LOCUS BBa_K1179051 4510 bp DNA linear 23-Dec-2024 DEFINITION VC155-Cas9(D10A and H840A mutant) ACCESSION BBa_K1179051 VERSION BBa_K1179051.1 FEATURES Location/Qualifiers ORIGIN 1 ggatcccggt accctcgaca cctgctgaca ggtccaccat ggacaagcag aagaacggca 61 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc 121 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc 181 tgagctacca gtccaaactg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc 241 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagaatggtg 301 gttctggtgg tggttcggac aagaagtact ccattgggct cgctatcggc acaaacagcg 361 tcggctgggc cgtcattacg gacgagtaca aggtgccgag caaaaaattc aaagttctgg 421 gcaataccga tcgccacagc ataaagaaga acctcattgg cgccctcctg ttcgactccg 481 gggagacggc cgaagccacg cggctcaaaa gaacagcacg gcgcagatat acccgcagaa 541 agaatcggat ctgctacctg caggagatct ttagtaatga gatggctaag gtggatgact 601 ctttcttcca taggctggag gagtcctttt tggtggagga ggataaaaag cacgagcgcc 661 acccaatctt tggcaatatc gtggacgagg tggcgtacca tgaaaagtac ccaaccatat 721 atcatctgag gaagaagctt gtagacagta ctgataaggc tgacttgcgg ttgatctatc 781 tcgcgctggc gcatatgatc aaatttcggg gacacttcct catcgagggg gacctgaacc 841 cagacaacag cgatgtcgac aaactcttta tccaactggt tcagacttac aatcagcttt 901 tcgaagagaa cccgatcaac gcatccggag ttgacgccaa agcaatcctg agcgctaggc 961 tgtccaaatc ccggcggctc gaaaacctca tcgcacagct ccctggggag aagaagaacg 1021 gcctgtttgg taatcttatc gccctgtcac tcgggctgac ccccaacttt aaatctaact 1081 tcgacctggc cgaagatgcc aagcttcaac tgagcaaaga cacctacgat gatgatctcg 1141 acaatctgct ggcccagatc ggcgaccagt acgcagacct ttttttggcg gcaaagaacc 1201 tgtcagacgc cattctgctg agtgatattc tgcgagtgaa cacggagatc accaaagctc 1261 cgctgagcgc tagtatgatc aagcgctatg atgagcacca ccaagacttg actttgctga 1321 aggcccttgt cagacagcaa ctgcctgaga agtacaagga aattttcttc gatcagtcta 1381 aaaatggcta cgccggatac attgacggcg gagcaagcca ggaggaattt tacaaattta 1441 ttaagcccat cttggaaaaa atggacggca ccgaggagct gctggtaaag cttaacagag 1501 aagatctgtt gcgcaaacag cgcactttcg acaatggaag catcccccac cagattcacc 1561 tgggcgaact gcacgctatc ctcaggcggc aagaggattt ctaccccttt ttgaaagata 1621 acagggaaaa gattgagaaa atcctcacat ttcggatacc ctactatgta ggccccctcg 1681 cccggggaaa ttccagattc gcgtggatga ctcgcaaatc agaagagaca atcactccct 1741 ggaacttcga ggaagtcgtg gataaggggg cctctgccca gtccttcatc gaaaggatga 1801 ctaactttga taaaaatctg cctaacgaaa aggtgcttcc taaacactct ctgctgtacg 1861 agtacttcac agtttataac gagctcacca aggtcaaata cgtcacagaa gggatgagaa 1921 agccagcatt cctgtctgga gagcagaaga aagctatcgt ggacctcctc ttcaagacga 1981 accggaaagt taccgtgaaa cagctcaaag aagactattt caaaaagatt gaatgtttcg 2041 actctgttga aatcagcgga gtggaggatc gcttcaacgc atccctggga acgtatcacg 2101 atctcctgaa aatcattaaa gacaaggact tcctggacaa tgaggagaac gaggacattc 2161 ttgaggacat tgtcctcacc cttacgttgt ttgaagatag