LOCUS BBa_K1179002 5141 bp DNA linear 14-Nov-2024 DEFINITION Hef1A_Cas9-VP16 ACCESSION BBa_K1179002 VERSION BBa_K1179002.1 FEATURES Location/Qualifiers promoter 1..1174 /label=Hef1A promoter 1..1174 /label=BBa_K779200 misc_feature 1176..1196 /label=attB1 misc_feature 1175..1196 /label=BBa_J85600 CDS 1197..5141 /label=BBa_K1179009 ORIGIN 1 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgt 61 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 121 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 181 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 241 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 301 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 361 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 421 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 481 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 541 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 601 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 661 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 721 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 781 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 841 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 901 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 961 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 1021 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 1081 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 1141 cagtggttca aagttttttt cttccatttc aggtccaagt ttgtacaaaa aagcagatga 1201 tcaaatttcg gggacacttc ctcatcgagg gggacctgaa cccagacaac agcgatgtcg 1261 acaaactctt tatccaactg gttcagactt acaatcagct tttcgaagag aacccgatca 1321 acgcatccgg agttgacgcc aaagcaatcc tgagcgctag gctgtccaaa tcccggcggc 1381 tcgaaaacct catcgcacag ctccctgggg agaagaagaa cggcctgttt ggtaatctta 1441 tcgccctgtc actcgggctg acccccaact ttaaatctaa cttcgacctg gccgaagatg 1501 ccaagcttca actgagcaaa gacacctacg atgatgatct cgacaatctg ctggcccaga 1561 tcggcgacca gtacgcagac ctttttttgg cggcaaagaa cctgtcagac gccattctgc 1621 tgagtgatat tctgcgagtg aacacggaga tcaccaaagc tccgctgagc gctagtatga 1681 tcaagcgcta tgatgagcac caccaagact tgactttgct gaaggccctt gtcagacagc 1741 aactgcctga gaagtacaag gaaattttct tcgatcagtc taaaaatggc tacgccggat 1801 acattgacgg cggagcaagc caggaggaat tttacaaatt tattaagccc atcttggaaa 1861 aaatggacgg caccgaggag ctgctggtaa agcttaacag agaagatctg ttgcgcaaac 1921 agcgcacttt cgacaatgga agcatccccc accagattca cctgggcgaa ctgcacgcta 1981 tcctcaggcg gcaagaggat ttctacccct ttttgaaaga taacagggaa aagattgaga 2041 aaatcctcac atttcggata ccctactatg taggccccct cgcccgggga aattccagat 2101 tcgcgtggat gactcgcaaa tcagaagaga caatcactcc ctggaacttc gaggaagtcg 2161 tggataaggg ggcctctgcc cagtccttca tcgaaaggat gactaacttt gataaaaatc 2221 tgcctaacga aaaggtgctt cctaaacact ctctgctgta cgagtacttc acagtttata 2281 acgagctcac caaggtcaaa tacgtcacag aagggatgag aaagccagca ttcctgtctg 2341 gagagcagaa gaaagctatc gtggacctcc tcttcaagac gaaccggaaa gttaccgtga 2401 aacagctcaa agaagactat ttcaaaaaga ttgaatgttt cgactctgtt gaaatcagcg 2461 gagtggagga tcgcttcaac gcatccctgg gaacgtatca cgatctcctg aaaatcatta 2521 aagacaagga