LOCUS BBa_K1179057 4774 bp DNA linear 23-Dec-2024 DEFINITION VN173-Cas9(D10A and H840A mutant) ACCESSION BBa_K1179057 VERSION BBa_K1179057.1 FEATURES Location/Qualifiers ORIGIN 1 ggatcccggt accctcgaca cctgctgaca ggtccaccat ggtgagcaag ggcgaggagc 61 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt 121 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagctga 181 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgggctacg 241 gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg 301 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca 361 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg 421 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca 481 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga 541 tccgccacaa catcgagaat ggtggttctg gtggtggttc ggacaagaag tactccattg 601 ggctcgctat cggcacaaac agcgtcggct gggccgtcat tacggacgag tacaaggtgc 661 cgagcaaaaa attcaaagtt ctgggcaata ccgatcgcca cagcataaag aagaacctca 721 ttggcgccct cctgttcgac tccggggaga cggccgaagc cacgcggctc aaaagaacag 781 cacggcgcag atatacccgc agaaagaatc ggatctgcta cctgcaggag atctttagta 841 atgagatggc taaggtggat gactctttct tccataggct ggaggagtcc tttttggtgg 901 aggaggataa aaagcacgag cgccacccaa tctttggcaa tatcgtggac gaggtggcgt 961 accatgaaaa gtacccaacc atatatcatc tgaggaagaa gcttgtagac agtactgata 1021 aggctgactt gcggttgatc tatctcgcgc tggcgcatat gatcaaattt cggggacact 1081 tcctcatcga gggggacctg aacccagaca acagcgatgt cgacaaactc tttatccaac 1141 tggttcagac ttacaatcag cttttcgaag agaacccgat caacgcatcc ggagttgacg 1201 ccaaagcaat cctgagcgct aggctgtcca aatcccggcg gctcgaaaac ctcatcgcac 1261 agctccctgg ggagaagaag aacggcctgt ttggtaatct tatcgccctg tcactcgggc 1321 tgacccccaa ctttaaatct aacttcgacc tggccgaaga tgccaagctt caactgagca 1381 aagacaccta cgatgatgat ctcgacaatc tgctggccca gatcggcgac cagtacgcag 1441 accttttttt ggcggcaaag aacctgtcag acgccattct gctgagtgat attctgcgag 1501 tgaacacgga gatcaccaaa gctccgctga gcgctagtat gatcaagcgc tatgatgagc 1561 accaccaaga cttgactttg ctgaaggccc ttgtcagaca gcaactgcct gagaagtaca 1621 aggaaatttt cttcgatcag tctaaaaatg gctacgccgg atacattgac ggcggagcaa 1681 gccaggagga attttacaaa tttattaagc ccatcttgga aaaaatggac ggcaccgagg 1741 agctgctggt aaagcttaac agagaagatc tgttgcgcaa acagcgcact ttcgacaatg 1801 gaagcatccc ccaccagatt cacctgggcg aactgcacgc tatcctcagg cggcaagagg 1861 atttctaccc ctttttgaaa gataacaggg aaaagattga gaaaatcctc acatttcgga 1921 taccctacta tgtaggcccc ctcgcccggg gaaattccag attcgcgtgg atgactcgca 1981 aatcagaaga gacaatcact ccctggaact tcgaggaagt cgtggataag ggggcctctg 2041 cccagtcctt catcgaaagg atgactaact ttgataaaaa tctgcctaac gaaaaggtgc 2101 ttcctaaaca ctctctgctg tacgagtact tcacagttta taacgagctc accaaggtca 2161 aatacgtcac agaagggatg agaaagccag cattcctgtc tggagagcag aagaaagcta 2221 tcgtggacct cctcttcaag acgaaccgga aagttaccgt gaaacagctc aaagaagact 2281 atttcaaaaa gattgaatgt ttcgactctg ttgaaatcag cggagtggag gatcgcttca 2341 acgcatccct