##gff-version 3 ##sequence-region BBa_K1175400 1 8367 BBa_K1175400 . engineered_region 139 4668 . + 0 ID=BBa_K215104;Name=BBa_K215104 BBa_K1175400 . CDS 3266 4668 . + 0 ID=BBa_K215102;Name=BBa_K215102;Parent=BBa_K215104 BBa_K1175400 . ribosome_entry_site 3268 3279 . + 0 ID=annotation2027449;Name=Native RBS;Parent=BBa_K215102 BBa_K1175400 . CDS 3280 4668 . + 0 ID=annotation2027450;Name=prtF;Parent=BBa_K215102 BBa_K1175400 . CDS 139 1887 . + 0 ID=BBa_K215100;Name=BBa_K215100;Parent=BBa_K215104 BBa_K1175400 . ribosome_entry_site 141 156 . + 0 ID=annotation2027445;Name=native RBS;Parent=BBa_K215100 BBa_K1175400 . CDS 157 1887 . + 0 ID=annotation2027446;Name=prtD;Parent=BBa_K215100 BBa_K1175400 . CDS 1896 3257 . + 0 ID=BBa_K215101;Name=BBa_K215101;Parent=BBa_K215104 BBa_K1175400 . CDS 1911 3257 . + 0 ID=annotation2027448;Name=prtE;Parent=BBa_K215101 BBa_K1175400 . ribosome_entry_site 1898 1910 . + 0 ID=annotation2027447;Name=native rbs;Parent=BBa_K215101 BBa_K1175400 . ribosome_entry_site 7734 7747 . + 0 ID=BBa_B0031;Name=BBa_B0031 BBa_K1175400 . conserved_region 7740 7743 . + 0 ID=annotation23316;Name=conserved;Parent=BBa_B0031 BBa_K1175400 . promoter 7641 7725 . + 0 ID=BBa_K875001;Name=BBa_K875001 BBa_K1175400 . terminator 7504 7632 . + 0 ID=BBa_B0015;Name=BBa_B0015 BBa_K1175400 . terminator 7504 7583 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K1175400 . engineered_region 7504 7583 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K1175400 . stem_loop 7515 7558 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K1175400 . terminator 7592 7632 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K1175400 . stop_codon 7625 7625 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K1175400 . polyA_site 7619 7632 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K1175400 . stem_loop 7599 7618 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K1175400 . engineered_region 7592 7632 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K1175400 . engineered_region 5980 7495 . + 0 ID=BBa_K875004;Name=BBa_K875004 BBa_K1175400 . tag 7339 7356 . + 0 ID=annotation2194273;Name=tag;Parent=BBa_K875004 BBa_K1175400 . stem_loop 7368 7495 . + 0 ID=annotation2194270;Name=stem_loop;Parent=BBa_K875004 BBa_K1175400 . ribosome_entry_site 6068 6079 . + 0 ID=annotation2194268;Name=rbs;Parent=BBa_K875004 BBa_K1175400 . promoter 5980 6059 . + 0 ID=annotation2194271;Name=promoter;Parent=BBa_K875004 BBa_K1175400 . CDS 6080 7332 . + 0 ID=annotation2194279;Name=protein;Parent=BBa_K875004 BBa_K1175400 . ribosome_entry_site 4952 4963 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_K1175400 . conserved_region 4956 4959 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K1175400 . terminator 8239 8367 . + 0 ID=BBa_B0015;Name=BBa_B0015 BBa_K1175400 . terminator 8239 8318 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K1175400 . engineered_region 8239 8318 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K1175400 . stem_loop 8250 8293 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K1175400 . terminator 8327 