BBa_K1175400
type: ComponentDefinition
persistent identity: BBa K1175400
display id: BBa_K1175400
version: 1
was generated by: iGEM to SBOL conversion
description: Antinorovirus antibody, Antihorizontal transfer mechanism, Purification and secretion tag, tripartat
created: 2013-09-26T11:00:00Z
modified: 2015-05-08T01:09:35Z
mutable provenance: Parts were received from the iGEM registry and Team Trieste
owned by: james
owned by: myers
mutable description: This combinatory part is a self contained secretion system that combines a secretion and purification tag, a tripartite pump that is specific to the sec tag, a antinorovirus particle like antibody, Tse2 toxin mediated horizontal gene transfer mechanism. Tripartite SecI pump These are the three essential parts prtD, prtE, prtF, that make up the Type I secretion system from Erwinia chrysanthemi.This part is made of 3 genes: prtD, prtE, and prtF, that constitute a type I Erwinia chrysanthemi secretion system. The Pump is expressed from a strong constitutive promoter, BBa_k206000, and has the translational terminator BBa_B0014. In pSB1C3. Used to secrete proteins containing prtB C-terminal tag. The prtB C-terminal tag is built into the protein generator [BBa_K1175012]. Any protein of interest can be inserted into the protein generator and then secreted when used in conjunction with this secretion system. Secretion and purification tag This part BBa_K1175012 A composite part formed between BBa_K206000 strong pBAD promoter, BBa_B0034 strong RBS, and BBa_K215001 a purification and secretion tag; a secretion system designed by Washington iGEM team 2009 BBa_K215001. This tag can be used to secrete proteins of interest by inserting them into a specific NheI cut site flanked by C terminus His tag and an N terminus Secretion tag. Both tags can be removed by a TEV protease. The secretion tag is also specific to the tripartite pump. Antinorovirus like particle antibody This construct BBa_K875004 is designed for the expression of an already described engineered antinorovirus (NoV) monoclonal antibody (mAb 54.6) in fusion with LPP-OmpA. The antibody is expressed in a single chain fragment variable (scFv) format containing light (VL) and heavy (VH) variable domains separeted by a flexible peptide linker. It has already been reported that the scFv 54.6 binds a native recombinant NoV particles (VLPs) and inhibits VLP interaction with cells. LPP-OmpA functions as a leader sequence and …
discontinued: false
dominant: false
group u list: _1488_
m user id: 0
owner id: 14098
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Standard Assembly
star: false
creator: Nicholas K. Goldner
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K206000
component: BBa_B0015
component: BBa_K215001
component: BBa_B0034
component: BBa_K215104
component: BBa_K314200
component: BBa_B0031
component: BBa_K875004
component: BBa_K875001
sequence annotation: BBa_K206000
sequence annotation: BBa_K215104
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_K215001
sequence annotation: BBa_K875004
sequence annotation: BBa_K875001
sequence annotation: BBa_B0031
sequence annotation: BBa_K314200
sequence: https://synbiohub.org/public/igem/BBa_K1175400_sequence/1

BBa_K206000
type: Component
persistent identity: component2364837
display id: component2364837
version: 1
top level: BBa_K1175400
definition: pBAD
access: public

BBa_B0015
type: Component
persistent identity: component2364833
display id: component2364833
version: 1
top level: BBa_K1175400
definition: BBa_B0015
access: public

BBa_K215001
type: Component
persistent identity: component2364854
display id: component2364854
version: 1
top level: BBa_K1175400
definition: BBa_K215001
access: public

BBa_B0034
type: Component
persistent identity: component2364839
display id: component2364839
version: 1
top level: BBa_K1175400
definition: BBa_B0034
access: public

BBa_K215104
type: Component
persistent identity: component2364826
display id: component2364826
version: 1
top level: BBa_K1175400
definition: BBa_K215104
access: public

BBa_K206000
type: Component
persistent identity: component2364816
display id: component2364816
version: 1
top level: BBa_K1175400
definition: pBAD
access: public

BBa_K314200
type: Component
persistent identity: component2364879
display id: component2364879
version: 1
top level: BBa_K1175400
definition: Tse2
access: public

BBa_B0031
type: Component
persistent identity: component2364877
display id: component2364877
version: 1
top level: BBa_K1175400
definition: BBa_B0031
access: public

BBa_B0015
type: Component
persistent identity: component2364886
display id: component2364886
version: 1
top level: BBa_K1175400
definition: BBa_B0015
access: public

BBa_K875004
type: Component
persistent identity: component2364867
display id: component2364867
version: 1
top level: BBa_K1175400
definition: BBa_K875004
access: public

BBa_B0015
type: Component
persistent identity: component2364861
display id: component2364861
version: 1
top level: BBa_K1175400
definition: BBa_B0015
access: public

BBa_K875001
type: Component
persistent identity: component2364875
display id: component2364875
version: 1
top level: BBa_K1175400
definition: BBa_K875001
access: public

BBa_B0015
type: Component
persistent identity: component2364874
display id: component2364874
version: 1
top level: BBa_K1175400
definition: BBa_B0015
access: public

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2364816
display id: annotation2364816
version: 1
top level: BBa_K1175400
location: https://synbiohub.org/public/igem/BBa_K1175400/annotation2364816/range2364816/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K1175400/annotation2364816/range2364816/1
type: Range
persistent identity: range2364816
display id: range2364816
version: 1
top level: BBa_K1175400
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_K215104
type: SequenceAnnotation
persistent identity: annotation2364826
display id: annotation2364826
version: 1
top level: BBa_K1175400
location: https://synbiohub.org/public/igem/BBa_K1175400/annotation2364826/range2364826/1
component: BBa_K215104

https://synbiohub.org/public/igem/BBa_K1175400/annotation2364826/range2364826/1
type: Range
persistent identity: range2364826
display id: range2364826
version: 1
top level: BBa_K1175400
direction: direction/reverse
start: 139
end: 4668
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2364833
display id: annotation2364833
version: 1
top level: BBa_K1175400
location: https://synbiohub.org/public/igem/BBa_K1175400/annotation2364833/range2364833/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1175400/annotation2364833/range2364833/1
type: Range
persistent identity: range2364833
display id: range2364833
version: 1
top level: BBa_K1175400
direction: direction/reverse
start: 4677
end: 4805
orientation: inline

