LOCUS BBa_K1179054 4720 bp DNA linear 26-Nov-2024 DEFINITION VN155-Cas9(D10A and H840A mutant) ACCESSION BBa_K1179054 VERSION BBa_K1179054.1 FEATURES Location/Qualifiers ORIGIN 1 ggatcccggt accctcgaca cctgctgaca ggtccaccat ggtgagcaag ggcgaggagc 61 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt 121 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagctga 181 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgggctacg 241 gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg 301 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca 361 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg 421 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca 481 gccacaacgc gtatatcacc gccaatggtg gttctggtgg tggttcggac aagaagtact 541 ccattgggct cgctatcggc acaaacagcg tcggctgggc cgtcattacg gacgagtaca 601 aggtgccgag caaaaaattc aaagttctgg gcaataccga tcgccacagc ataaagaaga 661 acctcattgg cgccctcctg ttcgactccg gggagacggc cgaagccacg cggctcaaaa 721 gaacagcacg gcgcagatat acccgcagaa agaatcggat ctgctacctg caggagatct 781 ttagtaatga gatggctaag gtggatgact ctttcttcca taggctggag gagtcctttt 841 tggtggagga ggataaaaag cacgagcgcc acccaatctt tggcaatatc gtggacgagg 901 tggcgtacca tgaaaagtac ccaaccatat atcatctgag gaagaagctt gtagacagta 961 ctgataaggc tgacttgcgg ttgatctatc tcgcgctggc gcatatgatc aaatttcggg 1021 gacacttcct catcgagggg gacctgaacc cagacaacag cgatgtcgac aaactcttta 1081 tccaactggt tcagacttac aatcagcttt tcgaagagaa cccgatcaac gcatccggag 1141 ttgacgccaa agcaatcctg agcgctaggc tgtccaaatc ccggcggctc gaaaacctca 1201 tcgcacagct ccctggggag aagaagaacg gcctgtttgg taatcttatc gccctgtcac 1261 tcgggctgac ccccaacttt aaatctaact tcgacctggc cgaagatgcc aagcttcaac 1321 tgagcaaaga cacctacgat gatgatctcg acaatctgct ggcccagatc ggcgaccagt 1381 acgcagacct ttttttggcg gcaaagaacc tgtcagacgc cattctgctg agtgatattc 1441 tgcgagtgaa cacggagatc accaaagctc cgctgagcgc tagtatgatc aagcgctatg 1501 atgagcacca ccaagacttg actttgctga aggcccttgt cagacagcaa ctgcctgaga 1561 agtacaagga aattttcttc gatcagtcta aaaatggcta cgccggatac attgacggcg 1621 gagcaagcca ggaggaattt tacaaattta ttaagcccat cttggaaaaa atggacggca 1681 ccgaggagct gctggtaaag cttaacagag aagatctgtt gcgcaaacag cgcactttcg 1741 acaatggaag catcccccac cagattcacc tgggcgaact gcacgctatc ctcaggcggc 1801 aagaggattt ctaccccttt ttgaaagata acagggaaaa gattgagaaa atcctcacat 1861 ttcggatacc ctactatgta ggccccctcg cccggggaaa ttccagattc gcgtggatga 1921 ctcgcaaatc agaagagaca atcactccct ggaacttcga ggaagtcgtg gataaggggg 1981 cctctgccca gtccttcatc gaaaggatga ctaactttga taaaaatctg cctaacgaaa 2041 aggtgcttcc taaacactct ctgctgtacg agtacttcac agtttataac gagctcacca 2101 aggtcaaata cgtcacagaa gggatgagaa agccagcatt cctgtctgga gagcagaaga 2161 aagctatcgt ggacctcctc ttcaagacga accggaaagt taccgtgaaa cagctcaaag 2221 aagactattt caaaaagatt gaatgtttcg actctgttga aatcagcgga gtggaggatc 2281 gcttcaacgc atccctggga acgtatcacg atctcctgaa