BBa_K1176888 1 BBa_K1176888 Mtd (BPP-1 bacteriophage) 2013-08-28T11:00:00Z 2015-05-08T01:09:35Z This part is naturally occurring in the genomic DNA of the Bordetella BPP-1 bacteriophage. Citation: Overstreet, C. M., Yuan, T. Z., Levin, A. M., Kong, C., Coroneus, J. G., & Weiss, G. a. (2012). Self-made phage libraries with heterologous inserts in the Mtd of Bordetella bronchiseptica. Protein engineering, design & selection : PEDS, 25(4), 145???51. doi:10.1093/protein/gzr068 This part codes for the Major Tropism Determinant (mtd) protein responsible for selective protein binding to the surface markers on Bordetella bacteria. It has several specific nucleotides in its sequence that can be mutagenized to change the binding properties of the protein without adversely affecting the folding and stability of the protein. false false _1489_ 0 18032 9 It's complicated false In designing this part, we had to alter 3 restriction sites naturally occurring in the sequence: PstI, EcoRI, and NotI. false Michael Cheng BBa_K1176888_sequence 1 atgagtaccgcagtccagttccgcggcggcacgaccgcccagcacgccacgttcacgggcgccgcccgtgagattaccgtcgataccgacaagaacacggtcgttgtgcatgacggtgccaccgctggcggcttccccctggcgcggcacgatctggtgaaaacggctttcatcaaggccgacaagtcggccgtcgccttcacgcgcaccggcaacgcaacggccagcatcaaggctggcaccatcgtggaggtcaacggcaagctggtgcagttcaccgccgacacggccatcaccatgccggcgctgacggccggcaccgactacgccatctacgtctgcgacgatggcacggtgcgcgccgattccaacttttcggcgcccactggctacacctcgaccacggcgcgcaaggtgggcggcttccactatgcgccgggaagcaacgcggcagcgcaggctggcggaaacaccacggcgcagatcaacgaatacagcctgtgggacatcaagttccgcccggctgcgctcgacccgcgcggcatgacgctggtggccggcgcgttttgggcggacatctatctgctaggcgtcaaccacctgaccgatggcaccagcaaatacaacgtgaccatcgcggacggcagcgcgtccccgaagaaatctaccaagttcggcggcgacggcagcgcggcctacagcgacggcgcctggtacaacttcgccgaggtcatgactcaccacggcaagcgcctgcccaactacaacgagttccaggcgctggctttcggcacgaccgaggctacgtccagcggcggcaccgacgtgcccaccaccggcgtgaacggcacgggcgccacgagcgcgtggaacatcttcacgtccaagtggggcgttgtgcaggcgtccggttgcttgtggacgtggggtaacgagttcggcggcgtgaatggcgcatccgaatacacggccaacactggaggccgcggatcggtgtacgcccagcccgctgctgcgctattcggcggcgcctggaacggcacgtcgctctcgggttctcgcgctgcgctctggtacagcgggccgtcgttctcgttcgcgttcttcggggcgcgcggcgtctgtgaccacctgattcttgag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z