgttggc tttgaaatgg gttacgactg gttaggtcgt 6241 atgccgtaca aaggcagcgt tgaaaacggt gcatacaaag ctcagggcgt tcaactgacc 6301 gctaaactgg gttacccaat cactgacgac ctggacatct acactcgtct gggtggcatg 6361 gtatggcgtg cagacactaa atccaacgtt tatggtaaaa accacgacac cggcgtttct 6421 ccggtcttcg ctggcggtgt tgagtacgcg atcactcctg aaatcgctac ccgtctggaa 6481 taccagtgga ccaacaacat cggtgacgca cacaccatcg gcactcgtcc ggacaacggc 6541 ggaggttctg gagatattct gctgacccag agcccggcga ttctgagcgt gagcccgggc 6601 gaacgcgtga gctttagctg ccgcgcgagc cagagcattg gcgtgctgtg cgatcgcatt 6661 agcattcatt ggtatcagca gcgcaccaac ggcagcccgc gcctgctgat taaatatgcg 6721 agcgaaagca ttagcggcat tccgagccgc tttagcggca gcggcagcgg caccgatttt 6781 accctgagca ttaacagcgt ggaaagcgaa gatattgcgg attattattg ccagcagagc 6841 aacagctggc cgtggacctt tggcggcggc accaaactgg aaattaaagg cggcaaaggc 6901 agcggcggca aaggcaccgg cggcaaaggc agcggcggca aaggcagcga agtgaaactg 6961 gtggaaagcg gcggcgatct ggtgcagccg ggcggcagcc tgaaactgag ctgcgcggtg 7021 cattgcgatc gcaccagcgg ctttaccttt agcgattatt atatgtattg ggtgcgccag 7081 accccggaaa aacgcctgga atgggtggcg tatattagca gcgtgcattg cgatcgcggc 7141 ggcggcagca ccttttatcc agataccgtg aaaggccgct ttaccattag ccgcgataac 7201 gcgaaaaaca ccctgtatct acagatgagc cgcctgaaaa gcgatgatac cgcgatgtat 7261 tattgcgcgc gcgcgtatca tggcaactat tttgattatt ggggccaggg caccaccctg 7321 accgtgagca gctccggtca ccaccaccac caccactgat actagagcca ggcatcaaat 7381 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 7441 cgctctctac tagagtcaca ctgctcacct tcgggtgggc ctttctgcgt ttatatacta 7501 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 7561 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 7621 tctgcgttta tatactagag aaatcataaa aaatttattt gctttgtgag cggataacaa 7681 ttataataga ttcaacaaac agacaatctg gtctgtttgt attattacta gagtcacaca 7741 ggaaacctac tagatgtcct acgactacga gaaaaccagc ctcaccctct accgggcggt 7801 attcaaggcc aactacgacg gcgacgtcgg tcgctacctg catcccgaca aggaactcgc 7861 cgaggctgcg gaagtcgccc cgctgctgca tccgaccttc gacagcccca acacccctgg 7921 cgtccccgcc cgcgcgccgg acatcgtcgc cggccgcgac ggcctctacg ccccggacac 7981 cggcggcacc tcggtgttcg accgcgccgg cgtgctgcgc cgcgccgacg gcgacttcgt 8041 gatacccgac ggcaccgaca tcccgccgga ccttaaggtg aagcaggaca gctacaacaa 8101 gcgcctgcaa gccacccact acaccatcat gccggccaag ccgatgtacc gggaggtcct 8161 catgggccaa ctggacaact tcgtgcgcaa cgccatccgc cgccaatggg aaaaagcccg 8221 cgggctctag tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 8281 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 8341 cttcgggtgg gcctttctgc gtttata //