aatcattaaa gacaaggact 2341 tcctggacaa tgaggagaac gaggacattc ttgaggacat tgtcctcacc cttacgttgt 2401 ttgaagatag ggagatgatt gaagaacgct tgaaaactta cgctcatctc ttcgacgaca 2461 aagtcatgaa acagctcaag aggcgccgat atacaggatg ggggcggctg tcaagaaaac 2521 tgatcaatgg gatccgagac aagcagagtg gaaagacaat cctggatttt cttaagtccg 2581 atggatttgc caaccggaac ttcatgcagt tgatccatga tgactctctc acctttaagg 2641 aggacatcca gaaagcacaa gtttctggcc agggggacag tcttcacgag cacatcgcta 2701 atcttgcagg tagcccagct atcaaaaagg gaatactgca gaccgttaag gtcgtggatg 2761 aactcgtcaa agtaatggga aggcataagc ccgagaatat cgttatcgag atggcccgag 2821 agaaccaaac tacccagaag ggacagaaga acagtaggga aaggatgaag aggattgaag 2881 agggtataaa agaactgggg tcccaaatcc ttaaggaaca cccagttgaa aacacccagc 2941 ttcagaatga gaagctctac ctgtactacc tgcagaacgg cagggacatg tacgtggatc 3001 aggaactgga catcaatcgg ctctccgact acgacgtgga tgccatcgtg ccccagtctt 3061 ttctcaaaga tgattctatt gataataaag tgttgacaag atccgataaa aatagaggga 3121 agagtgataa cgtcccctca gaagaagttg tcaagaaaat gaaaaattat tggcggcagc 3181 tgctgaacgc caaactgatc acacaacgga agttcgataa tctgactaag gctgaacgag 3241 gtggcctgtc tgagttggat aaagccggct tcatcaaaag gcagcttgtt gagacacgcc 3301 agatcaccaa gcacgtggcc caaattctcg attcacgcat gaacaccaag tacgatgaaa 3361 atgacaaact gattcgagag gtgaaagtta ttactctgaa gtctaagctg gtgtcagatt 3421 tcagaaagga ctttcagttt tataaggtga gagagatcaa caattaccac catgcgcatg 3481 atgcctacct gaatgcagtg gtaggcactg cacttatcaa aaaatatccc aagcttgaat 3541 ctgaatttgt ttacggagac tataaagtgt acgatgttag gaaaatgatc gcaaagtctg 3601 agcaggaaat aggcaaggcc accgctaagt acttctttta cagcaatatt atgaattttt 3661 tcaagaccga gattacactg gccaatggag agattcggaa gcgaccactt atcgaaacaa 3721 acggagaaac aggagaaatc gtgtgggaca agggtaggga tttcgcgaca gtccggaagg 3781 tcctgtccat gccgcaggtg aacatcgtta aaaagaccga agtacagacc ggaggcttct 3841 ccaaggaaag tatcctcccg aaaaggaaca gcgacaagct gatcgcacgc aaaaaagatt 3901 gggaccccaa gaaatacggc ggattcgatt ctcctacagt cgcttacagt gtactggttg 3961 tggccaaagt ggagaaaggg aagtctaaaa aactcaaaag cgtcaaggaa ctgctgggca 4021 tcacaatcat ggagcgatca agcttcgaaa aaaaccccat cgactttctc gaggcgaaag 4081 gatataaaga ggtcaaaaaa gacctcatca ttaagcttcc caagtactct ctctttgagc 4141 ttgaaaacgg ccggaaacga atgctcgcta gtgcgggcga gctgcagaaa ggtaacgagc 4201 tggcactgcc ctctaaatac gttaatttct tgtatctggc cagccactat gaaaagctca 4261 aagggtcccc cgaagataat gagcagaagc agctgttcgt ggaacaacac aaacactacc 4321 ttgatgagat catcgagcaa ataagcgaat tctccaaaag agtgatcctc gccgacgcta 4381 acctcgataa ggtgctttct gcttacaata agcacaggga taagcccatc agggagcagg 4441 cagaaaacat tatccacttg tttactctga ccaacttggg cgcgcctgca gccttcaagt 4501 acttcgacac caccatagac agaaagcggt acacctctac aaaggaggtc ctggacgcca 4561 cactgattca tcagtcaatt acggggctct atgaaacaag aatcgacctc tctcagctcg 4621 gtggagacag cagggctgac cccaagaaga agaggaaggt gtgaaagggt tcgatcccta 4681 ccggttagta atggcttcag tgcaggtgaa ttctaccacc //