LOCUS BBa_K1179040 4297 bp DNA linear 16-Dec-2024 DEFINITION TRE_Cas9-VP16 ACCESSION BBa_K1179040 VERSION BBa_K1179040.1 FEATURES Location/Qualifiers misc_feature 46..63 /label=TetO Site1 misc_feature 81..98 /label=TetO Site2 misc_feature 117..132 /label=TetO Site3 misc_feature 153..170 /label=TetO Site4 misc_feature 188..205 /label=TetO Site5 misc_feature 224..241 /label=TetO Site6 promoter 1..330 /label=BBa_K415506 misc_feature 332..352 /label=attB1 misc_feature 331..352 /label=BBa_J85600 CDS 353..4297 /label=BBa_K1179009 ORIGIN 1 gctccgaatt cgcccttcag gtccgaggtt ctagacgagt ttactcccta tcagtgatag 61 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct 121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg 181 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag 241 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg 301 aaccgtcaga tcgcaaaggg cgaattcgac ccaagtttgt acaaaaaagc agatgatcaa 361 atttcgggga cacttcctca tcgaggggga cctgaaccca gacaacagcg atgtcgacaa 421 actctttatc caactggttc agacttacaa tcagcttttc gaagagaacc cgatcaacgc 481 atccggagtt gacgccaaag caatcctgag cgctaggctg tccaaatccc ggcggctcga 541 aaacctcatc gcacagctcc ctggggagaa gaagaacggc ctgtttggta atcttatcgc 601 cctgtcactc gggctgaccc ccaactttaa atctaacttc gacctggccg aagatgccaa 661 gcttcaactg agcaaagaca cctacgatga tgatctcgac aatctgctgg cccagatcgg 721 cgaccagtac gcagaccttt ttttggcggc aaagaacctg tcagacgcca ttctgctgag 781 tgatattctg cgagtgaaca cggagatcac caaagctccg ctgagcgcta gtatgatcaa 841 gcgctatgat gagcaccacc aagacttgac tttgctgaag gcccttgtca gacagcaact 901 gcctgagaag tacaaggaaa ttttcttcga tcagtctaaa aatggctacg ccggatacat 961 tgacggcgga gcaagccagg aggaatttta caaatttatt aagcccatct tggaaaaaat 1021 ggacggcacc gaggagctgc tggtaaagct taacagagaa gatctgttgc gcaaacagcg 1081 cactttcgac aatggaagca tcccccacca gattcacctg ggcgaactgc acgctatcct 1141 caggcggcaa gaggatttct accccttttt gaaagataac agggaaaaga ttgagaaaat 1201 cctcacattt cggataccct actatgtagg ccccctcgcc cggggaaatt ccagattcgc 1261 gtggatgact cgcaaatcag aagagacaat cactccctgg aacttcgagg aagtcgtgga 1321 taagggggcc tctgcccagt ccttcatcga aaggatgact aactttgata aaaatctgcc 1381 taacgaaaag gtgcttccta aacactctct gctgtacgag tacttcacag tttataacga 1441 gctcaccaag gtcaaatacg tcacagaagg gatgagaaag ccagcattcc tgtctggaga 1501 gcagaagaaa gctatcgtgg acctcctctt caagacgaac cggaaagtta ccgtgaaaca 1561 gctcaaagaa gactatttca aaaagattga atgtttcgac tctgttgaaa tcagcggagt 1621 ggaggatcgc ttcaacgcat ccctgggaac gtatcacgat ctcctgaaaa tcattaaaga 1681 caaggacttc ctggacaatg aggagaacga ggacattctt gaggacattg tcctcaccct 1741 tacgttgttt gaagataggg agatgattga agaacgcttg aaaacttacg ctcatctctt 1801 cgacgacaaa gtcatgaaac agctcaagag gcgccgatat acaggatggg ggcggctgtc 1861 aagaaaactg atcaatggga tccgagacaa gcagagtgga aagacaatcc tggattttct 1921 taagtccgat ggatttgcca accggaactt