8367 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K1175400 . stop_codon 8360 8360 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K1175400 . polyA_site 8354 8367 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K1175400 . stem_loop 8334 8353 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K1175400 . engineered_region 8327 8367 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K1175400 . CDS 7754 8230 . + 0 ID=BBa_K314200;Name=BBa_K314200 BBa_K1175400 . engineered_region 7754 8230 . + 0 ID=annotation2089456;Name=tse2;Parent=BBa_K314200 BBa_K1175400 . promoter 1 130 . + 0 ID=BBa_K206000;Name=BBa_K206000 BBa_K1175400 . promoter 1 131 . + 0 ID=annotation2049252;Name=promoter;Parent=BBa_K206000 BBa_K1175400 . operator 61 78 . + 0 ID=annotation2049254;Name=AraI2;Parent=BBa_K206000 BBa_K1175400 . operator 40 57 . + 0 ID=annotation2049253;Name=AraI1;Parent=BBa_K206000 BBa_K1175400 . terminator 4677 4805 . + 0 ID=BBa_B0015;Name=BBa_B0015 BBa_K1175400 . terminator 4677 4756 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K1175400 . engineered_region 4677 4756 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K1175400 . stem_loop 4688 4731 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K1175400 . terminator 4765 4805 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K1175400 . stop_codon 4798 4798 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K1175400 . polyA_site 4792 4805 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K1175400 . stem_loop 4772 4791 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K1175400 . engineered_region 4765 4805 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K1175400 . tag 4970 5834 . + 0 ID=BBa_K215001;Name=BBa_K215001 BBa_K1175400 . sequence_feature 5147 5155 . + 0 ID=annotation2027598;Name=GSA linker;Parent=BBa_K215001 BBa_K1175400 . sequence_feature 5054 5073 . + 0 ID=annotation2027591;Name=TEV protease site;Parent=BBa_K215001 BBa_K1175400 . tag 5129 5146 . + 0 ID=annotation2027597;Name=His tag;Parent=BBa_K215001 BBa_K1175400 . sequence_feature 5084 5089 . + 0 ID=annotation2027593;Name=NheI;Parent=BBa_K215001 BBa_K1175400 . sequence_feature 5018 5026 . + 0 ID=annotation2027588;Name=GSA linker;Parent=BBa_K215001 BBa_K1175400 . sequence_feature 5120 5128 . + 0 ID=annotation2027596;Name=GSA linker;Parent=BBa_K215001 BBa_K1175400 . sequence_feature 5090 5098 . + 0 ID=annotation2027594;Name=GSA linker;Parent=BBa_K215001 BBa_K1175400 . tag 5027 5044 . + 0 ID=annotation2027589;Name=His tag;Parent=BBa_K215001 BBa_K1175400 . tag 5156 5704 . + 0 ID=annotation2027599;Name=Secretion tag;Parent=BBa_K215001 BBa_K1175400 . sequence_feature 5099 5119 . + 0 ID=annotation2027595;Name=TEV protease site;Parent=BBa_K215001 BBa_K1175400 . sequence_feature 5045 5053 . + 0 ID=annotation2027590;Name=GSA linker;Parent=BBa_K215001 BBa_K1175400 . sequence_feature 5074 5083 . + 0 ID=annotation2027592;Name=GSA linker;Parent=BBa_K215001 BBa_K1175400 . stop_codon 5705 5834 . + 0 ID=annotation2027600;Name=terminator (B0015);Parent=BBa_K215001 BBa_K1175400 . tag 