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2364837
display id: annotation2364837
version: 1
top level: BBa_K1175400
location: https://synbiohub.org/public/igem/BBa_K1175400/annotation2364837/range2364837/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K1175400/annotation2364837/range2364837/1
type: Range
persistent identity: range2364837
display id: range2364837
version: 1
top level: BBa_K1175400
direction: direction/reverse
start: 4814
end: 4943
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2364839
display id: annotation2364839
version: 1
top level: BBa_K1175400
location: https://synbiohub.org/public/igem/BBa_K1175400/annotation2364839/range2364839/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1175400/annotation2364839/range2364839/1
type: Range
persistent identity: range2364839
display id: range2364839
version: 1
top level: BBa_K1175400
direction: direction/reverse
start: 4952
end: 4963
orientation: inline

BBa_K215001
type: SequenceAnnotation
persistent identity: annotation2364854
display id: annotation2364854
version: 1
top level: BBa_K1175400
location: https://synbiohub.org/public/igem/BBa_K1175400/annotation2364854/range2364854/1
component: BBa_K215001

https://synbiohub.org/public/igem/BBa_K1175400/annotation2364854/range2364854/1
type: Range
persistent identity: range2364854
display id: range2364854
version: 1
top level: BBa_K1175400
direction: direction/reverse
start: 4970
end: 5834
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2364861
display id: annotation2364861
version: 1
top level: BBa_K1175400
location: https://synbiohub.org/public/igem/BBa_K1175400/annotation2364861/range2364861/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1175400/annotation2364861/range2364861/1
type: Range
persistent identity: range2364861
display id: range2364861
version: 1
top level: BBa_K1175400
direction: direction/reverse
start: 5843
end: 5971
orientation: inline

BBa_K875004
type: SequenceAnnotation
persistent identity: annotation2364867
display id: annotation2364867
version: 1
top level: BBa_K1175400
location: https://synbiohub.org/public/igem/BBa_K1175400/annotation2364867/range2364867/1
component: BBa_K875004

https://synbiohub.org/public/igem/BBa_K1175400/annotation2364867/range2364867/1
type: Range
persistent identity: range2364867
display id: range2364867
version: 1
top level: BBa_K1175400
direction: direction/reverse
start: 5980
end: 7495
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2364874
display id: annotation2364874
version: 1
top level: BBa_K1175400
location: https://synbiohub.org/public/igem/BBa_K1175400/annotation2364874/range2364874/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1175400/annotation2364874/range2364874/1
type: Range
persistent identity: range2364874
display id: range2364874
version: 1
top level: BBa_K1175400
direction: direction/reverse
start: 7504
end: 7632
orientation: inline

BBa_K875001
type: SequenceAnnotation
persistent identity: annotation2364875
display id: annotation2364875
version: 1
top level: BBa_K1175400
location: https://synbiohub.org/public/igem/BBa_K1175400/annotation2364875/range2364875/1
component: BBa_K875001

https://synbiohub.org/public/igem/BBa_K1175400/annotation2364875/range2364875/1
type: Range
persistent identity: range2364875
display id: range2364875
version: 1
top level: BBa_K1175400
direction: direction/reverse
start: 7641
end: 7725
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation2364877
display id: annotation2364877
version: 1
top level: BBa_K1175400
location: https://synbiohub.org/public/igem/BBa_K1175400/annotation2364877/range2364877/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_K1175400/annotation2364877/range2364877/1
type: Range
persistent identity: range2364877
display id: range2364877
version: 1
top level: BBa_K1175400
direction: direction/reverse
start: 7734
end: 7747
orientation: inline

BBa_K314200
type: SequenceAnnotation
persistent identity: annotation2364879
display id: annotation2364879
version: 1
top level: BBa_K1175400
location: https://synbiohub.org/public/igem/BBa_K1175400/annotation2364879/range2364879/1
component: BBa_K314200

https://synbiohub.org/public/igem/BBa_K1175400/annotation2364879/range2364879/1
type: Range
persistent identity: range2364879
display id: range2364879
version: 1
top level: BBa_K1175400
direction: direction/reverse
start: 7754
end: 8230
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2364886
display id: annotation2364886
version: 1
top level: BBa_K1175400
location: https://synbiohub.org/public/igem/BBa_K1175400/annotation2364886/range2364886/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1175400/annotation2364886/range2364886/1
type: Range
persistent identity: range2364886
display id: range2364886
version: 1
top level: BBa_K1175400
direction: direction/reverse
start: 8239
end: 8367
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K215104
type: ComponentDefinition
persistent identity: BBa K215104
display id: BBa_K215104
version: 1
was generated by: iGEM to SBOL conversion
description: prtD_prtE_prtF from erwinia chrysanthemi
created: 2009-09-29T11:00:00Z
modified: 2015-05-08T01:11:30Z
mutable provenance: Gene Bank ID: Erwinia chrysanthemi M60395.1
owned by: james
owned by: myers
mutable description: This is the 3 genes: prtD, prtE, prtF, that are from a type I Erwinia chrysanthemi secretion system.
discontinued: false
dominant: false
group u list: _320_
m user id: 0
owner id: 5596
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Synthesized to be bio-bricked, keeps original protein sequence and ribosome binding site.
star: false
creator: Josef Dunbar
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K215102
component: BBa_K215101
component: BBa_K215100
sequence annotation: BBa_K215100
sequence annotation: BBa_K215101
sequence annotation: BBa_K215102
sequence: https://synbiohub.org/public/igem/BBa_K215104_sequence/1

BBa_K215102
type: Component
persistent identity: component2027474
display id: component2027474
version: 1
top level: BBa_K215104
definition: BBa_K215102
access: public

BBa_K215101
type: Component
persistent identity: component2027471
display id: component2027471
version: 1
top level: BBa_K215104
definition: BBa_K215101
access: public

BBa_K215100
type: Component
persistent identity: component2027468
display id: component2027468
version: 1
top level: BBa_K215104
definition: BBa_K215100
access: public

BBa_K215100
type: SequenceAnnotation
persistent identity: annotation2027468
display id: annotation2027468
version: 1
top level: BBa_K215104
location: https://synbiohub.org/public/igem/BBa_K215104/annotation2027468/range2027468/1
component: BBa_K215100

https://synbiohub.org/public/igem/BBa_K215104/annotation2027468/range2027468/1
type: Range
persistent identity: range2027468
display id: range2027468
version: 1
top level: BBa_K215104
direction: direction/reverse
start: 1
end: 1749
orientation: inline

BBa_K215101
type: SequenceAnnotation
persistent identity: annotation2027471
display id: annotation2027471
version: 1
top level: BBa_K215104
location: https://synbiohub.org/public/igem/BBa_K215104/annotation2027471/range2027471/1
component: BBa_K215101

https://synbiohub.org/public/igem/BBa_K215104/annotation2027471/range2027471/1
type: Range
persistent identity: range2027471
display id: range2027471
version: 1
top level: BBa_K215104
direction: direction/reverse
start: 1758
end: 3119
orientation: inline