gggaacgtat cacgatctcc tgaaaatcat taaagacaag gacttcctgg 2401 acaatgagga gaacgaggac attcttgagg acattgtcct cacccttacg ttgtttgaag 2461 atagggagat gattgaagaa cgcttgaaaa cttacgctca tctcttcgac gacaaagtca 2521 tgaaacagct caagaggcgc cgatatacag gatgggggcg gctgtcaaga aaactgatca 2581 atgggatccg agacaagcag agtggaaaga caatcctgga ttttcttaag tccgatggat 2641 ttgccaaccg gaacttcatg cagttgatcc atgatgactc tctcaccttt aaggaggaca 2701 tccagaaagc acaagtttct ggccaggggg acagtcttca cgagcacatc gctaatcttg 2761 caggtagccc agctatcaaa aagggaatac tgcagaccgt taaggtcgtg gatgaactcg 2821 tcaaagtaat gggaaggcat aagcccgaga atatcgttat cgagatggcc cgagagaacc 2881 aaactaccca gaagggacag aagaacagta gggaaaggat gaagaggatt gaagagggta 2941 taaaagaact ggggtcccaa atccttaagg aacacccagt tgaaaacacc cagcttcaga 3001 atgagaagct ctacctgtac tacctgcaga acggcaggga catgtacgtg gatcaggaac 3061 tggacatcaa tcggctctcc gactacgacg tggatgccat cgtgccccag tcttttctca 3121 aagatgattc tattgataat aaagtgttga caagatccga taaaaataga gggaagagtg 3181 ataacgtccc ctcagaagaa gttgtcaaga aaatgaaaaa ttattggcgg cagctgctga 3241 acgccaaact gatcacacaa cggaagttcg ataatctgac taaggctgaa cgaggtggcc 3301 tgtctgagtt ggataaagcc ggcttcatca aaaggcagct tgttgagaca cgccagatca 3361 ccaagcacgt ggcccaaatt ctcgattcac gcatgaacac caagtacgat gaaaatgaca 3421 aactgattcg agaggtgaaa gttattactc tgaagtctaa gctggtgtca gatttcagaa 3481 aggactttca gttttataag gtgagagaga tcaacaatta ccaccatgcg catgatgcct 3541 acctgaatgc agtggtaggc actgcactta tcaaaaaata tcccaagctt gaatctgaat 3601 ttgtttacgg agactataaa gtgtacgatg ttaggaaaat gatcgcaaag tctgagcagg 3661 aaataggcaa ggccaccgct aagtacttct tttacagcaa tattatgaat tttttcaaga 3721 ccgagattac actggccaat ggagagattc ggaagcgacc acttatcgaa acaaacggag 3781 aaacaggaga aatcgtgtgg gacaagggta gggatttcgc gacagtccgg aaggtcctgt 3841 ccatgccgca ggtgaacatc gttaaaaaga ccgaagtaca gaccggaggc ttctccaagg 3901 aaagtatcct cccgaaaagg aacagcgaca agctgatcgc acgcaaaaaa gattgggacc 3961 ccaagaaata cggcggattc gattctccta cagtcgctta cagtgtactg gttgtggcca 4021 aagtggagaa agggaagtct aaaaaactca aaagcgtcaa ggaactgctg ggcatcacaa 4081 tcatggagcg atcaagcttc gaaaaaaacc ccatcgactt tctcgaggcg aaaggatata 4141 aagaggtcaa aaaagacctc atcattaagc ttcccaagta ctctctcttt gagcttgaaa 4201 acggccggaa acgaatgctc gctagtgcgg gcgagctgca gaaaggtaac gagctggcac 4261 tgccctctaa atacgttaat ttcttgtatc tggccagcca ctatgaaaag ctcaaagggt 4321 cccccgaaga taatgagcag aagcagctgt tcgtggaaca acacaaacac taccttgatg 4381 agatcatcga gcaaataagc gaattctcca aaagagtgat cctcgccgac gctaacctcg 4441 ataaggtgct ttctgcttac aataagcaca gggataagcc catcagggag caggcagaaa 4501 acattatcca cttgtttact ctgaccaact tgggcgcgcc tgcagccttc aagtacttcg 4561 acaccaccat agacagaaag cggtacacct ctacaaagga ggtcctggac gccacactga 4621 ttcatcagtc aattacgggg ctctatgaaa caagaatcga cctctctcag ctcggtggag 4681 acagcagggc tgaccccaag aagaagagga aggtgtgaaa gggttcgatc cctaccggtt 4741 agtaatggct tcagtgcagg tgaattctac cacc //