4970 5017 . + 0 ID=annotation2027587;Name=nanotag;Parent=BBa_K215001 BBa_K1175400 . terminator 5843 5971 . + 0 ID=BBa_B0015;Name=BBa_B0015 BBa_K1175400 . terminator 5843 5922 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K1175400 . engineered_region 5843 5922 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K1175400 . stem_loop 5854 5897 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K1175400 . terminator 5931 5971 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K1175400 . stop_codon 5964 5964 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K1175400 . polyA_site 5958 5971 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K1175400 . stem_loop 5938 5957 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K1175400 . engineered_region 5931 5971 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K1175400 . promoter 4814 4943 . + 0 ID=BBa_K206000;Name=BBa_K206000 BBa_K1175400 . promoter 4814 4944 . + 0 ID=annotation2049252;Name=promoter;Parent=BBa_K206000 BBa_K1175400 . operator 4874 4891 . + 0 ID=annotation2049254;Name=AraI2;Parent=BBa_K206000 BBa_K1175400 . operator 4853 4870 . + 0 ID=annotation2049253;Name=AraI1;Parent=BBa_K206000 >BBa_K1175400 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacg ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaggggagagagccctgccat gaacgcgtctagcgaacgtgaccgttctctgtttggtgtgctgcgtcagttccgccgctccttttggtctgttggcatc ttcagcgcggtcatcaatgttctgatgctggcacctagcgtctacatgctccaggtttacgaccgtgtcctcgccagcg gcaacggtattactctgctgatgctcacgctcctgatggcgggtctgtgcgccttcatgggtgctctcgaatgggttcg cagcctgctggtagttcgtctcggtactcgcatcgacctcgcgctcaatcaagacgtttttaacgcggctttcgcgcgt aatctggaagctggtgatggccgtgccggtctggcgctgaccgacctcaccctgctccgtcaattcatcacgggtaatg cactgttcgcgttcttcgacgttccgtggttcccgctctttctcctcgtactgttcctgctgcacccttggctcggcat gctcgcactgggtggtacggttgtacctggtggcgtaggcctcgcagaaccggcgtctgaccagtctaccgcgggtggc tctaatcaacaaagccaacaagccactcatctcgcggatgcgcagctccgtaatgcggacgttatcgaagcgatgggta tgctcggtaacctccgtcgtcgctggctggcgcgtcactaccgctttatctccctccaaaatctcgcttccgagcgcgc agccgccgttggtggtgcgtccaaatactctcgtattgcactccaaagcctcatgctgggcctcggtgcgctcctcgcg atcgacggcaaaatcacgccgggtatgatgattgcgggctctatcctcgtcggtcgtgttctgtccccgattgaccagc tgatcggtgtttggaaacagtggtcttctgcccgcattgcgtggcagcgcctgacccgtctgattgccgcatatccgcc acgccctgcggctatggctctcccagcgccggaaggccacctgtccgttgagcaagtttctctgcgtaccgcacaaggc aatactcgcctgcaaaacatccatttctccctgcaagcgggtgagaccctggttatcctgggcgcctctggttctggca aatcttctctcgctcgtctgctcgtgggtgcacaatcccctactcaaggtaaagttcgcctcgatggtgcggacctgaa ccaagttgacaaaaacaccttcggtccgaccattggttacctcccgcaggacgttcaactgttcaaaggtagcctggcg gaaaacattgcccgtttcggcgacgccgacccggaaaaggttgttgcggcagctaagctcgccggtgttcacgaactca tcctgtctctgcctaatggttacgacactgagctgggtgacggcggtggcggcctgtctggtggtcaacgtcagcgtat cggtctcgcacgcgcaatgtacggcgacccgtgtctcctcattctggacgaaccgaacgcatctctggactctgagggt gaccaagcactcatgcaggctattgtggcgctccagaaacgtggtgcaaccgttgtactcattacgcaccgtccggcac tcaccaccctcgctcagaaaatcctgatcctccacgagggtcaacagcaacgtatgggcctggcccgtgacgttctgac ggaactccagcagcgctccgcggcaaaccaggcgcgtatgaacccgaccgcagcaatgccacagtaataatactagaga