BBa_K215102
type: SequenceAnnotation
persistent identity: annotation2027474
display id: annotation2027474
version: 1
top level: BBa_K215104
location: https://synbiohub.org/public/igem/BBa_K215104/annotation2027474/range2027474/1
component: BBa_K215102

https://synbiohub.org/public/igem/BBa_K215104/annotation2027474/range2027474/1
type: Range
persistent identity: range2027474
display id: range2027474
version: 1
top level: BBa_K215104
direction: direction/reverse
start: 3128
end: 4530
orientation: inline

BBa_K215001
type: ComponentDefinition
persistent identity: BBa K215001
display id: BBa_K215001
version: 1
was generated by: iGEM to SBOL conversion
description: Secretion Signal and Streptavidin Binding Tags
created: 2009-09-29T11:00:00Z
modified: 2015-05-08T01:11:29Z
mutable provenance: Nanotag (bases 1-48): From Lamla and Erdmann (http://www.ncbi.nlm.nih.gov/pubmed/14680960). Secretion tag (last 181 AA): Gene Bank ID Erwinia chrysanthemi M60395.1
owned by: james
owned by: myers
mutable description: This part is the vector into which afp is inserted to tag it for purification using the IPP system. It contains an NheI restriction site that allows for the insertion of the desired protein coding sequence (NheI is compatible with XbaI and SpeI). This Target Vector part contains two tags: a secretion tag that causes the protein to be exported to the extracellular space, and a nanotag that causes the protein to bind to stretavidin (or some analog). For this part to function properly, it must be combined with a promoter and RBS, and used in E.coli strain UW5alpha, which has two other plasmids containing the other genes required for proper secretion and display of the protein to be purified on the outer membrane.
discontinued: false
dominant: false
experience: experience/None
group u list: _320_
m user id: 0
owner id: 2811
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: NheI was chosen as the protein insertion site because it is compatible with digested XbaI and SpeI restriction sites. The TEV sites and HIS tags flanking the NheI site are present to separate the inserted protein from the added tags. GSA linkers were used to separate the various features of the part.
star: false
creator: Jeff Nivala
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: nanotag
sequence annotation: GSA linker
sequence annotation: His tag
sequence annotation: TEV protease site
sequence annotation: NheI
sequence annotation: Secretion tag
sequence annotation: terminator (B0015)
sequence: https://synbiohub.org/public/igem/BBa_K215001_sequence/1

nanotag
type: SequenceAnnotation
persistent identity: annotation2027587
display id: annotation2027587
version: 1
top level: BBa_K215001
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027587/range2027587/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K215001/annotation2027587/range2027587/1
type: Range
persistent identity: range2027587
display id: range2027587
version: 1
top level: BBa_K215001
direction: direction/forward
start: 1
end: 48
orientation: inline

GSA linker
type: SequenceAnnotation
persistent identity: annotation2027588
display id: annotation2027588
version: 1
top level: BBa_K215001
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027588/range2027588/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K215001/annotation2027588/range2027588/1
type: Range
persistent identity: range2027588
display id: range2027588
version: 1
top level: BBa_K215001
direction: direction/forward
start: 49
end: 57
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2027589
display id: annotation2027589
version: 1
top level: BBa_K215001
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027589/range2027589/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K215001/annotation2027589/range2027589/1
type: Range
persistent identity: range2027589
display id: range2027589
version: 1
top level: BBa_K215001
direction: direction/forward
start: 58
end: 75
orientation: inline

GSA linker
type: SequenceAnnotation
persistent identity: annotation2027590
display id: annotation2027590
version: 1
top level: BBa_K215001
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027590/range2027590/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K215001/annotation2027590/range2027590/1
type: Range
persistent identity: range2027590
display id: range2027590
version: 1
top level: BBa_K215001
direction: direction/forward
start: 76
end: 84
orientation: inline

TEV protease site
type: SequenceAnnotation
persistent identity: annotation2027591
display id: annotation2027591
version: 1
top level: BBa_K215001
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027591/range2027591/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K215001/annotation2027591/range2027591/1
type: Range
persistent identity: range2027591
display id: range2027591
version: 1
top level: BBa_K215001
direction: direction/forward
start: 85
end: 104
orientation: inline

GSA linker
type: SequenceAnnotation
persistent identity: annotation2027592
display id: annotation2027592
version: 1
top level: BBa_K215001
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027592/range2027592/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K215001/annotation2027592/range2027592/1
type: Range
persistent identity: range2027592
display id: range2027592
version: 1
top level: BBa_K215001
direction: direction/forward
start: 105
end: 114
orientation: inline

NheI
type: SequenceAnnotation
persistent identity: annotation2027593
display id: annotation2027593
version: 1
top level: BBa_K215001
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027593/range2027593/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K215001/annotation2027593/range2027593/1
type: Range
persistent identity: range2027593
display id: range2027593
version: 1
top level: BBa_K215001
direction: direction/forward
start: 115
end: 120
orientation: inline

GSA linker
type: SequenceAnnotation
persistent identity: annotation2027594
display id: annotation2027594
version: 1
top level: BBa_K215001
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027594/range2027594/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K215001/annotation2027594/range2027594/1
type: Range
persistent identity: range2027594
display id: range2027594
version: 1
top level: BBa_K215001
direction: direction/forward
start: 121
end: 129
orientation: inline

TEV protease site
type: SequenceAnnotation
persistent identity: annotation2027595
display id: annotation2027595
version: 1
top level: BBa_K215001
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027595/range2027595/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K215001/annotation2027595/range2027595/1
type: Range
persistent identity: range2027595
display id: range2027595
version: 1
top level: BBa_K215001
direction: direction/forward
start: 130
end: 150
orientation: inline

GSA linker
type: SequenceAnnotation
persistent identity: annotation2027596
display id: annotation2027596
version: 1
top level: BBa_K215001
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027596/range2027596/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K215001/annotation2027596/range2027596/1
type: Range
persistent identity: range2027596
display id: range2027596
version: 1
top level: BBa_K215001
direction: direction/forward
start: 151
end: 159
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2027597
display id: annotation2027597
version: 1
top level: BBa_K215001
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027597/range2027597/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K215001/annotation2027597/range2027597/1
type: Range
persistent identity: range2027597
display id: range2027597
version: 1
top level: BBa_K215001
direction: direction/forward
start: 160
end: 177
orientation: inline

GSA linker
type: SequenceAnnotation
persistent identity: annotation2027598
display id: annotation2027598
version: 1
top level: BBa_K215001
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027598/range2027598/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K215001/annotation2027598/range2027598/1
type: Range
persistent identity: range2027598
display id: range2027598
version: 1
top level: BBa_K215001
direction: direction/forward
start: 178
end: 186
orientation: inline

