LOCUS BBa_K1179009 3945 bp DNA linear 30-Dec-2025 DEFINITION (Cas9-VP16) A non-nuclease Cas9 with a C-terminal VP16 fusion for activation ACCESSION BBa_K1179009 VERSION BBa_K1179009.1 FEATURES Location/Qualifiers ORIGIN 1 atgatcaaat ttcggggaca cttcctcatc gagggggacc tgaacccaga caacagcgat 61 gtcgacaaac tctttatcca actggttcag acttacaatc agcttttcga agagaacccg 121 atcaacgcat ccggagttga cgccaaagca atcctgagcg ctaggctgtc caaatcccgg 181 cggctcgaaa acctcatcgc acagctccct ggggagaaga agaacggcct gtttggtaat 241 cttatcgccc tgtcactcgg gctgaccccc aactttaaat ctaacttcga cctggccgaa 301 gatgccaagc ttcaactgag caaagacacc tacgatgatg atctcgacaa tctgctggcc 361 cagatcggcg accagtacgc agaccttttt ttggcggcaa agaacctgtc agacgccatt 421 ctgctgagtg atattctgcg agtgaacacg gagatcacca aagctccgct gagcgctagt 481 atgatcaagc gctatgatga gcaccaccaa gacttgactt tgctgaaggc ccttgtcaga 541 cagcaactgc ctgagaagta caaggaaatt ttcttcgatc agtctaaaaa tggctacgcc 601 ggatacattg acggcggagc aagccaggag gaattttaca aatttattaa gcccatcttg 661 gaaaaaatgg acggcaccga ggagctgctg gtaaagctta acagagaaga tctgttgcgc 721 aaacagcgca ctttcgacaa tggaagcatc ccccaccaga ttcacctggg cgaactgcac 781 gctatcctca ggcggcaaga ggatttctac ccctttttga aagataacag ggaaaagatt 841 gagaaaatcc tcacatttcg gataccctac tatgtaggcc ccctcgcccg gggaaattcc 901 agattcgcgt ggatgactcg caaatcagaa gagacaatca ctccctggaa cttcgaggaa 961 gtcgtggata agggggcctc tgcccagtcc ttcatcgaaa ggatgactaa ctttgataaa 1021 aatctgccta acgaaaaggt gcttcctaaa cactctctgc tgtacgagta cttcacagtt 1081 tataacgagc tcaccaaggt caaatacgtc acagaaggga tgagaaagcc agcattcctg 1141 tctggagagc agaagaaagc tatcgtggac ctcctcttca agacgaaccg gaaagttacc 1201 gtgaaacagc tcaaagaaga ctatttcaaa aagattgaat gtttcgactc tgttgaaatc 1261 agcggagtgg aggatcgctt caacgcatcc ctgggaacgt atcacgatct cctgaaaatc 1321 attaaagaca aggacttcct ggacaatgag gagaacgagg acattcttga ggacattgtc 1381 ctcaccctta cgttgtttga agatagggag atgattgaag aacgcttgaa aacttacgct 1441 catctcttcg acgacaaagt catgaaacag ctcaagaggc gccgatatac aggatggggg 1501 cggctgtcaa gaaaactgat caatgggatc cgagacaagc agagtggaaa gacaatcctg 1561 gattttctta agtccgatgg atttgccaac cggaacttca tgcagttgat ccatgatgac 1621 tctctcacct ttaaggagga catccagaaa gcacaagttt ctggccaggg ggacagtctt 1681 cacgagcaca tcgctaatct tgcaggtagc ccagctatca aaaagggaat actgcagacc 1741 gttaaggtcg tggatgaact cgtcaaagta atgggaaggc ataagcccga gaatatcgtt 1801 atcgagatgg cccgagagaa ccaaactacc cagaagggac agaagaacag tagggaaagg 1861 atgaagagga ttgaagaggg tataaaagaa ctggggtccc aaatccttaa ggaacaccca 1921 gttgaaaaca cccagcttca gaatgagaag ctctacctgt actacctgca gaacggcagg 1981 gacatgtacg tggatcagga actggacatc aatcggctct ccgactacga cgtggatgcc 2041 atcgtgcccc agtcttttct caaagatgat tctattgata ataaagtgtt gacaagatcc 2101 gataaaaata gagggaagag tgataacgtc ccctcagaag aagttgtcaa gaaaatgaaa 2161 aattattggc ggcagctgct gaacgccaaa ctgatcacac aacggaagtt cgataatctg 2221 actaaggctg aacgaggtgg cctgtctgag ttggataaag ccggcttcat caaaaggcag 2281 cttgttgaga cacgccagat caccaagcac gtggcccaaa ttctcgattc acgcatgaac 2341 accaagtacg atgaaaatga caaactgatt cgagaggtga aagttattac tctgaagtct 2401 aagctggtgt cagatttcag aaaggacttt cagttttata aggtgagaga gatcaacaat 2461 taccaccatg cgcatgatgc ctacctgaat gcagtggtag gcactgcact tatcaaaaaa 2521 tatcccaagc ttgaatctga atttgtttac ggagactata aagtgtacga tgttaggaaa 2581 atgatcgcaa agtctgagca ggaaataggc aaggccaccg ctaagtactt cttttacagc 2641 aatattatga attttttcaa gaccgagatt acactggcca atggagagat tcggaagcga 2701 ccacttatcg aaacaaacgg agaaacagga gaaatcgtgt gggacaaggg tagggatttc 2761 gcgacagtcc ggaaggtcct gtccatgccg caggtgaaca tcgttaaaaa gaccgaagta 2821 cagaccggag gcttctccaa ggaaagtatc ctcccgaaaa ggaacagcga caagctgatc 2881 gcacgcaaaa aagattggga ccccaagaaa tacggcggat tcgattctcc tacagtcgct 2941 tacagtgtac tggttgtggc caaagtggag aaagggaagt ctaaaaaact caaaagcgtc 3001 aaggaactgc tgggcatcac aatcatggag cgatcaagct tcgaaaaaaa ccccatcgac 3061 tttctcgagg cgaaaggata taaagaggtc aaaaaagacc tcatcattaa gcttcccaag 3121 tactctctct ttgagcttga aaacggccgg aaacgaatgc tcgctagtgc gggcgagctg 3181 cagaaaggta acgagctggc actgccctct aaatacgtta atttcttgta tctggccagc 3241 cactatgaaa agctcaaagg gtcccccgaa gataatgagc agaagcagct gttcgtggaa 3301 caacacaaac actaccttga tgagatcatc gagcaaataa gcgaattctc caaaagagtg 3361 atcctcgccg acgctaacct cgataaggtg ctttctgctt acaataagca cagggataag 3421 cccatcaggg agcaggcaga aaacattatc cacttgttta ctctgaccaa cttgggcgcg 3481 cctgcagcct tcaagtactt cgacaccacc atagacagaa agcggtacac ctctacaaag 3541 gaggtcctgg acgccacact gattcatcag tcaattacgg ggctctatga aacaagaatc 3601 gacctctctc agctcggtgg agacagcagg gctgacccca agaagaagag gaaggtgtgt 3661 ggtggttctg gtggtggttc tggtatgacc cccccgaccg atgtcagcct gggggacgag 3721 ctccacttag acggcgagga cgtggcgatg gcgcatgccg acgcgctaga cgatttcgat 3781 ctggacatgt tgggggacgg ggattccccg ggtccgggat ttacccccca cgactccgcc 3841 ccctacggcg ctctggatat ggccgacttc gagtttgagc agatgtttac cgatgccctt 3901 ggaattgacg agtacggtgg gacgcgtgag cttcagtgca ggtga //