LOCUS BBa_K1179017 4211 bp DNA linear 22-Dec-2024 DEFINITION A mutant DNA binding Cas9 with an N-terminal linker and BsaI site for ligation ACCESSION BBa_K1179017 VERSION BBa_K1179017.1 FEATURES Location/Qualifiers ORIGIN 1 atggacaaga agtactccat tgggctcgct atcggcacaa acagcgtcgg ctgggccgtc 61 attacggacg agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa taccgatcgc 121 cacagcataa agaagaacct cattggcgcc ctcctgttcg actccgggga gacggccgaa 181 gccacgcggc tcaaaagaac agcacggcgc agatataccc gcagaaagaa tcggatctgc 241 tacctgcagg agatctttag taatgagatg gctaaggtgg atgactcttt cttccatagg 301 ctggaggagt cctttttggt ggaggaggat aaaaagcacg agcgccaccc aatctttggc 361 aatatcgtgg acgaggtggc gtaccatgaa aagtacccaa ccatatatca tctgaggaag 421 aagcttgtag acagtactga taaggctgac ttgcggttga tctatctcgc gctggcgcat 481 atgatcaaat ttcggggaca cttcctcatc gagggggacc tgaacccaga caacagcgat 541 gtcgacaaac tctttatcca actggttcag acttacaatc agcttttcga agagaacccg 601 atcaacgcat ccggagttga cgccaaagca atcctgagcg ctaggctgtc caaatcccgg 661 cggctcgaaa acctcatcgc acagctccct ggggagaaga agaacggcct gtttggtaat 721 cttatcgccc tgtcactcgg gctgaccccc aactttaaat ctaacttcga cctggccgaa 781 gatgccaagc ttcaactgag caaagacacc tacgatgatg atctcgacaa tctgctggcc 841 cagatcggcg accagtacgc agaccttttt ttggcggcaa agaacctgtc agacgccatt 901 ctgctgagtg atattctgcg agtgaacacg gagatcacca aagctccgct gagcgctagt 961 atgatcaagc gctatgatga gcaccaccaa gacttgactt tgctgaaggc ccttgtcaga 1021 cagcaactgc ctgagaagta caaggaaatt ttcttcgatc agtctaaaaa tggctacgcc 1081 ggatacattg acggcggagc aagccaggag gaattttaca aatttattaa gcccatcttg 1141 gaaaaaatgg acggcaccga ggagctgctg gtaaagctta acagagaaga tctgttgcgc 1201 aaacagcgca ctttcgacaa tggaagcatc ccccaccaga ttcacctggg cgaactgcac 1261 gctatcctca ggcggcaaga ggatttctac ccctttttga aagataacag ggaaaagatt 1321 gagaaaatcc tcacatttcg gataccctac tatgtaggcc ccctcgcccg gggaaattcc 1381 agattcgcgt ggatgactcg caaatcagaa gagacaatca ctccctggaa cttcgaggaa 1441 gtcgtggata agggggcctc tgcccagtcc ttcatcgaaa ggatgactaa ctttgataaa 1501 aatctgccta acgaaaaggt gcttcctaaa cactctctgc tgtacgagta cttcacagtt 1561 tataacgagc tcaccaaggt caaatacgtc acagaaggga tgagaaagcc agcattcctg 1621 tctggagagc agaagaaagc tatcgtggac ctcctcttca agacgaaccg gaaagttacc 1681 gtgaaacagc tcaaagaaga ctatttcaaa aagattgaat gtttcgactc tgttgaaatc 1741 agcggagtgg aggatcgctt caacgcatcc ctgggaacgt atcacgatct cctgaaaatc 1801 attaaagaca aggacttcct ggacaatgag gagaacgagg acattcttga ggacattgtc 1861 ctcaccctta cgttgtttga agatagggag atgattgaag aacgcttgaa aacttacgct 1921 catctcttcg acgacaaagt catgaaacag ctcaagaggc gccgatatac aggatggggg 1981 cggctgtcaa gaaaactgat caatgggatc cgagacaagc