Secretion tag
type: SequenceAnnotation
persistent identity: annotation2027599
display id: annotation2027599
version: 1
top level: BBa_K215001
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027599/range2027599/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K215001/annotation2027599/range2027599/1
type: Range
persistent identity: range2027599
display id: range2027599
version: 1
top level: BBa_K215001
direction: direction/forward
start: 187
end: 735
orientation: inline

terminator (B0015)
type: SequenceAnnotation
persistent identity: annotation2027600
display id: annotation2027600
version: 1
top level: BBa_K215001
location: https://synbiohub.org/public/igem/BBa_K215001/annotation2027600/range2027600/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K215001/annotation2027600/range2027600/1
type: Range
persistent identity: range2027600
display id: range2027600
version: 1
top level: BBa_K215001
direction: direction/forward
start: 736
end: 865
orientation: inline

BBa_K875004
type: ComponentDefinition
persistent identity: BBa K875004
display id: BBa_K875004
version: 1
was generated by: iGEM to SBOL conversion
description: T5LacO-LPP-OmpA-scFv (scFv 54.6 antinorovirus)-6HIS-TT
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:38Z
mutable provenance: DNA synthesis. 1.???Transport and anchoring of 8-lactamase to the external surface of Escherichia coli??? J. A. Francisco, C. F. Earhart and G. Georgiou. Proc. Natl. Acad. Sci. USA Vol. 89, pp. 2713-2717, April 1992 Biochemistry. 2. ???Recombinant norovirus-specific scFv inhibit virus-like particle binding to cellular ligands??? K. Ettayebi and M. E. Hardy. Published: 31 January 2008 in Virology Journal 2008, 5:21?
owned by: james
owned by: myers
mutable description: This construct is designed for the expression of an already descibed engeneered antinorovirus (NoV) monoclonal antibody (mAb 54.6) in fusion with LPP-OmpA. The antibody is expressed in a single chain fragment variable (scFv) format containing light (VL) and heavy (VH) variable domains separeted by a flexible peptide linker. It has already been reported that the scFv 54.6 binds a native recombinant NoV particles (VLPs) and inhibits VLP interaction with cells. LPP-OmpA functions as a leader sequence and an anchor to display the scFv ot the bacterial surface.The construct consistes of T5 Lac Operator (Bba_K875002), ribosomal binding site, LPP-OmpA-scFv 54.6 antinorovirus, Histidine tag (6HIS), Terminator (B0015).
discontinued: false
dominant: false
experience: experience/Works
group u list: _1136_
m user id: 0
owner id: 12664
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: This coding sequence was optimized for the expression in prokaryotic cells. It has a Histidine tag.
star: false
creator: Marija Drikic, Giorgio Gargari
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: promoter
sequence annotation: rbs
sequence annotation: protein
sequence annotation: tag
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_K875004_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2194271
display id: annotation2194271
version: 1
top level: BBa_K875004
location: https://synbiohub.org/public/igem/BBa_K875004/annotation2194271/range2194271/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K875004/annotation2194271/range2194271/1
type: Range
persistent identity: range2194271
display id: range2194271
version: 1
top level: BBa_K875004
direction: direction/forward
start: 1
end: 80
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2194268
display id: annotation2194268
version: 1
top level: BBa_K875004
location: https://synbiohub.org/public/igem/BBa_K875004/annotation2194268/range2194268/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K875004/annotation2194268/range2194268/1
type: Range
persistent identity: range2194268
display id: range2194268
version: 1
top level: BBa_K875004
direction: direction/forward
start: 89
end: 100
orientation: inline

protein
type: SequenceAnnotation
persistent identity: annotation2194279
display id: annotation2194279
version: 1
top level: BBa_K875004
location: https://synbiohub.org/public/igem/BBa_K875004/annotation2194279/range2194279/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K875004/annotation2194279/range2194279/1
type: Range
persistent identity: range2194279
display id: range2194279
version: 1
top level: BBa_K875004
direction: direction/forward
start: 101
end: 1353
orientation: inline

tag
type: SequenceAnnotation
persistent identity: annotation2194273
display id: annotation2194273
version: 1
top level: BBa_K875004
location: https://synbiohub.org/public/igem/BBa_K875004/annotation2194273/range2194273/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K875004/annotation2194273/range2194273/1
type: Range
persistent identity: range2194273
display id: range2194273
version: 1
top level: BBa_K875004
direction: direction/forward
start: 1360
end: 1377
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2194270
display id: annotation2194270
version: 1
top level: BBa_K875004
location: https://synbiohub.org/public/igem/BBa_K875004/annotation2194270/range2194270/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_K875004/annotation2194270/range2194270/1
type: Range
persistent identity: range2194270
display id: range2194270
version: 1
top level: BBa_K875004
direction: direction/forward
start: 1389
end: 1516
orientation: inline

pBAD
type: ComponentDefinition
persistent identity: BBa K206000
display id: BBa_K206000
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD strong
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:23Z
mutable provenance: The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1].
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: pBAD is an E.coli promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: There were no special design considerations.
star: false
creator: Amelia Hardjasa
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence annotation: AraI1
sequence annotation: AraI2
sequence: https://synbiohub.org/public/igem/BBa_K206000_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2049252
display id: annotation2049252
version: 1
top level: pBAD
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1
type: Range
persistent identity: range2049252
display id: range2049252
version: 1
top level: pBAD
direction: direction/forward
start: 1
end: 131
orientation: inline

AraI1
type: SequenceAnnotation
persistent identity: annotation2049253
display id: annotation2049253
version: 1
top level: pBAD
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1
type: Range
persistent identity: range2049253
display id: range2049253
version: 1
top level: pBAD
direction: direction/forward
start: 40
end: 57
orientation: inline

AraI2
type: SequenceAnnotation
persistent identity: annotation2049254
display id: annotation2049254
version: 1
top level: pBAD
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1
type: Range
persistent identity: range2049254
display id: range2049254
version: 1
top level: pBAD
direction: direction/forward
start: 61
end: 78
orientation: inline