catgcagttg atccatgatg actctctcac 1981 ctttaaggag gacatccaga aagcacaagt ttctggccag ggggacagtc ttcacgagca 2041 catcgctaat cttgcaggta gcccagctat caaaaaggga atactgcaga ccgttaaggt 2101 cgtggatgaa ctcgtcaaag taatgggaag gcataagccc gagaatatcg ttatcgagat 2161 ggcccgagag aaccaaacta cccagaaggg acagaagaac agtagggaaa ggatgaagag 2221 gattgaagag ggtataaaag aactggggtc ccaaatcctt aaggaacacc cagttgaaaa 2281 cacccagctt cagaatgaga agctctacct gtactacctg cagaacggca gggacatgta 2341 cgtggatcag gaactggaca tcaatcggct ctccgactac gacgtggatg ccatcgtgcc 2401 ccagtctttt ctcaaagatg attctattga taataaagtg ttgacaagat ccgataaaaa 2461 tagagggaag agtgataacg tcccctcaga agaagttgtc aagaaaatga aaaattattg 2521 gcggcagctg ctgaacgcca aactgatcac acaacggaag ttcgataatc tgactaaggc 2581 tgaacgaggt ggcctgtctg agttggataa agccggcttc atcaaaaggc agcttgttga 2641 gacacgccag atcaccaagc acgtggccca aattctcgat tcacgcatga acaccaagta 2701 cgatgaaaat gacaaactga ttcgagaggt gaaagttatt actctgaagt ctaagctggt 2761 gtcagatttc agaaaggact ttcagtttta taaggtgaga gagatcaaca attaccacca 2821 tgcgcatgat gcctacctga atgcagtggt aggcactgca cttatcaaaa aatatcccaa 2881 gcttgaatct gaatttgttt acggagacta taaagtgtac gatgttagga aaatgatcgc 2941 aaagtctgag caggaaatag gcaaggccac cgctaagtac ttcttttaca gcaatattat 3001 gaattttttc aagaccgaga ttacactggc caatggagag attcggaagc gaccacttat 3061 cgaaacaaac ggagaaacag gagaaatcgt gtgggacaag ggtagggatt tcgcgacagt 3121 ccggaaggtc ctgtccatgc cgcaggtgaa catcgttaaa aagaccgaag tacagaccgg 3181 aggcttctcc aaggaaagta tcctcccgaa aaggaacagc gacaagctga tcgcacgcaa 3241 aaaagattgg gaccccaaga aatacggcgg attcgattct cctacagtcg cttacagtgt 3301 actggttgtg gccaaagtgg agaaagggaa gtctaaaaaa ctcaaaagcg tcaaggaact 3361 gctgggcatc acaatcatgg agcgatcaag cttcgaaaaa aaccccatcg actttctcga 3421 ggcgaaagga tataaagagg tcaaaaaaga cctcatcatt aagcttccca agtactctct 3481 ctttgagctt gaaaacggcc ggaaacgaat gctcgctagt gcgggcgagc tgcagaaagg 3541 taacgagctg gcactgccct ctaaatacgt taatttcttg tatctggcca gccactatga 3601 aaagctcaaa gggtcccccg aagataatga gcagaagcag ctgttcgtgg aacaacacaa 3661 acactacctt gatgagatca tcgagcaaat aagcgaattc tccaaaagag tgatcctcgc 3721 cgacgctaac ctcgataagg tgctttctgc ttacaataag cacagggata agcccatcag 3781 ggagcaggca gaaaacatta tccacttgtt tactctgacc aacttgggcg cgcctgcagc 3841 cttcaagtac ttcgacacca ccatagacag aaagcggtac acctctacaa aggaggtcct 3901 ggacgccaca ctgattcatc agtcaattac ggggctctat gaaacaagaa tcgacctctc 3961 tcagctcggt ggagacagca gggctgaccc caagaagaag aggaaggtgt gtggtggttc 4021 tggtggtggt tctggtatga cccccccgac cgatgtcagc ctgggggacg agctccactt 4081 agacggcgag gacgtggcga tggcgcatgc cgacgcgcta gacgatttcg atctggacat 4141 gttgggggac ggggattccc cgggtccggg atttaccccc cacgactccg ccccctacgg 4201 cgctctggat atggccgact tcgagtttga gcagatgttt accgatgccc ttggaattga 4261 cgagtacggt gggacgcgtg agcttcagtg caggtga //