agagtggaaa gacaatcctg 2041 gattttctta agtccgatgg atttgccaac cggaacttca tgcagttgat ccatgatgac 2101 tctctcacct ttaaggagga catccagaaa gcacaagttt ctggccaggg ggacagtctt 2161 cacgagcaca tcgctaatct tgcaggtagc ccagctatca aaaagggaat actgcagacc 2221 gttaaggtcg tggatgaact cgtcaaagta atgggaaggc ataagcccga gaatatcgtt 2281 atcgagatgg cccgagagaa ccaaactacc cagaagggac agaagaacag tagggaaagg 2341 atgaagagga ttgaagaggg tataaaagaa ctggggtccc aaatccttaa ggaacaccca 2401 gttgaaaaca cccagcttca gaatgagaag ctctacctgt actacctgca gaacggcagg 2461 gacatgtacg tggatcagga actggacatc aatcggctct ccgactacga cgtggatgcc 2521 atcgtgcccc agtcttttct caaagatgat tctattgata ataaagtgtt gacaagatcc 2581 gataaaaata gagggaagag tgataacgtc ccctcagaag aagttgtcaa gaaaatgaaa 2641 aattattggc ggcagctgct gaacgccaaa ctgatcacac aacggaagtt cgataatctg 2701 actaaggctg aacgaggtgg cctgtctgag ttggataaag ccggcttcat caaaaggcag 2761 cttgttgaga cacgccagat caccaagcac gtggcccaaa ttctcgattc acgcatgaac 2821 accaagtacg atgaaaatga caaactgatt cgagaggtga aagttattac tctgaagtct 2881 aagctggtgt cagatttcag aaaggacttt cagttttata aggtgagaga gatcaacaat 2941 taccaccatg cgcatgatgc ctacctgaat gcagtggtag gcactgcact tatcaaaaaa 3001 tatcccaagc ttgaatctga atttgtttac ggagactata aagtgtacga tgttaggaaa 3061 atgatcgcaa agtctgagca ggaaataggc aaggccaccg ctaagtactt cttttacagc 3121 aatattatga attttttcaa gaccgagatt acactggcca atggagagat tcggaagcga 3181 ccacttatcg aaacaaacgg agaaacagga gaaatcgtgt gggacaaggg tagggatttc 3241 gcgacagtcc ggaaggtcct gtccatgccg caggtgaaca tcgttaaaaa gaccgaagta 3301 cagaccggag gcttctccaa ggaaagtatc ctcccgaaaa ggaacagcga caagctgatc 3361 gcacgcaaaa aagattggga ccccaagaaa tacggcggat tcgattctcc tacagtcgct 3421 tacagtgtac tggttgtggc caaagtggag aaagggaagt ctaaaaaact caaaagcgtc 3481 aaggaactgc tgggcatcac aatcatggag cgatcaagct tcgaaaaaaa ccccatcgac 3541 tttctcgagg cgaaaggata taaagaggtc aaaaaagacc tcatcattaa gcttcccaag 3601 tactctctct ttgagcttga aaacggccgg aaacgaatgc tcgctagtgc gggcgagctg 3661 cagaaaggta acgagctggc actgccctct aaatacgtta atttcttgta tctggccagc 3721 cactatgaaa agctcaaagg gtcccccgaa gataatgagc agaagcagct gttcgtggaa 3781 caacacaaac actaccttga tgagatcatc gagcaaataa gcgaattctc caaaagagtg 3841 atcctcgccg acgctaacct cgataaggtg ctttctgctt acaataagca cagggataag 3901 cccatcaggg agcaggcaga aaacattatc cacttgttta ctctgaccaa cttgggcgcg 3961 cctgcagcct tcaagtactt cgacaccacc atagacagaa agcggtacac ctctacaaag 4021 gaggtcctgg acgccacact gattcatcag tcaattacgg ggctctatga aacaagaatc 4081 gacctctctc agctcggtgg agacagcagg gctgacccca agaagaagag gaaggtgtgt 4141 ggtggttctg gtggtggttc tggttgagac ctttggtctc ttgagcttca gtgcaggtga 4201 attctaccac c //