BBa_B0031
type: ComponentDefinition
persistent identity: BBa B0031
display id: BBa_B0031
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.2 (weak) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Cho
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0031_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23316
display id: annotation23316
version: 1
top level: BBa_B0031
location: https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
type: Range
persistent identity: range23316
display id: range23316
version: 1
top level: BBa_B0031
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_K875001
type: ComponentDefinition
persistent identity: BBa K875001
display id: BBa_K875001
version: 1
was generated by: iGEM to SBOL conversion
description: T5 Cumate Operator
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:38Z
mutable provenance: 1) The cumate gene-switch: a system for regulated expression in mammalian cells Alaka Mullick, Yan Xu, Ren?? Warren, Maria Koutroumanis, Claire Guilbault, Sophie Broussau, F??lix Malenfant, Lucie Bourget, Linda Lamoureux, Rita Lo, Antoine W Caron, Amelie Pilotte and Bernard Massie ( BMC Biotechnology 2006, 6:43 doi:10.1186/1472-6750-6-43 ) 2. Novel, Versatile, and Tightly Regulated Expression System for Escherichia coli Strains Young J. Choi, Lyne Morel, Teffanie Le Fran??ois Denis Bourque, Lucie Bourget, Denis Groleau, Bernard Massie and Carlos B. M??guez (Applied and Environmental Microbiology, Aug. 2010, p. 5058???5066 Vol.76 doi:10.1128/AEM.00413-10)
owned by: james
owned by: myers
mutable description: ￼￼￼This promoter is composed by phage T5 promoter and Cumate operator. It is repressed in the presence of CymR protein which binds the Cumate Operator.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1136_
m user id: 0
owner id: 12660
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: We added the standard iGEM prefix and suffix in order to clone it into the standard iGEM vector pSB1C3
star: false
creator: Federico Colombo, Elisa Clagnan
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_K875001_sequence/1

Tse2
type: ComponentDefinition
persistent identity: BBa K314200
display id: BBa_K314200
version: 1
was generated by: iGEM to SBOL conversion
description: Toxin Tse2
created: 2010-10-17T11:00:00Z
modified: 2015-05-08T01:11:55Z
mutable provenance: pseudomonas aeruginosa
owned by: james
owned by: myers
mutable description: A toxic protein originating from pseudomonas aeruginosa that has been shown to arrest growth in both prokaryotic and eukaryotic cells when expressed intracelluarly. It is a substrate p. aruginosas type 6 secretion system
discontinued: false
dominant: false
experience: experience/Works
group u list: _496_
m user id: 0
owner id: 6395
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: mention something about promoter needs to have no leaky expression for succesful cloning
star: false
creator: Matthew Coyne, Ingrid Swanson, Jesa Landis, Matthew Harger
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tse2
sequence: https://synbiohub.org/public/igem/BBa_K314200_sequence/1

tse2
type: SequenceAnnotation
persistent identity: annotation2089456
display id: annotation2089456
version: 1
top level: Tse2
location: https://synbiohub.org/public/igem/BBa_K314200/annotation2089456/range2089456/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K314200/annotation2089456/range2089456/1
type: Range
persistent identity: range2089456
display id: range2089456
version: 1
top level: Tse2
direction: direction/forward
start: 1
end: 477
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_K215100
type: ComponentDefinition
persistent identity: BBa K215100
display id: BBa_K215100
version: 1
was generated by: iGEM to SBOL conversion
description: prtD from erwinia chrysanthemi
created: 2009-09-29T11:00:00Z
modified: 2015-05-08T01:11:30Z
mutable provenance: Gene Bank ID: Erwinia chrysanthemi M60395.1
owned by: james
owned by: myers
mutable description: This is one of the 3 genes: prtD, prtE, prtF, that are from a type I Erwinia chrysanthemi secretion system.
discontinued: false
dominant: false
group u list: _320_
m user id: 0
owner id: 5596
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Synthesized to be bio-bricked, keeps original protein sequence.
star: false
creator: Josef Dunbar
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: native RBS
sequence annotation: prtD
sequence: https://synbiohub.org/public/igem/BBa_K215100_sequence/1

native RBS
type: SequenceAnnotation
persistent identity: annotation2027445
display id: annotation2027445
version: 1
top level: BBa_K215100
location: https://synbiohub.org/public/igem/BBa_K215100/annotation2027445/range2027445/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K215100/annotation2027445/range2027445/1
type: Range
persistent identity: range2027445
display id: range2027445
version: 1
top level: BBa_K215100
direction: direction/forward
start: 3
end: 18
orientation: inline

prtD
type: SequenceAnnotation
persistent identity: annotation2027446
display id: annotation2027446
version: 1
top level: BBa_K215100
location: https://synbiohub.org/public/igem/BBa_K215100/annotation2027446/range2027446/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K215100/annotation2027446/range2027446/1
type: Range
persistent identity: range2027446
display id: range2027446
version: 1
top level: BBa_K215100
direction: direction/forward
start: 19
end: 1749
orientation: inline

BBa_K215102
type: ComponentDefinition
persistent identity: BBa K215102
display id: BBa_K215102
version: 1
was generated by: iGEM to SBOL conversion
description: prtF from erwinia chrysanthemi
created: 2009-09-29T11:00:00Z
modified: 2015-05-08T01:11:30Z
mutable provenance: Gene Bank ID: Erwinia chrysanthemi M60395.1
owned by: myers
owned by: james
mutable description: This is one of the 3 genes: prtD, prtE, prtF, that are from a type I Erwinia chrysanthemi secretion system.
discontinued: false
dominant: false
group u list: _320_
m user id: 0
owner id: 5596
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Synthesized to be bio-bricked, keeps original protein sequence and ribosome binding site.
star: false
creator: Josef Dunbar
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Native RBS
sequence annotation: prtF
sequence: https://synbiohub.org/public/igem/BBa_K215102_sequence/1

Native RBS
type: SequenceAnnotation
persistent identity: annotation2027449
display id: annotation2027449
version: 1
top level: BBa_K215102
location: https://synbiohub.org/public/igem/BBa_K215102/annotation2027449/range2027449/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K215102/annotation2027449/range2027449/1
type: Range
persistent identity: range2027449
display id: range2027449
version: 1
top level: BBa_K215102
direction: direction/forward
start: 3
end: 14
orientation: inline

prtF
type: SequenceAnnotation
persistent identity: annotation2027450
display id: annotation2027450
version: 1
top level: BBa_K215102
location: https://synbiohub.org/public/igem/BBa_K215102/annotation2027450/range2027450/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K215102/annotation2027450/range2027450/1
type: Range
persistent identity: range2027450
display id: range2027450
version: 1
top level: BBa_K215102
direction: direction/forward
start: 15
end: 1403
orientation: inline