ggagatgatt gaagaacgct 2221 tgaaaactta cgctcatctc ttcgacgaca aagtcatgaa acagctcaag aggcgccgat 2281 atacaggatg ggggcggctg tcaagaaaac tgatcaatgg gatccgagac aagcagagtg 2341 gaaagacaat cctggatttt cttaagtccg atggatttgc caaccggaac ttcatgcagt 2401 tgatccatga tgactctctc acctttaagg aggacatcca gaaagcacaa gtttctggcc 2461 agggggacag tcttcacgag cacatcgcta atcttgcagg tagcccagct atcaaaaagg 2521 gaatactgca gaccgttaag gtcgtggatg aactcgtcaa agtaatggga aggcataagc 2581 ccgagaatat cgttatcgag atggcccgag agaaccaaac tacccagaag ggacagaaga 2641 acagtaggga aaggatgaag aggattgaag agggtataaa agaactgggg tcccaaatcc 2701 ttaaggaaca cccagttgaa aacacccagc ttcagaatga gaagctctac ctgtactacc 2761 tgcagaacgg cagggacatg tacgtggatc aggaactgga catcaatcgg ctctccgact 2821 acgacgtgga tgccatcgtg ccccagtctt ttctcaaaga tgattctatt gataataaag 2881 tgttgacaag atccgataaa aatagaggga agagtgataa cgtcccctca gaagaagttg 2941 tcaagaaaat gaaaaattat tggcggcagc tgctgaacgc caaactgatc acacaacgga 3001 agttcgataa tctgactaag gctgaacgag gtggcctgtc tgagttggat aaagccggct 3061 tcatcaaaag gcagcttgtt gagacacgcc agatcaccaa gcacgtggcc caaattctcg 3121 attcacgcat gaacaccaag tacgatgaaa atgacaaact gattcgagag gtgaaagtta 3181 ttactctgaa gtctaagctg gtgtcagatt tcagaaagga ctttcagttt tataaggtga 3241 gagagatcaa caattaccac catgcgcatg atgcctacct gaatgcagtg gtaggcactg 3301 cacttatcaa aaaatatccc aagcttgaat ctgaatttgt ttacggagac tataaagtgt 3361 acgatgttag gaaaatgatc gcaaagtctg agcaggaaat aggcaaggcc accgctaagt 3421 acttctttta cagcaatatt atgaattttt tcaagaccga gattacactg gccaatggag 3481 agattcggaa gcgaccactt atcgaaacaa acggagaaac aggagaaatc gtgtgggaca 3541 agggtaggga tttcgcgaca gtccggaagg tcctgtccat gccgcaggtg aacatcgtta 3601 aaaagaccga agtacagacc ggaggcttct ccaaggaaag tatcctcccg aaaaggaaca 3661 gcgacaagct gatcgcacgc aaaaaagatt gggaccccaa gaaatacggc ggattcgatt 3721 ctcctacagt cgcttacagt gtactggttg tggccaaagt ggagaaaggg aagtctaaaa 3781 aactcaaaag cgtcaaggaa ctgctgggca tcacaatcat ggagcgatca agcttcgaaa 3841 aaaaccccat cgactttctc gaggcgaaag gatataaaga ggtcaaaaaa gacctcatca 3901 ttaagcttcc caagtactct ctctttgagc ttgaaaacgg ccggaaacga atgctcgcta 3961 gtgcgggcga gctgcagaaa ggtaacgagc tggcactgcc ctctaaatac gttaatttct 4021 tgtatctggc cagccactat gaaaagctca aagggtcccc cgaagataat gagcagaagc 4081 agctgttcgt ggaacaacac aaacactacc ttgatgagat catcgagcaa ataagcgaat 4141 tctccaaaag agtgatcctc gccgacgcta acctcgataa ggtgctttct gcttacaata 4201 agcacaggga taagcccatc agggagcagg cagaaaacat tatccacttg tttactctga 4261 ccaacttggg cgcgcctgca gccttcaagt acttcgacac caccatagac agaaagcggt 4321 acacctctac aaaggaggtc ctggacgcca cactgattca tcagtcaatt acggggctct 4381 atgaaacaag aatcgacctc tctcagctcg gtggagacag cagggctgac cccaagaaga 4441 agaggaaggt gtgaaagggt tcgatcccta ccggttagta atggcttcag tgcaggtgaa 4501 ttctaccacc //