gcagggatctcgtgatgactggtatggatattaccactcaggacgaactcaacgaagctgccatgcgtgaccgtgcgag ccgtgacgaagaacgtgcgctccgtctgggttggtggctcgtcctcgcgggtttcggtggtttcctcctgtgggcgctc ctggctccactggacaagggtgttgcggttcagggtaacgttgtagtttccggtaaccgtaaagttatccagcacatgc aaggcggtattgttgaccgcatccaagttaaggacggcgaccgtgttgccgctggtcaggttctgctcactctcaatgc agtcgacgctcgtaccacctccgagggcctgggttctcagtacgatcagctcatcgcgcgtgaagcgcgcctcctcgcc gagcagcgtaaccagtcttctctcgctgcaacgccacgcctgacgcaggcacgccagcgtccggaaatggcggcgatta tcgccctccaggaagacctgctgcgctctcgtcagcagtctctcaaactggaaattgacggtgtccgtgcatctatcga tggtctggaaacttctctgggtgctctgcaaaaagtgatgtcttctaaacagtctgaacaagcgactctgtctcaacag ctgcaaggtctgcgtccgctggccgcagacaactacgttccgcgtaacaagatgctcgaaaccgaacgtctcttcgccc aggtttctggcgagctggcccagacctctggtgaagttggtcgtacccgtcgtgacattcaacaacagaaactccgcat tgcgcagcgccagcaggagtacgacaaagaagtcaactctgaactgtctgacgttcaggccaaactgaacgaagtaatc tctcagcgtgaaaaggcggacttcaatctcgctaacgtgcaggttcgtgcgccggtggcgggcactgtggtggatatga aaatcttcaccgaaggtggcgtaattgctccgggtcaagttatgatggacattgttccggaggatcagccactgctggt agatggccgtatcccggttgagatggttgacaaagtatggagcggtctgccggtcgaactgcaatttactgcgttttcc caatctactacgccgcgtgtccctggtactgtaaccctgctgagcgcggatcgcctggtggacgaaaaagatggcaccc cttactatggcctccgtattcaggtgtctgaggaaggtaaacgtagcctccacggcctggagattaaaccgggtatgcc tgtccaaggttttgtgcgtacgggcgaacgctctttcatcaactacctcttcaaaccgctcatggaccgtatgcacctc gcactgaccgaagaataatactagagagcggaaggaagcaatgcgccgtaaagcagttctgctcactgtggttctgagc ctgtctggtggtagcgcccaagcgatgggtctgctggacgcgtgggaactcgcgctccgcaatgatgcgcaactgcgtg cggcaggtttcgaacgtgacgcgggtcaagaggaagttgcgattggtcgcgcaggcctcctgccgagcctgcaatacac gtacggtgcgaactactcccactccaaagttacccagcgcgatcgtaccctgaacaacaccacgaaacgtgattatgat aactacgtttctacgctgaccctgcgtcagccactcctggactacgcggcatgggcgcgttatcagcaaggcgtaactc gtaaactgatggcggaccagcgttttcgcgaccgttcccaggatctgatggttcgtctgtaccagtcctggagcgaagc cctgctggcgcaggaaaagctgatgctcctcgacgctcagcgtcgtgcataccaggaacagctcgcactgaatcgccgt ctcctggctgccggtgagggcacgcaaaccgacctgcgtgagaccgaagcacgttataccgtcaccgaggcgcagcgta tcgaacaggaggacaccctcgatgccgccatgactgacctggaaaacatgatgggctctcctctccagatccaagacct gtctccactggctctcgacactctcccggataacgtaacggaaaaccgtagcctctctcagtggcgtgaactcacggta cgtcacaacgccaaactggcagtacaacgtgagaacgtggattactctcgctacgaaatcgagcgcaaccgtgcgggcc acctcccgaccctggacctggttgcctctacccgtaattccctctccgagagcgaatacaactacaatcaaaaatacga cacccagaccgtaggtctgcaagttcgtgttcctctctactctggcggtgcggtttctgcgtctatgcgccaagcggca gctgagtatcaacagtcccaggcggaactggacaatcagactcgtcagaccttcgcggagctgcgtcgccagttcaacc tgtgcgcaaacggtgctgcgaaaatccgtgcttggcagatgtctgtagctgctgccgaagaggccattcgtgcgacccg tcagtctgttgcgggtggtgaacgtattaacctggatgtgctcatggctgagcaggaatggtataatgcccgtcgtgag ctgacggaggttaagtaccgttggctgcaagcgtggctcaatctgcgctacacggctggtactctcaacgaacaagata tgatgcagctggcagcctggttccagtctgcgccggttatcaataaaaccggcatcaatgctgccacgggcaacaagac caattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacattg attatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgcttttt atcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatggacgt tgaagcatggctgggtgcccgtgtaccgctggttgaaacgggcagcgcacatcaccaccatcatcatggttccgccgaa