BBa_K215101
type: ComponentDefinition
persistent identity: BBa K215101
display id: BBa_K215101
version: 1
was generated by: iGEM to SBOL conversion
description: prtE from erwinia chrysanthemi
created: 2009-09-29T11:00:00Z
modified: 2015-05-08T01:11:30Z
mutable provenance: Gene Bank ID: Erwinia chrysanthemi M60395.1
owned by: james
owned by: myers
mutable description: This is one of the 3 genes: prtD, prtE, prtF, that are from a type I Erwinia chrysanthemi secretion system.
discontinued: false
dominant: false
group u list: _320_
m user id: 0
owner id: 5596
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Synthesized to be bio-bricked, keeps original protein sequence and rhibosome binding site.
star: false
creator: Josef Dunbar
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: native rbs
sequence annotation: prtE
sequence: https://synbiohub.org/public/igem/BBa_K215101_sequence/1

native rbs
type: SequenceAnnotation
persistent identity: annotation2027447
display id: annotation2027447
version: 1
top level: BBa_K215101
location: https://synbiohub.org/public/igem/BBa_K215101/annotation2027447/range2027447/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K215101/annotation2027447/range2027447/1
type: Range
persistent identity: range2027447
display id: range2027447
version: 1
top level: BBa_K215101
direction: direction/forward
start: 3
end: 15
orientation: inline

prtE
type: SequenceAnnotation
persistent identity: annotation2027448
display id: annotation2027448
version: 1
top level: BBa_K215101
location: https://synbiohub.org/public/igem/BBa_K215101/annotation2027448/range2027448/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K215101/annotation2027448/range2027448/1
type: Range
persistent identity: range2027448
display id: range2027448
version: 1
top level: BBa_K215101
direction: direction/forward
start: 16
end: 1362
orientation: inline