cttcctggac aatgaggaga acgaggacat tcttgaggac attgtcctca 2581 cccttacgtt gtttgaagat agggagatga ttgaagaacg cttgaaaact tacgctcatc 2641 tcttcgacga caaagtcatg aaacagctca agaggcgccg atatacagga tgggggcggc 2701 tgtcaagaaa actgatcaat gggatccgag acaagcagag tggaaagaca atcctggatt 2761 ttcttaagtc cgatggattt gccaaccgga acttcatgca gttgatccat gatgactctc 2821 tcacctttaa ggaggacatc cagaaagcac aagtttctgg ccagggggac agtcttcacg 2881 agcacatcgc taatcttgca ggtagcccag ctatcaaaaa gggaatactg cagaccgtta 2941 aggtcgtgga tgaactcgtc aaagtaatgg gaaggcataa gcccgagaat atcgttatcg 3001 agatggcccg agagaaccaa actacccaga agggacagaa gaacagtagg gaaaggatga 3061 agaggattga agagggtata aaagaactgg ggtcccaaat ccttaaggaa cacccagttg 3121 aaaacaccca gcttcagaat gagaagctct acctgtacta cctgcagaac ggcagggaca 3181 tgtacgtgga tcaggaactg gacatcaatc ggctctccga ctacgacgtg gatgccatcg 3241 tgccccagtc ttttctcaaa gatgattcta ttgataataa agtgttgaca agatccgata 3301 aaaatagagg gaagagtgat aacgtcccct cagaagaagt tgtcaagaaa atgaaaaatt 3361 attggcggca gctgctgaac gccaaactga tcacacaacg gaagttcgat aatctgacta 3421 aggctgaacg aggtggcctg tctgagttgg ataaagccgg cttcatcaaa aggcagcttg 3481 ttgagacacg ccagatcacc aagcacgtgg cccaaattct cgattcacgc atgaacacca 3541 agtacgatga aaatgacaaa ctgattcgag aggtgaaagt tattactctg aagtctaagc 3601 tggtgtcaga tttcagaaag gactttcagt tttataaggt gagagagatc aacaattacc 3661 accatgcgca tgatgcctac ctgaatgcag tggtaggcac tgcacttatc aaaaaatatc 3721 ccaagcttga atctgaattt gtttacggag actataaagt gtacgatgtt aggaaaatga 3781 tcgcaaagtc tgagcaggaa ataggcaagg ccaccgctaa gtacttcttt tacagcaata 3841 ttatgaattt tttcaagacc gagattacac tggccaatgg agagattcgg aagcgaccac 3901 ttatcgaaac aaacggagaa acaggagaaa tcgtgtggga caagggtagg gatttcgcga 3961 cagtccggaa ggtcctgtcc atgccgcagg tgaacatcgt taaaaagacc gaagtacaga 4021 ccggaggctt ctccaaggaa agtatcctcc cgaaaaggaa cagcgacaag ctgatcgcac 4081 gcaaaaaaga ttgggacccc aagaaatacg gcggattcga ttctcctaca gtcgcttaca 4141 gtgtactggt tgtggccaaa gtggagaaag ggaagtctaa aaaactcaaa agcgtcaagg 4201 aactgctggg catcacaatc atggagcgat caagcttcga aaaaaacccc atcgactttc 4261 tcgaggcgaa aggatataaa gaggtcaaaa aagacctcat cattaagctt cccaagtact 4321 ctctctttga gcttgaaaac ggccggaaac gaatgctcgc tagtgcgggc gagctgcaga 4381 aaggtaacga gctggcactg ccctctaaat acgttaattt cttgtatctg gccagccact 4441 atgaaaagct caaagggtcc cccgaagata atgagcagaa gcagctgttc gtggaacaac 4501 acaaacacta ccttgatgag atcatcgagc aaataagcga attctccaaa agagtgatcc 4561 tcgccgacgc taacctcgat aaggtgcttt ctgcttacaa taagcacagg gataagccca 4621 tcagggagca ggcagaaaac attatccact tgtttactct gaccaacttg ggcgcgcctg 4681 cagccttcaa gtacttcgac accaccatag acagaaagcg gtacacctct acaaaggagg 4741 tcctggacgc cacactgatt catcagtcaa ttacggggct ctatgaaaca agaatcgacc 4801 tctctcagct cggtggagac agcagggctg accccaagaa gaagaggaag gtgtgtggtg 4861 gttctggtgg tggttctggt atgacccccc cgaccgatgt cagcctgggg gacgagctcc 4921 acttagacgg cgaggacgtg gcgatggcgc atgccgacgc gctagacgat ttcgatctgg 4981 acatgttggg ggacggggat tccccgggtc cgggatttac cccccacgac tccgccccct 5041 acggcgctct ggatatggcc gacttcgagt ttgagcagat gtttaccgat gcccttggaa 5101 ttgacgagta cggtgggacg cgtgagcttc agtgcaggtg a //