LOCUS BBa_K1179073 1241 bp DNA linear 25-Nov-2024 DEFINITION TRE-tight_eYFP-4x451 ACCESSION BBa_K1179073 VERSION BBa_K1179073.1 FEATURES Location/Qualifiers misc_feature 46..63 /label=TetO Site 1 misc_feature 117..134 /label=TetO Site 3 misc_feature 81..98 /label=TetO Site 2 misc_feature 153..170 /label=TetO Site 4 misc_feature 188..205 /label=TetO Site 5 misc_feature 224..241 /label=TetO Site 6 promoter 256..313 /label=CMV-minimal promoter 1..330 /label=BBa_K1179078 misc_feature 332..352 /label=attB1 misc_feature 331..352 /label=BBa_J85600 CDS 353..1241 /label=BBa_K1179060 ORIGIN 1 gctccgaatt cgcccttcag gtccgaggtt ctagacgagt ttactcccta tcagtgatag 61 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct 121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg 181 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag 241 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg 301 aaccgtcaga tcgcaaaggg cgaattcgac ccaagtttgt acaaaaaagc agggatcccg 361 gtaccctcga cacctgctga caggtatggt gagcaagggc gaggagctgt tcaccggggt 421 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg 481 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg 541 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcagtgctt 601 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg 661 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga 721 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa 781 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta 841 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat 901 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg 961 ccccgtgctg ctgcccgaca accactacct gagctaccag tccaagctga gcaaagaccc 1021 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct 1081 cggcatggac gagctgtaca agtaaagcgg cgcgccaact cagtaatggt aacggtttaa 1141 ctcagtaatg gtaacggttt aactcagtaa tggtaacggt ttaactcagt aatggtaacg 1201 gtttactagt taccgcttca gtgcaggtga attctaccac c //