https://synbiohub.org/public/igem/BBa_K1175400_sequence/1
type: Sequence
persistent identity: BBa K1175400 sequence
display id: BBa_K1175400_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaggggagagagccctgccatgaacgcgtctagcgaacgtgaccgttctctgtttggtgtgctgcgtcagttccgccgctccttttggtctgttggcatcttcagcgcggtcatcaatgttctgatgctggcacctagcgtctacatgctccaggtttacgaccgtgtcctcgccagcggcaacggtattactctgctgatgctcacgctcctgatggcgggtctgtgcgccttcatgggtgctctcgaatgggttcgcagcctgctggtagttcgtctcggtactcgcatcgacctcgcgctcaatcaagacgtttttaacgcggctttcgcgcgtaatctggaagctggtgatggccgtgccggtctggcgctgaccgacctcaccctgctccgtcaattcatcacgggtaatgcactgttcgcgttcttcgacgttccgtggttcccgctctttctcctcgtactgttcctgctgcacccttggctcggcatgctcgcactgggtggtacggttgtacctggtggcgtaggcctcgcagaaccggcgtctgaccagtctaccgcgggtggctctaatcaacaaagccaacaagccactcatctcgcggatgcgcagctccgtaatgcggacgttatcgaagcgatgggtatgctcggtaacctccgtcgtcgctggctggcgcgtcactaccgctttatctccctccaaaatctcgcttccgagcgcgcagccgccgttggtggtgcgtccaaatactctcgtattgcactccaaagcctcatgctgggcctcggtgcgctcctcgcgatcgacggcaaaatcacgccgggtatgatgattgcgggctctatcctcgtcggtcgtgttctgtccccgattgaccagctgatcggtgtttggaaacagtggtcttctgcccgcattgcgtggcagcgcctgacccgtctgattgccgcatatccgccacgccctgcggctatggctctcccagcgccggaaggccacctgtccgttgagcaagtttctctgcgtaccgcacaaggcaatactcgcctgcaaaacatccatttctccctgcaagcgggtgagaccctggttatcctgggcgcctctggttctggcaaatcttctctcgctcgtctgctcgtgggtgcacaatcccctactcaaggtaaagttcgcctcgatggtgcggacctgaaccaagttgacaaaaacaccttcggtccgaccattggttacctcccgcaggacgttcaactgttcaaaggtagcctggcggaaaacattgcccgtttcggcgacgccgacccggaaaaggttgttgcggcagctaagctcgccggtgttcacgaactcatcctgtctctgcctaatggttacgacactgagctgggtgacggcggtggcggcctgtctggtggtcaacgtcagcgtatcggtctcgcacgcgcaatgtacggcgacccgtgtctcctcattctggacgaaccgaacgcatctctggactctgagggtgaccaagcactcatgcaggctattgtggcgctccagaaacgtggtgcaaccgttgtactcattacgcaccgtccggcactcaccaccctcgctcagaaaatcctgatcctccacgagggtcaacagcaacgtatgggcctggcccgtgacgttctgacggaactccagcagcgctccgcggcaaaccaggcgcgtatgaacccgaccgcagcaatgccacagtaataatactagagagcagggatctcgtgatgactggtatggatattaccactcaggacgaactcaacgaagctgccatgcgtgaccgtgcgagccgtgacgaagaacgtgcgctccgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K215104_sequence/1
type: Sequence
persistent identity: BBa K215104 sequence
display id: BBa_K215104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggggagagagccctgccatgaacgcgtctagcgaacgtgaccgttctctgtttggtgtgctgcgtcagttccgccgctccttttggtctgttggcatcttcagcgcggtcatcaatgttctgatgctggcacctagcgtctacatgctccaggtttacgaccgtgtcctcgccagcggcaacggtattactctgctgatgctcacgctcctgatggcgggtctgtgcgccttcatgggtgctctcgaatgggttcgcagcctgctggtagttcgtctcggtactcgcatcgacctcgcgctcaatcaagacgtttttaacgcggctttcgcgcgtaatctggaagctggtgatggccgtgccggtctggcgctgaccgacctcaccctgctccgtcaattcatcacgggtaatgcactgttcgcgttcttcgacgttccgtggttcccgctctttctcctcgtactgttcctgctgcacccttggctcggcatgctcgcactgggtggtacggttgtacctggtggcgtaggcctcgcagaaccggcgtctgaccagtctaccgcgggtggctctaatcaacaaagccaacaagccactcatctcgcggatgcgcagctccgtaatgcggacgttatcgaagcgatgggtatgctcggtaacctccgtcgtcgctggctggcgcgtcactaccgctttatctccctccaaaatctcgcttccgagcgcgcagccgccgttggtggtgcgtccaaatactctcgtattgcactccaaagcctcatgctgggcctcggtgcgctcctcgcgatcgacggcaaaatcacgccgggtatgatgattgcgggctctatcctcgtcggtcgtgttctgtccccgattgaccagctgatcggtgtttggaaacagtggtcttctgcccgcattgcgtggcagcgcctgacccgtctgattgccgcatatccgccacgccctgcggctatggctctcccagcgccggaaggccacctgtccgttgagcaagtttctctgcgtaccgcacaaggcaatactcgcctgcaaaacatccatttctccctgcaagcgggtgagaccctggttatcctgggcgcctctggttctggcaaatcttctctcgctcgtctgctcgtgggtgcacaatcccctactcaaggtaaagttcgcctcgatggtgcggacctgaaccaagttgacaaaaacaccttcggtccgaccattggttacctcccgcaggacgttcaactgttcaaaggtagcctggcggaaaacattgcccgtttcggcgacgccgacccggaaaaggttgttgcggcagctaagctcgccggtgttcacgaactcatcctgtctctgcctaatggttacgacactgagctgggtgacggcggtggcggcctgtctggtggtcaacgtcagcgtatcggtctcgcacgcgcaatgtacggcgacccgtgtctcctcattctggacgaaccgaacgcatctctggactctgagggtgaccaagcactcatgcaggctattgtggcgctccagaaacgtggtgcaaccgttgtactcattacgcaccgtccggcactcaccaccctcgctcagaaaatcctgatcctccacgagggtcaacagcaacgtatgggcctggcccgtgacgttctgacggaactccagcagcgctccgcggcaaaccaggcgcgtatgaacccgaccgcagcaatgccacagtaataatactagagagcagggatctcgtgatgactggtatggatattaccactcaggacgaactcaacgaagctgccatgcgtgaccgtgcgagccgtgacgaagaacgtgcgctccgtctgggttggtggctcgtcctcgcgggtttcggtggtttcctcctgtgggcgctcctggctccactggacaagggtgttgcggttcagggtaacgttgtagtttccggtaaccgtaaagttatccagcacatgcaaggc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0031_sequence/1
type: Sequence
persistent identity: BBa B0031 sequence
display id: BBa_B0031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K215001_sequence/1
type: Sequence
persistent identity: BBa K215001 sequence
display id: BBa_K215001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggacgttgaagcatggctgggtgcccgtgtaccgctggttgaaacgggcagcgcacatcaccaccatcatcatggttccgccgaaaacctctactttcagggcggctctgctgctagcggttctgccgagaatctgtacttccaaggtggctccgctcatcaccaccatcatcacggtagcgcaggcggcactgacacctttgacttctctggttactctaacaatcagcgtatcaacctgaacgagggttctttctctgacgttggtggtctgaagggcaacgtttctatcgcgcatggtgttaccatcgaaaacgcgatcggtggttctggtaacgacatcctgatcggtaatggcgcggataacatcctccagggtggtgcgggtgacgacgttctgtacggttctacgggtgcggacacgctgacgggtggcgctggtcgtgacattttcgtatatggtagcggtcaagactctaccgtttctgcatacgactggatcacggattttcagacgggtatcgacaaaatcgacctgtccgctttccgtaatgaaggtcagctgtcttttgttcaggaccagtttactggtaaaggccaggaggttatgctgcaatgggacgcggcgaactctacgaccaatctgtggctgcacgaagcgggtcactcttctgttgactttctcgttcgtatcgttggccagaccgcgcagagcgacattatcgtctaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K875001_sequence/1
type: Sequence
persistent identity: BBa K875001 sequence
display id: BBa_K875001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaacaaacagacaatctggtctgtttgtattat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K875004_sequence/1
type: Sequence
persistent identity: BBa K875004 sequence
display id: BBa_K875004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagaaagaggagaaatactaatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggagatattctgctgacccagagcccggcgattctgagcgtgagcccgggcgaacgcgtgagctttagctgccgcgcgagccagagcattggcgtgctgtgcgatcgcattagcattcattggtatcagcagcgcaccaacggcagcccgcgcctgctgattaaatatgcgagcgaaagcattagcggcattccgagccgctttagcggcagcggcagcggcaccgattttaccctgagcattaacagcgtggaaagcgaagatattgcggattattattgccagcagagcaacagctggccgtggacctttggcggcggcaccaaactggaaattaaaggcggcaaaggcagcggcggcaaaggcaccggcggcaaaggcagcggcggcaaaggcagcgaagtgaaactggtggaaagcggcggcgatctggtgcagccgggcggcagcctgaaactgagctgcgcggtgcattgcgatcgcaccagcggctttacctttagcgattattatatgtattgggtgcgccagaccccggaaaaacgcctggaatgggtggcgtatattagcagcgtgcattgcgatcgcggcggcggcagcaccttttatccagataccgtgaaaggccgctttaccattagccgcgataacgcgaaaaacaccctgtatctacagatgagccgcctgaaaagcgatgataccgcgatgtattattgcgcgcgcgcgtatcatggcaactattttgattattggggccagggcaccaccctgaccgtgagcagctccggtcaccaccaccaccaccactgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K314200_sequence/1