aacctctactttcagggcggctctgctgctagcggttctgccgagaatctgtacttccaaggtggctccgctcatcacc accatcatcacggtagcgcaggcggcactgacacctttgacttctctggttactctaacaatcagcgtatcaacctgaa cgagggttctttctctgacgttggtggtctgaagggcaacgtttctatcgcgcatggtgttaccatcgaaaacgcgatc ggtggttctggtaacgacatcctgatcggtaatggcgcggataacatcctccagggtggtgcgggtgacgacgttctgt acggttctacgggtgcggacacgctgacgggtggcgctggtcgtgacattttcgtatatggtagcggtcaagactctac cgtttctgcatacgactggatcacggattttcagacgggtatcgacaaaatcgacctgtccgctttccgtaatgaaggt cagctgtcttttgttcaggaccagtttactggtaaaggccaggaggttatgctgcaatgggacgcggcgaactctacga ccaatctgtggctgcacgaagcgggtcactcttctgttgactttctcgttcgtatcgttggccagaccgcgcagagcga cattatcgtctaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatattactagagccag gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac tagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaatcataaaaaatttatttgctttg tgagcggataacaattataatagattcaattgtgagcggataacaatttcacacatactagagaaagaggagaaatact agaaagaggagaaatactaatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggt tgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgta tgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaat cactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaa aaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctgg aataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggagatat tctgctgacccagagcccggcgattctgagcgtgagcccgggcgaacgcgtgagctttagctgccgcgcgagccagagc attggcgtgctgtgcgatcgcattagcattcattggtatcagcagcgcaccaacggcagcccgcgcctgctgattaaat atgcgagcgaaagcattagcggcattccgagccgctttagcggcagcggcagcggcaccgattttaccctgagcattaa cagcgtggaaagcgaagatattgcggattattattgccagcagagcaacagctggccgtggacctttggcggcggcacc aaactggaaattaaaggcggcaaaggcagcggcggcaaaggcaccggcggcaaaggcagcggcggcaaaggcagcgaag tgaaactggtggaaagcggcggcgatctggtgcagccgggcggcagcctgaaactgagctgcgcggtgcattgcgatcg caccagcggctttacctttagcgattattatatgtattgggtgcgccagaccccggaaaaacgcctggaatgggtggcg tatattagcagcgtgcattgcgatcgcggcggcggcagcaccttttatccagataccgtgaaaggccgctttaccatta gccgcgataacgcgaaaaacaccctgtatctacagatgagccgcctgaaaagcgatgataccgcgatgtattattgcgc gcgcgcgtatcatggcaactattttgattattggggccagggcaccaccctgaccgtgagcagctccggtcaccaccac caccaccactgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt tgtttgtcggtgaacgctctctactagagtcacactgctcaccttcgggtgggcctttctgcgtttatatactagagcc aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct actagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaaatcataaaaaatttatttgct ttgtgagcggataacaattataatagattcaacaaacagacaatctggtctgtttgtattattactagagtcacacagg aaacctactagatgtcctacgactacgagaaaaccagcctcaccctctaccgggcggtattcaaggccaactacgacgg cgacgtcggtcgctacctgcatcccgacaaggaactcgccgaggctgcggaagtcgccccgctgctgcatccgaccttc gacagccccaacacccctggcgtccccgcccgcgcgccggacatcgtcgccggccgcgacggcctctacgccccggaca ccggcggcacctcggtgttcgaccgcgccggcgtgctgcgccgcgccgacggcgacttcgtgatacccgacggcaccga catcccgccggaccttaaggtgaagcaggacagctacaacaagcgcctgcaagccacccactacaccatcatgccggcc aagccgatgtaccgggaggtcctcatgggccaactggacaacttcgtgcgcaacgccatccgccgccaatgggaaaaag cccgcgggctctagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata