LOCUS BBa_K1179008 4255 bp DNA linear 25-Nov-2024 DEFINITION A mutant DNA binding Cas9 with a C-terminal linker and BsaI site for ligation ACCESSION BBa_K1179008 VERSION BBa_K1179008.1 FEATURES Location/Qualifiers ORIGIN 1 ggatcccggt accctcgaca cctgctgaca ggtcccttgc caccatggac aagaagtact 61 ccattgggct cgctatcggc acaaacagcg tcggctgggc cgtcattacg gacgagtaca 121 aggtgccgag caaaaaattc aaagttctgg gcaataccga tcgccacagc ataaagaaga 181 acctcattgg cgccctcctg ttcgactccg gggagacggc cgaagccacg cggctcaaaa 241 gaacagcacg gcgcagatat acccgcagaa agaatcggat ctgctacctg caggagatct 301 ttagtaatga gatggctaag gtggatgact ctttcttcca taggctggag gagtcctttt 361 tggtggagga ggataaaaag cacgagcgcc acccaatctt tggcaatatc gtggacgagg 421 tggcgtacca tgaaaagtac ccaaccatat atcatctgag gaagaagctt gtagacagta 481 ctgataaggc tgacttgcgg ttgatctatc tcgcgctggc gcatatgatc aaatttcggg 541 gacacttcct catcgagggg gacctgaacc cagacaacag cgatgtcgac aaactcttta 601 tccaactggt tcagacttac aatcagcttt tcgaagagaa cccgatcaac gcatccggag 661 ttgacgccaa agcaatcctg agcgctaggc tgtccaaatc ccggcggctc gaaaacctca 721 tcgcacagct ccctggggag aagaagaacg gcctgtttgg taatcttatc gccctgtcac 781 tcgggctgac ccccaacttt aaatctaact tcgacctggc cgaagatgcc aagcttcaac 841 tgagcaaaga cacctacgat gatgatctcg acaatctgct ggcccagatc ggcgaccagt 901 acgcagacct ttttttggcg gcaaagaacc tgtcagacgc cattctgctg agtgatattc 961 tgcgagtgaa cacggagatc accaaagctc cgctgagcgc tagtatgatc aagcgctatg 1021 atgagcacca ccaagacttg actttgctga aggcccttgt cagacagcaa ctgcctgaga 1081 agtacaagga aattttcttc gatcagtcta aaaatggcta cgccggatac attgacggcg 1141 gagcaagcca ggaggaattt tacaaattta ttaagcccat cttggaaaaa atggacggca 1201 ccgaggagct gctggtaaag cttaacagag aagatctgtt gcgcaaacag cgcactttcg 1261 acaatggaag catcccccac cagattcacc tgggcgaact gcacgctatc ctcaggcggc 1321 aagaggattt ctaccccttt ttgaaagata acagggaaaa gattgagaaa atcctcacat 1381 ttcggatacc ctactatgta ggccccctcg cccggggaaa ttccagattc gcgtggatga 1441 ctcgcaaatc agaagagaca atcactccct ggaacttcga ggaagtcgtg gataaggggg 1501 cctctgccca gtccttcatc gaaaggatga ctaactttga taaaaatctg cctaacgaaa 1561 aggtgcttcc taaacactct ctgctgtacg agtacttcac agtttataac gagctcacca 1621 aggtcaaata cgtcacagaa gggatgagaa agccagcatt cctgtctgga gagcagaaga 1681 aagctatcgt ggacctcctc ttcaagacga accggaaagt taccgtgaaa cagctcaaag 1741 aagactattt caaaaagatt gaatgtttcg actctgttga aatcagcgga gtggaggatc 1801 gcttcaacgc atccctggga acgtatcacg atctcctgaa aatcattaaa gacaaggact 1861 tcctggacaa tgaggagaac gaggacattc ttgaggacat tgtcctcacc cttacgttgt 1921 ttgaagatag ggagatgatt gaagaacgct tgaaaactta cgctcatctc ttcgacgaca 1981 aagtcatgaa acagctcaag aggcgccgat atacaggatg ggggcggctg tcaagaaaac 2041 tgatcaatgg gatccgagac aagcagagtg gaaagacaat cctggatttt cttaagtccg 2101 atggatttgc caaccggaac ttcatgcagt tgatccatga tgactctctc acctttaagg 2161 aggacatcca gaaagcacaa gtttctggcc agggggacag tcttcacgag cacatcgcta 2221 atcttgcagg tagcccagct atcaaaaagg gaatactgca gaccgttaag gtcgtggatg 2281 aactcgtcaa agtaatggga aggcataagc ccgagaatat cgttatcgag atggcccgag 2341 agaaccaaac tacccagaag ggacagaaga acagtaggga aaggatgaag aggattgaag 2401 agggtataaa agaactgggg tcccaaatcc ttaaggaaca cccagttgaa aacacccagc 2461 ttcagaatga gaagctctac ctgtactacc tgcagaacgg cagggacatg tacgtggatc 2521 aggaactgga catcaatcgg ctctccgact acgacgtgga tgccatcgtg ccccagtctt 2581 ttctcaaaga tgattctatt gataataaag tgttgacaag atccgataaa aatagaggga 2641 agagtgataa cgtcccctca gaagaagttg tcaagaaaat gaaaaattat tggcggcagc 2701 tgctgaacgc caaactgatc acacaacgga agttcgataa tctgactaag gctgaacgag 2761 gtggcctgtc tgagttggat aaagccggct tcatcaaaag gcagcttgtt gagacacgcc 2821 agatcaccaa gcacgtggcc caaattctcg attcacgcat gaacaccaag tacgatgaaa 2881 atgacaaact gattcgagag gtgaaagtta ttactctgaa gtctaagctg gtgtcagatt 2941 tcagaaagga ctttcagttt tataaggtga gagagatcaa caattaccac catgcgcatg 3001 atgcctacct gaatgcagtg gtaggcactg cacttatcaa aaaatatccc aagcttgaat 3061 ctgaatttgt ttacggagac tataaagtgt acgatgttag gaaaatgatc gcaaagtctg 3121 agcaggaaat aggcaaggcc accgctaagt acttctttta cagcaatatt atgaattttt 3181 tcaagaccga gattacactg gccaatggag agattcggaa gcgaccactt atcgaaacaa 3241 acggagaaac aggagaaatc gtgtgggaca agggtaggga tttcgcgaca gtccggaagg 3301 tcctgtccat gccgcaggtg aacatcgtta aaaagaccga agtacagacc ggaggcttct 3361 ccaaggaaag tatcctcccg aaaaggaaca gcgacaagct gatcgcacgc aaaaaagatt 3421 gggaccccaa gaaatacggc ggattcgatt ctcctacagt cgcttacagt gtactggttg 3481 tggccaaagt ggagaaaggg aagtctaaaa aactcaaaag cgtcaaggaa ctgctgggca 3541 tcacaatcat ggagcgatca agcttcgaaa aaaaccccat cgactttctc gaggcgaaag 3601 gatataaaga ggtcaaaaaa gacctcatca ttaagcttcc caagtactct ctctttgagc 3661 ttgaaaacgg ccggaaacga atgctcgcta gtgcgggcga gctgcagaaa ggtaacgagc 3721 tggcactgcc ctctaaatac gttaatttct tgtatctggc cagccactat gaaaagctca 3781 aagggtcccc cgaagataat gagcagaagc agctgttcgt ggaacaacac aaacactacc 3841 ttgatgagat catcgagcaa ataagcgaat tctccaaaag agtgatcctc gccgacgcta 3901 acctcgataa ggtgctttct gcttacaata agcacaggga taagcccatc agggagcagg 3961 cagaaaacat tatccacttg tttactctga ccaacttggg cgcgcctgca gccttcaagt 4021 acttcgacac caccatagac agaaagcggt acacctctac aaaggaggtc ctggacgcca 4081 cactgattca tcagtcaatt acggggctct atgaaacaag aatcgacctc tctcagctcg 4141 gtggagacag cagggctgac cccaagaaga agaggaaggt gtgtggtggt tctggtggtg 4201 gttctggttg agacctttgg tctcttgagc ttcagtgcag gtgaattcta ccacc //