type: Sequence
persistent identity: BBa K314200 sequence
display id: BBa_K314200_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtcctacgactacgagaaaaccagcctcaccctctaccgggcggtattcaaggccaactacgacggcgacgtcggtcgctacctgcatcccgacaaggaactcgccgaggctgcggaagtcgccccgctgctgcatccgaccttcgacagccccaacacccctggcgtccccgcccgcgcgccggacatcgtcgccggccgcgacggcctctacgccccggacaccggcggcacctcggtgttcgaccgcgccggcgtgctgcgccgcgccgacggcgacttcgtgatacccgacggcaccgacatcccgccggaccttaaggtgaagcaggacagctacaacaagcgcctgcaagccacccactacaccatcatgccggccaagccgatgtaccgggaggtcctcatgggccaactggacaacttcgtgcgcaacgccatccgccgccaatgggaaaaagcccgcgggctctag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K206000_sequence/1
type: Sequence
persistent identity: BBa K206000 sequence
display id: BBa_K206000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K215100_sequence/1
type: Sequence
persistent identity: BBa K215100 sequence
display id: BBa_K215100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggggagagagccctgccatgaacgcgtctagcgaacgtgaccgttctctgtttggtgtgctgcgtcagttccgccgctccttttggtctgttggcatcttcagcgcggtcatcaatgttctgatgctggcacctagcgtctacatgctccaggtttacgaccgtgtcctcgccagcggcaacggtattactctgctgatgctcacgctcctgatggcgggtctgtgcgccttcatgggtgctctcgaatgggttcgcagcctgctggtagttcgtctcggtactcgcatcgacctcgcgctcaatcaagacgtttttaacgcggctttcgcgcgtaatctggaagctggtgatggccgtgccggtctggcgctgaccgacctcaccctgctccgtcaattcatcacgggtaatgcactgttcgcgttcttcgacgttccgtggttcccgctctttctcctcgtactgttcctgctgcacccttggctcggcatgctcgcactgggtggtacggttgtacctggtggcgtaggcctcgcagaaccggcgtctgaccagtctaccgcgggtggctctaatcaacaaagccaacaagccactcatctcgcggatgcgcagctccgtaatgcggacgttatcgaagcgatgggtatgctcggtaacctccgtcgtcgctggctggcgcgtcactaccgctttatctccctccaaaatctcgcttccgagcgcgcagccgccgttggtggtgcgtccaaatactctcgtattgcactccaaagcctcatgctgggcctcggtgcgctcctcgcgatcgacggcaaaatcacgccgggtatgatgattgcgggctctatcctcgtcggtcgtgttctgtccccgattgaccagctgatcggtgtttggaaacagtggtcttctgcccgcattgcgtggcagcgcctgacccgtctgattgccgcatatccgccacgccctgcggctatggctctcccagcgccggaaggccacctgtccgttgagcaagtttctctgcgtaccgcacaaggcaatactcgcctgcaaaacatccatttctccctgcaagcgggtgagaccctggttatcctgggcgcctctggttctggcaaatcttctctcgctcgtctgctcgtgggtgcacaatcccctactcaaggtaaagttcgcctcgatggtgcggacctgaaccaagttgacaaaaacaccttcggtccgaccattggttacctcccgcaggacgttcaactgttcaaaggtagcctggcggaaaacattgcccgtttcggcgacgccgacccggaaaaggttgttgcggcagctaagctcgccggtgttcacgaactcatcctgtctctgcctaatggttacgacactgagctgggtgacggcggtggcggcctgtctggtggtcaacgtcagcgtatcggtctcgcacgcgcaatgtacggcgacccgtgtctcctcattctggacgaaccgaacgcatctctggactctgagggtgaccaagcactcatgcaggctattgtggcgctccagaaacgtggtgcaaccgttgtactcattacgcaccgtccggcactcaccaccctcgctcagaaaatcctgatcctccacgagggtcaacagcaacgtatgggcctggcccgtgacgttctgacggaactccagcagcgctccgcggcaaaccaggcgcgtatgaacccgaccgcagcaatgccacagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K215101_sequence/1
type: Sequence
persistent identity: BBa K215101 sequence
display id: BBa_K215101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agcagggatctcgtgatgactggtatggatattaccactcaggacgaactcaacgaagctgccatgcgtgaccgtgcgagccgtgacgaagaacgtgcgctccgtctgggttggtggctcgtcctcgcgggtttcggtggtttcctcctgtgggcgctcctggctccactggacaagggtgttgcggttcagggtaacgttgtagtttccggtaaccgtaaagttatccagcacatgcaaggcggtattgttgaccgcatccaagttaaggacggcgaccgtgttgccgctggtcaggttctgctcactctcaatgcagtcgacgctcgtaccacctccgagggcctgggttctcagtacgatcagctcatcgcgcgtgaagcgcgcctcctcgccgagcagcgtaaccagtcttctctcgctgcaacgccacgcctgacgcaggcacgccagcgtccggaaatggcggcgattatcgccctccaggaagacctgctgcgctctcgtcagcagtctctcaaactggaaattgacggtgtccgtgcatctatcgatggtctggaaacttctctgggtgctctgcaaaaagtgatgtcttctaaacagtctgaacaagcgactctgtctcaacagctgcaaggtctgcgtccgctggccgcagacaactacgttccgcgtaacaagatgctcgaaaccgaacgtctcttcgcccaggtttctggcgagctggcccagacctctggtgaagttggtcgtacccgtcgtgacattcaacaacagaaactccgcattgcgcagcgccagcaggagtacgacaaagaagtcaactctgaactgtctgacgttcaggccaaactgaacgaagtaatctctcagcgtgaaaaggcggacttcaatctcgctaacgtgcaggttcgtgcgccggtggcgggcactgtggtggatatgaaaatcttcaccgaaggtggcgtaattgctccgggtcaagttatgatggacattgttccggaggatcagccactgctggtagatggccgtatcccggttgagatggttgacaaagtatggagcggtctgccggtcgaactgcaatttactgcgttttcccaatctactacgccgcgtgtccctggtactgtaaccctgctgagcgcggatcgcctggtggacgaaaaagatggcaccccttactatggcctccgtattcaggtgtctgaggaaggtaaacgtagcctccacggcctggagattaaaccgggtatgcctgtccaaggttttgtgcgtacgggcgaacgctctttcatcaactacctcttcaaaccgctcatggaccgtatgcacctcgcactgaccgaagaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K215102_sequence/1
type: Sequence
persistent identity: BBa K215102 sequence
display id: BBa_K215102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agcggaaggaagcaatgcgccgtaaagcagttctgctcactgtggttctgagcctgtctggtggtagcgcccaagcgatgggtctgctggacgcgtgggaactcgcgctccgcaatgatgcgcaactgcgtgcggcaggtttcgaacgtgacgcgggtcaagaggaagttgcgattggtcgcgcaggcctcctgccgagcctgcaatacacgtacggtgcgaactactcccactccaaagttacccagcgcgatcgtaccctgaacaacaccacgaaacgtgattatgataactacgtttctacgctgaccctgcgtcagccactcctggactacgcggcatgggcgcgttatcagcaaggcgtaactcgtaaactgatggcggaccagcgttttcgcgaccgttcccaggatctgatggttcgtctgtaccagtcctggagcgaagccctgctggcgcaggaaaagctgatgctcctcgacgctcagcgtcgtgcataccaggaacagctcgcactgaatcgccgtctcctggctgccggtgagggcacgcaaaccgacctgcgtgagaccgaagcacgttataccgtcaccgaggcgcagcgtatcgaacaggaggacaccctcgatgccgccatgactgacctggaaaacatgatgggctctcctctccagatccaagacctgtctccactggctctcgacactctcccggataacgtaacggaaaaccgtagcctctctcagtggcgtgaactcacggtacgtcacaacgccaaactggcagtacaacgtgagaacgtggattactctcgctacgaaatcgagcgcaaccgtgcgggccacctcccgaccctggacctggttgcctctacccgtaattccctctccgagagcgaatacaactacaatcaaaaatacgacacccagaccgtaggtctgcaagttcgtgttcctctctactctggcggtgcggtttctgcgtctatgcgccaagcggcagctgagtatcaacagtcccaggcggaactggacaatcagactcgtcagaccttcgcggagctgcgtcgccagttcaacctgtgcgcaaacggtgctgcgaaaatccgtgcttggcagatgtctgtagctgctgccgaagaggccattcgtgcgacccgtcagtctgttgcgggtggtgaacgtattaacctggatgtgctcatggctgagcaggaatggtataatgcccgtcgtgagctgacggaggttaagtaccgttggctgcaagcgtggctcaatctgcgctacacggctggtactctcaacgaacaagatatgatgcagctggcagcctggttccagtctgcgccggttatcaataaaaccggcatcaatgctgccacgggcaacaagaccaattaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z